Peripheral blood buffy coat smear: a promising tool for diagnosis of visceral leishmaniasis.

abstract：:Virology laboratories historically have used direct fluorescent-antibody assay (DFA) and culture to detect six or seven respiratory viruses. Following the discovery of five new human respiratory viruses since 2000, there is an increasing need for diagnostic tests to detect these emerging viruses. We have developed a n...

journal_title：Journal of clinical microbiology

authors： Mahony J,Chong S,Merante F,Yaghoubian S,Sinha T,Lisle C,Janeczko R

更新日期：2007-09-01 00:00:00

abstract：:The use of IS6110 as a marker for molecular epidemiological studies is limited when a Mycobacterium tuberculosis isolate has five or fewer copies of IS6110. Restriction fragment length polymorphism analysis with a highly polymorphic GC-rich repetitive sequence located in the plasmid pTBN12 (PGRS RFLP) and spoligotypin...

journal_title：Journal of clinical microbiology

authors： Flores L,Jarlsberg LG,Kim EY,Osmond D,Grinsdale J,Kawamura M,Desmond E,Hopewell PC,Kato-Maeda M

更新日期：2010-02-01 00:00:00

abstract：:Sequence analysis of the penA gene, encoding penicillin-binding protein 2 (PBP2), in 30 penicillin-intermediate (PenI) Neisseria meningitidis strains showed altered gene sequences due to the translocation of exogenous DNA blocks derived from commensal neisseriae, which are known to have PBP2 proteins with decreased af...

journal_title：Journal of clinical microbiology

authors： Stefanelli P,Carattoli A,Neri A,Fazio C,Mastrantonio P

更新日期：2003-10-01 00:00:00

abstract：:Reagent-impregnated filter paper replica methods are described which can be used to detect all penicillinase (beta-lactamase)-producing Neisseria gonorrhoeae (PPNG) colonies in an entire population of Neisseria gonorrhoeae growing on primary isolation plates. ...

journal_title：Journal of clinical microbiology

authors： Bae BH,Ledesma G,Korzis J

更新日期：1983-03-01 00:00:00

abstract：:We used the slide agglutination test to determine the serologic relationships of 22 Legionella spp. representing 33 serogroups. Antisera prepared against 14 of the Legionella spp. contained cross-reactive antibodies (1+ or greater) at their working dilutions. Numerous cross-reactions were observed for the blue-white f...

journal_title：Journal of clinical microbiology

authors： Thacker WL,Plikaytis BB,Wilkinson HW

更新日期：1985-05-01 00:00:00

abstract：:The recent Zaire Ebola virus (EBOV) outbreak in West Africa illustrates clearly the need for additional studies with humans and animals to elucidate the ecology of Ebola viruses (EBVs). In this study, we developed a serological assay based on the Luminex technology. Nine recombinant proteins representing different vir...

journal_title：Journal of clinical microbiology

authors： Ayouba A,Touré A,Butel C,Keita AK,Binetruy F,Sow MS,Foulongne V,Delaporte E,Peeters M

更新日期：2016-12-28 00:00:00

abstract：:A technique is described which may be used to identify Haemophilus influenzae type b, Streptococcus pneumoniae, group A and B streptococci, and Staphylococcus aureus in smears of clinical specimens. ...

journal_title：Journal of clinical microbiology

authors： Clausen CR

更新日期：1981-06-01 00:00:00

abstract：:To define the optimal conditions for human immunodeficiency virus (HIV) detection in microcultures, experiments were conducted with different ratios of patient and donor peripheral blood mononuclear cells (PBMCs). Donor/patient PBMC ratios ranged from 1:1 to 1:125. Optimal results were obtained when 1,500,000 donor ce...

journal_title：Journal of clinical microbiology

authors： Dimitrov DH,Melnick JL,Hollinger FB

更新日期：1990-04-01 00:00:00

abstract：:The causative agent of Johne's disease is Mycobacterium avium subsp. paratuberculosis. This is a chronic, debilitating gastrointestinal disorder that affects ruminants and is responsible for significant economic loss. The specimen processing method that combines C(18)-carboxypropylbetaine (CB-18) treatment and lytic e...

journal_title：Journal of clinical microbiology

authors： Thornton CG,MacLellan KM,Stabel JR,Carothers C,Whitlock RH,Passen S

更新日期：2002-05-01 00:00:00

abstract：:The human herpesvirus 6 (HHV-6) early protein, p41, encoded by the U27 gene has been detected in oligodendrocytes of multiple sclerosis (MS) patients by using a monoclonal antibody (MAb to p41/38). We here report the antigenic epitope of HHV-6 p41 recognized by this MAb. First, we established that the MAb to p41/38 re...

journal_title：Journal of clinical microbiology

authors： Xu Y,Linde A,Dahl H,Winberg G

更新日期：2001-04-01 00:00:00

abstract：:Two molecular fingerprinting techniques, pulsed-field gel electrophoresis (PFGE) and amplified fragment length polymorphism (AFLP), were used to investigate the epidemiological relatedness among Clostridium difficile isolates from suspected outbreaks in three general hospitals. Analysis by PFGE yielded inconclusive da...

journal_title：Journal of clinical microbiology

authors： Klaassen CH,van Haren HA,Horrevorts AM

更新日期：2002-01-01 00:00:00

abstract：:A total of 221 isolates of multidrug-resistant Salmonella enterica serovar Typhimurium in Japan were characterized in the present study. The results revealed that clonal serovar Typhimurium definitive phage type 104 strains prevailed and that these strains had drug resistance patterns, integron types, and pulsed-field...

journal_title：Journal of clinical microbiology

authors： Izumiya H,Terajima J,Matsushita S,Tamura K,Watanabe H

更新日期：2001-07-01 00:00:00

abstract：:In a prospective study of 16 patients with Legionnaires disease confirmed by cultural isolation of Legionella pneumophila from the respiratory tract, 38% (6 of 16) had positive blood cultures. Daily subcultures were made onto buffered charcoal-yeast extract plates from 6B aerobic and 7C anaerobic BACTEC blood culture ...

journal_title：Journal of clinical microbiology

authors： Rihs JD,Yu VL,Zuravleff JJ,Goetz A,Muder RR

更新日期：1985-09-01 00:00:00

abstract：:Unbiased nontargeted metagenomic RNA sequencing (UMERS) has the advantage to detect known as well as unknown pathogens and, thus, can significantly improve the detection of viral, bacterial, parasitic, and fungal sequences in public health settings. In particular, conventional diagnostic methods successfully identify ...

journal_title：Journal of clinical microbiology

authors： Fischer N,Indenbirken D,Meyer T,Lütgehetmann M,Lellek H,Spohn M,Aepfelbacher M,Alawi M,Grundhoff A

更新日期：2015-07-01 00:00:00

abstract：:In the last 2 decades, a variety of different molecular typing methods have been developed to differentiate strains of Mycobacterium avium subsp. paratuberculosis. The most successful techniques are based on insertion sequences, repetitive loci, comparative genomics, or single nucleotide polymorphisms. In the present ...

journal_title：Journal of clinical microbiology

authors： Griffiths TA,Rioux K,De Buck J

更新日期：2008-04-01 00:00:00

abstract：:Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of nosocomial pneumonia. To characterize pathogen-derived and host-related factors in intensive care unit (ICU) patients with MRSA pneumonia, we evaluated the Improving Medicine through Pathway Assessment of Critical Therapy in Hospital-Acquired Pneum...

journal_title：Journal of clinical microbiology

authors： Haque NZ,Arshad S,Peyrani P,Ford KD,Perri MB,Jacobsen G,Reyes K,Scerpella EG,Ramirez JA,Zervos MJ

更新日期：2012-05-01 00:00:00

abstract：:We report the isolation of Pseudomonas putrefaciens from an intra-abdominal abscess in a patient with colonic carcinoma and from bile in two patients with biliary tract disease. In all three cases, P. putrefaciens was isolated in mixed culture with enteric bacteria. ...

journal_title：Journal of clinical microbiology

authors： Marne C,Pallarés R,Sitges-Serra A

更新日期：1983-06-01 00:00:00

abstract：:Respiratory diphtheria, characterized by a firmly adherent pseudomembrane, is caused by toxin-producing strains of Corynebacterium diphtheriae, with similar illness produced occasionally by toxigenic Corynebacterium ulcerans or, rarely, Corynebacterium pseudotuberculosis While diphtheria laboratory confirmation requir...

journal_title：Journal of clinical microbiology

authors： Williams MM,Waller JL,Aneke JS,Weigand MR,Diaz MH,Bowden KE,Simon AK,Peng Y,Xiaoli L,Cassiday PK,Winchell J,Tondella ML

更新日期：2020-09-22 00:00:00

abstract：:A total of 513 blood specimens, predominantly from organ transplant recipients, human immunodeficiency virus-positive patients, and bone marrow transplant recipients, were tested for cytomegalovirus (CMV) by culture and pp65 antigenemia across four test sites. Peripheral blood leukocytes were examined by using both th...

journal_title：Journal of clinical microbiology

authors： St George K,Boyd MJ,Lipson SM,Ferguson D,Cartmell GF,Falk LH,Rinaldo CR,Landry ML

更新日期：2000-04-01 00:00:00

abstract：:A total of 151 Escherichia coli strains resistant to cefotaxime and ceftazidime were isolated during a prospective surveillance study. These strains were characterized by clinical, microbiological, and molecular analyses and were distributed into four clusters of 103, 11, 6, and 5 isolates, along with 25 unrelated str...

journal_title：Journal of clinical microbiology

authors： Oteo J,Navarro C,Cercenado E,Delgado-Iribarren A,Wilhelmi I,Orden B,García C,Miguelañez S,Pérez-Vázquez M,García-Cobos S,Aracil B,Bautista V,Campos J

更新日期：2006-07-01 00:00:00

abstract：:We reviewed laboratory-acquired infections occurring in Utah from 1978 through 1982. Written and telephone interviews of supervisors of 1,191 laboratorians revealed an estimated annual incidence of 3 laboratory-acquired infections per 1,000 employees. Infections, in order of frequency, included hepatitis B (clinical c...

journal_title：Journal of clinical microbiology

authors： Jacobson JT,Orlob RB,Clayton JL

更新日期：1985-04-01 00:00:00

abstract：:We studied the plasmid profiles of 136 Shigella isolates in Bangladesh to determine whether plasmid profiles could be used for differentiation of strains for epidemiological studies. Many different plasmid patterns were observed within each species, indicating that many genetically different strains of Shigella are re...

journal_title：Journal of clinical microbiology

authors： Tacket CO,Shahid N,Huq MI,Alim AR,Cohen ML

更新日期：1984-08-01 00:00:00

abstract：:Capnocytophaga ochracea is a gram-negative, fusiform bacillus which is part of the normal human oral flora. This organism is often isolated from periodontal lesions of patients with periodontitis and is associated with sepsis in granulocytopenic patients. We report here a case of endocarditis caused by C. ochracea. ...

journal_title：Journal of clinical microbiology

authors： Buu-Hoi AY,Joundy S,Acar JF

更新日期：1988-05-01 00:00:00

abstract：:The genetic diversity of 123 Streptococcus suis strains of capsular types 2, 1/2, 3, 7, and 9, isolated from pigs in France and from humans in different countries, was evaluated by pulsed-field gel electrophoresis (PFGE) of DNA restricted with SmaI. The method was highly discriminative (D = 0.98), results were reprodu...

journal_title：Journal of clinical microbiology

authors： Berthelot-Hérault F,Marois C,Gottschalk M,Kobisch M

更新日期：2002-02-01 00:00:00

abstract：:We conducted a molecular epidemiology study on 120 Mycobacterium tuberculosis isolates from patients presenting pulmonary tuberculosis (TB) in Burkina Faso. Classical antibiogram studies and genetic characterization, using mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) typing and ...

journal_title：Journal of clinical microbiology

authors： Godreuil S,Torrea G,Terru D,Chevenet F,Diagbouga S,Supply P,Van de Perre P,Carriere C,Bañuls AL

更新日期：2007-03-01 00:00:00

abstract：:First-void urine (FVU) is the preferred specimen for the diagnosis of urogenital Chlamydia trachomatis infection in men. We have developed FirstBurst, a urine collection device that collects the first 4 to 5 ml of FVU and yields a specimen with a sixfold higher C. trachomatis organism load than the regular urine cup b...

journal_title：Journal of clinical microbiology

authors： Wisniewski CA,White JA,Michel CE,Mahilum-Tapay L,Magbanua JP,Nadala EC Jr,Barber PJ,Goh BT,Lee HH

更新日期：2008-04-01 00:00:00

abstract：:Dried blood spot (DBS) specimens were assessed as an alternative to plasma for human immunodeficiency virus type 1 (HIV-1) serotyping by V3 loop peptide enzyme immunoassay. Nested PCR capable of distinguishing HIV-1 subtypes B and E was used as the reference standard. Ninety-two percent of DBS samples were typeable as...

journal_title：Journal of clinical microbiology

authors： Chanbancherd P,Brown AE,Trichavaroj R,Tienamporn P,Puthakird P,Limpairojn N,VanCott TC,de Souza MS

更新日期：1999-03-01 00:00:00

abstract：:Nontuberculous mycobacterial infections caused by Mycobacterium abscessus are responsible for a range of disease manifestations from pulmonary to skin infections and are notoriously difficult to treat, due to innate resistance to many antibiotics. Previous population studies of clinical M. abscessus isolates utilized ...

journal_title：Journal of clinical microbiology

authors： Davidson RM,Hasan NA,Reynolds PR,Totten S,Garcia B,Levin A,Ramamoorthy P,Heifets L,Daley CL,Strong M

更新日期：2014-10-01 00:00:00

abstract：:We document for the first time an infectious but nonarthritogenic variant of Borrelia burgdorferi. Strain 25015, previously isolated from an Ixodes dammini larva collected in upstate New York, was infectious but failed to produce arthritis or carditis in laboratory rats and mice. By contrast, pathogenic strain N40 inv...

journal_title：Journal of clinical microbiology

authors： Anderson JF,Barthold SW,Magnarelli LA

更新日期：1990-12-01 00:00:00

abstract：:The protease phenotypes expressed by isolates of Pseudomonas aeruginosa from cystic fibrosis (CF) patients were evaluated. The majority of isolates tested produced elastase (65%) or alkaline protease (64%) or both. The mucoid phenotype expressed by many CF isolates of P. aeruginosa did not absolutely restrict the expr...

journal_title：Journal of clinical microbiology

authors： Jagger KS,Bahner DR,Warren RL

更新日期：1983-01-01 00:00:00