Development of a Sensitive and Specific Serological Assay Based on Luminex Technology for Detection of Antibodies to Zaire Ebola Virus.

abstract：:Fimbriae of Porphyromonas gingivalis are filamentous appendages on the cell surface and are thought to be one of the virulence factors. The fimA gene encoding the subunit protein of fimbriae, fimbrillin (FimA), was classified into four typeable variants (types I to IV). We previously examined the distribution of P. gi...

journal_title：Journal of clinical microbiology

authors： Nakagawa I,Amano A,Kimura RK,Nakamura T,Kawabata S,Hamada S

更新日期：2000-05-01 00:00:00

abstract：:About 2.5 years after the first isolation of the VanA phenotype of vancomycin-resistant Enterococcus faecium (VREM) in Poland, the first VREM strains with the VanB phenotype have emerged independently in two different Warsaw hospitals. In one of these the VREM strain was selected during the long-term antimicrobial tre...

journal_title：Journal of clinical microbiology

authors： Kawalec M,Gniadkowski M,Zielińska U,Kłos W,Hryniewicz W

更新日期：2001-02-01 00:00:00

abstract：:CHROMagar Yersinia (CAY) is a new chromogenic medium for the presumptive detection of virulent Yersinia enterocolitica in stools. Based on a comparative analysis of 1,494 consecutive stools from hospitalized patients, CAY was found to be just as sensitive as the reference medium (cefsulodin-irgasan-novobiocin agar) bu...

journal_title：Journal of clinical microbiology

authors： Renaud N,Lecci L,Courcol RJ,Simonet M,Gaillot O

更新日期：2013-04-01 00:00:00

abstract：:Antibiotic neutralization in blood culture media from two automated systems was evaluated by measuring the recovery of organisms and times to detection in simulated cultures. Overall, BD Bactec Plus media (Bactec FX system) outperformed TREK 80 ml Redox media (VersaTREK system), although results suggest a relative rat...

journal_title：Journal of clinical microbiology

authors： Miller NS,Rogan D,Orr BL,Whitney D

更新日期：2011-04-01 00:00:00

abstract：:In a group of 596 delivering Ghanaian women, the sensitivities of peripheral blood thick film microscopy, ICT Malaria P.f/P.v test, and PCR in detecting microscopically confirmed placental Plasmodium falciparum infection were 42, 80, and 97%, respectively. In addition to the gross underestimation of placental malaria ...

journal_title：Journal of clinical microbiology

authors： Mockenhaupt FP,Ulmen U,von Gaertner C,Bedu-Addo G,Bienzle U

更新日期：2002-01-01 00:00:00

abstract：:Outbreaks of food-borne listeriosis have often involved strains of serotype 4b. Examination of multiple isolates from three different outbreaks revealed that ca. 11 to 29% of each epidemic population consisted of strains which were negative with the serotype-specific monoclonal antibody c74.22, lacked galactose from t...

journal_title：Journal of clinical microbiology

authors： Clark EE,Wesley I,Fiedler F,Promadej N,Kathariou S

更新日期：2000-10-01 00:00:00

abstract：:Recovery of aerobic and anaerobic bacteria from clinical specimens maintained in the Copan Venturi Transystem and the Becton Dickinson Port-a-Cul transport was assessed. Of 54 anaerobes, 53 were recovered after 4 h, and 52 were recovered after 24 h, from both systems. After 48 h, 45 and 50 were recovered from the two ...

journal_title：Journal of clinical microbiology

authors： Citron DM,Warren YA,Hudspeth MK,Goldstein EJ

更新日期：2000-02-01 00:00:00

abstract：:This report describes an unusual pathogen associated with post-traumatic meningitis. Haemophilus influenzae type D was isolated from both cerebrospinal fluid and blood of this patient with meningitis. ...

journal_title：Journal of clinical microbiology

authors： Viner JP,Massanari RM

更新日期：1979-02-01 00:00:00

abstract：:Development of resistance during therapy with cefamandole contributes to treatment failure. A simple cefoxitin disk test was recently described which detects a cefamandole-active inducible beta-lactamase not otherwise detectable with cefamandole as the inducer. A case of breakthrough Enterobacter bacteremia due to se...

journal_title：Journal of clinical microbiology

authors： Beckwith DG,Jahre JA

更新日期：1980-10-01 00:00:00

abstract：:The kinetics of delta antigen (HDAg) and anti-delta antibody (anti-HD) was analyzed in 22 acute delta hepatitis infections (11 coinfections and 11 superinfections), with an enzyme immunoassay developed by Organon Teknika and with two commercially available assays: Deltassay, a test for HDAg from Noctech and Abbott ant...

journal_title：Journal of clinical microbiology

authors： Dubois F,Goudeau A

更新日期：1988-07-01 00:00:00

abstract：:BHI agars supplemented with vancomycin 4 (BHI-V4) and 3 (BHI-V3) mg/liter have been proposed for screening vancomycin intermediately susceptible Staphylococcus aureus (VISA) and heteroresistant (hVISA) phenotypes, respectively, but growth interpretation criteria have not been established. We reviewed the growth result...

journal_title：Journal of clinical microbiology

authors： Khatib R,Riederer K,Sharma M,Shemes S,Iyer SP,Szpunar S

更新日期：2015-11-01 00:00:00

abstract：:Pseudobacteremias in blood cultures performed on the BACTEC radiometric blood culture system have been reported. We report three cases of cross contamination with Mycobacterium avium that occurred when mycobacteria were cultured with the BACTEC 460 TB system. Malfunction of the needle sterilization heating block was d...

journal_title：Journal of clinical microbiology

authors： Vannier AM,Tarrand JJ,Murray PR

更新日期：1988-09-01 00:00:00

abstract：:A 42-plex clustered regularly interspaced short palindromic repeat (CRISPR)-based typing technique (spoligotyping) was recently developed at the French National Reference Center for Legionella. It allows the subtyping of the Legionella pneumophila sequence type 1/Paris pulsotype. In this report, we present the transfe...

journal_title：Journal of clinical microbiology

authors： Gomgnimbou MK,Ginevra C,Peron-Cane C,Versapuech M,Refrégier G,Jacotin N,Sola C,Jarraud S

更新日期：2014-07-01 00:00:00

abstract：:A useful method for isolating and recognizing Haemophilus ducreyi from chancres and buboes of male patients is presented. A total of 41 clinical isolates of H. ducreyi were recovered from 33 patients over an 8-year period, and the experience with the 15 most recent isolates is presented in detail. Chocolate agar suppl...

journal_title：Journal of clinical microbiology

authors： Oberhofer TR,Back AE

更新日期：1982-04-01 00:00:00

abstract：:The peripheral blood lymphocytes from 48 heparinized blood specimens from human immunodeficiency virus (HIV) antibody-positive individuals were divided into two aliquots. One aliquot was reconstituted in one of the following five media. Medium 1 consisted of tryptose broth with 0.5% gelatin; medium 2 consisted of RPMI...

journal_title：Journal of clinical microbiology

authors： Gallo D,Kimpton JS,Johnson PJ

更新日期：1989-01-01 00:00:00

abstract：:The AutoMicrobic system (AMS) is capable of identifying most Enterobacteriaceae within 8 h and many glucose-nonfermenting, gram-negative bacilli after 13 h of incubation. Early preliminary results can be readily obtained from the computer as the tests incubate. Data with 1,023 bacterial isolates were reviewed to deter...

journal_title：Journal of clinical microbiology

authors： Barry AL,Badal RE

更新日期：1982-08-01 00:00:00

abstract：:Rotavirus is the most common cause of severe dehydrating gastroenteritis in infants. To date, 10 different serotypes of rotavirus have been identified in human stools. While four or five serotypes dominate, serotype circulation varies with season and geography. Since our laboratory has been involved in the development...

journal_title：Journal of clinical microbiology

authors： DiStefano DJ,Kraiouchkine N,Mallette L,Maliga M,Kulnis G,Keller PM,Clark HF,Shaw AR

更新日期：2005-12-01 00:00:00

abstract：:The semen plasma virus load is measured to ensure the safety of sperm processing during medically assisted procreation (MAP) for couples with a human immunodeficiency virus type 1 (HIV-1)-infected man. A practical, automated protocol using the COBAS Ampliprep CAP/CTM kit in the COBAS TaqMan96 system was developed to m...

journal_title：Journal of clinical microbiology

authors： Pasquier C,Sauné K,Raymond S,Moinard N,Daudin M,Bujan L,Izopet J

更新日期：2009-09-01 00:00:00

abstract：:The algorithm for a new identification system was designed on the basis of colony color and morphology on CHROMagar Orientation medium in conjunction with simple biochemical tests such as indole (IND), lysine decarboxylase (LDC), and ornithine decarboxylase (ODC) utilization tests with gram-negative bacilli isolated f...

journal_title：Journal of clinical microbiology

authors： Ohkusu K

更新日期：2000-12-01 00:00:00

abstract：:Nocardia identification required laborious and time-consuming phenotypic and chemotaxonomic methods until molecular methods were developed in the mid-1990s. Here we reassessed the capacity of PCR-restriction enzyme pattern analysis (PRA) of the hsp65 gene to differentiate Nocardia species, including 36 new species. Ou...

journal_title：Journal of clinical microbiology

authors： Rodríguez-Nava V,Couble A,Devulder G,Flandrois JP,Boiron P,Laurent F

更新日期：2006-02-01 00:00:00

abstract：:During an investigation of a hospital outbreak of norovirus gastroenteritis identified as being caused by a recombinant genogroup II (rGII-3a) strain, fecal specimens collected from asymptomatic staff and patients were tested by nested PCR. A GII-4 norovirus strain, the predominant strain associated with outbreaks in ...

journal_title：Journal of clinical microbiology

authors： Gallimore CI,Cubitt D,du Plessis N,Gray JJ

更新日期：2004-05-01 00:00:00

abstract：:Aeromonas caviae was recovered as the sole potential enteric pathogen from the stools of 14 of 17 symptomatic children (10 younger than 1 year of age) while Aeromonas hydrophila, Aeromonas sobria, and Plesiomonas shigelloides were isolated once each. The infants from whom A. caviae was isolated all presented with a wa...

journal_title：Journal of clinical microbiology

authors： Namdari H,Bottone EJ

更新日期：1990-05-01 00:00:00

abstract：:For testing bacterial susceptibility to PD131628, a 5-micrograms disk and the following tentative interpretive criteria may be used: > or = 19 mm for susceptible (MIC, < or = 1.0 micrograms/ml), 16 to 18 mm for intermediate (MIC, 2.0 micrograms/ml), and < or = 15 mm for resistant (MIC, > or = 4.0 micrograms/ml). For s...

journal_title：Journal of clinical microbiology

authors： Barry AL,Fuchs PC,Allen SD,Tenover FC,Jorgensen JH,Reller LB

更新日期：1995-01-01 00:00:00

abstract：:One of the greatest concerns in Chagas' disease is the absence of reliable methods for the evaluation of chemotherapy efficacy in treated patients. The tests available to evaluate cure after the specific treatment are the complement-mediated lysis (CoML) and flow cytometry tests, but they are not feasible for routine ...

journal_title：Journal of clinical microbiology

authors： Meira WS,Galvão LM,Gontijo ED,Machado-Coelho GL,Norris KA,Chiari E

更新日期：2004-02-01 00:00:00

abstract：:Accurate and timely diagnosis of dengue virus (DEN) infections is essential for the differential diagnosis of patients with febrile illness and hemorrhagic fever. In the present study, the diagnostic value of a newly developed immune-complex dissociated nonstructural-1 (NS-1) antigen dot blot immunoassay (DBI) was com...

journal_title：Journal of clinical microbiology

authors： Koraka P,Burghoorn-Maas CP,Falconar A,Setiati TE,Djamiatun K,Groen J,Osterhaus AD

更新日期：2003-09-01 00:00:00

abstract：:An automated RNA isolation procedure on the Qiagen BioRobot is described for performing viral load tests for human immunodeficiency virus (HIV) with the Amplicor HIV type 1 test. The new procedure improves the precision of the assay and requires significantly less labor than the presently used manual RNA isolation pro...

journal_title：Journal of clinical microbiology

authors： Xu M,Chan Y,Fischer SH,Remaley AT

更新日期：2004-01-01 00:00:00

abstract：:Preemptive ganciclovir (GCV) therapy is adopted increasingly in hematopoietic stem cell transplant (HCT) recipients, but occasional cases of increasing cytomegalovirus (CMV) antigenemia levels occur during preemptive GCV therapy. This prospective study investigated the incidence, risk factors, and clinical outcomes of...

journal_title：Journal of clinical microbiology

authors： Park SY,Lee SO,Choi SH,Kim YS,Woo JH,Sung H,Kim MN,Kim DY,Lee JH,Lee JH,Lee KH,Kim SH

更新日期：2011-12-01 00:00:00

abstract：:Immunoblots were used to study the immunoglobulin G response to Borrelia burgdorferi in experimentally and naturally exposed dogs. Adsorption studies confirmed that the antibodies were specific for B. burgdorferi. Experimentally exposed dogs were asymptomatic. Naturally exposed dogs included both asymptomatic animals ...

journal_title：Journal of clinical microbiology

authors： Greene RT,Walker RL,Nicholson WL,Heidner HW,Levine JF,Burgess EC,Wyand M,Breitschwerdt EB,Berkhoff HA

更新日期：1988-04-01 00:00:00

abstract：:Clostridium difficile is a major cause of antibiotic-associated gastrointestinal illness. Recently, an increased incidence of hospital-acquired infections with severe outcomes has been reported in North America and Europe. Current molecular-typing methods for detection of outbreaks and nosocomial transmission are labo...

journal_title：Journal of clinical microbiology

authors： Marsh JW,O'Leary MM,Shutt KA,Pasculle AW,Johnson S,Gerding DN,Muto CA,Harrison LH

更新日期：2006-07-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay was developed for the detection of circulating free and complexed staphylococcal antigens in various clinical categories of staphylococcal infections. Circulating immune complexes were studied by the polyethylene glycol precipitation method. Circulating immune complexes and staphyl...

journal_title：Journal of clinical microbiology

authors： Lentino JR,Rytel MW

更新日期：1982-12-01 00:00:00