Abstract:
:The semen plasma virus load is measured to ensure the safety of sperm processing during medically assisted procreation (MAP) for couples with a human immunodeficiency virus type 1 (HIV-1)-infected man. A practical, automated protocol using the COBAS Ampliprep CAP/CTM kit in the COBAS TaqMan96 system was developed to measure the HIV-1 load in semen plasma samples. HIV-1 was detected in 13.4% of the semen samples processed at our MAP center. Of the eight patients having a detectable semen HIV-1 load, five had no detectable virus in their blood plasma. This highlights the residual risk of HIV-1 transmission during unprotected intercourse and raises the question of the possible consequences of ineffective highly active antiretroviral therapy in the genital tract.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Pasquier C,Sauné K,Raymond S,Moinard N,Daudin M,Bujan L,Izopet Jdoi
10.1128/JCM.02131-08subject
Has Abstractpub_date
2009-09-01 00:00:00pages
2883-7issue
9eissn
0095-1137issn
1098-660Xpii
JCM.02131-08journal_volume
47pub_type
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.14.5.579-581.1981
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pub_type: 临床试验,杂志文章
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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