Abstract:
:The clinical value of immunoenzymatic (enzyme-linked immunosorbent assay) detection of anti-Toxoplasma immunoglobulin E (IgE) was assessed by studying 2,036 sera from 792 subjects, comprising seronegative controls and subjects with acute, active, reactivated, or congenital toxoplasmosis. Included were nonimmunized adults; pregnant women with recently acquired infection (acute toxoplasmosis); immunocompetent subjects with recently acquired severe infection (active toxoplasmosis) expressed as fever, adenopathies, splenomegaly, pneumonia, meningitis, or disseminated infection; subjects-some of them immunocompromised-whose previously moderate IgG antibody levels rose, suggesting a reactivation of quiescent toxoplasmosis; and infants born to seroconverted mothers and evaluated for diagnosis of congenital infection and therapeutic management. Specific IgE antibodies were never detected in seronegative subjects. They were present in 85.7% of asymptomatic seroconverters and in 100% of seroconverters with overt toxoplasmosis, following two different kinetics: in the former, the specific IgE titer generally presented a brief peak 2 to 3 months postinfection and then fell rapidly, whereas specific IgE persisted at a very high titer for several months in the latter. IgE emerged concomitantly with the increase in IgG during toxoplasmic reactivation. For neonatal diagnosis of congenital toxoplasmosis, IgE was less informative than IgM and IgA (sensitivities, 59.5, 64.3, and 76.2%, respectively) and had a specificity of 91.9%. Nevertheless, simultaneous measurement of the three isotypes at birth improved the diagnostic yield to 81% relative to the combination of IgA and IgM. Emergence of specific IgE during postnatal treatment for congenital toxoplasmosis is a sign of poor adherence or inadequate dosing.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Foudrinier F,Villena I,Jaussaud R,Aubert D,Chemla C,Martinot F,Pinon JMdoi
10.1128/jcm.41.4.1681-1686.2003subject
Has Abstractpub_date
2003-04-01 00:00:00pages
1681-6issue
4eissn
0095-1137issn
1098-660Xjournal_volume
41pub_type
杂志文章abstract::An evaluation was undertaken to determine the optimal method for testing the susceptibilities of 100 clinical isolates and two reference strains of Enterococcus spp. to vancomycin in vitro. Six testing methods were studied by using the following media and incubation times: agar screen with the Synergy Quad Plate (Reme...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.35.12.3258-3263.1997
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.27.12.2652-2655.1989
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.35.7.1813-1818.1997
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.25.1.158-159.1987
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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更新日期:2012-11-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.34.9.2231-2235.1996
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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更新日期:2009-09-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:
更新日期:1978-11-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.39.1.129-133.2001
更新日期:2001-01-01 00:00:00
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.36.6.1683-1687.1998
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journal_title:Journal of clinical microbiology
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.15.3.468-474.1982
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journal_title:Journal of clinical microbiology
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doi:10.1128/JCM.33.7.1807-1814.1995
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.37.12.3815-3821.1999
更新日期:1999-12-01 00:00:00
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journal_title:Journal of clinical microbiology
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更新日期:2010-10-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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更新日期:2003-01-01 00:00:00
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journal_title:Journal of clinical microbiology
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更新日期:1982-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:2012-01-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章,评审
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更新日期:2014-07-01 00:00:00
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journal_title:Journal of clinical microbiology
pub_type: 杂志文章
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更新日期:2001-04-01 00:00:00