Molecular typing of Yersinia pseudotuberculosis by using an IS200-like element.

abstract：:We evaluated a new rapid influenza diagnostic test for the pandemic (H1N1) 2009 influenza virus by using real-time reverse transcription-PCR (rRT-PCR) and viral culture. The sensitivities were 68.5% and 64.5%, and the specificities were 98.4% and 97.6%, respectively. This kit should be used with caution, and negative ...

journal_title：Journal of clinical microbiology

authors： Kwon D,Shin K,Kwon M,Oh HB,Kang C,Lee JY

更新日期：2011-01-01 00:00:00

abstract：:Bovine babesiosis is a serious threat to the cattle industry. We prepared blood DNA samples from 13 cattle with clinical babesiosis from the Badulla (n = 8), Jaffna (n = 3), and Kilinochchi (n = 2) districts in Sri Lanka. These DNA samples tested positive in PCR assays specific for Babesiabovis (n = 9), Babesia bigemi...

journal_title：Journal of clinical microbiology

authors： Sivakumar T,Tuvshintulga B,Zhyldyz A,Kothalawala H,Yapa PR,Kanagaratnam R,Vimalakumar SC,Abeysekera TS,Weerasingha AS,Yamagishi J,Igarashi I,Silva SSP,Yokoyama N

更新日期：2018-10-25 00:00:00

abstract：:Capnocytophaga ochracea is a gram-negative, fusiform bacillus which is part of the normal human oral flora. This organism is often isolated from periodontal lesions of patients with periodontitis and is associated with sepsis in granulocytopenic patients. We report here a case of endocarditis caused by C. ochracea. ...

journal_title：Journal of clinical microbiology

authors： Buu-Hoi AY,Joundy S,Acar JF

更新日期：1988-05-01 00:00:00

abstract：:The first World Health Organization (WHO) international standards (ISs) for nucleic acid amplification techniques were established two decades ago, with the initial focus on blood screening for three major viral targets, i.e., hepatitis C virus, hepatitis B virus, and human immunodeficiency virus 1. These reference ma...

journal_title：Journal of clinical microbiology

authors： Baylis SA,Wallace P,McCulloch E,Niesters HGM,Nübling CM

更新日期：2019-01-02 00:00:00

abstract：:This study used a nested multiplex PCR method to detect three periodontal pathogens in subgingival plaque collected before treatment and at 2 and 6 months posttreatment from 107 patients with severe, generalized periodontitis. The proportions of the patients who harbored these bacteria before periodontal treatment wer...

journal_title：Journal of clinical microbiology

authors： Ready D,D'Aiuto F,Spratt DA,Suvan J,Tonetti MS,Wilson M

更新日期：2008-10-01 00:00:00

abstract：:Four of eleven affected dogs died despite aggressive treatment during a 2015 focal outbreak of hemorrhagic gastroenteritis following a stay in a pet housing facility. Routine diagnostic investigations failed to identify a specific cause. Virus isolation from fresh necropsy tissues yielded a calicivirus with sequence h...

journal_title：Journal of clinical microbiology

authors： Renshaw RW,Griffing J,Weisman J,Crofton LM,Laverack MA,Poston RP,Duhamel GE,Dubovi EJ

更新日期：2018-04-25 00:00:00

abstract：:We described the molecular epidemiology of expanded-spectrum cephalosporin-resistant gram-negative bacilli (RGN) recovered from inanimate surfaces. RGN were isolated from 9% of environmental cultures. Numerous species, each with multiple unique strains, were recovered. Epidemiological links between environmental, pers...

journal_title：Journal of clinical microbiology

authors： D'Agata EM,Venkataraman L,DeGirolami P,Samore M

更新日期：1999-09-01 00:00:00

abstract：:Field surveys of malaria were performed in southern Vietnam by using an acridine orange staining method for rapid diagnosis and a PCR-based, microtiter plate hybridization method for accurate diagnosis. A total of three patients of Plasmodium ovale infection were detected, but PCR-amplified DNA of the P. ovale isolate...

journal_title：Journal of clinical microbiology

authors： Kawamoto F,Miyake H,Kaneko O,Kimura M,Nguyen TD,Nguyen TD,Liu Q,Zhou M,Le DD,Kawai S,Isomura S,Wataya Y

更新日期：1996-09-01 00:00:00

abstract：:Helicobacter pylori is known to be a major cause of peptic ulceration. The jhp0562 gene, encoding a glycosyltransferase involved in the synthesis of the lipopolysaccharide, was associated with peptic ulcer disease (PUD) in children. The beta-(1,3)-galactosyltransferase [beta-(1,3)GalT] gene (jhp0563), involved in Lewi...

journal_title：Journal of clinical microbiology

authors： Oleastro M,Santos A,Cordeiro R,Nunes B,Mégraud F,Ménard A

更新日期：2010-08-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay for the detection of Shiga toxins (Premier EHEC assay; Meridian Diagnostics, Inc.) was compared to conventional sorbitol-MacConkey culture for the recovery of enterohemorrhagic Escherichia coli. A total of 74 enteric pathogens, including 8 E. coli O157:H7 isolates, were recovered f...

journal_title：Journal of clinical microbiology

authors： Kehl KS,Havens P,Behnke CE,Acheson DW

更新日期：1997-08-01 00:00:00

abstract：:A bacitracin (0.04 units, BBL)-SXT (trimethoprim, 1.25 mg, plus sulfamethoxazole, 23.75 mg, BBL) susceptibility test was 94% accurate in presumptively identifying streptococci as either group A, B or not group A and B. ...

journal_title：Journal of clinical microbiology

authors： Gunn BA

更新日期：1976-08-01 00:00:00

abstract：:A rapid dimethyl sulfoxide modification of an acid-fast technique was applied to direct fecal smears to monitor cryptosporidiosis in nonhuman primates. Brilliantly stained pink oocysts against a pale green background demonstrated well-preserved internal morphology and facilitated rapid, simple, noninvasive diagnosis w...

journal_title：Journal of clinical microbiology

authors： Bronsdon MA

更新日期：1984-06-01 00:00:00

abstract：:The etiologies of a number of retinopathies, including serpiginous choroiditis and acute zonal occult outer retinopathy (AZOOR), remain uncertain. Recently, we provided evidence that AZOOR is caused by Candida famata infection. The purpose of this article was to investigate the presence of fungal infection in five pat...

journal_title：Journal of clinical microbiology

authors： Pisa D,Ramos M,García P,Escoto R,Barraquer R,Molina S,Carrasco L

更新日期：2008-01-01 00:00:00

abstract：:Although it has been recommended that photochromogenicity of mycobacteria should be tested after incubation at 25 to 30 degrees C for 2 weeks (Wayne et al., Int. J. Syst. Bacteriol. 24:412-419, 1974), our results indicate that photochromogenicity should be determined at 37 degrees C in the dark and under continuous ex...

journal_title：Journal of clinical microbiology

authors： Tsukamura M

更新日期：1981-08-01 00:00:00

abstract：:During pregnancy, Plasmodium falciparum infection of the placenta frequently occurs in the absence of parasites in peripheral blood. We investigated the abilities of the OptiMAL rapid immunochromatographic strip test for P. falciparum lactate dehydrogenase and species-specific PCR performed on peripheral blood to dete...

journal_title：Journal of clinical microbiology

authors： Mankhambo L,Kanjala M,Rudman S,Lema VM,Rogerson SJ

更新日期：2002-01-01 00:00:00

abstract：:Between January 1988 and December 1989 Aeromonas species were isolated from 45 (1.8%) of 2,480 patients with acute gastroenteritis. No other bacterial enteric pathogens were found in any of these 45 patients. Of the 45 Aeromonas isolates, 35 strains (77.8%) were Aeromonas hydrophila, 7 (15.5%) were Aeromonas sobria, a...

journal_title：Journal of clinical microbiology

authors： Deodhar LP,Saraswathi K,Varudkar A

更新日期：1991-05-01 00:00:00

abstract：:Many truly human immunodeficiency virus (HIV) antibody-negative serum samples may be unnecessarily subjected to costly and time-consuming Western blots (immunoblots). An investigation was undertaken to evaluate the efficiency of using a recombinant protein-based enzyme immunosorbent assay (EIA; Cambridge BioScience [C...

journal_title：Journal of clinical microbiology

authors： Lepine DG,Neumann PW,Frenette SL,O'Shaughnessy MV

更新日期：1990-06-01 00:00:00

abstract：:A diagnostic test for feline infectious peritonitis virus (FIPV) infection based on a nested PCR (nPCR) assay was developed and tested with FIPV, feline enteric coronavirus (FECV), canine coronavirus (CCV), and transmissible gastroenteritis virus (TGEV) and clinical fluid samples from cats with effusive feline infecti...

journal_title：Journal of clinical microbiology

authors： Gamble DA,Lobbiani A,Gramegna M,Moore LE,Colucci G

更新日期：1997-03-01 00:00:00

abstract：:A reversed passive latex agglutination (RPLA) assay for determining the toxigenicity of Corynebacterium diphtheriae is presented. Rabbit antitoxin antiserum was raised by using commercially available diphtheria toxoid. This antiserum reacted with the diphtheria toxin when the culture supernatant was assayed by Western...

journal_title：Journal of clinical microbiology

authors： Toma C,Sisavath L,Iwanaga M

更新日期：1997-12-01 00:00:00

abstract：:A simple solid-phase enzyme-linked immunosorbent assay procedure for the detection of human antibodies to Entamoeba histolytica was developed which showed a high degree of correlation with the agar gel diffusion, counterelectrophoresis, and indirect hemagglutination methods, as well as with clinical data. The enzyme-l...

journal_title：Journal of clinical microbiology

authors： Lin TM,Halbert SP,Chiu CT,Zarco R

更新日期：1981-04-01 00:00:00

abstract：:Enteroaggregative Escherichia coli (EAEC) is an emerging enteric pathogen that causes acute and chronic diarrhea among children, human immunodeficiency virus-infected patients, and travelers to developing regions of the world. The pathogenesis of EAEC strains involves the production of biofilm. In this study, we deter...

journal_title：Journal of clinical microbiology

authors： Mohamed JA,Huang DB,Jiang ZD,DuPont HL,Nataro JP,Belkind-Gerson J,Okhuysen PC

更新日期：2007-01-01 00:00:00

abstract：:A singleplex PCR assay using a single primer pair targeting the putative sugar transporter gene was developed here to distinguish Cryptococcus neoformans var. grubii, Cryptococcus neoformans var. neoformans, and Cryptococcus gattii according to the distinct size of the amplicon. The interspecies and intravarietal hybr...

journal_title：Journal of clinical microbiology

authors： Feng X,Fu X,Ling B,Wang L,Liao W,Yao Z

更新日期：2013-06-01 00:00:00

abstract：:We investigated a method to reduce oxygen tension and produce anaerobic conditions conveniently by coculture with a nonfermentative organism. The biochemical reactions of 94 strains representing 41 different species were determined using prereduced media and gas chromatography according to standard methods. The standa...

journal_title：Journal of clinical microbiology

authors： Hussain Z,Bürger H,Lannigan R,Groves DJ

更新日期：1984-02-01 00:00:00

abstract：:Sequence-specific PCR was used in six laboratories and a ligase detection reaction was used in one laboratory to detect the zidovudine-resistance mutation at codon 215 of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase DNA. The genotypes of 27 different clinical samples, including cultured HIV-1 isol...

journal_title：Journal of clinical microbiology

authors： Shafer RW,Winters MA,Mayers DL,Japour AJ,Kuritzkes DR,Weislow OS,White F,Erice A,Sannerud KJ,Iversen A,Pena F,Dimitrov D,Frenkel LM,Reichelderfer PS

更新日期：1996-07-01 00:00:00

abstract：:Recently, three novel avian-origin swine influenza viruses (SIVs) were first isolated from pigs in Guangdong Province, southern China, yet little is known about the seroprevalence of avian influenza viruses among pigs in southern China. Here, we report for the first time the seroprevalence of avian H3, H4, and H6 infl...

journal_title：Journal of clinical microbiology

authors： Su S,Qi W,Chen J,Zhu W,Huang Z,Xie J,Zhang G

更新日期：2013-02-01 00:00:00

abstract：:By two PCR-based diagnostic methods, Plasmodium malariae infections have been rediscovered at two foci in the Sichuan province of China, a region where no cases of P. malariae have been officially reported for the last 2 decades. In addition, a variant form of P. malariae which has a deletion of 19 bp and seven substi...

journal_title：Journal of clinical microbiology

authors： Liu Q,Zhu S,Mizuno S,Kimura M,Liu P,Isomura S,Wang X,Kawamoto F

更新日期：1998-11-01 00:00:00

abstract：:A simple agar-well diffusion bioassay suitable for measurement of flucytosine or ketoconazole was developed by using Candida pseudotropicalis ATCC 46764 as the assay organism. A test medium composed of (per liter) 7 g of Trypticase peptone, 7 g of YNB (yeast-nitrogen base), 15 g of glucose, and 15 g of agar was seeded...

journal_title：Journal of clinical microbiology

authors： Bodet CA 3rd,Jorgensen JH,Drutz DJ

更新日期：1985-08-01 00:00:00

abstract：:This three-center collaborative study was conducted to evaluate samples of the Microbial Profile System (MPS) antimicrobial microdilution panels [previously produced by Minnesota Mining & Manufacturing Co., (3M Co.), St. Paul, Minn. and currently produced by Flow Laboratories, Inc., Rockville, Md.). This was a three-p...

journal_title：Journal of clinical microbiology

authors： Gerlach EH,Jones RN,Barry AL

更新日期：1983-03-01 00:00:00

abstract：:Immunoglobulin M (IgM) and IgA antibodies against the major surface protein of Toxoplasma gondii were determined in a total of 195 human sera and five human cerebrospinal fluids by using a P30 membrane extract and the immunoblot technique. By using two different T. gondii strains (RH and BK) simultaneously as antigens...

journal_title：Journal of clinical microbiology

authors： Gross U,Roos T,Appoldt D,Heesemann J

更新日期：1992-06-01 00:00:00

abstract：:Ten cultivable equine rotavirus isolates, two of North American, six of British, and two of Irish origin, were compared with standard rotavirus strains and with each other by cross neutralization, neutralization with a panel of monoclonal antibodies (MAbs), hybridization to a simian rotavirus (SA-11) VP7 gene probe, a...

journal_title：Journal of clinical microbiology

authors： Browning GF,Chalmers RM,Fitzgerald TA,Snodgrass DR

更新日期：1992-02-01 00:00:00