Development and evaluation of a rapid influenza diagnostic test for the pandemic (H1N1) 2009 influenza virus.

abstract：:This study examined the genetic diversity and dynamicity of circulating Mycobacterium tuberculosis strains in Thailand using nearly neutral molecular markers. The single nucleotide polymorphism (SNP)-based genotypes of 1,414 culture-positive M. tuberculosis isolates from 1,282 pulmonary tuberculosis (PTB) and 132 extr...

journal_title：Journal of clinical microbiology

authors： Srilohasin P,Chaiprasert A,Tokunaga K,Nishida N,Prammananan T,Smittipat N,Mahasirimongkol S,Chaiyasirinroje B,Yanai H,Palittapongarnpim P

更新日期：2014-12-01 00:00:00

abstract：:Forested areas adjacent to Milwaukee, Wis., and Chicago, Ill., were investigated for rodents and ticks infected with Borrelia burgdorferi, the causative agent of Lyme disease. White-footed mice (Peromyscus leucopus or Peromyscus maniculatus), meadow voles (Microtus pennsylvanicus), and eastern chipmunks (Tamias striat...

journal_title：Journal of clinical microbiology

authors： Callister SM,Nelson JA,Schell RF,Jobe DA,Bautz R,Agger WA,Coggins J

更新日期：1991-02-01 00:00:00

abstract：:We compared the BD MAX enteric bacterial panel (EBP) to culture for the detection of Salmonella, Shigella, Campylobacter, and Shiga toxin-producing enterohemorrhagic Escherichia coli (EHEC) O157 in seeded stool samples. The EBP panel demonstrated superior sensitivity and reliably detected Salmonella, EHEC O157, Shigel...

journal_title：Journal of clinical microbiology

authors： Anderson NW,Buchan BW,Ledeboer NA

更新日期：2014-04-01 00:00:00

abstract：:Whole-cell chromosomal digests of 54 isolates of Streptococcus suis encompassing all known serotypes from a geographically varied collection were examined by PstI restriction fragment length polymorphisms and then hybridized with a digoxigenin-11-dUTP-labeled cDNA probe transcribed from a mixture of 16S and 23S rRNAs ...

journal_title：Journal of clinical microbiology

authors： Okwumabua O,Staats J,Chengappa MM

更新日期：1995-04-01 00:00:00

abstract：:Methicillin-resistant Staphylococcus aureus (MRSA) of sequence type 398 (ST398) has frequently been detected in pigs and pig handlers. However, in Malaysia, sampling 360 pigs and 90 pig handlers from 30 farms identified novel ST9-spa type t4358-staphylococcal cassette chromosome mec type V MRSA strains that were found...

journal_title：Journal of clinical microbiology

authors： Neela V,Mohd Zafrul A,Mariana NS,van Belkum A,Liew YK,Rad EG

更新日期：2009-12-01 00:00:00

abstract：:Blood culture records from 1994 to 1995 from five U.S. medical centers all using the Difco ESP continuous monitoring blood culture system were reviewed retrospectively. Among a total of 7,362 isolates of bacteria and yeasts, only 0.1% of possibly significant isolates would have been missed had blood cultures been rout...

journal_title：Journal of clinical microbiology

authors： Doern GV,Brueggemann AB,Dunne WM,Jenkins SG,Halstead DC,McLaughlin JC

更新日期：1997-05-01 00:00:00

abstract：:A previously described sequence-based epidemiological typing method for clinical and environmental isolates of Legionella pneumophila serogroup 1 was extended by the investigation of three additional gene targets and modification of one of the previous targets. Excellent typeability, reproducibility, and epidemiologic...

journal_title：Journal of clinical microbiology

authors： Gaia V,Fry NK,Afshar B,Lück PC,Meugnier H,Etienne J,Peduzzi R,Harrison TG

更新日期：2005-05-01 00:00:00

abstract：:A total of 122 adenovirus type 4 (Ad4) strains isolated from patients with epidemic keratoconjunctivitis at an eye clinic in Sapporo, Japan, from 1985 to 1989 were identified as Ad4a. By the use of HinfI and TaqI restriction endonucleases, these Ad4a strains were classified into 12 subgenome types. During this observa...

journal_title：Journal of clinical microbiology

authors： Itakura S,Aoki K,Sawada H,Ishiguro N,Shinagawa M

更新日期：1991-08-01 00:00:00

abstract：:Rabies virus found worldwide and prevalent throughout the United States continues to be a public health concern. Direct-fluorescent antibody (DFA) detection remains the gold standard for rabies virus diagnostics. Assessing the utility of a high-throughput molecular platform such as the QIAsymphony SP/AS, in conjunctio...

journal_title：Journal of clinical microbiology

authors： Dupuis M,Brunt S,Appler K,Davis A,Rudd R

更新日期：2015-09-01 00:00:00

abstract：:Several patients receiving blood transfusions during the summer of 1991 developed bacteremia after the transfusion. In all cases, the infection was caused by Serratia marcescens. The same strain of Serratia marcescens was isolated from the patients and from the outer surface of unfilled blood bags. The transport conta...

journal_title：Journal of clinical microbiology

authors： Szewzyk U,Szewzyk R,Stenström TA

更新日期：1993-07-01 00:00:00

abstract：:The new commercial assay GenoType Mycobacterium, intended for the identification of mycobacteria, was evaluated with a panel of 197 strains belonging to 86 different taxa. The system, which relies on solid-phase reverse hybridization, is composed of two different kits: GenoType CM for the identification of more freque...

journal_title：Journal of clinical microbiology

authors： Russo C,Tortoli E,Menichella D

更新日期：2006-02-01 00:00:00

abstract：:Panton-Valentine leukocidin (PVL) is associated with staphylococcal skin and pulmonary infections. Community dissemination of PVL-producing Staphylococcus aureus strains constitutes a public health concern. Family transmission and spread of community-acquired leukocidin-positive methicillin-susceptible S. aureus ST152...

journal_title：Journal of clinical microbiology

authors： Pérez-Roth E,Alcoba-Flórez J,López-Aguilar C,Gutiérrez-González I,Rivero-Pérez B,Méndez-Alvarez S

更新日期：2010-01-01 00:00:00

abstract：:A disease similar to ulcerative colitis in humans has been identified in cotton-top tamarins (CTTs) in captivity. The clinical signs include weight loss, diarrhea, and rectal bleeding with the pathological features and biochemical abnormalities of ulcerative colitis. Approximately 25 to 40% of these animals develop co...

journal_title：Journal of clinical microbiology

authors： Saunders KE,Shen Z,Dewhirst FE,Paster BJ,Dangler CA,Fox JG

更新日期：1999-01-01 00:00:00

abstract：:At the time indwelling urinary catheters were removed, the urethral flora was sampled by rolling the catheters onto culture plates. Cultures were obtained from 398 patients with sterile urine. Catheter surfaces were colonized less often in males than in females (16.8 and 67.0%, respectively) and yielded correspondingl...

journal_title：Journal of clinical microbiology

authors： Kunin CM,Steele C

更新日期：1985-06-01 00:00:00

abstract：:Potential causes of congenital infection include Toxoplasma gondii and viruses such as cytomegalovirus (CMV), enterovirus, hepatitis C virus, herpes simplex virus types 1 and 2 (HSV-1 and -2), human herpesvirus types 6, 7, and 8, lymphocytic choriomeningitis virus, parvovirus, rubella virus, and varicella-zoster virus...

journal_title：Journal of clinical microbiology

authors： McIver CJ,Jacques CF,Chow SS,Munro SC,Scott GM,Roberts JA,Craig ME,Rawlinson WD

更新日期：2005-10-01 00:00:00

abstract：:Due to the inadequate automation in the amplification and sequencing procedures, the use of 16S rRNA gene sequence-based methods in clinical microbiology laboratories is largely limited to identification of strains that are difficult to identify by phenotypic methods. In this study, using conventional full-sequence 16...

journal_title：Journal of clinical microbiology

authors： Woo PC,Ng KH,Lau SK,Yip KT,Fung AM,Leung KW,Tam DM,Que TL,Yuen KY

更新日期：2003-05-01 00:00:00

abstract：:Given the rise in the incidence of invasive fungal infections (IFIs) and the expanding spectrum of fungal pathogens, early and accurate identification of the causative pathogen is essential. We developed a panfungal PCR assay that targets the internal transcribed spacer 1 (ITS1) region of the ribosomal DNA gene cluste...

journal_title：Journal of clinical microbiology

authors： Lau A,Chen S,Sorrell T,Carter D,Malik R,Martin P,Halliday C

更新日期：2007-02-01 00:00:00

abstract：:Inconsistent quality control results in disk diffusion testing of cefaclor, cefamandole, cefonicid, and cefuroxime with Haemophilus influenzae ATCC 49247 and Haemophilus test medium (HTM) prompted a search for an alternative control strain that would provide more reliable results. A five-laboratory study was conducted...

journal_title：Journal of clinical microbiology

authors： Jorgensen JH,Barry AL,Doern GV,Ferraro MJ,Murray PR

更新日期：1992-08-01 00:00:00

abstract：:A typing enzyme immunoassay (TYPE-EIA) was used to determine the antigenic types of 64 astrovirus-positive specimens from nine collections from seven countries. Six of the seven known astrovirus types were detected in the collections, with HAstV-1 predominating in all collections for one from the United Kingdom. Selec...

journal_title：Journal of clinical microbiology

authors： Noel JS,Lee TW,Kurtz JB,Glass RI,Monroe SS

更新日期：1995-04-01 00:00:00

abstract：:A case of tick-borne encephalitis (TBE) has not been reported for many years in Japan, although a serological survey of sera from domestic animals suggested the presence of TBE foci in Hokkaido, the northern island of Japan. Studies were conducted to prove the presence of an endemic focus of TBE virus in Japan by mean...

journal_title：Journal of clinical microbiology

authors： Takashima I,Morita K,Chiba M,Hayasaka D,Sato T,Takezawa C,Igarashi A,Kariwa H,Yoshimatsu K,Arikawa J,Hashimoto N

更新日期：1997-08-01 00:00:00

abstract：:The EasyNAT assay was evaluated for the detection of tuberculosis in sputum smears from presumptive pulmonary tuberculosis (TB) patients in an African high-TB and high-HIV setting. The sensitivity of the EasyNAT assay was 66.7%, and the specificity and positive predictive value were 100% for the culture-positive patie...

journal_title：Journal of clinical microbiology

authors： Mhimbira FA,Bholla M,Sasamalo M,Mukurasi W,Hella JJ,Jugheli L,Reither K

更新日期：2015-04-01 00:00:00

abstract：:A rapid and simple method for extracting specific cell-free antigens of Coccidioides immitis and Histoplasma capsulatum from agar slant cultures was developed. The extracts were analyzed in immunodiffusion tests for the presence of C. immitis or H. capsulatum specific antigens. These extracts were compared with cultur...

journal_title：Journal of clinical microbiology

authors： Kaufman L,Standard P

更新日期：1978-07-01 00:00:00

abstract：:The efficacy of antiviral treatment for chronic hepatitis C virus (HCV) infection is determined by measuring HCV RNA at specific time points throughout therapy using highly sensitive and accurate HCV RNA assays. This study compared the performances of two recently developed real-time PCR HCV RNA assays, cobas HCV for ...

journal_title：Journal of clinical microbiology

authors： Vermehren J,Stelzl E,Maasoumy B,Michel-Treil V,Berkowski C,Marins EG,Paxinos EE,Marino E,Wedemeyer H,Sarrazin C,Kessler HH

更新日期：2017-04-01 00:00:00

abstract：:We previously demonstrated the transmission of murine cytomegalovirus to syngeneic mice and preformed monolayers of mouse embryo fibroblasts with plastic-adherent peritoneal exudate cells from infected mice as a source of macrophages. In the present studies we compared this standard feeder layer method with a reverse ...

journal_title：Journal of clinical microbiology

authors： Winkler SR,Booss J

更新日期：1980-12-01 00:00:00

abstract：:In a study of 1,486 men attending two sexually transmitted disease clinics, of whom 891 had no symptoms of urethritis, we compared an enzyme immunoassay (EIA) (Baxter-Bartels, formerly Northumbria AntigEnz) of urine sediment to urethral culture for the detection of Chlamydia trachomatis. C. trachomatis prevalence by c...

journal_title：Journal of clinical microbiology

authors： Sanders JW,Hook EW 3rd,Welsh LE,Shepherd ME,Quinn TC

更新日期：1994-01-01 00:00:00

abstract：:Since the use of highly purified viral proteins in the solid-phase radioimmunoassay (SPRIA) procedure is not practical for routine diagnostic serology, the procedure was adapted for use with the crude antigens now commonly used to diagnose St. Louis encephalitis (SLE) virus infections. SLE and various other alpha- and...

journal_title：Journal of clinical microbiology

authors： Wolff KL,Muth DJ,Hudson BW,Trent DW

更新日期：1981-08-01 00:00:00

abstract：:Plasmid-mediated colistin resistance (PMCR) is a global public health concern, given its ease of transmissibility. The purpose of this study was to evaluate two methods for the detection of PMCR from bacterial colonies: (i) the NG-Test MCR-1 lateral flow immunoassay (LFA; NG Biotech, Guipry, France) and (ii) the EDTA-...

journal_title：Journal of clinical microbiology

authors： Fenwick AJ,Bergman Y,Lewis S,Yee R,Uhlemann AC,Cole N,Kohner P,Ordak C,Green DA,Schuetz AN,Humphries R,Simner PJ

更新日期：2020-03-25 00:00:00

abstract：:Recent outbreaks of severe acute respiratory syndrome (SARS) have spurred intense research efforts around the world to deal with the serious threat to health posed by this novel coronavirus. A rapid, reliable diagnostic assay is needed for monitoring the spread of the disease. Here we report a method for eliminating f...

journal_title：Journal of clinical microbiology

authors： Hui RK,Zeng F,Chan CM,Yuen KY,Peiris JS,Leung FC

更新日期：2004-05-01 00:00:00

abstract：:An enzyme immunoassay (EIA) for detection of immunoglobulin M (IgM) and IgG antibodies to Mycoplasma pneumoniae was developed. The EIA was evaluated on the basis of results in the M. pneumoniae complement fixation (MPCF) test and the cold agglutinin test. Serum samples from 430 patients with respiratory infections of ...

journal_title：Journal of clinical microbiology

authors： Uldum SA,Jensen JS,Søndergård-Andersen J,Lind K

更新日期：1992-05-01 00:00:00

abstract：:A strain of Escherichia coli isolated from a child with diarrhea was biochemically similar and antigenically identical (O and K antigens) to the standard strain of E. coli O group 29 and was positive in the Serény test for invasiveness, which suggests that it can cause a Shigella-like illness. ...

journal_title：Journal of clinical microbiology

authors： Toledo MR,Reis MH,Almeida RG,Trabulsi LR

更新日期：1979-02-01 00:00:00