Evaluation of a human immunodeficiency virus test algorithm utilizing a recombinant protein enzyme immunoassay.

abstract：:Burkholderia pseudomallei is the causative agent of melioidosis. In northeast Thailand, this gram-negative bacterium is a major cause of mortality from septicemia. The definitive diagnosis of this disease is made by bacterial culture. In this study, we produced a monoclonal antibody (MAb) specific to the 30-kDa protei...

journal_title：Journal of clinical microbiology

authors： Pongsunk S,Thirawattanasuk N,Piyasangthong N,Ekpo P

更新日期：1999-11-01 00:00:00

abstract：:The QuantiFERON-TB Gold Plus (QFT-Plus; Qiagen, Germantown, MD) interferon gamma release assay (IGRA) received FDA clearance in 2017 and will replace the prior version of the assay, the QFT-Gold In-Tube (QFT-GIT). Here, we compared performances of the QFT-Plus assay and the QFT-GIT version in a diverse patient populat...

journal_title：Journal of clinical microbiology

authors： Theel ES,Hilgart H,Breen-Lyles M,McCoy K,Flury R,Breeher LE,Wilson J,Sia IG,Whitaker JA,Clain J,Aksamit TR,Escalante P

更新日期：2018-06-25 00:00:00

abstract：:In a long-term rodent bioassay evaluating the carcinogenicity of triethanolamine, there was equivocal evidence of carcinogenic activity in male B6C3F1 mice, based on a marginal increase in the number of hepatocellular adenomas and hepatoblastomas. Interpretation was complicated by the presence of Helicobacter hepaticu...

journal_title：Journal of clinical microbiology

authors： Fox JG,MacGregor JA,Shen Z,Li X,Lewis R,Dangler CA

更新日期：1998-05-01 00:00:00

abstract：:Fungal infection of the thyroid is rare. Most reported cases have involved Aspergillus, Coccidioides, and Candida species in the setting of disseminated disease. Infection of the thyroid with Histoplasma capsulatum is rarely reported as part of disseminated disease, even in geographic areas where histoplasmosis is end...

journal_title：Journal of clinical microbiology

authors： Goldani LZ,Klock C,Diehl A,Monteiro AC,Maia AL

更新日期：2000-10-01 00:00:00

abstract：:We determined the quantitative levels and the genetic mechanisms of resistance in drug-resistant clinical isolates of Mycobacterium tuberculosis sampled over a period of 3 years (n = 45; 17 of the isolate were multidrug resistant). Our results led us to hypothesize that some strains categorized as resistant to isoniaz...

journal_title：Journal of clinical microbiology

authors： Springer B,Calligaris-Maibach RC,Ritter C,Böttger EC

更新日期：2008-12-01 00:00:00

abstract：:Four strains of Campylobacter jejuni and four strains of Campylobacter coli were used to compare the quantitative growth of Campylobacter cells on blood agar base no. 2 (Oxoid), brucella agar (BBL Microbiology Systems and Difco Laboratories), campylobacter agar base (Difco), Columbia blood agar base (Difco and Oxoid),...

journal_title：Journal of clinical microbiology

authors： Ng LK,Stiles ME,Taylor DE

更新日期：1985-02-01 00:00:00

abstract：:We developed a group B streptococcus multiplex PCR assay which allows, by direct analysis of the amplicon size, determination of the surface protein antigen genes of alpha-C protein, epsilon protein, Rib, Alp2, Alp3, and Alp4. The multiplex PCR assay offers a rapid and simple method of subtyping Streptococcus agalacti...

journal_title：Journal of clinical microbiology

authors： Creti R,Fabretti F,Orefici G,von Hunolstein C

更新日期：2004-03-01 00:00:00

abstract：:Multiple-locus variable-number tandem-repeat analysis (MLVA) was performed with 292 methicillin-resistant Staphylococcus aureus (MRSA) isolates previously characterized by pulsed-field gel electrophoresis, multilocus sequence typing, and staphylococcal cassette chromosome mec typing. Quantitative correspondence analys...

journal_title：Journal of clinical microbiology

authors： Rivero-Pérez B,Pérez-Roth E,Méndez-Alvarez S

更新日期：2010-08-01 00:00:00

abstract：:The rapid identification of blood culture isolates and antimicrobial susceptibility test (AST) results play critical roles for the optimal treatment of patients with bloodstream infections. Whereas others have looked at the time to detection in automated culture systems, we examined the overall time from specimen coll...

journal_title：Journal of clinical microbiology

authors： Tabak YP,Vankeepuram L,Ye G,Jeffers K,Gupta V,Murray PR

更新日期：2018-11-27 00:00:00

abstract：:A study of 180 healthy cats found that 15.6% were feline leukemia virus (FeLV) positive, 8.3% were feline immunodeficiency virus (FIV) positive, and 1.1% were FIV and FeLV positive, which corresponded to 30.4, 13.8, and 2.6, of 115 cats with FIV- and FeLV-related symptoms, respectively. Differences were seen in the se...

journal_title：Journal of clinical microbiology

authors： Arjona A,Escolar E,Soto I,Barquero N,Martin D,Gomez-Lucia E

更新日期：2000-09-01 00:00:00

abstract：:Leuconostoc species are emerging pathogens that can cause severe infections, particularly in immunocompromised patients. Using molecular methods, we identified Leuconostoc mesenteroides as the cause of a brain abscess which was successfully treated by surgery and antimicrobial treatment. This is the first report of br...

journal_title：Journal of clinical microbiology

authors： Albanese A,Spanu T,Sali M,Novegno F,D'Inzeo T,Santangelo R,Mangiola A,Anile C,Fadda G

更新日期：2006-08-01 00:00:00

abstract：:We describe the serum immunoglobulin A (IgA) antibody response to Norwalk virus infection in human volunteers and compare it with previously described IgM and total antibody responses. Whereas specific IgA and IgM peak within 2 weeks after onset of symptoms, titers of total blocking antibody continue to rise, implying...

journal_title：Journal of clinical microbiology

authors： Erdman DD,Gary GW,Anderson LJ

更新日期：1989-06-01 00:00:00

abstract：:Cerebrospinal fluid samples from 257 patients with suspected herpes simplex virus encephalitis were prospectively analyzed by herpes simplex virus polymerase chain reaction. The polymerase chain reaction indicated herpes simplex virus encephalitis in 9 serologically proven cases and in 14 additional patients. Increase...

journal_title：Journal of clinical microbiology

authors： Puchhammer-Stöckl E,Heinz FX,Kundi M,Popow-Kraupp T,Grimm G,Millner MM,Kunz C

更新日期：1993-01-01 00:00:00

abstract：:Detection of group B streptococcus (GBS) antigen in urine by latex particle agglutination (LPA) may facilitate the rapid diagnosis of GBS sepsis. We sought to compare three commercial LPA assays with specimens that were spiked with type-specific antigen, group-specific antigen, or type III organisms. There were sensit...

journal_title：Journal of clinical microbiology

authors： Ascher DP,Wilson S,Fischer GW

更新日期：1991-12-01 00:00:00

abstract：:We evaluated the performance and the cost of toxigenic culture using a commercial chromogenic medium (CDIF) for 538 stool specimens. Compared with real-time PCR, this method was found to detect an additional 9% of positive specimens and result in 61% reduction in material costs, with a trade-off increase in turnaround...

journal_title：Journal of clinical microbiology

authors： Luk S,To WK,Ng TK,Hui WT,Lee WK,Lau F,Ching AM

更新日期：2014-02-01 00:00:00

abstract：:The emerging multidrug-resistant pathogenic yeast Candida auris represents a serious threat to global health. Unlike most other Candida species, this organism appears to be commonly transmitted within health care facilities and causes health care-associated outbreaks. To better understand the epidemiology of this emer...

journal_title：Journal of clinical microbiology

authors： Welsh RM,Bentz ML,Shams A,Houston H,Lyons A,Rose LJ,Litvintseva AP

更新日期：2017-10-01 00:00:00

abstract：:A total of 50 strains of Staphylococcus aureus, including 41 methicillin-resistant S. aureus (MRSA) strains, were characterized by one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins. The protein patterns contained 40-50 discrete bands and were highly reproducible. Partial ...

journal_title：Journal of clinical microbiology

authors： Costas M,Cookson BD,Talsania HG,Owen RJ

更新日期：1989-11-01 00:00:00

abstract：:A loop-mediated isothermal amplification (LAMP) method for the rapid detection of serotype 1 Marek's disease virus (MDV) was developed. The method used a set of three pairs of primers to amplify the MEQ gene for detecting serotype 1 MDV. The MDV LAMP method did not cross-react with serotype 2 and serotype 3, nor did t...

journal_title：Journal of clinical microbiology

authors： Angamuthu R,Baskaran S,Gopal DR,Devarajan J,Kathaperumal K

更新日期：2012-03-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay was used to measure nasal-wash and serum isotype-specific hemagglutinin antibody responses in 109 seronegative (hemagglutination-inhibiting titer less than or equal to 1:8) adults vaccinated intranasally with live attenuated A/Washington/897/80 (H3N2) or A/California/10/78 (H1N1) c...

journal_title：Journal of clinical microbiology

authors： Clements ML,Murphy BR

更新日期：1986-01-01 00:00:00

abstract：:The single-nucleotide variation 823C to T (His275Tyr), responsible for oseltamivir drug resistance has been detected in some isolates of the influenza A/H1N1/2009 virus. Early detection of the presence of this oseltamivir-resistant strain allows prompt consideration of alternative treatment options. An isolated-probe-...

journal_title：Journal of clinical microbiology

authors： Lee HK,Lee CK,Loh TP,Tang JW,Tambyah PA,Koay ES

更新日期：2011-10-01 00:00:00

abstract：:Nineteen multidrug-resistant (MDR) Mycobacterium complex strains isolated in a nosocomial outbreak were characterized at the molecular level. The strains were microbiologically characterized as Mycobacterium bovis. The mpt40 sequence was not present in chromosomal DNA from these strains, supporting the fact that they ...

journal_title：Journal of clinical microbiology

authors： Blázquez J,Espinosa de Los Monteros LE,Samper S,Martín C,Guerrero A,Cobo J,Van Embden J,Baquero F,Gómez-Mampaso E

更新日期：1997-06-01 00:00:00

abstract：:The correlation between response to antiviral therapy and pretreatment viral load in patients with chronic hepatitis C has prompted the development of quantitative assays to measure viral load. The aim of our study was to assess the clinical relevance of the newly developed semiautomated PCR system COBAS HCV MONITOR v...

journal_title：Journal of clinical microbiology

authors： Martinot-Peignoux M,Le Breton V,Fritsch S,Le Guludec G,Labouret N,Keller F,Marcellin P

更新日期：2000-07-01 00:00:00

abstract：:Between 1998 and 2007, records from 33 patients with cutaneous diphtheria from Vancouver's inner city were reviewed. Cases were associated with injection drug use and poverty. Coinfections with Staphylococcus aureus, Streptococcus pyogenes, and Arcanobacterium haemolyticum occurred. Corynebacterium diphtheriae is ende...

journal_title：Journal of clinical microbiology

authors： Lowe CF,Bernard KA,Romney MG

更新日期：2011-07-01 00:00:00

abstract：:In comparative studies, Hemo-De (PMP Medical Industries, Inc., Irving, Tex.) was found to be a suitable replacement for ethyl acetate in the Formalin-ethyl acetate concentration technique. With essentially equivalent recovery rates for both procedures, the Formalin-Hemo-De concentration technique is considered to be t...

journal_title：Journal of clinical microbiology

authors： Neimeister R,Logan AL,Gerber B,Egleton JH,Kleger B

更新日期：1987-02-01 00:00:00

abstract：:Two commercial assays that detect Mycobacterium tuberculosis complex (MTB) in clinical specimens by rRNA target amplification (AMTDII) and ligase chain reaction (LCx) were evaluated. The tests were applied to 457 respiratory (n = 273) and extrapulmonary (n = 184) specimens collected from 357 patients. The results were...

journal_title：Journal of clinical microbiology

authors： Piersimoni C,Callegaro A,Scarparo C,Penati V,Nista D,Bornigia S,Lacchini C,Scagnelli M,Santini G,De Sio G

更新日期：1998-12-01 00:00:00

abstract：:The use of molecular identification techniques has revealed an increasing number of new species within Aspergillus section Terrei. We phenotyped a set of 26 clinical isolates that showed genetic differences from Aspergillus terreus sensu stricto by analyzing sequences from PCR-amplified β-tubulin and calmodulin genes ...

journal_title：Journal of clinical microbiology

authors： Guinea J,Sandoval-Denis M,Escribano P,Peláez T,Guarro J,Bouza E

更新日期：2015-02-01 00:00:00

abstract：:A commercial multiplex PCR (hyplex SuperBug ID) was tested with a collection of 132 clinical Enterobacteriaceae strains producing different carbapenemases. The sensitivity for the detection of KPC-, VIM-, NDM-, and OXA-48-encoding genes was 100%, whereas two IMP variants were missed. ...

journal_title：Journal of clinical microbiology

authors： Kaase M,Szabados F,Wassill L,Gatermann SG

更新日期：2012-09-01 00:00:00

abstract：:Three hundred thirty-one yeast and yeast-like isolates were cultivated on eosin methylene blue agar. While the sensitivity rate for Candida kefyr isolates producing a metallic green sheen was 81.8%, the high positive predictive value (100%) for yeast isolates with this phenotype belonging to C. kefyr suggests that the...

journal_title：Journal of clinical microbiology

authors： Munson EL,Troy DR,Weber JK,Messer SA,Pfaller MA

更新日期：2002-11-01 00:00:00

abstract：:Leifsonia aquatica is an aquatic bacterium that is typically found in environmental water habitats. Infections due to L. aquatica are rare and commonly catheter associated in immunocompromised patients. We report the first case of an acute septicemia caused by L. aquatica in a healthy immunocompetent host after cryope...

journal_title：Journal of clinical microbiology

authors： Han L,Lei JE,Wang X,Guo LT,Kang QY,He L,Xu JR

更新日期：2013-11-01 00:00:00

abstract：:Blood culture contamination greatly affects clinical decisions. Hence, it is of interest to assess the influence of factors such as the volume of blood drawn and the site of blood draw on the rates of blood culture contamination. In a retrospective study, blood cultures from infants and children up to 18 years of age ...

journal_title：Journal of clinical microbiology

authors： Gonsalves WI,Cornish N,Moore M,Chen A,Varman M

更新日期：2009-11-01 00:00:00