Detection of carbapenemases in Enterobacteriaceae by a commercial multiplex PCR.

abstract：:An immunoglobulin M (IgM) capture enzyme immunoassay was used to detect major overlapping outbreaks of disease in South Australia caused by coxsackieviruses B5 (CBV-5) and B6 (CBV-6). CBV-5-specific IgM was detected in patients presenting in spring 1992 with acute febrile illnesses, rash, severe acute respiratory dise...

journal_title：Journal of clinical microbiology

authors： Goldwater PN

更新日期：1995-06-01 00:00:00

abstract：:Pulsed-field gel electrophoresis (PFGE) of 61 Salmonella enterica serotype Paratyphi C isolates from six countries gave five distinct clusters. Twenty-four isolates from five countries were susceptible to 10 antimicrobials tested and gave similar restriction endonuclease digest patterns of the 38-MDa plasmid. In contr...

journal_title：Journal of clinical microbiology

authors： Kariuki S,Cheesbrough J,Mavridis AK,Hart CA

更新日期：1999-06-01 00:00:00

abstract：:A TaqMan PCR was developed for quantifying early colonizer microorganisms in dental biofilms. To design species-specific primers and TaqMan probes, genomic subtractive hybridization was used. This quantitative assay in combination with subtractive hybridization may be of value in the study of microbial ecosystems cons...

journal_title：Journal of clinical microbiology

authors： Suzuki N,Nakano Y,Yoshida A,Yamashita Y,Kiyoura Y

更新日期：2004-08-01 00:00:00

abstract：:Burkholderia pseudomallei is the causative agent of melioidosis. In northeast Thailand, this gram-negative bacterium is a major cause of mortality from septicemia. The definitive diagnosis of this disease is made by bacterial culture. In this study, we produced a monoclonal antibody (MAb) specific to the 30-kDa protei...

journal_title：Journal of clinical microbiology

authors： Pongsunk S,Thirawattanasuk N,Piyasangthong N,Ekpo P

更新日期：1999-11-01 00:00:00

abstract：:The Vitek MS v2.0 matrix-assisted laser desorption ionization-time of flight mass spectrometry system accurately distinguished Streptococcus pneumoniae from nonpneumococcal S. mitis group species. Only 1 of 116 nonpneumococcal isolates (<1%) was misidentified as S. pneumoniae. None of 95 pneumococcal isolates was misi...

journal_title：Journal of clinical microbiology

authors： Branda JA,Markham RP,Garner CD,Rychert JA,Ferraro MJ

更新日期：2013-09-01 00:00:00

abstract：:We analyzed a collection of 97 well-characterized Burkholderia cepacia genomovar III isolates to evaluate multiple genomic typing systems, including pulsed-field gel electrophoresis (PFGE), BOX-PCR fingerprinting and random amplified polymorphic DNA (RAPD) typing. The typeability, reproducibility, and discriminatory p...

journal_title：Journal of clinical microbiology

authors： Coenye T,Spilker T,Martin A,LiPuma JJ

更新日期：2002-09-01 00:00:00

abstract：:Rapid tests for detection of methicillin-resistant Staphylococcus aureus (MRSA) carriage are important to limit the transmission of MRSA in the health care setting. We evaluated the performance of the BD GeneOhm MRSA real-time PCR assay using a diverse collection of MRSA isolates, mainly from Copenhagen, Denmark, but ...

journal_title：Journal of clinical microbiology

authors： Bartels MD,Boye K,Rohde SM,Larsen AR,Torfs H,Bouchy P,Skov R,Westh H

更新日期：2009-05-01 00:00:00

abstract：:A group of 76 consecutive human immunodeficiency virus (HIV)-positive patients with fever of unknown origin (n = 52) or fever associated with pulmonary diseases was evaluated in order to assess the usefulness of PCR with peripheral blood in the diagnosis and follow-up of visceral leishmaniasis. We identified 10 cases ...

journal_title：Journal of clinical microbiology

authors： Pizzuto M,Piazza M,Senese D,Scalamogna C,Calattini S,Corsico L,Persico T,Adriani B,Magni C,Guaraldi G,Gaiera G,Ludovisi A,Gramiccia M,Galli M,Moroni M,Corbellino M,Antinori S

更新日期：2001-01-01 00:00:00

abstract：:To evaluate the laboratory techniques for subtyping isolates of Salmonella enteritidis, we compared the plasmid profiles (PP), phage types (PT), and antimicrobial susceptibility patterns (AS) of two nationally representative samples of sporadic human S. enteritidis isolates from 1979 (n = 28) and 1984 (n = 37), 43 iso...

journal_title：Journal of clinical microbiology

authors： Rodrigue DC,Cameron DN,Puhr ND,Brenner FW,St Louis ME,Wachsmuth IK,Tauxe RV

更新日期：1992-04-01 00:00:00

abstract：:Mucor ramosissimus Samutsevitsch is presented for the first time as an etiologic agent of cutaneous zygomycosis in a patient with aplastic anemia on immunosuppressive therapy. This report also represents the third case caused by this species reported in the literature. A biopsy taken from a lesion on the patient's thi...

journal_title：Journal of clinical microbiology

authors： Weitzman I,Della-Latta P,Housey G,Rebatta G

更新日期：1993-09-01 00:00:00

abstract：:Clostridium difficile is now recognized as the major agent responsible for nosocomial diarrhea in adults. Among the genotyping methods available, arbitrarily primed PCR (AP-PCR), PCR-ribotyping, and pulsed-field gel electrophoresis (PFGE) have been widely used for investigating outbreaks of C. difficile infections. Ho...

journal_title：Journal of clinical microbiology

authors： Bidet P,Lalande V,Salauze B,Burghoffer B,Avesani V,Delmée M,Rossier A,Barbut F,Petit JC

更新日期：2000-07-01 00:00:00

abstract：:Two multiplex PCR enzyme immunoassays (PCR-EIAs) were developed for Staphylococcus aureus exotoxin gene screening as an alternative to the conventional biological assays, which depend on detectable amounts of toxins produced. One set of oligonucleotide primers and probes was designed to search for enterotoxin A to E g...

journal_title：Journal of clinical microbiology

authors： Becker K,Roth R,Peters G

更新日期：1998-09-01 00:00:00

abstract：:The detection of amplified products resulting from polymerase chain reactions (PCRs) remains a complicated process. To simplify the detection procedures, we developed a colorimetric microtiter plate hybridization assay for the specific detection of 5'-biotinylated PCR fragments of Mycobacterium leprae DNA. For this as...

journal_title：Journal of clinical microbiology

authors： van der Vliet GM,Hermans CJ,Klatser PR

更新日期：1993-03-01 00:00:00

abstract：:RNA of the newly identified human metapneumovirus (HMPV) was detected in nasopharyngeal aspirates of 11 of 63 (17.5%) young children with respiratory tract disease. Markers of infection caused by another member of the Pneumovirinae subfamily of the family Paramyxoviridae, respiratory syncytial virus (RSV), were identi...

journal_title：Journal of clinical microbiology

authors： Viazov S,Ratjen F,Scheidhauer R,Fiedler M,Roggendorf M

更新日期：2003-07-01 00:00:00

abstract：:The pathogenesis of human rhinovirus (HRV) during severe respiratory disease remains undefined; thus, we aimed to explore the relationship between the HRV molecular subtyping results obtained during severe and asymptomatic childhood infections. Nasopharyngeal/oropharyngeal swabs from children (1 to 59 months of age) h...

journal_title：Journal of clinical microbiology

authors： Baillie VL,Moore DP,Mathunjwa A,Morailane P,Simões EAF,Madhi SA

更新日期：2019-08-26 00:00:00

abstract：:The rise of imported malaria cases and the high fatality rate in Europe make the search for new and easy diagnostic methods necessary. Rapid diagnosis tests (RDTs) are, in part, developed to cover the lack of diagnosis experience. Unfortunately, our data suggest that the accuracy of RDTs is insufficient and could incr...

journal_title：Journal of clinical microbiology

authors： Rubio JM,Buhigas I,Subirats M,Baquero M,Puente S,Benito A

更新日期：2001-07-01 00:00:00

abstract：:Of 1,510 sputum samples examined from 1,016 patients with bronchopulmonary disorders, Nocardia asteroides was isolated from 67 samples by paraffin baiting, as compared with only 30 isolations by the conventional technique of culturing on Sabouraud dextrose agar. This higher efficacy of the paraffin bait technique was ...

journal_title：Journal of clinical microbiology

authors： Singh M,Sandhu RS,Randhawa HS

更新日期：1987-01-01 00:00:00

abstract：:Procedures for differentiation between wild and vaccine-derived strains of poliovirus are required, particularly in countries where wild and vaccine-related strains coexist. For this differentiation, we tested the method of Inouye and Hondo (S. Inouye and R. Hondo, Arch. Virol. 129:311-316, 1993) for discrimination of...

journal_title：Journal of clinical microbiology

authors： Takeda N,Sakae K,Agboatwalla M,Isomura S,Hondo R,Inouye S

更新日期：1994-01-01 00:00:00

abstract：:Yersinia enterocolitica biotype 1, serotype O:21 was isolated from feces or rectal washings of three members of one family in northwestern Saskatchewan. The three isolates gave positive pathogenicity tests in guinea pigs with cultures grown at 22 degrees C as inoculum. All three cases showed clinical symptoms consiste...

journal_title：Journal of clinical microbiology

authors： Martin T,Kasian GF,Stead S

更新日期：1982-10-01 00:00:00

abstract：:Etest is a new concept for MIC determinations for antimicrobial agents that is based on a predefined antibiotic gradient on a plastic strip calibrated with a continuous logarithmic MIC scale covering 15 twofold dilutions. Etest was compared with a reference agar dilution method for susceptibility testing of clinical i...

journal_title：Journal of clinical microbiology

authors： Hoffner SE,Klintz L,Olsson-Liljequist B,Bolmström A

更新日期：1994-08-01 00:00:00

abstract：:Routine bacterial cultures of corneal scrapings from seven cats with either ulcerative feline keratitis, keratomalacia, or both yielded colonies which were identified by 16S rRNA gene sequencing as Mycoplasma felis (six cases) and Mycoplasma gateae (one case). Identification of the pathogens allowed the use of less em...

journal_title：Journal of clinical microbiology

authors： Gray LD,Ketring KL,Tang YW

更新日期：2005-07-01 00:00:00

abstract：:Monoclonal antibodies to K88ac and K88ab were used in enzyme-linked immunosorbent assays on Escherichia coli cultures known to produce K88 pili. A total of 415 K88-positive E. coli isolates from nine states were all found to be the K88ac variant. The cultures tested were isolated during the years 1976 to 1985. ...

journal_title：Journal of clinical microbiology

authors： Westerman RB,Mills KW,Phillips RM,Fortner GW,Greenwood JM

更新日期：1988-01-01 00:00:00

abstract：:Multidrug-resistant tuberculous meningitis is fatal without rapid diagnosis and use of second-line therapy. It is more common in human immunodeficiency virus (HIV)-positive patients. Beijing genotype strains of Mycobacterium tuberculosis are associated with drug resistance, particularly multidrug resistance, and their...

journal_title：Journal of clinical microbiology

authors： Caws M,Thwaites G,Stepniewska K,Nguyen TN,Nguyen TH,Nguyen TP,Mai NT,Phan MD,Tran HL,Tran TH,van Soolingen D,Kremer K,Nguyen VV,Nguyen TC,Farrar J

更新日期：2006-11-01 00:00:00

abstract：:Metagenomic next-generation sequencing (mNGS) of samples from 15 patients with documented Zika virus (ZIKV) infection in Bahia, Brazil, from April 2015 to January 2016 identified coinfections with chikungunya virus (CHIKV) in 2 of 15 ZIKV-positive cases by PCR (13.3%). While generally nonspecific, the clinical present...

journal_title：Journal of clinical microbiology

authors： Sardi SI,Somasekar S,Naccache SN,Bandeira AC,Tauro LB,Campos GS,Chiu CY

更新日期：2016-09-01 00:00:00

abstract：:Todd-Hewitt broth (THB) containing colistin and nalidixic acid was compared with four other media with respect to efficacy of isolation of group B streptocci. It was effective as plain THB, THB with gentamicin and nalidixic acid and blood, and THB with colistin, nalidixic acid, and blood. THB with gentamicin and nalid...

journal_title：Journal of clinical microbiology

authors： Fenton LJ,Harper MH

更新日期：1979-02-01 00:00:00

abstract：:Genotypic typing by restriction fragment length polymorphism and pulsed-field gel electrophoresis showed that two neonates in a neonatal ward were infected with the same Campylobacter jejuni strain. Isolates from the mother and brother of the index patient were identical to each other but distinct from the neonatal ty...

journal_title：Journal of clinical microbiology

authors： Llovo J,Mateo E,Muñoz A,Urquijo M,On SL,Fernández-Astorga A

更新日期：2003-08-01 00:00:00

abstract：:Results of a 2003 survey carried out in Italy to evaluate the prevalence of extended-spectrum beta-lactamase (ESBL)-producing enterobacteria are presented. Eleven Italian Microbiology Laboratories investigated 9,076 consecutive nonreplicate isolates (inpatients, 6,850; outpatients, 2,226). ESBL screening was performed...

journal_title：Journal of clinical microbiology

authors： Luzzaro F,Mezzatesta M,Mugnaioli C,Perilli M,Stefani S,Amicosante G,Rossolini GM,Toniolo A

更新日期：2006-05-01 00:00:00

abstract：:The performance characteristics of three real-time influenza A/B virus reverse transcription-PCR (RT-PCR) assays and two real-time 2009 H1N1 RT-PCR assays were evaluated using previously characterized clinical specimens. A total of 150 respiratory specimens from children (30 influenza A/H1 virus-, 30 influenza A/H3 vi...

journal_title：Journal of clinical microbiology

authors： Selvaraju SB,Selvarangan R

更新日期：2010-11-01 00:00:00

abstract：:Rhodococcus equi infection is increasing in regions with high HIV prevalence worldwide. The microbiological features and clinical mimicry of tuberculosis infection pose diagnostic challenges in high-tuberculosis-incidence settings. We present two HIV-associated cases of R. equi infection from Vietnam and discuss the u...

journal_title：Journal of clinical microbiology

authors： Le T,Cash-Goldwasser S,Tho PV,Lan NP,Campbell JI,van Doorn HR,Lam NT,Trung NV,Trinh DT,Van Kinh N,Wertheim HF

更新日期：2015-04-01 00:00:00

abstract：:Capnocytophaga canimorsus is a dog oral commensal that causes rare but severe infections in humans. C. canimorsus was recently shown to be endowed with a capsular polysaccharide implicated in resistance to the innate immune system of the host. Here, we developed the first C. canimorsus capsular serotyping scheme. We d...

journal_title：Journal of clinical microbiology

authors： Hess E,Renzi F,Koudad D,Dol M,Cornelis GR

更新日期：2017-06-01 00:00:00