Performance of the Vitek MS v2.0 system in distinguishing Streptococcus pneumoniae from nonpneumococcal species of the Streptococcus mitis group.

abstract：:Bacteriological and morphological studies of 38 brown pigment common duct stones were performed. Stone cultures were positive for bacteria in 80.5% of those studied. Enterococci were the most common organisms that were isolated. Scanning electron microscopy showed the presence of bacteria in 84.2% of the stones. The b...

journal_title：Journal of clinical microbiology

authors： Leung JW,Sung JY,Costerton JW

更新日期：1989-05-01 00:00:00

abstract：:In 32 patients for whom highly active antiretroviral therapy was failing, a good agreement between drug resistance-associated mutations in plasma and peripheral blood mononuclear cells (PBMCs) was found (k = 0.85). The mutations with the lowest agreement were 20R, 63P, and 84V in the protease gene and 184V in the reve...

journal_title：Journal of clinical microbiology

authors： Sarmati L,Nicastri E,Uccella I,D'Ettorre G,Parisi SG,Palmisano L,Galluzzo C,Concia E,Vullo V,Vella S,Andreoni M

更新日期：2003-04-01 00:00:00

abstract：:The performance of three line blot assays (LBAs), the Linear Array HPV genotyping assay (LA) (Roche Diagnostics), INNO-LiPA HPV Genotyping Extra (LiPA) (Innogenetics), and the reverse hybridization assay (RH) (Qiagen), was evaluated using quantitated whole genomic human papillomavirus (HPV) plasmids (types 6, 11, 16, ...

journal_title：Journal of clinical microbiology

authors： Steinau M,Onyekwuluje JM,Scarbrough MZ,Unger ER,Dillner J,Zhou T

更新日期：2012-05-01 00:00:00

abstract：:We investigated whether methicillin (meticillin)-resistant Staphylococcus aureus (MRSA) isolates with low-level mupirocin resistance can serve as recipients of a pSK41-like plasmid conferring high-level mupirocin resistance without substantial fitness cost. Our results suggest that acquisition of the plasmid conferrin...

journal_title：Journal of clinical microbiology

authors： Mongkolrattanothai K,Pumfrey L,Mankin P,Gray BM

更新日期：2009-12-01 00:00:00

abstract：:Longitudinal data on the E6/E7 mRNA-based Aptima human papillomavirus (AHPV) assay exceeding three years in comparison to the gold standard Digene Hybrid Capture 2 (HC2) test are not available. We previously reported the cross-sectional data of the German AHPV Screening Trial (GAST) in which 10,040 women were recruite...

journal_title：Journal of clinical microbiology

authors： Iftner T,Neis KJ,Castanon A,Landy R,Holz B,Woll-Herrmann A,Iftner A,Staebler A,Wallwiener D,Hann von Weyhern C,Neis F,Haedicke-Jarboui J,Martus P,Brucker S,Henes M,Sasieni P

更新日期：2019-01-02 00:00:00

abstract：:Alere i RSV is a novel rapid test which applies a nicking enzyme amplification reaction to detect respiratory syncytial virus in point-of-care settings. In this study, we evaluated the Alere i RSV assay by using frozen nasopharyngeal swab samples that were collected in viral transport medium from children hospitalized...

journal_title：Journal of clinical microbiology

authors： Peters RM,Schnee SV,Tabatabai J,Schnitzler P,Pfeil J

更新日期：2017-04-01 00:00:00

abstract：:For testing bacterial susceptibility to PD131628, a 5-micrograms disk and the following tentative interpretive criteria may be used: > or = 19 mm for susceptible (MIC, < or = 1.0 micrograms/ml), 16 to 18 mm for intermediate (MIC, 2.0 micrograms/ml), and < or = 15 mm for resistant (MIC, > or = 4.0 micrograms/ml). For s...

journal_title：Journal of clinical microbiology

authors： Barry AL,Fuchs PC,Allen SD,Tenover FC,Jorgensen JH,Reller LB

更新日期：1995-01-01 00:00:00

abstract：:Optimal conditions for demonstrating the presence of infectious human immunodeficiency virus in peripheral blood mononuclear cells (PMCs) from seropositive individuals involved cocultivation of infected cells with phytohemagglutinin-stimulated PMCs from seronegative donors in the presence of 2 micrograms of Polybrene ...

journal_title：Journal of clinical microbiology

authors： Castro BA,Weiss CD,Wiviott LD,Levy JA

更新日期：1988-11-01 00:00:00

abstract：:Nosocomial oxacillin-resistant Staphylococcus aureus (ORSA) bloodstream isolates were tested to determine the prevalence of vancomycin heteroresistance. We screened 619 ORSA nosocomial bloodstream isolates from 36 hospitals between 1997 and 2000. Only one isolate exhibiting heterotypic resistance was detected. Thus, v...

journal_title：Journal of clinical microbiology

authors： Tallent SM,Bischoff T,Climo M,Ostrowsky B,Wenzel RP,Edmond MB

更新日期：2002-06-01 00:00:00

abstract：:Transfusion-acquired cytomegalovirus (CMV) infections should be prevented in seronegative immunocompromised patients by providing blood products from donors who are also seronegative. Latex agglutination was investigated as a simple and rapid method for detecting antibody against CMV. Latex beads were coated with CMV ...

journal_title：Journal of clinical microbiology

authors： Adler SP,McVoy M,Biro VG,Britt WJ,Hider P,Marshall D

更新日期：1985-07-01 00:00:00

abstract：:A rapid and accurate method to identify the species and antibiotic resistance of Candida spp. in blood is vital to increase the survival rates of patients with bloodstream infections. However, the extremely low levels of Candida spp. in blood make rapid diagnosis by standard blood culture difficult. In this study, we ...

journal_title：Journal of clinical microbiology

authors： Chen XL,Zheng H,Li WG,Zhong YH,Chen XP,Lu JX

更新日期：2020-03-25 00:00:00

abstract：:We describe a multiplex reverse transcription-PCR (m-RT-PCR) assay that is able to detect and differentiate all known human parainfluenza viruses (HPIVs). Serial dilution experiments with reference strains that compared cell culture isolation and m-RT-PCR showed sensitivities ranging from 0.0004 50% tissue culture inf...

journal_title：Journal of clinical microbiology

authors： Aguilar JC,Pérez-Breña MP,García ML,Cruz N,Erdman DD,Echevarría JE

更新日期：2000-03-01 00:00:00

abstract：:Mycoplasma pneumoniae is a common cause of community-acquired pneumonia and lower-respiratory-tract infections. Diagnosis has traditionally been obtained by serological diagnosis, but increasingly, molecular techniques have been applied. However, the number of studies actually comparing these assays is limited. The de...

journal_title：Journal of clinical microbiology

authors： Templeton KE,Scheltinga SA,Graffelman AW,Van Schie JM,Crielaard JW,Sillekens P,Van Den Broek PJ,Goossens H,Beersma MF,Claas EC

更新日期：2003-09-01 00:00:00

abstract：:Rapid and reliable detection of methicillin-resistant Staphylococcus aureus (MRSA) carriers is crucial for the effective control of MRSA transmission in healthcare facilities. The aim of this study was to verify the performance of the IDI-MRSA real-time PCR assay for direct MRSA detection in diverse mucocutaneous swab...

journal_title：Journal of clinical microbiology

authors： de San N,Denis O,Gasasira MF,De Mendonça R,Nonhoff C,Struelens MJ

更新日期：2007-04-01 00:00:00

abstract：:Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been found to be an accurate, rapid, and inexpensive method for the identification of bacteria and yeasts. Previous evaluations have compared the accuracy, time to identification, and costs of the MALDI-TOF MS method agains...

journal_title：Journal of clinical microbiology

authors： Tan KE,Ellis BC,Lee R,Stamper PD,Zhang SX,Carroll KC

更新日期：2012-10-01 00:00:00

abstract：:A new self-contained medium system for the cultivation of Neisseria gonorrhoeae was compared to modified Thayer-Martin medium for the diagnosis of gonorrhea from endocervical specimens. There was no difference in the ability of the two methods to support the growth of N. gonorrhoeae. ...

journal_title：Journal of clinical microbiology

authors： Beverly A,Bailey-Griffin JR,Schwebke JR

更新日期：2000-10-01 00:00:00

abstract：:The etiology of the purple urine bag syndrome (PUBS), in which the urinary catheter bag of some elderly patients develops intense purple coloration, was studied. The purple was found to be a mixture of indirubin dissolved in the plastic and indigo on its surface. Six patients with PUBS were studied, and Providencia st...

journal_title：Journal of clinical microbiology

authors： Dealler SF,Hawkey PM,Millar MR

更新日期：1988-10-01 00:00:00

abstract：:We report a study of 1,953 patients whose laboratory records from 1972 through 1983 at the Massachusetts Mycobacteria Reference Laboratory indicated the isolation of Mycobacterium avium complex (MAC) organisms. At least one clinical specimen from each patient during this period exhibited the organism. The incidence of...

journal_title：Journal of clinical microbiology

authors： du Moulin GC,Sherman IH,Hoaglin DC,Stottmeier KD

更新日期：1985-07-01 00:00:00

abstract：:Lipoglycans from mycoplasmas possess the ability to induce the elaboration of procoagulant activity. This represents a third endotoxic property for these polymers, in addition to the pyrogenicity and clotting of Limulus lysate reported previously. Lipoglycans from three species of Acholeplasma (A. axanthum, A. granula...

journal_title：Journal of clinical microbiology

authors： Miragliotta G,Barone G,Monno RA,Fumarola D,Smith PF

更新日期：1987-07-01 00:00:00

abstract：:Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry with a Bruker Daltonics microflex LT system was applied to 90 well-characterized catalase-negative, Gram-positive cocci not belonging to the streptococci or enterococci. Biotyper version 2.0.43.1 software was used singly or in com...

journal_title：Journal of clinical microbiology

authors： Christensen JJ,Dargis R,Hammer M,Justesen US,Nielsen XC,Kemp M,Danish MALDI-TOF MS Study Group.

更新日期：2012-05-01 00:00:00

abstract：:During the course of a molecular epidemiology study of mechanisms of antibiotic resistance in the area served by our hospital (516,000 inhabitants), we isolated the gene encoding CTX-M-14 beta-lactamase. Thirty clinical strains (27 Escherichia coli and 3 Klebsiella pneumoniae isolates) with a phenotype of extended-spe...

journal_title：Journal of clinical microbiology

authors： Bou G,Cartelle M,Tomas M,Canle D,Molina F,Moure R,Eiros JM,Guerrero A

更新日期：2002-11-01 00:00:00

abstract：:Sequential specimens from nine allograft recipients were examined by using a variety of methods to detect primary cytomegalovirus (CMV) infection as rapidly as possible posttransplantation. Sera were examined for immunoglobulin G (IgG) and IgM antibodies by immunoblotting, enzyme immunoassay, and immunofluorescence an...

journal_title：Journal of clinical microbiology

authors： Miller H,McCulloch B,Landini MP,Rossier E

更新日期：1989-12-01 00:00:00

abstract：:A biotin-avidin-amplified indirect immunofluorescence method was used to demonstrate specific serum immunoglobulin M (IgM) antibodies in nephropathia epidemica, the Scandinavian type of hemorrhagic fever with renal syndrome. The antigen in the test was the cross-reacting agent of Korean hemorrhagic fever, Hantaan viru...

journal_title：Journal of clinical microbiology

authors： Settergren B,Juto P,Wadell G

更新日期：1987-06-01 00:00:00

abstract：:An isolate of Proteus mirabilis recovered from blood cultures of a diabetic patient was shown to be resistant to imipenem, meropenem, and ertapenem by disk diffusion susceptibility testing. Amplification of whole-cell and/or plasmid DNA recovered from the isolate with primers specific for the bla(KPC) carbapenemase ge...

journal_title：Journal of clinical microbiology

authors： Tibbetts R,Frye JG,Marschall J,Warren D,Dunne W

更新日期：2008-09-01 00:00:00

abstract：:Currently, no agar-based susceptibility testing method has been standardized for testing dermatophytes. We describe a newly developed agar-based method employing disk diffusion assay to test the susceptibility of 47 isolates of dermatophytes against 8 antifungals. Our results show that the method is reproducible, is s...

journal_title：Journal of clinical microbiology

authors： Nweze EI,Mukherjee PK,Ghannoum MA

更新日期：2010-10-01 00:00:00

abstract：:We isolated and characterized a Vibrio cholerae O139 Bengal-specific DNA region by arbitrary PCR. The fragment contains open reading frames encoding two potential glycosyltransferases possibly involved in capsular polysaccharide or lipopolysaccharide biosynthesis. In order to evaluate the possibility that this region ...

journal_title：Journal of clinical microbiology

authors： Falklind S,Stark M,Albert MJ,Uhlén M,Lundeberg J,Weintraub A

更新日期：1996-12-01 00:00:00

abstract：:A total of 800 colonies suggestive of Salmonella, Shigella, or Yersinia species isolated on stool differential agar media were inoculated onto both conventional biochemical test media (triple sugar iron agar, urea agar, and phenylalanine agar) and Entero Pathogen Screen cards of the AutoMicrobic system (Vitek Systems,...

journal_title：Journal of clinical microbiology

authors： Villasante PA,Agulla A,Merino FJ,Pérez T,Ladrón de Guevara C,Velasco AC

更新日期：1987-03-01 00:00:00

abstract：:An atypical toxin variant of Clostridium botulinum (strain 657) was isolated from the feces of a 6-week-old female infant whose symptoms and clinical history were consistent with infant botulism. Toxin detected in the feces and the toxin produced by isolates from the feces and from two rectal swabs could be neutralize...

journal_title：Journal of clinical microbiology

authors： Hatheway CL,McCroskey LM,Lombard GL,Dowell VR Jr

更新日期：1981-12-01 00:00:00

abstract：:The development and application of nucleic acid sequence-based amplification (NASBA) assays for the detection of West Nile (WN) and St. Louis encephalitis (SLE) viruses are reported. Two unique detection formats were developed for the NASBA assays: a postamplification detection step with a virus-specific internal capt...

journal_title：Journal of clinical microbiology

authors： Lanciotti RS,Kerst AJ

更新日期：2001-12-01 00:00:00

abstract：:The pathogenic Neisseria species N. meningitidis and N. gonorrhoeae possess an outer membrane lipoprotein, designated Lip, which is present in all strains tested. The predicted protein sequence of Lip consists of a consensus AAEAP amino acid repeat. The objective of this study was to determine the feasibility of using...

journal_title：Journal of clinical microbiology

authors： Trees DL,Schultz AJ,Knapp JS

更新日期：2000-08-01 00:00:00