Abstract:
:Mycoplasma pneumoniae is a common cause of community-acquired pneumonia and lower-respiratory-tract infections. Diagnosis has traditionally been obtained by serological diagnosis, but increasingly, molecular techniques have been applied. However, the number of studies actually comparing these assays is limited. The development of a novel duplex real-time PCR assay for detection of M. pneumoniae in the presence of an internal control real-time PCR is described. In addition, real-time nucleic acid sequence-based amplification (NASBA) on an iCycler apparatus is evaluated. These assays were compared to serology and a conventional PCR assay for 106 clinical samples from patients with lower-respiratory-tract infection. Of the 106 samples, 12 (11.3%) were positive by all the molecular methods whereas serology with acute sample and convalescent samples detected 6 (5.6%) and 9 (8.5%), respectively. Clinical symptoms of the patients with Mycoplasma-positive results were compared to those of the other patients with lower-respiratory-tract infections, and it was found that the results for mean lower age numbers as well as the presence of chills, increased erythrocyte sedimentation rate, and raised C-reactive protein levels showed significant differences. Molecular methods are superior for diagnosis of M. pneumoniae, providing more timely diagnosis. In addition, using real-time methods involves less hands-on time and affords the ability to monitor the reaction in the same tube.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Templeton KE,Scheltinga SA,Graffelman AW,Van Schie JM,Crielaard JW,Sillekens P,Van Den Broek PJ,Goossens H,Beersma MF,Claas ECdoi
10.1128/jcm.41.9.4366-4371.2003subject
Has Abstractpub_date
2003-09-01 00:00:00pages
4366-71issue
9eissn
0095-1137issn
1098-660Xjournal_volume
41pub_type
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