Evaluation of the novel Helicobacter pylori ClariRes real-time PCR assay for detection and clarithromycin susceptibility testing of H. pylori in stool specimens from symptomatic children.

abstract：:Methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of hospital-acquired and life-threatening infections. Active surveillance programs for MRSA utilize either molecular or culture-based methods. A prospective study was performed to compare the performance of selective and differential chromogenic media...

journal_title：Journal of clinical microbiology

authors： Denys GA,Renzi PB,Koch KM,Wissel CM

更新日期：2013-01-01 00:00:00

abstract：:Between January 1988 and December 1989 Aeromonas species were isolated from 45 (1.8%) of 2,480 patients with acute gastroenteritis. No other bacterial enteric pathogens were found in any of these 45 patients. Of the 45 Aeromonas isolates, 35 strains (77.8%) were Aeromonas hydrophila, 7 (15.5%) were Aeromonas sobria, a...

journal_title：Journal of clinical microbiology

authors： Deodhar LP,Saraswathi K,Varudkar A

更新日期：1991-05-01 00:00:00

abstract：:Analysis of hospital-acquired methicillin-resistant Staphylococcus aureus strains isolated from a tertiary public hospital in Singapore revealed that multisusceptible strains had gradually started to replace the endemic multiresistant strain (ST239-MRSA-III) since 2002. Molecular typing showed that this was a predomin...

journal_title：Journal of clinical microbiology

authors： Hsu LY,Koh TH,Singh K,Kang ML,Kurup A,Tan BH

更新日期：2005-06-01 00:00:00

abstract：:The TRUGENE HIV-1 Genotyping Kit and OpenGene DNA Sequencing System are designed to sequence the protease (PR)- and reverse transcriptase (RT)-coding regions of human immunodeficiency virus type 1 (HIV-1) pol. Studies were undertaken to determine the accuracy of this assay system in detecting resistance-associated mut...

journal_title：Journal of clinical microbiology

authors： Kuritzkes DR,Grant RM,Feorino P,Griswold M,Hoover M,Young R,Day S,Lloyd RM Jr,Reid C,Morgan GF,Winslow DL

更新日期：2003-04-01 00:00:00

abstract：:Identification of chromosomal markers for rapid detection of Bacillus anthracis is difficult because significant chromosomal homology exists among B. anthracis, Bacillus cereus, and Bacillus thuringiensis. We evaluated the bacterial gyrA gene as a potential chromosomal marker for B. anthracis. A real-time PCR assay wa...

journal_title：Journal of clinical microbiology

authors： Hurtle W,Bode E,Kulesh DA,Kaplan RS,Garrison J,Bridge D,House M,Frye MS,Loveless B,Norwood D

更新日期：2004-01-01 00:00:00

abstract：:The in vitro susceptibilities of 66 molecularly identified strains of the Mucorales to eight antifungals (amphotericin B, terbinafine, itraconazole, posaconazole, voriconazole, caspofungin, micafungin, and 5-fluorocytosine) were tested. Molecular phylogeny was reconstructed based on the nuclear ribosomal large subunit...

journal_title：Journal of clinical microbiology

authors： Vitale RG,de Hoog GS,Schwarz P,Dannaoui E,Deng S,Machouart M,Voigt K,van de Sande WW,Dolatabadi S,Meis JF,Walther G

更新日期：2012-01-01 00:00:00

abstract：:Accurate and internationally comparable human papillomavirus (HPV) DNA genotyping is essential both for evaluation of HPV vaccines and for effective monitoring and implementation of vaccination programs. The World Health Organization (WHO) HPV Laboratory Network (LabNet) regularly issues international proficiency stud...

journal_title：Journal of clinical microbiology

authors： Eklund C,Forslund O,Wallin KL,Zhou T,Dillner J,WHO Human Papillomavirus Laboratory Network.

更新日期：2012-07-01 00:00:00

abstract：:A commercial DNA probe kit designed to detect rRNA from legionellae was evaluated for its ability to correctly discriminate between legionellae and non-legionellae taken from culture plates. The probe kit, made by the Gen-Probe Corp. (San Diego, Calif.), was radiolabeled with 125I, and probe bacterial RNA hybridizatio...

journal_title：Journal of clinical microbiology

authors： Edelstein PH

更新日期：1986-03-01 00:00:00

abstract：:A prospective study was performed comparing the fluorescent antibody to membrane antigen (FAMA) test and the enzyme-linked immunosorbent assay (ELISA) for identifying susceptibility and seroconversion to varicella-zoster virus (VZV) infection. A total of 75 sera were collected from index cases and from sibling and par...

journal_title：Journal of clinical microbiology

authors： Iltis JP,Castellano GA,Gerber P,Le C,Vujcic LK,Quinnan GV Jr

更新日期：1982-11-01 00:00:00

abstract：:Penicilliosis, caused by the dimorphic fungus Penicillium marneffei, is an important opportunistic systemic fungal infection affecting immunocompromised individuals living in areas where penicilliosis is endemic. We have demonstrated previously that a urinary enzyme-linked immunosorbent assay (ELISA) with purified rab...

journal_title：Journal of clinical microbiology

authors： Desakorn V,Simpson AJ,Wuthiekanun V,Sahassananda D,Rajanuwong A,Pitisuttithum P,Howe PA,Smith MD,White NJ

更新日期：2002-09-01 00:00:00

abstract：:Molecular techniques such as spa typing and multilocus sequence typing use DNA sequence data for differentiating Staphylococcus aureus isolates. Although spa typing is capable of detecting both genetic micro- and macrovariation, it has less discriminatory power than the more labor-intensive pulsed-field gel electropho...

journal_title：Journal of clinical microbiology

authors： Koreen L,Ramaswamy SV,Naidich S,Koreen IV,Graff GR,Graviss EA,Kreiswirth BN

更新日期：2005-08-01 00:00:00

abstract：:Between November 1983 and January 1984, 13 cases of Candida parapsilosis endophthalmitis occurred in Florida, Georgia, and Tennessee in patients who had had an intraocular lens implantation (IOLI) or cataract extraction with an IOLI. This outbreak followed the introduction in July 1983 of a new brand of balanced salt ...

journal_title：Journal of clinical microbiology

authors： McCray E,Rampell N,Solomon SL,Bond WW,Martone WJ,O'Day D

更新日期：1986-10-01 00:00:00

abstract：:Many bunyaviruses of the Bunyamwera and California serogroups are medically important human pathogens. The development of an effective technique to detect the viruses by using molecular biologic tools, such as PCR, improves not only clinical diagnosis but also virologic surveillance of mosquito vectors in the field. I...

journal_title：Journal of clinical microbiology

authors： Kuno G,Mitchell CJ,Chang GJ,Smith GC

更新日期：1996-05-01 00:00:00

abstract：:Common causes of chronic diarrhea among travelers worldwide include protozoan parasites. The majority of parasitic infections are caused by Giardia duodenalis, Entamoeba histolytica, Cryptosporidium parvum, and Cryptosporidium hominis Similarly, these species cause the majority of parasitic diarrhea acquired in the Un...

journal_title：Journal of clinical microbiology

authors： Madison-Antenucci S,Relich RF,Doyle L,Espina N,Fuller D,Karchmer T,Lainesse A,Mortensen JE,Pancholi P,Veros W,Harrington SM

更新日期：2016-11-01 00:00:00

abstract：:Transfusion-transmissible infections among 808 blood donors in Ghana were investigated in 1999. Antibody seroprevalences of 3.8, 0.7, 8.4, and 13.5%, respectively, for human immunodeficiency virus, human T-cell lymphotrophic virus type 1, hepatitis C virus (HCV), and Treponema pallidum were obtained. The seroprevalenc...

journal_title：Journal of clinical microbiology

authors： Ampofo W,Nii-Trebi N,Ansah J,Abe K,Naito H,Aidoo S,Nuvor V,Brandful J,Yamamoto N,Ofori-Adjei D,Ishikawa K

更新日期：2002-09-01 00:00:00

abstract：:Fully standardized reproducible and sensitive quantification assays for cytomegalovirus (CMV) are needed to better define thresholds for antiviral therapy initiation and interruption. We evaluated the newly released Abbott RealTime CMV assay for CMV quantification in whole blood (WB) that includes automated extraction...

journal_title：Journal of clinical microbiology

authors： Schnepf N,Scieux C,Resche-Riggon M,Feghoul L,Xhaard A,Gallien S,Molina JM,Socié G,Viglietti D,Simon F,Mazeron MC,Legoff J

更新日期：2013-07-01 00:00:00

abstract：:A procedure for detecting pneumococcal capsular polysaccharides in immune complexes is described. Separation of antigen from immune complexes is achieved by electrophoresis at 56 degrees C. ...

journal_title：Journal of clinical microbiology

authors： Coonrod JD,Leach RP

更新日期：1978-08-01 00:00:00

abstract：:A survey was conducted among Spanish children with gastroenteritis treated in an emergency room. Reverse transcription-PCR with specimens negative for other enteric pathogens was used. The minimum incidence of human calicivirus infection was 7.7%, with Lordsdale as the predominant genotype. The clinical features and s...

journal_title：Journal of clinical microbiology

authors： Roman E,Negredo A,Dalton RM,Wilhelmi I,Sánchez-Fauquier A

更新日期：2002-10-01 00:00:00

abstract：:Rhodotorula infections occur among patients with immunosuppression and/or central venous catheters. Using standardized methods (NCCLS M27-A), we determined the antifungal susceptibilities of 10 Rhodotorula bloodstream infection isolates. Patient information was collected for clinical correlation. The MICs of amphoteri...

journal_title：Journal of clinical microbiology

authors： Zaas AK,Boyce M,Schell W,Lodge BA,Miller JL,Perfect JR

更新日期：2003-11-01 00:00:00

abstract：:Clinical isolates of group B streptococci from body fluids and mucosal surfaces were examined for production of a trypsin-resistant antigen known as R protein. R protein was extracted with 1% trypsin from cells grown in a semidefined medium. The extracts were tested by immunodiffusion in agarose with a panel of antise...

journal_title：Journal of clinical microbiology

authors： Flores AE,Ferrieri P

更新日期：1989-05-01 00:00:00

abstract：:A rapid and simple method for extracting specific cell-free antigens of Coccidioides immitis and Histoplasma capsulatum from agar slant cultures was developed. The extracts were analyzed in immunodiffusion tests for the presence of C. immitis or H. capsulatum specific antigens. These extracts were compared with cultur...

journal_title：Journal of clinical microbiology

authors： Kaufman L,Standard P

更新日期：1978-07-01 00:00:00

abstract：:Sequence-based typing (SBT), analogous to multilocus sequence typing (MLST), is the current "gold standard" typing method for investigation of legionellosis outbreaks caused by Legionella pneumophila However, as common sequence types (STs) cause many infections, some investigations remain unresolved. In this study, va...

journal_title：Journal of clinical microbiology

authors： David S,Mentasti M,Tewolde R,Aslett M,Harris SR,Afshar B,Underwood A,Fry NK,Parkhill J,Harrison TG

更新日期：2016-08-01 00:00:00

abstract：:Infections with human immunodeficiency virus types 1 and 2 (HIV-1 and HIV-2, respectively) are zoonotic infections. In Africa, the potential exists for additional cross-species transmissions from at least 33 different species of simian immunodeficiency virus (SIV)-infected nonhuman primates (NHPs) through hunting and ...

journal_title：Journal of clinical microbiology

authors： Ndongmo CB,Switzer WM,Pau CP,Zeh C,Schaefer A,Pieniazek D,Folks TM,Kalish ML

更新日期：2004-11-01 00:00:00

abstract：:Scedosporium prolificans is a soil saprophyte that is associated with a large variety of infectious processes and with respiratory colonization in immunocompetent and immunocompromised patients. We report the first described case of S. prolificans keratouveitis associated with the intraocular long-term retention of a ...

journal_title：Journal of clinical microbiology

authors： Arthur S,Steed LL,Apple DJ,Peng Q,Howard G,Escobar-Gomez M

更新日期：2001-12-01 00:00:00

abstract：:Ninety-one isolates of nutritionally variant streptococci (NVS) that were previously isolated from the human mouth were regarded as consisting of 7 Streptococcus defectivus isolates, 78 Streptococcus adjacens isolates, and 6 Gemella morbillorum isolates. However, recent references to the taxonomic reclassification of ...

journal_title：Journal of clinical microbiology

authors： Sato S,Kanamoto T,Inoue M

更新日期：1999-08-01 00:00:00

abstract：:A differential staining method employing acridine orange-stained rickettsiae and safranin-stained standardized suspensions of Shigella dysenteriae is described for enumerating rickettsiae harvested during various stages of the growth cycle. ...

journal_title：Journal of clinical microbiology

authors： Silverman DJ,Fiset P,Wisseman CL Jr

更新日期：1979-03-01 00:00:00

abstract：:In order to determine whether mixed infections of human herpesvirus 6B (HHV-6B) occur in immunocompetent and immunocompromised individuals, we examined the copy numbers of telomeric repeat sequences (TRS) of clinical isolates. In clinical isolates obtained from patients with exanthem subitum caused by primary HHV-6B i...

journal_title：Journal of clinical microbiology

authors： Kato Y,Ihira M,Umeda M,Higashimoto Y,Kawamura Y,Ohashi M,Ishi J,Yoshikawa T

更新日期：2014-02-01 00:00:00

abstract：:The use of centrifugation (700 x g, 60 min) in a plaque assay markedly increased (mean, 2.9-fold) the infectivity of all 42 influenza virus strains tested, compared with no centrifugation. Of 13 influenza virus strains isolated from 390 clinical specimens, 9 (69%) were efficiently isolated by the centrifugation assay ...

journal_title：Journal of clinical microbiology

authors： Seno M,Kanamoto Y,Takao S,Takei N,Fukuda S,Umisa H

更新日期：1990-07-01 00:00:00

abstract：:In the treatment of serious bacterial infections, the rapid institution of appropriate antimicrobial chemotherapy may be lifesaving. Choosing the correct antibiotic or combination of antibiotics is becoming very important, as multidrug resistance is found in many pathogens. Using a collection of 75 well-characterized ...

journal_title：Journal of clinical microbiology

authors： Hujer KM,Hujer AM,Endimiani A,Thomson JM,Adams MD,Goglin K,Rather PN,Pennella TT,Massire C,Eshoo MW,Sampath R,Blyn LB,Ecker DJ,Bonomo RA

更新日期：2009-05-01 00:00:00

abstract：:Periodic monitoring of antimicrobial susceptibility of Neisseria gonorrhoeae is essential for early detection of emergence of drug resistance. A total of 343 gonococcal strains isolated from high-risk and general populations in Bangladesh from 1997 to 1999 were studied. The MICs of penicillin, tetracycline, ciprofloxa...

journal_title：Journal of clinical microbiology

authors： Rahman M,Sultan Z,Monira S,Alam A,Nessa K,Islam S,Nahar S,Shama-A-Waris,Alam Khan S,Bogaerts J,Islam N,Albert J

更新日期：2002-06-01 00:00:00