Detecting bunyaviruses of the Bunyamwera and California serogroups by a PCR technique.

abstract：:Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most important causes of hospital infections worldwide. High-level resistance to methicillin is caused by the mecA gene, which encodes an alternative penicillin-binding protein, PBP 2a. To determine the clonal relationships between methicillin-susceptibl...

journal_title：Journal of clinical microbiology

authors： Wielders CL,Fluit AC,Brisse S,Verhoef J,Schmitz FJ

更新日期：2002-11-01 00:00:00

abstract：:A PCR test based on insertion sequence IS1081 was developed to detect Mycobacterium tuberculosis complex organisms in the peripheral blood. The method was applied to blood samples from immunocompetent individuals with localized pulmonary tuberculosis. Seven of 16 (43.75%) blood samples were found to be positive for th...

journal_title：Journal of clinical microbiology

authors： Ahmed N,Mohanty AK,Mukhopadhyay U,Batish VK,Grover S

更新日期：1998-10-01 00:00:00

abstract：:An in situ DNA hybridization kit for cytomegalovirus (CMV) was evaluated for the detection of CMV in centrifugation culture. Of 61 clinical specimens, 17 (27.8%) were positive for CMV by monoclonal antibody staining following centrifugation. Of the 17 positive specimens, 15 were detected by DNA hybridization (24.5%). ...

journal_title：Journal of clinical microbiology

authors： Gleaves CA,Hursh DA,Rice DH,Meyers JD

更新日期：1989-01-01 00:00:00

abstract：:Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and conventional standard methods were compared for time to pathogen identification and impact on clinical outcomes in peritoneal dialysis-related peritonitis patients. The MALDI-TOF MS method identified the causative microorga...

journal_title：Journal of clinical microbiology

authors： Lin WH,Hwang JC,Tseng CC,Chang YT,Wu AB,Yan JJ,Wu JJ,Wang MC

更新日期：2016-05-01 00:00:00

abstract：:Transmissible spongiform encephalopathy strains can be differentiated by their behavior in bioassays and by molecular analyses of the disease-associated prion protein (PrP) in a posttranslationally transformed conformation (PrPSc). Until recently, isolates from cases of bovine spongiform encephalopathy (BSE) appeared ...

journal_title：Journal of clinical microbiology

authors： Jacobs JG,Langeveld JP,Biacabe AG,Acutis PL,Polak MP,Gavier-Widen D,Buschmann A,Caramelli M,Casalone C,Mazza M,Groschup M,Erkens JH,Davidse A,van Zijderveld FG,Baron T

更新日期：2007-06-01 00:00:00

abstract：:Evolution of the direct repeat region in Mycobacterium tuberculosis has created unique spoligotype signatures specifically associated with IS6110-defined strain families. Spoligotyping signatures may enable the analysis of the strain population structure in different settings and will enable the rapid identification o...

journal_title：Journal of clinical microbiology

authors： Streicher EM,Victor TC,van der Spuy G,Sola C,Rastogi N,van Helden PD,Warren RM

更新日期：2007-01-01 00:00:00

abstract：:We describe a 2009 H1N1 virus infection with a high viral load in a previously healthy infant who presented with complex febrile seizures and improved on oseltamivir without neurologic sequelae. Febrile seizures may be a complication in young children experiencing infection with high viral loads of 2009 H1N1 influenza...

journal_title：Journal of clinical microbiology

authors： O'Leary MF,Chappell JD,Stratton CW,Cronin RM,Taylor MB,Tang YW

更新日期：2010-10-01 00:00:00

abstract：:Capnocytophaga canimorsus is a dog oral commensal that causes rare but severe infections in humans. C. canimorsus was recently shown to be endowed with a capsular polysaccharide implicated in resistance to the innate immune system of the host. Here, we developed the first C. canimorsus capsular serotyping scheme. We d...

journal_title：Journal of clinical microbiology

authors： Hess E,Renzi F,Koudad D,Dol M,Cornelis GR

更新日期：2017-06-01 00:00:00

abstract：:Rotavirus serotype G9 is recognized as the most widespread of the emerging serotypes, emerging since 1996 as a frequent cause of severe acute gastroenteritis in children from many countries covering all continents of the world. This study characterized serotype G9 strains collected in three widely separated Australian...

journal_title：Journal of clinical microbiology

authors： Kirkwood C,Bogdanovic-Sakran N,Palombo E,Masendycz P,Bugg H,Barnes G,Bishop R

更新日期：2003-08-01 00:00:00

abstract：:The elimination of leprosy continues to be a challenge, with the disease remaining endemic in several countries. India accounts for the highest number of cases, and the identification of child cases indicates recent transmission. Genetic markers, like variable-number tandem repeats (VNTRs) and single-nucleotide polymo...

journal_title：Journal of clinical microbiology

authors： Kuruwa S,Vissa V,Mistry N

更新日期：2012-04-01 00:00:00

abstract：:Recurrence of osteomyelitis by the same bacterial strain is well known. We report three patients with a second episode of osteomyelitis at the same site caused by different strains of bacteria from the original. Formerly infected and altered bone surface might present a region of diminished resistance for a new infect...

journal_title：Journal of clinical microbiology

authors： Uçkay I,Assal M,Legout L,Rohner P,Stern R,Lew D,Hoffmeyer P,Bernard L

更新日期：2006-03-01 00:00:00

abstract：:Serratia ficaria was first described in 1979 as part of the fig tree ecosystem (P.A.D. Grimont, F. Grimont, and M. P. Starr, Curr. Microbiol. 2:277-282, 1979). Since then, it has been isolated from clinical specimens from a few human patients (C. Bollet, J. Freney, P. de Micco, F. Grimont, and P.A.D. Grimont, Méd. Mal...

journal_title：Journal of clinical microbiology

authors： Darbas H,Jean-Pierre H,Paillisson J

更新日期：1994-09-01 00:00:00

abstract：:A total of 1,335 Escherichia coli strains isolated from patients with diarrhea were submitted to the Serény test. Of these strains, 1,035 were also submitted to slide agglutination test, using antisera for invasive E. coli serogroups. Of 325 isolates that were lysine decarboxylase negative, 138 reacted with one of the...

journal_title：Journal of clinical microbiology

authors： Toledo MR,Trabulsi LR

更新日期：1983-03-01 00:00:00

abstract：:Rotaviruses were detected by counterimmunoelectro-osmophoresis in the feces of 16 (13.8%) of 116 infants and young children with gastroenteritis during a 5-month period (September 1979 through January 1980) in Ife, Nigeria. The rate of rotavirus detection varied inversely with relative humidity and was highest in Dece...

journal_title：Journal of clinical microbiology

authors： Paul MO,Erinle EA

更新日期：1982-02-01 00:00:00

abstract：:For a positive coagulase test with certain isolates of Staphylococcus aureus, inoculation of 0.5 ml of coagulase plasma with at least two colonies and 24 h of incubation at 35 to 37 degrees C may be required. S. aureus does not multiply in coagulase plasma. There is also lot-to-lot variation in the sensitivity of coag...

journal_title：Journal of clinical microbiology

authors： Selepak ST,Witebsky FG

更新日期：1985-11-01 00:00:00

abstract：:Infections due to biochemically typical Yersinia enterocolitica usually present as gastroenteritis, mesenteric lymphadenitis, terminal ileitis, and septicemia often with visceral abscesses. In these instances, the isolates have been biochemically typical and of well-established serotypes, namely 0:3 or 0:9 and, in the...

journal_title：Journal of clinical microbiology

authors： Bottone EJ

更新日期：1978-06-01 00:00:00

abstract：:Hafnia alvei strains which possess the attachment-effacement gene (eaeA) may have clinical importance as new diarrhea-causing pathogens and should therefore be differentiated from other H. alvei strains. We characterized diarrheal H. alvei strains, which were positive in the PCR test for the eaeA gene, using biochemic...

journal_title：Journal of clinical microbiology

authors： Ridell J,Siitonen A,Paulin L,Lindroos O,Korkeala H,Albert MJ

更新日期：1995-09-01 00:00:00

abstract：:Mycobacterium tuberculosis that is resistant to both isoniazid (INH) and rifampin (RIF) is spreading. It has become a public health problem in part because the standard culture methods used to determine the appropriate treatment regimen for patients often take months following the presumptive diagnosis of tuberculosis...

journal_title：Journal of clinical microbiology

authors： Massire C,Ivy CA,Lovari R,Kurepina N,Li H,Blyn LB,Hofstadler SA,Khechinashvili G,Stratton CW,Sampath R,Tang YW,Ecker DJ,Kreiswirth BN

更新日期：2011-03-01 00:00:00

abstract：:Routine anaerobic subcultures of macroscopically negative blood culture bottles, performed within 1 day of receipt of the culture and after 5 days of incubation, were evaluated. Anaerobes were recovered from 207 (12.3%) of the total 1,688 positive cultures and, of these, 154 were only detected macroscopically, 11 only...

journal_title：Journal of clinical microbiology

authors： Murray PR,Sondag JE

更新日期：1978-10-01 00:00:00

abstract：:Haemophilus influenzae type e biotype 4 was isolated from a single antemortem blood culture obtained from a 60-year-old, white male with abdominal carcinomatosis. ...

journal_title：Journal of clinical microbiology

authors： Cawthern TH,Marraro RV,McCleskey FK

更新日期：1978-01-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay (ELISA) for blastomycosis was evaluated with 65 human sera from culturally or histologically proven cases of blastomycosis, 53 sera from humans with heterologous infections, and 115 sera from apparently normal people. The diagnostic value of the ELISA was compared with that of the ...

journal_title：Journal of clinical microbiology

authors： Turner S,Kaufman L,Jalbert M

更新日期：1986-02-01 00:00:00

abstract：:Serial cerebrospinal fluid samples were obtained from 14 multiple sclerosis patients over a period of several months and were tested under code for Inoue-Melnick virus. In five of the positive patients, virus was present in 28 of 32 specimens collected over a period of 2 to 5 months. Four patients, from whom a total o...

journal_title：Journal of clinical microbiology

authors： Melnick JL,Seidel E,Wang SS,Muchinik G,Rashti EJ,Jacobs LD,Freeman AI,O'Malley J

更新日期：1985-10-01 00:00:00

abstract：:Tests commonly used for routine determination of anti-Toxoplasma gondii immunoglobulin G (IgG) antibodies show a high level of consistency. However, considerable variations between commercial screening tests are still observed in detecting antibodies present at low concentrations, leading to a number of discrepant and...

journal_title：Journal of clinical microbiology

authors： Franck J,Garin YJ,Dumon H

更新日期：2008-07-01 00:00:00

abstract：:We undertook a study to evaluate Streck tissue fixative (STF) as a substitute for formalin and polyvinyl alcohol (PVA) in fecal preservation. A comparison of formalin, PVA, (mercuric chloride based), and STF was done by aliquoting fecal samples into each fixative. Stool specimens were collected in Haiti, and parasites...

journal_title：Journal of clinical microbiology

authors： Nace EK,Steurer FJ,Eberhard ML

更新日期：1999-12-01 00:00:00

abstract：:Four human coronaviruses (HCoV-229E, HCoV-HKU1, HCoV-NL63, and HCoV-OC43) are associated with a range of respiratory outcomes, including bronchiolitis and pneumonia. Their epidemiologies and clinical characteristics are poorly described and are often reliant on case reports. To address these problems, we conducted a l...

journal_title：Journal of clinical microbiology

authors： Gaunt ER,Hardie A,Claas EC,Simmonds P,Templeton KE

更新日期：2010-08-01 00:00:00

abstract：:A biotyping scheme using improved media and methods for the detection of hippurate hydrolysis, rapid H2S production, and DNA hydrolysis was applied to 1,826 cultures of Campylobacter jejuni, Campylobacter coli and "Campylobacter laridis" isolates from human and nonhuman sources. Four biotypes were identified among C. ...

journal_title：Journal of clinical microbiology

authors： Lior H

更新日期：1984-10-01 00:00:00

abstract：:The detection of class A serine-carbapenemases among species of Enterobacteriaceae remains a challenging issue. Methods of identification for routine use in clinical microbiology laboratories have not been standardized to date. We developed a novel screening methodology suitable for countries with high basal levels of...

journal_title：Journal of clinical microbiology

authors： Pasteran F,Mendez T,Guerriero L,Rapoport M,Corso A

更新日期：2009-06-01 00:00:00

abstract：:Hepatitis C virus (HCV)-positive sera from 106 chronically infected patients which had previously been genotyped were characterized by serotyping. Genotypes were determined by a first-generation line probe assay (INNO-LiPA HCV) and by sequence analyses of the core, core-E1, and NS5B regions. HCV serotypes were determi...

journal_title：Journal of clinical microbiology

authors： van Doorn LJ,Kleter B,Pike I,Quint W

更新日期：1996-07-01 00:00:00

abstract：:tRNA intergenic spacer PCR (tDNA-PCR) was evaluated for its usefulness in the differentiation of enterococcal species of human and animal origin. This technique was carried out for 124 strains belonging to 17 enterococcal species and generated DNA fragments, which were separated by capillary electrophoresis. tDNA-PCR ...

journal_title：Journal of clinical microbiology

authors： Baele M,Baele P,Vaneechoutte M,Storms V,Butaye P,Devriese LA,Verschraegen G,Gillis M,Haesebrouck F

更新日期：2000-11-01 00:00:00

abstract：:The first case of cavitary pulmonary disease caused by Purpureocillium lilacinum is described. The isolate showed atypical microscopic characteristics similar to Acremonium and Fusarium spp., which necessitated molecular identification by sequencing of multiple conserved loci. The patient responded to voriconazole, re...

journal_title：Journal of clinical microbiology

authors： Khan Z,Ahmad S,Al-Ghimlas F,Al-Mutairi S,Joseph L,Chandy R,Sutton DA,Guarro J

更新日期：2012-05-01 00:00:00