Atypical Yersinia enterocolitica: clinical and epidemiological parameters.

abstract：:Maternal prenatal screening for group B streptococci (GBS) followed by offering of intrapartum chemoprophylaxis to carriers is one of the strategies used to reduce the incidence of neonatal early-onset GBS infections. Culturing of vaginal and anorectal swab specimens in selective broth is the screening procedure recom...

journal_title：Journal of clinical microbiology

authors： Hansen SM,Sørensen UB

更新日期：2003-04-01 00:00:00

abstract：:Brevibacterium sp. was isolated from the blood of an acutely ill 18-year-old female with AIDS. The isolate was identified as Brevibacterium casei by use of carbohydrate assimilation tests. Treatment was successful with intravenously administered ciprofloxacin. To our knowledge, this is the first report of sepsis cause...

journal_title：Journal of clinical microbiology

authors： Brazzola P,Zbinden R,Rudin C,Schaad UB,Heininger U

更新日期：2000-09-01 00:00:00

abstract：:We tested 121 pneumococcal serogroup 6 isolates (including 30 serotype 6C and 24 serotype 6D isolates) by serotype-specific PCR and the Quellung reaction, using "old" and "new" pool B, factor 6b, and new factor 6d antisera. In combination with group B and other factor antisera, factor 6d antiserum can reliably identif...

journal_title：Journal of clinical microbiology

authors： Oftadeh S,Satzke C,Gilbert GL

更新日期：2010-09-01 00:00:00

abstract：:Two Hoffmann-La Roche products were evaluated in parallel for their ability to identify 321 strains of the family Enterobacteriaceae. The first product was the well-established Enterotube II, which correctly identified 90% of the isolates after 24 h of incubation. The second was the ID-E rotor, originally designed for...

journal_title：Journal of clinical microbiology

authors： Holmes B

更新日期：1989-05-01 00:00:00

abstract：:We compared the results of Gram staining and culture of cerebrospinal fluid to results obtained with a rapid PCR assay for the diagnosis of meningococcal meningitis in 281 cases of suspected bacterial meningitis. PCR had a sensitivity of 97% compared to a sensitivity of 55% for culture, and the PCR specificity was 99....

journal_title：Journal of clinical microbiology

authors： Richardson DC,Louie L,Louie M,Simor AE

更新日期：2003-08-01 00:00:00

abstract：:By using the computer-assisted Dendron system to analyze the patterns of Southern blots probed with the repetitive sequence Ca3, we have compared oral isolates of Candida spp. from a group of 11 nonhospitalized patients with AIDS suffering from recurrent episodes of oral thrush in Leicester, England, with oral isolate...

journal_title：Journal of clinical microbiology

authors： Schmid J,Odds FC,Wiselka MJ,Nicholson KG,Soll DR

更新日期：1992-04-01 00:00:00

abstract：:A total of 145 clinical strains of Candida species were tested by the Clinical and Laboratory Standards Institute M27-A3 methodology to determine if replacing water with dimethyl sulfoxide as the solvent for fluconazole and flucytosine impacted the in vitro potency. No significant differences in MIC values were observ...

journal_title：Journal of clinical microbiology

authors： Fothergill AW,Sanders C,Wiederhold NP

更新日期：2013-06-01 00:00:00

abstract：:In a prospective study of 16 patients with Legionnaires disease confirmed by cultural isolation of Legionella pneumophila from the respiratory tract, 38% (6 of 16) had positive blood cultures. Daily subcultures were made onto buffered charcoal-yeast extract plates from 6B aerobic and 7C anaerobic BACTEC blood culture ...

journal_title：Journal of clinical microbiology

authors： Rihs JD,Yu VL,Zuravleff JJ,Goetz A,Muder RR

更新日期：1985-09-01 00:00:00

abstract：:Endotoxin concentrations were determined in middle-ear effusions (MEEs) from 89 children with chronic otitis media by using the Limulu's amoebocyte lysate assay. Mean concentrations of endotoxin in Haemophilus influenzae-positive and Streptococcus pneumoniae-positive MEEs were 157 and 21.8 ng/ml, respectively, and wer...

journal_title：Journal of clinical microbiology

authors： DeMaria TF,Prior RB,Briggs BR,Lim DJ,Birck HG

更新日期：1984-07-01 00:00:00

abstract：:Serratia plymuthica was isolated from the blood and from a central venous catheter tip in a 54-year-old man with alcoholic cirrhosis and clinical signs of sepsis. This was the seventh reported isolate of S. plymuthica from a clinical specimen and the first in which the organism was clearly a significant pathogen. ...

journal_title：Journal of clinical microbiology

authors： Horowitz HW,Nadelman RB,Van Horn KG,Weekes SE,Goyburu L,Wormser GP

更新日期：1987-08-01 00:00:00

abstract：:The food-borne pathogen Listeria monocytogenes is genetically heterogeneous. Although some clonal groups have been implicated in multiple outbreaks, there is currently no consensus on how "epidemic clones" should be defined. The objectives of this work were to compare the patterns of sequence diversity on two sets of ...

journal_title：Journal of clinical microbiology

authors： Cantinelli T,Chenal-Francisque V,Diancourt L,Frezal L,Leclercq A,Wirth T,Lecuit M,Brisse S

更新日期：2013-11-01 00:00:00

abstract：:The hexagonal surface structure believed to be characteristic for influenza C virus was not initially observed for a newly isolated virus of this type. After a few additional egg passages of the virus, however, the hexagonal structure could be readily seen on many virions. ...

journal_title：Journal of clinical microbiology

authors： Martin ML,Palmer EL,Kendal AP

更新日期：1977-07-01 00:00:00

abstract：:Many laboratories are unable to perform colistin susceptibility testing. Diffusion-based antimicrobial susceptibility testing methods are not recommended, and not all laboratories have the capacity to perform broth microdilution (BMD). Using a multistep tiered approach, we investigated whether the adapted use of the M...

journal_title：Journal of clinical microbiology

authors： Lutgring JD,Kim A,Campbell D,Karlsson M,Brown AC,Burd EM

更新日期：2019-04-26 00:00:00

abstract：:Antibiogram typing of methicillin-resistant Staphylococcus aureus with selected antibiotics was evaluated as a primary epidemiological typing tool and compared with ribotyping. Antibiograms were derived with the Kirby-Bauer disk diffusion method by using erythromycin, clindamycin, cotrimoxazole, gentamicin, and ciprof...

journal_title：Journal of clinical microbiology

authors： Blanc DS,Lugeon C,Wenger A,Siegrist HH,Francioli P

更新日期：1994-10-01 00:00:00

abstract：:A new principle that uses anti-immunoglobulin M-coated polystyrene microtiter plates for the detection of immunoglobulin M antibodies against Toxoplasma gondii by an enzyme-linked immunosorbent assay was developed. ...

journal_title：Journal of clinical microbiology

authors： Duermeyer W,Wielaard F,van Gruijthuijsen H,Swinkels J

更新日期：1980-12-01 00:00:00

abstract：:We describe a 2009 H1N1 virus infection with a high viral load in a previously healthy infant who presented with complex febrile seizures and improved on oseltamivir without neurologic sequelae. Febrile seizures may be a complication in young children experiencing infection with high viral loads of 2009 H1N1 influenza...

journal_title：Journal of clinical microbiology

authors： O'Leary MF,Chappell JD,Stratton CW,Cronin RM,Taylor MB,Tang YW

更新日期：2010-10-01 00:00:00

abstract：:A method is presented for preparing smears for proficiency testing and quality control in acid-fast microscopy. The work was prompted by the increased demand for acid-fast bacilli positive smears with characteristic microscopic appearance and among-smear uniformity. ...

journal_title：Journal of clinical microbiology

authors： Smithwick RW,Stratigos CB

更新日期：1978-07-01 00:00:00

abstract：:Crude extracts of Dolichos biflorus can be coupled to polystyrene particles to yield an agglutination reagent for the detection of group C streptococcal antigen extracts. The reagent is relatively inexpensive and simple to prepare and can be employed for the definitive identification of this beta-hemolytic streptococc...

journal_title：Journal of clinical microbiology

authors： Slifkin M,Gil GM

更新日期：1984-01-01 00:00:00

abstract：:Campylobacter jejuni from sporadic cases and outbreaks of gastroenteritis were serotyped on the basis of heat-extracted soluble thermostable antigens identified with the use of the passive hemagglutination technique. A total of 168 isolates were separated into 45 different types. The largest proportion of the isolates...

journal_title：Journal of clinical microbiology

authors： McMyne PM,Penner JL,Mathias RG,Black WA,Hennessy JN

更新日期：1982-08-01 00:00:00

abstract：:A noncompetitive enzyme-linked immunosorbent assay that enables the quantitation of toxic shock syndrome toxin 1 (TSST-1) to as little as 30 pg/ml and the detection of TSST-1 to 10 pg/ml in phosphate-buffered saline including 33% human serum or plasma was developed. It takes only 3 h to complete this assay after plate...

journal_title：Journal of clinical microbiology

authors： Miwa K,Fukuyama M,Kunitomo T,Igarashi H

更新日期：1994-02-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay for the measurement of antibodies directed against cell surface antigens of Vibrio cholerae (CSA ELISA) was developed. NaN3-killed whole cells of V. cholerae, adsorbed to polystyrene tubes, were used as immobilized antigens. The assay was capable of detecting antibodies directed ag...

journal_title：Journal of clinical microbiology

authors： Cryz SJ Jr,Fürer E,Germanier R

更新日期：1982-07-01 00:00:00

abstract：:We developed a sensitive and specific sodB-based quantitative PCR assay to detect Ehrlichia spp. The assay's limit of detection was 5 copies/reaction, and it did not amplify nonspecific DNA. Compared with a 16S rRNA gene PCR target, the sodB target may offer an improved molecular diagnostic assay to detect Ehrlichia s...

journal_title：Journal of clinical microbiology

authors： Qurollo BA,Riggins D,Comyn A,Zewde MT,Breitschwerdt EB

更新日期：2014-11-01 00:00:00

abstract：:Oligonucleotide chips targeting the bacterial internal transcribed spacer region (ITS) of the 16S-23S rRNA gene, which contains genus- and species-specific regions, were developed and evaluated. Forty-three sequences were designed consisting of 1 universal, 3 Gram stain-specific, 9 genus-specific, and 30 species-speci...

journal_title：Journal of clinical microbiology

authors： Kim CM,Song ES,Jang HJ,Kim HJ,Lee S,Shin JH,Kim SJ,Jeong SH,Jeong J,Koh K,Choi GE,Lee EY,Chang CL

更新日期：2010-05-01 00:00:00

abstract：:The 37-kDa protein (P37) of Borrelia burgdorferi is an antigen that elicits an early immunoglobulin M (IgM) antibody response in Lyme disease patients. The P37 gene was cloned from a B. burgdorferi genomic library by screening with antibody from a Lyme disease patient who had developed a prominent humoral response to ...

journal_title：Journal of clinical microbiology

authors： Gilmore RD Jr,Murphree RL,James AM,Sullivan SA,Johnson BJ

更新日期：1999-03-01 00:00:00

abstract：:Lipooligosaccharide configurations were predicted in nontypeable Haemophilus influenzae isolates based on the presence of seven oligosaccharide extension-initiating genes (or alleles). Predicted configurations with 2 to 3 oligosaccharide extensions were more prevalent among middle ear than throat strains. In addition,...

journal_title：Journal of clinical microbiology

authors： McCrea KW,Xie J,Daniel D,Ulrich-Lewis JT,Zhang L

更新日期：2014-07-01 00:00:00

abstract：:A toxic shock syndrome toxin 1 (TSST-1) antibody-binding protein produced by an ovine-associated strain of Staphylococcus aureus was examined. The protein showed total identity to TSST-1 by immunodiffusion analysis. Western blots (immunoblots) of proteins separated by isoelectric focusing revealed that the TSST-1 anti...

journal_title：Journal of clinical microbiology

authors： Ho G,Campbell WH,Carlson E

更新日期：1989-01-01 00:00:00

abstract：:Sequencing-based pathogen identification directly from clinical specimens requires time-consuming interpretation, especially with mixed chromatograms when multiple microorganisms are detected. We assessed RipSeq Mixed software for human papillomavirus (HPV) genotyping by comparison to the linear array HPV genotyping a...

journal_title：Journal of clinical microbiology

authors： Tardif KD,Simmon KE,Kommedal O,Pyne MT,Schlaberg R

更新日期：2013-04-01 00:00:00

abstract：:Unbiased nontargeted metagenomic RNA sequencing (UMERS) has the advantage to detect known as well as unknown pathogens and, thus, can significantly improve the detection of viral, bacterial, parasitic, and fungal sequences in public health settings. In particular, conventional diagnostic methods successfully identify ...

journal_title：Journal of clinical microbiology

authors： Fischer N,Indenbirken D,Meyer T,Lütgehetmann M,Lellek H,Spohn M,Aepfelbacher M,Alawi M,Grundhoff A

更新日期：2015-07-01 00:00:00

abstract：:Monoclonal antibodies to K88ac and K88ab were used in enzyme-linked immunosorbent assays on Escherichia coli cultures known to produce K88 pili. A total of 415 K88-positive E. coli isolates from nine states were all found to be the K88ac variant. The cultures tested were isolated during the years 1976 to 1985. ...

journal_title：Journal of clinical microbiology

authors： Westerman RB,Mills KW,Phillips RM,Fortner GW,Greenwood JM

更新日期：1988-01-01 00:00:00

abstract：:AmpC regulatory genes in 21 ceftazidime-resistant clinical isolates of Enterobacter cloacae (MICs of > or = 16 microg/ml) were characterized. All isolates exhibited AmpC overproduction due to AmpD mutation. Additionally, we found two AmpR mutants among the isolates. This is the first report of chromosomal ampR mutatio...

journal_title：Journal of clinical microbiology

authors： Kaneko K,Okamoto R,Nakano R,Kawakami S,Inoue M

更新日期：2005-06-01 00:00:00