Development and evaluation of oligonucleotide chip based on the 16S-23S rRNA gene spacer region for detection of pathogenic microorganisms associated with sepsis.

abstract：:An 840-bp fragment of the 18S rRNA gene was used to identify Cryptosporidium spp. recovered from human immunodeficiency virus (HIV)-infected and -uninfected patients from Kenya, Malawi, Brazil, the United Kingdom, and Vietnam. Initial identification was by Ziehl-Neelsen acid-fast staining. Confirmation was by nested P...

journal_title：Journal of clinical microbiology

authors： Gatei W,Greensill J,Ashford RW,Cuevas LE,Parry CM,Cunliffe NA,Beeching NJ,Hart CA

更新日期：2003-04-01 00:00:00

abstract：:We investigated the performance of a double-antigen sandwich recombinant enzyme immunoassay (EIA; Abbott Laboratories, North Chicago, Ill.) and compared it with that of a synthetic-peptide-based EIA (Biochem Immunosystems, Montreal, Quebec, Canada) for the detection of human immunodeficiency type 1 (HIV-1) and HIV-2 a...

journal_title：Journal of clinical microbiology

authors： Galli RA,Castriciano S,Fearon M,Major C,Choi KW,Mahony J,Chernesky M

更新日期：1996-04-01 00:00:00

abstract：:A 1-year prospective study was carried out in two large urban centers of São Paulo State, Brazil, to determine the prevalences and roles of the different Escherichia coli pathotypes in children less than 5 years of age with diarrhea presenting to the emergency rooms of public hospitals or visiting private pediatrician...

journal_title：Journal of clinical microbiology

authors： Araujo JM,Tabarelli GF,Aranda KR,Fabbricotti SH,Fagundes-Neto U,Mendes CM,Scaletsky IC

更新日期：2007-10-01 00:00:00

abstract：:Pyridine-2-azo-p-dimethylanaline cephalosporin (PADAC), a chromogenic reagent which is purple and changes to yellow upon cleavage of its beta-lactam ring, was evaluated in comparison with other chromogenic cephalosporins. PADAC exhibited little antimicrobial activity against gram-negative bacteria, but did have good a...

journal_title：Journal of clinical microbiology

authors： Jones RN,Wilson HW,Novick WJ Jr

更新日期：1982-04-01 00:00:00

abstract：:The slightly revised, current scheme of 20 flagellar (H) antigens of Serratia marcesens was examined. The seven new H antigens were demonstrated to be antigenically distinct as determined with Le Minor's H-immobilization test. The H-immobilization antibodies of rabbit anti-H immune sera proved resistant to treatment w...

journal_title：Journal of clinical microbiology

authors： Traub WH,Fukushima PI

更新日期：1979-07-01 00:00:00

abstract：:Quantitative real-time PCR for the detection of respiratory syncytial virus (RSV) RNA is increasingly used to study the causal role of RSV in lower airway disease. The objective of our study was to evaluate variations in RSV RNA loads at different steps in the RNA quantification process: (i) variation in RSV RNA load ...

journal_title：Journal of clinical microbiology

authors： van de Pol AC,Wolfs TF,van Loon AM,Tacke CE,Viveen MC,Jansen NJ,Kimpen JL,Rossen JW,Coenjaerts FE

更新日期：2010-10-01 00:00:00

abstract：:Blastomyces dermatitidis and Histoplasma capsulatum are dimorphic fungi that often cause self-limited respiratory infections. However, they may also cause severe disseminated disease, depending on the level of the exposure to the organism and the host immune status. In addition, patients with infections caused by thes...

journal_title：Journal of clinical microbiology

authors： Babady NE,Buckwalter SP,Hall L,Le Febre KM,Binnicker MJ,Wengenack NL

更新日期：2011-09-01 00:00:00

abstract：:A dot immunobinding assay was used to detect antibodies to measles virus, mumps virus, and rubella virus antigens. Filter paper soaked with serum or whole blood was directly applied to the antigen-coated nitrocellulose sheets. The test was easy to perform, and its results agreed very well with those obtained by standa...

journal_title：Journal of clinical microbiology

authors： Condorelli F,Ziegler T

更新日期：1993-03-01 00:00:00

abstract：:Campylobacter rectus was isolated under routine anaerobic conditions (no additional hydrogen gas in the atmosphere) from an oral, nonperiodontal abscess from a patient with gastroesophageal adenocarcinoma. We report the first case of a palate abscess caused by C. rectus and review the literature and atmospheric requir...

journal_title：Journal of clinical microbiology

authors： Mahlen SD,Clarridge JE 3rd

更新日期：2009-03-01 00:00:00

abstract：:Clinical isolates of Haemophilus influenzae were assessed for the presence of fucK, hap, and sodC by hybridization with gene-specific probes, and isolates diverging from the expected H. influenzae genotype were characterized by phenotype and 16S rRNA gene sequencing. Two of 480 isolates were finally classified as vari...

journal_title：Journal of clinical microbiology

authors： Nørskov-Lauritsen N

更新日期：2009-08-01 00:00:00

abstract：:For prevention and control of gonorrhea, an objective, highly discriminating, and reproducible molecular epidemiological characterization of Neisseria gonorrhoeae is essential. In the present study, in pursuance of providing such qualities, pyrosequencing technology, a fast real-time DNA sequence analysis, was applied...

journal_title：Journal of clinical microbiology

authors： Unemo M,Olcén P,Jonasson J,Fredlund H

更新日期：2004-07-01 00:00:00

abstract：:Neisseria gonorrhoeae is a common bacterial sexually transmitted infection (STI). Currently, there are limited data on the bacterial load in both men and women and on both genital and extragenital sites. Therefore, we quantified N. gonorrhoeae bacterial loads in a large population of women, heterosexual men, and men w...

journal_title：Journal of clinical microbiology

authors： van der Veer BMJW,Hoebe CJPA,Dukers-Muijrers NHTM,van Alphen LB,Wolffs PFG

更新日期：2020-10-21 00:00:00

abstract：:We describe two cases in Brazil of human subcutaneous infections due to Phaeoacremonium spp. The first case was caused by Phaeoacremonium aleophilum. The patient presented with a unique fistulized nodule on the left ankle. The fungus was detected by direct microscopic examination and was isolated repeatedly from mater...

journal_title：Journal of clinical microbiology

authors： Guarro J,Alves SH,Gené J,Grazziotin NA,Mazzuco R,Dalmagro C,Capilla J,Zaror L,Mayayo E

更新日期：2003-03-01 00:00:00

abstract：:We have used a polymerase chain reaction (PCR) to examine cervical cancer biopsy specimens and pelvic lymph nodes for the presence of human papillomavirus type 16 (HPV 16) DNA. Of the 75 cervical specimens tested, 36 (48%) were positive for HPV 16 in the PCR. A total of 65 pelvic lymph nodes removed during radical sur...

journal_title：Journal of clinical microbiology

authors： Czeglédy J,Póka R,Veress G,Gergely L

更新日期：1992-01-01 00:00:00

abstract：:Nocardia identification required laborious and time-consuming phenotypic and chemotaxonomic methods until molecular methods were developed in the mid-1990s. Here we reassessed the capacity of PCR-restriction enzyme pattern analysis (PRA) of the hsp65 gene to differentiate Nocardia species, including 36 new species. Ou...

journal_title：Journal of clinical microbiology

authors： Rodríguez-Nava V,Couble A,Devulder G,Flandrois JP,Boiron P,Laurent F

更新日期：2006-02-01 00:00:00

abstract：:Of 90 clinical isolates of Cryptococcus neoformans studied, 3 were determined to be serotype B/C. The patients from whom these B/C isolates were obtained were identified as never having lived in or visited the areas associated with B/C serotypes. This finding suggests a broader geographic distribution of this serotype...

journal_title：Journal of clinical microbiology

authors： Fromtling RA,Shadomy S,Shadomy HJ,Dismukes WE

更新日期：1982-08-01 00:00:00

abstract：:Eight strains of a new intermediate adenovirus were isolated in the course of an investigation of conjunctivitis in Hiroshima City, Japan. The strain was first isolated in July 1986. All eight strains were isolated from conjunctival swab samples from patients with conjunctivitis, mainly epidemic keratoconjunctivitis. ...

journal_title：Journal of clinical microbiology

authors： Noda M,Miyamoto Y,Ikeda Y,Matsuishi T,Ogino T

更新日期：1991-07-01 00:00:00

abstract：:We have introduced a time-resolved fluorometry (TRF)-based microwell hybridization assay for PCR products in detection of herpes simplex virus (HSV) in cerebrospinal fluid (CSF) specimens. TRF is a sensitive nonradioactive detection technique which involves the use of lanthanide chelates as fluorescent labels. We used...

journal_title：Journal of clinical microbiology

authors： Hukkanen V,Rehn T,Kajander R,Sjöroos M,Waris M

更新日期：2000-09-01 00:00:00

abstract：:A singleplex PCR assay using a single primer pair targeting the putative sugar transporter gene was developed here to distinguish Cryptococcus neoformans var. grubii, Cryptococcus neoformans var. neoformans, and Cryptococcus gattii according to the distinct size of the amplicon. The interspecies and intravarietal hybr...

journal_title：Journal of clinical microbiology

authors： Feng X,Fu X,Ling B,Wang L,Liao W,Yao Z

更新日期：2013-06-01 00:00:00

abstract：:The QuantiFERON-TB Gold Plus (QFT-Plus; Qiagen, Germantown, MD) interferon gamma release assay (IGRA) received FDA clearance in 2017 and will replace the prior version of the assay, the QFT-Gold In-Tube (QFT-GIT). Here, we compared performances of the QFT-Plus assay and the QFT-GIT version in a diverse patient populat...

journal_title：Journal of clinical microbiology

authors： Theel ES,Hilgart H,Breen-Lyles M,McCoy K,Flury R,Breeher LE,Wilson J,Sia IG,Whitaker JA,Clain J,Aksamit TR,Escalante P

更新日期：2018-06-25 00:00:00

abstract：:Genotyping and subtyping of 225 samples with hepatitis C virus (HCV) genotype 1, 2, 3, or 6 infection were done with Versant LiPA 2.0 and Abbott RealTime HCV Genotype (GT) II by using direct sequencing of the NS5B and 5' untranslated regions as the reference standards. The concordance rates were >99.2% for genotypes a...

journal_title：Journal of clinical microbiology

authors： Liu CH,Liang CC,Liu CJ,Lin CL,Su TH,Yang HC,Chen PJ,Chen DS,Kao JH

更新日期：2015-05-01 00:00:00

abstract：:This study compared the abilities of API 20E and BBL Crystal E/NF identification systems to correctly identify human and animal source gram-negative bacilli of known identifications, as provided by the American Type Culture Collection, Rockville, Md., and the Research Diagnostic and Investigative Laboratory, Columbia,...

journal_title：Journal of clinical microbiology

authors： Peele D,Bradfield J,Pryor W,Vore S

更新日期：1997-01-01 00:00:00

abstract：:Few studies have assessed the time to blood culture positivity as a predictor of clinical outcome in bloodstream infections (BSIs). The purpose of this study was to evaluate the time to positivity (TTP) of blood cultures in patients with Staphylococcus aureus BSIs and to assess its impact on clinical outcome. We perfo...

journal_title：Journal of clinical microbiology

authors： Marra AR,Edmond MB,Forbes BA,Wenzel RP,Bearman GM

更新日期：2006-04-01 00:00:00

abstract：:The one-step single-tube betaine-free reverse transcription (RT)-loop-mediated isothermal amplification assay was developed for rapid diagnosis of hepatitis E virus. This assay amplified the target gene in less than 45 min (even as short as 20 min) under isothermal conditions at 63 degrees C, and the sensitivity of th...

journal_title：Journal of clinical microbiology

authors： Lan X,Yang B,Li BY,Yin XP,Li XR,Liu JX

更新日期：2009-07-01 00:00:00

abstract：:We compared the BD MAX enteric bacterial panel (EBP) to culture for the detection of Salmonella, Shigella, Campylobacter, and Shiga toxin-producing enterohemorrhagic Escherichia coli (EHEC) O157 in seeded stool samples. The EBP panel demonstrated superior sensitivity and reliably detected Salmonella, EHEC O157, Shigel...

journal_title：Journal of clinical microbiology

authors： Anderson NW,Buchan BW,Ledeboer NA

更新日期：2014-04-01 00:00:00

abstract：:A case of tick-borne encephalitis (TBE) has not been reported for many years in Japan, although a serological survey of sera from domestic animals suggested the presence of TBE foci in Hokkaido, the northern island of Japan. Studies were conducted to prove the presence of an endemic focus of TBE virus in Japan by mean...

journal_title：Journal of clinical microbiology

authors： Takashima I,Morita K,Chiba M,Hayasaka D,Sato T,Takezawa C,Igarashi A,Kariwa H,Yoshimatsu K,Arikawa J,Hashimoto N

更新日期：1997-08-01 00:00:00

abstract：:In this study, the optimal combination of three commercial glycoprotein G-2 (gG-2)-based herpes simplex virus type 2 (HSV-2) type-specific enzyme-linked immunosorbent assays (Euroimmun anti-HSV-2 immunoglobulin G [IgG] ELISA [Eu2], Gull HSV-2-specific IgG ELISA [Gu2], and Radim HSV-2 IgG ELISA [Ra2]) and one gG-2-base...

journal_title：Journal of clinical microbiology

authors： Eing BR,Lippelt L,Lorentzen EU,Hafezi W,Schlumberger W,Steinhagen K,Kühn JE

更新日期：2002-02-01 00:00:00

abstract：:The establishment of an optimal noninvasive method for diagnosing invasive aspergillosis (IA) is needed to improve the management of this life-threatening infection in patients with hematological disorders, and a number of noninvasive tests for IA that target different fungal components, including galactomannan, (1-->...

journal_title：Journal of clinical microbiology

authors： Kawazu M,Kanda Y,Nannya Y,Aoki K,Kurokawa M,Chiba S,Motokura T,Hirai H,Ogawa S

更新日期：2004-06-01 00:00:00

abstract：:Relative amounts of D-arabinitol (fungal origin) and L-arabinitol (part of normal human metabolism) in urine were determined by gas chromatography and mass spectrometry from 61 hospitalized patients with hematological malignancies. Seventeen neutropenic patients with acute leukemia (with 53 samples) had disseminated y...

journal_title：Journal of clinical microbiology

authors： Lehtonen L,Anttila VJ,Ruutu T,Salonen J,Nikoskelainen J,Eerola E,Ruutu P

更新日期：1996-09-01 00:00:00

abstract：:A monoclonal antibody specific for group D Salmonella antigen 9 was used in an indirect enzyme-linked immunosorbent assay (ELISA) for detecting the antigen in urine specimens collected from patients with clinical typhoid fever in Jakarta, Indonesia. The ELISA had a sensitivity of 95% in identifying patients in whom Sa...

journal_title：Journal of clinical microbiology

authors： Chaicumpa W,Ruangkunaporn Y,Burr D,Chongsa-Nguan M,Echeverria P

更新日期：1992-09-01 00:00:00