Comparative evaluation of the Roche Cobas IDA and Enterotube II systems for identifying members of the family Enterobacteriaceae.

abstract：:We present the first case of severe pneumonia possibly caused by Leptotrichia species with oral bacteria. This was found in a healthy but elderly subject whose bronchoalveolar lavage fluid was analyzed by 16S rRNA gene sequencing analysis. The combination of this method and microscopic observation provided useful info...

journal_title：Journal of clinical microbiology

authors： Kawanami T,Fukuda K,Yatera K,Kido T,Yoshii C,Taniguchi H,Kido M

更新日期：2009-02-01 00:00:00

abstract：:In Brazil, the most common etiological agent of American tegumentary leishmaniasis is Leishmania (Viannia) braziliensis. In general, diagnostic techniques envisage the visualization of the parasite, but that technique has a low sensitivity. The main purpose of the present work was to evaluate the PCR as a routine tool...

journal_title：Journal of clinical microbiology

authors： Pirmez C,da Silva Trajano V,Paes-Oliveira Neto M,da-Cruz AM,Gonçalves-da-Costa SC,Catanho M,Degrave W,Fernandes O

更新日期：1999-06-01 00:00:00

abstract：:Aberrant filamentous forms of Escherichia coli were observed on direct Gram stain of blood cultures from a patient being treated with the beta-lactam antibiotic cephalexin. After the institution of an alternative antibiotic regimen which included a different cell wall-active agent, E. coli of normal morphology was det...

journal_title：Journal of clinical microbiology

authors： Magnussen CR,Hruska JF

更新日期：1980-11-01 00:00:00

abstract：:Blood was cultured in two vacuum bottles containing Columbia broth. Filtered air was admitted to one bottle (aerobic); the unvented bottle was considered anaerobic. Cultures were incubated at 35 C until growth occurred or for at least 7 days. Of 744 organisms isolated, 50% were isolated from both bottles, 30% from the...

journal_title：Journal of clinical microbiology

authors： Blazevic DJ,Stemper JE,Matsen JM

更新日期：1975-02-01 00:00:00

abstract：:In this work, we show that antibodies to baculovirus recombinant DNA polymerase of human cytomegalovirus are present in human sera during natural infection. Specific antibody was found to be of the immunoglobulin G class, produced at low titers only for a short period of time at the beginning of acute infection and no...

journal_title：Journal of clinical microbiology

authors： Landini MP,Lazzarotto T,Ertl PF

更新日期：1993-03-01 00:00:00

abstract：:Nineteen isolates of leptospires recovered from patients during three epidemics that occurred at different places and different times in the Andaman Islands and eight isolates from sporadic cases were characterized using serological and molecular genetic techniques. Group sera and monoclonal antibodies were used for a...

journal_title：Journal of clinical microbiology

authors： Vijayachari P,Ahmed N,Sugunan AP,Ghousunnissa S,Rao KR,Hasnain SE,Sehgal SC

更新日期：2004-08-01 00:00:00

abstract：:Cefaclor is less stable than most cephalosporins in media at 35 degrees C. We demonstrated that the activity of cefaclor in Mueller-Hinton agar diminishes continuously at 4 degrees C, resulting in a loss of two-thirds of the activity within 21 days. We recommend that agar dilution plates for this cephalosporin be prep...

journal_title：Journal of clinical microbiology

authors： Surprenant AM,Preston DA

更新日期：1985-01-01 00:00:00

abstract：:Enterobacteriaceae represent a diverse and medically important family of bacteria that are difficult to identify to the species level using the standard molecular method of 16S rRNA gene sequencing. Prior work has demonstrated the value of dnaJ gene sequence analysis in resolving different members of the family. Howev...

journal_title：Journal of clinical microbiology

authors： McLean K,Rosenthal CA,Sengupta D,Owens J,Cookson BT,Hoffman NG,Salipante SJ

更新日期：2019-10-23 00:00:00

abstract：:Mycobacterium avium subsp. paratuberculosis is the causative agent of Johne's disease (JD) in cattle and may be associated with Crohn's disease (CD) in humans. It is the slowest growing of the cultivable mycobacteria, and culture from clinical, veterinary, food, or environmental specimens can take 4 months or even lon...

journal_title：Journal of clinical microbiology

authors： Imirzalioglu C,Dahmen H,Hain T,Billion A,Kuenne C,Chakraborty T,Domann E

更新日期：2011-05-01 00:00:00

abstract：:Vaginal isolates of Candida albicans from human immunodeficiency virus-positive (HIV+) and HIV- women with or without candidal vaginitis were examined for secretory aspartyl proteinase (Sap) production in vitro and in vivo and for the possible correlation of Sap production with pathology and antimycotic susceptibility...

journal_title：Journal of clinical microbiology

authors： de Bernardis F,Mondello F,Scaravelli G,Pachì A,Girolamo A,Agatensi L,Cassone A

更新日期：1999-05-01 00:00:00

abstract：:In 271 Enterobacter blood culture isolates from 12 hospitals, extended-spectrum beta-lactamase (ESBL) prevalence varied between 0% and 30% per hospital. High prevalence was associated with dissemination, indicating the potential relevance of infection control measures. Screening with cefepime or Vitek 2, followed by a...

journal_title：Journal of clinical microbiology

authors： Stuart JC,Diederen B,Al Naiemi N,Fluit A,Arents N,Thijsen S,Vlaminckx B,Mouton JW,Leverstein-van Hall M

更新日期：2011-07-01 00:00:00

abstract：:Pulsed-field gel electrophoresis (PFGE) of 61 Salmonella enterica serotype Paratyphi C isolates from six countries gave five distinct clusters. Twenty-four isolates from five countries were susceptible to 10 antimicrobials tested and gave similar restriction endonuclease digest patterns of the 38-MDa plasmid. In contr...

journal_title：Journal of clinical microbiology

authors： Kariuki S,Cheesbrough J,Mavridis AK,Hart CA

更新日期：1999-06-01 00:00:00

abstract：:Real-time quantitative PCR (qPCR) assay of sputum or nasopharyngeal specimens has shown promising results in the detection of pneumococcal community-acquired pneumonia (PncCAP). We applied qPCR for the autolysin gene (lytA) and compared sputum and nasopharyngeal swab (NPS) pneumococcal loads in elderly patients with c...

journal_title：Journal of clinical microbiology

authors： Saukkoriipi A,Palmu AA,Pascal T,Verlant V,Hausdorff WP,Jokinen J

更新日期：2017-12-26 00:00:00

abstract：:The cellular fatty acid compositions and ubiquinone contents of 182 Legionella strains representing 23 species were determined by capillary gas-liquid chromatography and reverse-phase high-performance liquid chromatography, respectively. Except for the type strain of Legionella erythra (ATCC 35303T), all Legionella sp...

journal_title：Journal of clinical microbiology

authors： Lambert MA,Moss CW

更新日期：1989-03-01 00:00:00

abstract：:Coagulase-negative staphylococci obtained from patients with continuous ambulatory peritoneal dialysis-related peritonitis were cultured in nutrient broth in an atmosphere of air containing 5% carbon dioxide (reflecting the CO2 tension found in freshly used dialysate). Significant differences were observed between the...

journal_title：Journal of clinical microbiology

authors： Denyer SP,Davies MC,Evans JA,Finch RG,Smith DG,Wilcox MH,Williams P

更新日期：1990-08-01 00:00:00

abstract：:Pneumococcal colonization was studied in 19 children monitored from birth through the age of 2 years. For this purpose, pneumococcal isolates were characterized by capsular typing, restriction fragment end labeling (RFEL), and penicillin-binding protein (PBP) genotyping. Fifty-eight isolates were collected and were fo...

journal_title：Journal of clinical microbiology

authors： Sluijter M,Faden H,de Groot R,Lemmens N,Goessens WH,van Belkum A,Hermans PW

更新日期：1998-08-01 00:00:00

abstract：:We report a case of cerebral phaeohyphomycosis in a 36-year-old male caused by the neurotropic fungus Ramichloridium obovoideum (Matushima) de Hoog 1977 (Ramichloridium mackenziei Campbell et Al-Hedaithy 1993). This man resided in the Middle East, where the fungus appears to be endemic and, possibly, geographically re...

journal_title：Journal of clinical microbiology

authors： Sutton DA,Slifkin M,Yakulis R,Rinaldi MG

更新日期：1998-03-01 00:00:00

abstract：:The FUNGITEST method (Sanofi Diagnostics Pasteur, Paris, France) is a microplate-based procedure for the breakpoint testing of six antifungal agents (amphotericin B, flucytosine, fluconazole, itraconazole, ketoconazole, and miconazole). We compared the FUNGITEST method with a broth microdilution test, performed accord...

journal_title：Journal of clinical microbiology

authors： Davey KG,Holmes AD,Johnson EM,Szekely A,Warnock DW

更新日期：1998-04-01 00:00:00

abstract：:We developed a group B streptococcus multiplex PCR assay which allows, by direct analysis of the amplicon size, determination of the surface protein antigen genes of alpha-C protein, epsilon protein, Rib, Alp2, Alp3, and Alp4. The multiplex PCR assay offers a rapid and simple method of subtyping Streptococcus agalacti...

journal_title：Journal of clinical microbiology

authors： Creti R,Fabretti F,Orefici G,von Hunolstein C

更新日期：2004-03-01 00:00:00

abstract：:A urease-based enzyme-linked immunosorbent assay (ELISA) has been developed for the detection of reagin antibodies in serum. Visuwell Reagin (ADI Diagnostics Inc., Rexdale, Ontario, Canada) is a non-treponemal screening test for the serodiagnosis of syphilis which has the benefits of large batch testing, automatabilit...

journal_title：Journal of clinical microbiology

authors： White TJ,Fuller SA

更新日期：1989-10-01 00:00:00

abstract：:A novel PCR restriction analysis method using the RNA polymerase beta-subunit- coding gene (rpoB) was employed to both detect and identify Helicobacter pylori in biopsy specimens and culture isolates. The rpoB DNAs (458 bp) were specifically amplified by PCR with the Helicobacter-specific primers (HF and HR). Based on...

journal_title：Journal of clinical microbiology

authors： Lim CY,Lee KH,Cho MJ,Chang MW,Kim SY,Myong NH,Lee WK,Rhee KH,Kook YH

更新日期：2003-07-01 00:00:00

abstract：:Paracoccidioides brasiliensis isolates from 10 nine-banded armadillos (Dasypus novemcinctus) were comparable with 19 clinical isolates by sequence analysis of the PbGP43 gene and ribosomal internal transcribed spacer 1 (ITS1) and ITS2 and by random amplified polymorphic DNA. In this original ITS study, eight isolates ...

journal_title：Journal of clinical microbiology

authors： Hebeler-Barbosa F,Morais FV,Montenegro MR,Kuramae EE,Montes B,McEwen JG,Bagagli E,Puccia R

更新日期：2003-12-01 00:00:00

abstract：:Salmonella enteritidis, serotype Horsham, identified rarely worldwide, was isolated from the stools of six American Indians with gastroenteritis. The patients were from separate tribes located in three geographically distinct villages. Although significant contact was demonstrated between ill individuals within two tr...

journal_title：Journal of clinical microbiology

authors： Castle SP,Nims LJ,Lapham SC

更新日期：1983-07-01 00:00:00

abstract：:The use of monoclonal antibody (MAb) p63-27, which is reactive with the major immediate-early human cytomegalovirus (HCMV) protein pp72, was explored for the rapid diagnosis of HCMV viruria. The rapid assay detected all but 1 of 19 specimens identified by standard virus isolation methods from 1,676 newborn urine speci...

journal_title：Journal of clinical microbiology

authors： Boppana SB,Smith RJ,Stagno S,Britt WJ

更新日期：1992-03-01 00:00:00

abstract：:Syrian hamsters were shown to be susceptible to infection by the Lyme disease spirochete Borrelia burgdorferi. Although these spirochetes did not cause any outward signs of illness in these animals, they did cause a generalized infection. Spirochetemia was present during the first 6 days of infection. At 14 days posti...

journal_title：Journal of clinical microbiology

authors： Johnson RC,Marek N,Kodner C

更新日期：1984-12-01 00:00:00

abstract：:Sixty-one Salmonella enterica serovar Typhimurium isolates of animal and human origin, matched by phage type, antimicrobial resistance pattern, and place of isolation, were analyzed by microbiological and molecular techniques, including pulsed-field gel electrophoresis (PFGE) and plasmid profiling. PFGE identified 10 ...

journal_title：Journal of clinical microbiology

authors： Cooke FJ,Wain J,Fookes M,Ivens A,Thomson N,Brown DJ,Threlfall EJ,Gunn G,Foster G,Dougan G

更新日期：2007-08-01 00:00:00

abstract：:An optimized reverse transcription (RT)-PCR protocol for the intravitam detection of rabies virus genomic RNA was tested with clinical samples obtained from 28 patients suspected of having rabies, 9 of whom were confirmed to have had rabies by postmortem examination. RT-PCR using saliva combined with an immunofluoresc...

journal_title：Journal of clinical microbiology

authors： Crepin P,Audry L,Rotivel Y,Gacoin A,Caroff C,Bourhy H

更新日期：1998-04-01 00:00:00

abstract：:Reverse transcription-PCR (RT-PCR) is a sensitive method for detection of RNA virus nucleic acid sequences in clinical respiratory specimens. Previous studies have focused on RT-PCR for a single virus, but this approach is limited by the inability to establish a specific etiology when the RT-PCR result is negative and...

journal_title：Journal of clinical microbiology

authors： Gilbert LL,Dakhama A,Bone BM,Thomas EE,Hegele RG

更新日期：1996-01-01 00:00:00

abstract：:During 1994, a cross-contamination problem leading to false-positive cultures of Mycobacterium tuberculosis was revealed in a mycobacteriology laboratory processing 30,000 to 35,000 samples per year. Molecular strain typing based on restriction fragment length polymorphism confirmed the contaminations. Out of 1,439 po...

journal_title：Journal of clinical microbiology

authors： Bauer J,Thomsen VO,Poulsen S,Andersen AB

更新日期：1997-04-01 00:00:00

abstract：:We compared examination of unstained and Gram-stained smears as methods of evaluating sputum quality. Of 100 slides examined, 96 (96%) were graded identically. Each discrepancy between methods was near the cutoff for specimen acceptability. We concluded that examination of unstained smears can provide an accurate, cos...

journal_title：Journal of clinical microbiology

authors： Wetterau LM,Zeimis RT,Hollick GE

更新日期：1986-07-01 00:00:00