Intravitam diagnosis of human rabies by PCR using saliva and cerebrospinal fluid.

abstract：:The accuracy of disk susceptibility tests with ampicillin and ampicillin-sulbactam was not improved when the amount of ampicillin was increased from 10 to 20 or 30 micrograms per disk. For testing members of the family Enterobacteriaceae, ampicillin disk tests correlated better with broth microdilution tests when the ...

journal_title：Journal of clinical microbiology

authors： Barry AL,Jones RN

更新日期：1988-04-01 00:00:00

abstract：:The susceptibilities of 54 clinical isolates of Neisseria meningitidis to penicillin, cefotaxime, ceftriaxone, cefepime, imipenem, ciprofloxacin, chloramphenicol, and rifampin were determined by the microdilution method in both cation-adjusted Mueller-Hinton broth (CAMHB) and Haemophilus test medium (HTM). Poor growth...

journal_title：Journal of clinical microbiology

authors： Pascual A,Joyanes P,Martinez-Martinez L,Suarez AI,Perea EJ

更新日期：1996-03-01 00:00:00

abstract：:A 128-member specimen pooling scheme for acute HIV infection (AHI) detection was evaluated using 21 AHI specimens (range, 1,520 to 500,000 copies/ml) previously identified by RNA testing of 16-member plasma pools. HIV-1 RNA was detectable in 128-member pools for all 21 specimens; however, one pool created from a speci...

journal_title：Journal of clinical microbiology

authors： Sullivan TJ,Patel P,Hutchinson A,Ethridge SF,Parker MM

更新日期：2011-10-01 00:00:00

abstract：:Human milk obtained from 21 American nursing mothers was studied for the presence of secretory immunoglobulin A antibody to rotavirus, the most common etiological agent of infantile gastroenteritis. Antibody was quantitated by adaptation of a recently described solid-phase radioimmunoassay technique that employs simia...

journal_title：Journal of clinical microbiology

authors： Cukor G,Blacklow NR,Capozza FE,Panjvani ZF,Bednarek F

更新日期：1979-01-01 00:00:00

abstract：:Phosphatase activity was found to be applicable as a criterion for the differentiation of Mycoplasma salivarium and Mycoplasma orale, predominant constituents of oral mycoplasmal flora. Therefore, a simple procedure for the phosphatase activity assay was established as a screening test for the differentiation of oral ...

journal_title：Journal of clinical microbiology

authors： Shibata K,Totsuka M,Watanabe T

更新日期：1986-05-01 00:00:00

abstract：:This report describes an unusual pathogen associated with post-traumatic meningitis. Haemophilus influenzae type D was isolated from both cerebrospinal fluid and blood of this patient with meningitis. ...

journal_title：Journal of clinical microbiology

authors： Viner JP,Massanari RM

更新日期：1979-02-01 00:00:00

abstract：:Variable-number tandem repeats (VNTRs) may evolve so rapidly that multiple profiles emerge during an outbreak. A total of 190 isolates from eight Salmonella enterica serovar Typhimurium outbreaks and 15 isolates from seven patients were analyzed by pulsed-field gel electrophoresis and VNTR typing. Small changes in loc...

journal_title：Journal of clinical microbiology

authors： Hopkins KL,Maguire C,Best E,Liebana E,Threlfall EJ

更新日期：2007-09-01 00:00:00

abstract：:Two multiplex PCR enzyme immunoassays (PCR-EIAs) were developed for Staphylococcus aureus exotoxin gene screening as an alternative to the conventional biological assays, which depend on detectable amounts of toxins produced. One set of oligonucleotide primers and probes was designed to search for enterotoxin A to E g...

journal_title：Journal of clinical microbiology

authors： Becker K,Roth R,Peters G

更新日期：1998-09-01 00:00:00

abstract：:We describe two rapid, simple, and reliable procedures for routine purification of hepatitis B virus (HBV) DNA from serum. HBV DNA could be purified from 24 serum samples in 1.5 to 2 h and was recovered in the initial reaction vessel. Both procedures have in common that HBV DNA is complexed with silica particles in th...

journal_title：Journal of clinical microbiology

authors： Boom R,Sol CJ,Heijtink R,Wertheim-van Dillen PM,van der Noordaa J

更新日期：1991-09-01 00:00:00

abstract：:We compared a set of commercial Salmonella somatic and flagellar serotyping antisera to in-house-prepared antisera from the Microbial Diseases Laboratory, California Department of Public Health, using 327 Salmonella enterica strains belonging to subgroups I, II, IIIa, IIIb, and IV. The sensitivities of Denka Seiken (T...

journal_title：Journal of clinical microbiology

authors： Schrader KN,Fernandez-Castro A,Cheung WK,Crandall CM,Abbott SL

更新日期：2008-02-01 00:00:00

abstract：:Ninety-nine isolates of clinical origin, tentatively identified as Scopulariopsis or Microascus, were morphologically and molecularly characterized by a combined analysis of the D1/D2 domains of the 28S rRNA gene and a fragment of the elongation factor 1-α gene (EF1-α) sequences. The most prevalent species was Scopula...

journal_title：Journal of clinical microbiology

authors： Sandoval-Denis M,Sutton DA,Fothergill AW,Cano-Lira J,Gené J,Decock CA,de Hoog GS,Guarro J

更新日期：2013-12-01 00:00:00

abstract：:The usefulness of randomly amplified polymorphic DNA method (RAPD) to identify each species of genus Erysipelothrix and for epidemiological analysis of this genus was studied. Eighty-one strains and 18 random primers were tested. Among the tested primers, the primers NK51 (GGTGGTGGTATC) and NK6 (CCCGCGCCCC) produced n...

journal_title：Journal of clinical microbiology

authors： Okatani AT,Hayashidani H,Takahashi T,Taniguchi T,Ogawa M,Kaneko K

更新日期：2000-12-01 00:00:00

abstract：:We compared the BD MAX enteric bacterial panel (EBP) to culture for the detection of Salmonella, Shigella, Campylobacter, and Shiga toxin-producing enterohemorrhagic Escherichia coli (EHEC) O157 in seeded stool samples. The EBP panel demonstrated superior sensitivity and reliably detected Salmonella, EHEC O157, Shigel...

journal_title：Journal of clinical microbiology

authors： Anderson NW,Buchan BW,Ledeboer NA

更新日期：2014-04-01 00:00:00

abstract：:Three susceptibility testing procedures were compared to determine fluconazole, itraconazole, and ketoconazole MICs against 47 Candida albicans strains isolated sequentially from the oral cavities of five AIDS patients undergoing azole therapy. They included the broth microdilution method (BM), performed according to ...

journal_title：Journal of clinical microbiology

authors： Tortorano AM,Viviani MA,Barchiesi F,Arzeni D,Rigoni AL,Cogliati M,Compagnucci P,Scalise G

更新日期：1998-06-01 00:00:00

abstract：:The recent emergence of carbapenemase-producing Enterobacteriaceae strains represents a major threat for hospitalized patients. We document the dissemination and control of carbapenemase-producing Klebsiella pneumoniae clones in a Greek hospital. During a 3-year study period (January 2009 to December 2011), carbapenem...

journal_title：Journal of clinical microbiology

authors： Poulou A,Voulgari E,Vrioni G,Xidopoulos G,Pliagkos A,Chatzipantazi V,Markou F,Tsakris A

更新日期：2012-08-01 00:00:00

abstract：:Participants in the College of American Pathologists' Mycobacteriology E proficiency testing survey in 1993 were asked to complete a questionnaire addressing mycobacterial test methods, test volume, and frequency of detection of drug-resistant Mycobacterium tuberculosis (MTB). A similar questionnaire had been distribu...

journal_title：Journal of clinical microbiology

authors： Woods GL,Witebsky FG

更新日期：1995-02-01 00:00:00

abstract：:A survey in 2000 to detect methicillin-resistant Staphylococcus aureus (MRSA) colonization in Vancouver downtown east side injection drug users (IDUs) revealed an MRSA nasal colonization incidence of 7.4%. This is a follow-up study to determine the current prevalence of MRSA colonization and to further characterize th...

journal_title：Journal of clinical microbiology

authors： Al-Rawahi GN,Schreader AG,Porter SD,Roscoe DL,Gustafson R,Bryce EA

更新日期：2008-02-01 00:00:00

abstract：:Ninety-one isolates of nutritionally variant streptococci (NVS) that were previously isolated from the human mouth were regarded as consisting of 7 Streptococcus defectivus isolates, 78 Streptococcus adjacens isolates, and 6 Gemella morbillorum isolates. However, recent references to the taxonomic reclassification of ...

journal_title：Journal of clinical microbiology

authors： Sato S,Kanamoto T,Inoue M

更新日期：1999-08-01 00:00:00

abstract：:The DNA hybridization assay for genes encoding for Escherichia coli enterotoxins was used to examine water specimens in Thailand. In a reconstruction experiment, the DNA hybridization assay was 10(4) times more sensitive than testing random E. coli in the Y-1 adrenal and suckling mouse assays in identifying enterotoxi...

journal_title：Journal of clinical microbiology

authors： Echeverria P,Seriwatana J,Chityothin O,Chaicumpa W,Tirapat C

更新日期：1982-12-01 00:00:00

abstract：:RotaTeq (RV5) is a widely used live attenuated pentavalent rotavirus (RV) vaccine. Although fecal shedding of RV vaccine strains persists for long time periods, it is unclear how each vaccine strain replicates in intestinal tissue and is excreted in stool. To examine this issue, we established RV5 genotype-specific re...

journal_title：Journal of clinical microbiology

authors： Higashimoto Y,Ihira M,Miyazaki Y,Kuboshiki A,Yoshinaga S,Hiramatsu H,Suzuki R,Miyata M,Miura H,Komoto S,Yukitake J,Taniguchi K,Kawamura Y,Yoshikawa T

更新日期：2018-05-25 00:00:00

abstract：:Gram stains remain the cornerstone of diagnostic testing in the microbiology laboratory for the guidance of empirical treatment prior to availability of culture results. Incorrectly interpreted Gram stains may adversely impact patient care, and yet there are no comprehensive studies that have evaluated the reliability...

journal_title：Journal of clinical microbiology

authors： Samuel LP,Balada-Llasat JM,Harrington A,Cavagnolo R

更新日期：2016-06-01 00:00:00

abstract：:Acinetobacter spp. are important nosocomial pathogens reported with increasing frequency in outbreaks of cross-infection during the past 2 decades. The majority of such outbreaks are caused by Acinetobacter baumannii. To investigate whether desiccation tolerance may be involved in the ability of certain strains of A. ...

journal_title：Journal of clinical microbiology

authors： Jawad A,Seifert H,Snelling AM,Heritage J,Hawkey PM

更新日期：1998-07-01 00:00:00

abstract：:An indirect immunofluorescence test with total anti-human immunoglobulin conjugate (IgG,A,M-IIF) can be used for joint detection of immunoglobulin A (IgA) and IgM antibodies, provided serum IgG is previously absorbed with anti-human IgG. To determine the validity of the IgG,A,M-IIF assay with absorbed sera, the result...

journal_title：Journal of clinical microbiology

authors： Arcavi M,Orfus G,Griemberg G

更新日期：1997-06-01 00:00:00

abstract：:Thirty-six Shiga toxin-producing Escherichia coli (STEC) O111:H- strains, 18 of which were isolated from patients with hemolytic-uremic syndrome (HUS) and 18 from patients suffering from diarrhea, were investigated for their enterohemolytic phenotypes and genotypes. Twenty-two strains were EHEC hemolysin (EHEC Hly) po...

journal_title：Journal of clinical microbiology

authors： Schmidt H,Karch H

更新日期：1996-10-01 00:00:00

abstract：:This is the first reported case of native valve endocarditis caused by Engyodontium album. This fungus, rarely seen as a human pathogen, is separated from Tritirachium species by its lack of pigmentation and from Beauveria species by the presence of conidiogenous cells in whorls. ...

journal_title：Journal of clinical microbiology

authors： Augustinsky J,Kammeyer P,Husain A,deHoog GS,Libertin CR

更新日期：1990-06-01 00:00:00

abstract：:We evaluated a new hepatitis C virus RNA assay based on one-stage PCR followed by liquid hybridization with an oligonucleotide probe and compared it with nested-set PCR. The one-stage and nested-set PCR assays had identical sensitivities in analytical experiments and showed 100% concordance when clinical specimens wer...

journal_title：Journal of clinical microbiology

authors： Gretch DR,Wilson JJ,Carithers RL Jr,dela Rosa C,Han JH,Corey L

更新日期：1993-02-01 00:00:00

abstract：:The use of high-risk human papillomavirus (HPV) testing for surveillance and clinical applications is increasing globally, and it is important that tests are evaluated to ensure they are fit for this purpose. In this study, the performance of a new HPV genotyping test, the Papilloplex high-risk HPV (HR-HPV) test, was ...

journal_title：Journal of clinical microbiology

authors： Bhatia R,Serrano I,Wennington H,Graham C,Cubie H,Boland E,Fu G,Cuschieri K

更新日期：2018-02-22 00:00:00

abstract：:To determine the excretion dynamics and genotypic characteristics of rotavirus A (RVA), a longitudinal observational study was performed in 10 pigs from 3 litters at a farrow-to-finish farm. A total of 400 fecal samples were directly collected from the rectums of individual pigs (aged 7 to 217 days) at 3- to 14-day in...

journal_title：Journal of clinical microbiology

authors： Miyazaki A,Kuga K,Suzuki T,Tsunemitsu H

更新日期：2012-06-01 00:00:00

abstract：:A total of 244 strains of Aerococcus, Enterococcus, and Streptococcus species were tested by the RapID STR system (Innovative Diagnostic Systems Inc., Atlanta, Ga.) for identification. Strains were identified without additional tests or with additional conventional tests suggested by the IDS compendium manual. Our dat...

journal_title：Journal of clinical microbiology

authors： You MS,Facklam RR

更新日期：1986-10-01 00:00:00

abstract：:We recently tested serum from a human immunodeficiency virus-infected patient for the presence of cryptococcal antigen using the Meridian latex agglutination (LA) test (Cryptococcal Antigen Latex Agglutination System). Two pronase-treated serum specimens from the patient had LA titers of 80 and 160, but the patient ha...

journal_title：Journal of clinical microbiology

authors： Whittier S,Hopfer RL,Gilligan P

更新日期：1994-09-01 00:00:00