Randomly amplified polymorphic DNA analysis of Erysipelothrix spp.

abstract：:A genomics-based PCR method was developed and used to test specimens from patients involved in a large outbreak of Mycoplasma pneumoniae in a closed religious community in New York State. New P1 adhesin gene primers were designed to bind to 9 of 10 target sequences in the repetitive-element sequences obtained from the...

journal_title：Journal of clinical microbiology

authors： Waring AL,Halse TA,Csiza CK,Carlyn CJ,Arruda Musser K,Limberger RJ

更新日期：2001-04-01 00:00:00

abstract：:Replication of Sendai virus, pneumonia virus of mice, and SV5 was investigated in tracheal organ cultures from 2- to 4-day-old and 2- and 4-week-old hamsters, and viral infectivity in tracheal explants was compared with that in tissue culture monolayers. Explants from 2- to 4-day-old hamsters produced higher titers of...

journal_title：Journal of clinical microbiology

authors： Schiff LJ

更新日期：1976-09-01 00:00:00

abstract：:In an evaluation of the API 20NE for the identification of Burkholderia spp., 792/800 (99%) Burkholderia pseudomallei and 17/19 (89%) B. cepacia isolates were correctly identified but 10 B. mallei and 98 B. thailandensis isolates were not correctly identified. A latex agglutination test was positive for 796/800 (99.5%...

journal_title：Journal of clinical microbiology

authors： Amornchai P,Chierakul W,Wuthiekanun V,Mahakhunkijcharoen Y,Phetsouvanh R,Currie BJ,Newton PN,van Vinh Chau N,Wongratanacheewin S,Day NP,Peacock SJ

更新日期：2007-11-01 00:00:00

abstract：:The genetic variation within a bacterial clone (known as Shigella sonnei but in effect a clone of Escherichia coli) was examined by studying 46 clinical isolates that were epidemiologically unassociated and isolated from patients in different countries over a period of 41 years (1950 to 1991). Restriction fragment len...

journal_title：Journal of clinical microbiology

authors： Karaolis DK,Lan R,Reeves PR

更新日期：1994-03-01 00:00:00

abstract：:At various times after orthopedic operations (more than a few weeks, with an average of 29.9 days), 11 patients had a sudden onset of high temperature (average 38.9 degrees C) and local cellulitis at different sites on the operated sides. The wounds had completely healed, without complicated infections, when the cellu...

journal_title：Journal of clinical microbiology

authors： Kitamura T,Kawamura Y,Ohkusu K,Masaki T,Iwashita H,Sawa T,Fujii S,Okamoto T,Akaike T

更新日期：2007-01-01 00:00:00

abstract：:An apparent outbreak of serious Candida albicans infections (n = 6) occurred in a surgical intensive care unit over a 4-week period. Four patients developed C. albicans bloodstream infections. An additional patient developed catheter-related C. albicans infection; the sixth patient developed an infection of cerebrospi...

journal_title：Journal of clinical microbiology

authors： Voss A,Pfaller MA,Hollis RJ,Rhine-Chalberg J,Doebbeling BN

更新日期：1995-03-01 00:00:00

abstract：:High-risk human papillomavirus (hrHPV) testing is now being introduced as a potential primary screening test for improved detection of cervical precancer and cancer. Current U.S. Food and Drug Administration-approved tests are batch tests that take several hours to complete. A rapid, non-batch test might permit point-...

journal_title：Journal of clinical microbiology

authors： Einstein MH,Smith KM,Davis TE,Schmeler KM,Ferris DG,Savage AH,Gray JE,Stoler MH,Wright TC Jr,Ferenczy A,Castle PE

更新日期：2014-06-01 00:00:00

abstract：:Multidrug-resistant tuberculosis is an increasing health problem worldwide, especially in developing countries. The PCR-UHG-Rif assay, which detects mutations within the rpoB gene associated with rifampin resistance, was evaluated for its ability and reliability to detect and identify drug-resistant Mycobacterium tube...

journal_title：Journal of clinical microbiology

authors： Mayta H,Gilman RH,Arenas F,Valencia T,Caviedes L,Montenegro SH,Ticona E,Ortiz J,Chumpitaz R,Evans CA,Williams DL,Tuberculosis Working Group in Perú.

更新日期：2003-12-01 00:00:00

abstract：:Specimens from skin lesions were examined simultaneously for herpes simplex virus (HSV) and varicella-zoster virus (VZV) by direct specimen testing and shell vial culture in single-test systems. For direct testing, cells in a single specimen well were stained with a combination direct-indirect immunofluorescence stain...

journal_title：Journal of clinical microbiology

authors： Brumback BG,Farthing PG,Castellino SN

更新日期：1993-12-01 00:00:00

abstract：:In this study, we compared a recently developed PCR-restriction fragment length polymorphism (PCR-RFLP) assay with pulsed-field gel electrophoresis (PFGE) using three different Shiga toxin-producing Escherichia coli (STEC) strains to understand whether repeated subculture in vitro and prolonged storage at room tempera...

journal_title：Journal of clinical microbiology

authors： Shima K,Wu Y,Sugimoto N,Asakura M,Nishimura K,Yamasaki S

更新日期：2006-11-01 00:00:00

abstract：:Samples from patients at high risk for invasive aspergillosis (IA) were prospectively collected and analyzed for the presence of molecular markers of fungal infection. Serum specimens were screened for galactomannan and Aspergillus DNA, and whole-blood specimens were screened only for Aspergillus DNA. Fungal infection...

journal_title：Journal of clinical microbiology

authors： Springer J,Morton CO,Perry M,Heinz WJ,Paholcsek M,Alzheimer M,Rogers TR,Barnes RA,Einsele H,Loeffler J,White PL

更新日期：2013-05-01 00:00:00

abstract：:The effects of Tween 80 supplementation of liquid culture medium on the formation of the superficial L1 layer of the Mycobacterium avium-Mycobacterium intracellulare complex (MAC) were examined by serological and scanning electron microscopic experiments. Specific antiserum to the glycopeptidolipids on the L1 layer of...

journal_title：Journal of clinical microbiology

authors： Masaki S,Sugimori G,Okamoto A,Imose J,Hayashi Y

更新日期：1991-07-01 00:00:00

abstract：:The ability of Staphylococcus aureus to clot plasma through conformational activation of prothrombin by staphylocoagulase is used to distinguish S. aureus from coagulase-negative staphylococci. We show that while the direct thrombin inhibitor dabigatran inhibits staphylocoagulase activity, the clinical use of dabigatr...

journal_title：Journal of clinical microbiology

authors： Vanassche T,Verhaegen J,Peetermans WE,Hoylaerts MF,Verhamme P

更新日期：2010-11-01 00:00:00

abstract：:We report a case of catheter-related bloodstream infection by Tsukamurella inchonensis, identified using 16S rRNA gene sequencing, in a patient with myelofibrosis who underwent a bone marrow transplant. Tsukamurella species infections are rare. To our knowledge, this is the first case of T. inchonensis bloodstream inf...

journal_title：Journal of clinical microbiology

authors： Takebe I,Sawabe E,Ohkusu K,Tojo N,Tohda S

更新日期：2014-06-01 00:00:00

abstract：:Streptococcus mutans strains were isolated from cohorts of Brazilian nursery school children and genotyped by arbitrarily primed PCR and restriction fragment length polymorphism analysis. Of 24 children with two to five S. mutans isolates, 29% carried two or more genotypes. The presence of matching genotypes of S. mut...

journal_title：Journal of clinical microbiology

authors： Mattos-Graner RO,Li Y,Caufield PW,Duncan M,Smith DJ

更新日期：2001-06-01 00:00:00

abstract：:Haemophilus ducreyi ATCC 33922, a virulent, well-characterized strain, was used to construct a genomic library in a bacteriophage expression vector. Three DNA fragments were selected for use as probes on the basis of their ability to encode H. ducreyi-specific proteins, as demonstrated by reactivity with rabbit polycl...

journal_title：Journal of clinical microbiology

authors： Parsons LM,Shayegani M,Waring AL,Bopp LH

更新日期：1989-07-01 00:00:00

abstract：:We studied the evolution of Vibrio cholerae O1 during the 1991 to 2000 cholera epidemic in Mexico by biochemical, serological, and molecular characterization of strains collected during this period. Strains were divided into toxigenic and nontoxigenic groups according to the presence or absence of genes encoding chole...

journal_title：Journal of clinical microbiology

authors： Lizárraga-Partida ML,Quilici ML

更新日期：2009-05-01 00:00:00

abstract：:The studies reported here were undertaken to assess the ability of the outer membrane proteins (OMPs) of Salmonella typhi to induce a humoral immune response in humans with typhoid fever. OMPs were isolated with the nonionic detergent Triton X-100 and were found to be contaminated with approximately 4% lipopolysacchar...

journal_title：Journal of clinical microbiology

authors： Ortiz V,Isibasi A,García-Ortigoza E,Kumate J

更新日期：1989-07-01 00:00:00

abstract：:Because of its low yield in unselected specimens, stool culture is often cost ineffective. We tested 55 fecal samples from Fairfax Hospital (46 patients with diarrhea and 9 from controls without diarrhea) for lactoferrin by latex agglutination (LFLA) with the Leukotest (Techlab, Blacksburg, Va.) as a marker for inflam...

journal_title：Journal of clinical microbiology

authors： Choi SW,Park CH,Silva TM,Zaenker EI,Guerrant RL

更新日期：1996-04-01 00:00:00

abstract：:This study evaluated the performance of Directigen FluA combined with a 3-day flu screening culture for the detection of influenza virus. This abbreviated protocol was a useful and effective tool and resulted in a substantial reduction in time, effort, and money spent, while not compromising sensitivity of influenza v...

journal_title：Journal of clinical microbiology

authors： Newton DW,Mellen CF,Baxter BD,Atmar RL,Menegus MA

更新日期：2002-11-01 00:00:00

abstract：:A growth medium with a specific oxidation-reduction potential containing peptone, dextrose, sodium succinate, sodium lactate, gelatin, sodium bicarbonate and blue tetrazolium, an indicator dye, in a tris(hydroxymethyl)aminomethane buffer was used to detect the presence of microorganisms in blood. The procedure involve...

journal_title：Journal of clinical microbiology

authors： Shih CN,Balish E

更新日期：1977-09-01 00:00:00

abstract：:We propose a novel application of secondary electrospray ionization-mass spectrometry (SESI-MS) as a real-time clinical diagnostic tool for bacterial infection. It is known that volatile organic compounds (VOCs), produced in different combinations and quantities by bacteria as metabolites, generate characteristic odor...

journal_title：Journal of clinical microbiology

authors： Zhu J,Bean HD,Kuo YM,Hill JE

更新日期：2010-12-01 00:00:00

abstract：:The reliability of the 10-micrograms clindamycin disk was evaluated for susceptibility testing of anaerobes by the aerobic thioglycolate broth disk method. A good correlation between the aerobic thioglycolate broth disk method and the reference agar dilution procedure of the National Committee for Clinical Laboratory ...

journal_title：Journal of clinical microbiology

authors： Toohey KL,Kurzynski TA,Schell RF,Birk RJ,Zabransky RJ

更新日期：1986-03-01 00:00:00

abstract：:Recent Lassa virus strains from Nigeria were completely or partially sequenced. Phylogenetic analysis revealed the predominance of lineage II and III strains, the existence of a previously undescribed (sub)lineage in Nigeria, and the directional spread of virus in the southern part of the country. The Bayesian analysi...

journal_title：Journal of clinical microbiology

authors： Ehichioya DU,Hass M,Becker-Ziaja B,Ehimuan J,Asogun DA,Fichet-Calvet E,Kleinsteuber K,Lelke M,ter Meulen J,Akpede GO,Omilabu SA,Günther S,Olschläger S

更新日期：2011-03-01 00:00:00

abstract：:We compared trends of annual resistance rates calculated from results for all isolates and for the first isolate of Staphylococcus aureus, Klebsiella pneumoniae, and Acinetobacter baumannii per patient over a 3-year period from 2001 through 2003. Antimicrobial susceptibility results of inpatients were extracted from a...

journal_title：Journal of clinical microbiology

authors： Lee SO,Cho YK,Kim SY,Lee ES,Park SY,Seo YH

更新日期：2004-10-01 00:00:00

abstract：:Two in a group of five naturally seropositive donkey stallions were found to shed equine arteritis virus (EAV) in their semen as demonstrated by virus isolation. Direct intramuscular inoculation of sonicated semen from one virus-shedding stallion (S3) caused clinical disease in two donkeys from which virus was recover...

journal_title：Journal of clinical microbiology

authors： Paweska JT,Volkmann DH,Barnard BJ,Chirnside ED

更新日期：1995-12-01 00:00:00

abstract：:Fecal specimens from patients with acute diarrhea were collected from 10 prefectures in Japan over a 6-month period (November 1992 to April 1993), and the specimens that were negative for human group A rotaviruses were screened for the presence of human group C rotaviruses (CHRVs) by the reverse passive hemagglutinati...

journal_title：Journal of clinical microbiology

authors： Kuzuya M,Fujii R,Hamano M,Yamada M,Shinozaki K,Sasagawa A,Hasegawa S,Kawamoto H,Matsumoto K,Kawamoto A,Itagaki A,Funatsumaru S,Urasawa S

更新日期：1998-01-01 00:00:00

abstract：:The usefulness of pneumococcal urinary antigen tests (UATs) in severe pneumococcal infection relies heavily on the performance in bacteremic patients. Fluorescence technology and automatic reading of test results may improve UAT performance. We evaluated the automatically read Sofia S. pneumoniae FIA for diagnosing pn...

journal_title：Journal of clinical microbiology

authors： Olofsson E,Özenci V,Athlin S

更新日期：2019-07-26 00:00:00

abstract：:The DNA fingerprint profiles and serotypes of 63 avian Pasteurella multocida field isolates, 13 attenuated vaccine isolates (propagated from vaccines manufactured by five companies), and 16 somatic reference strains were compared. DNA fingerprinting established the relationship of isolates that could not be distinguis...

journal_title：Journal of clinical microbiology

authors： Wilson MA,Morgan MJ,Barger GE

更新日期：1993-02-01 00:00:00

abstract：:A real-time PCR assay was developed targeting the bla(KPC) responsible for Klebsiella pneumoniae carbapenemase (KPC)-mediated carbapenem resistance and was validated for testing colonies or enrichment broth cultures. The assay accurately detects KPC-containing strains with high analytical specificity and sensitivity. ...

journal_title：Journal of clinical microbiology

authors： Mangold KA,Santiano K,Broekman R,Krafft CA,Voss B,Wang V,Hacek DM,Usacheva EA,Thomson RB Jr,Kaul KL,Peterson LR

更新日期：2011-09-01 00:00:00