Genotypic diversity of mutans streptococci in Brazilian nursery children suggests horizontal transmission.

abstract：:A study to compare the polymerase chain reaction (PCR) test with the cell culture method in diagnosing urogenital Chlamydia trachomatis infections was performed. From 497 patients (212 women, 285 men) attending an outpatient clinic for sexually transmitted diseases, a total of 814 samples (female patients, cervix and ...

journal_title：Journal of clinical microbiology

authors： Vogels WH,van Voorst Vader PC,Schröder FP

更新日期：1993-05-01 00:00:00

abstract：:Neisseria meningitidis is the causative agent of meningococcal sepsis and meningitis. Neisseria polysaccharea is a nonpathogenic species. N. polysaccharea is able to use sucrose to produce amylopectin, a starch-like polysaccharide, which distinguishes it biochemically from the pathogenic species N. meningitidis. The d...

journal_title：Journal of clinical microbiology

authors： Zhu P,Tsang RS,Tsai CM

更新日期：2003-01-01 00:00:00

abstract：:CHROMagar Yersinia (CAY) is a new chromogenic medium for the presumptive detection of virulent Yersinia enterocolitica in stools. Based on a comparative analysis of 1,494 consecutive stools from hospitalized patients, CAY was found to be just as sensitive as the reference medium (cefsulodin-irgasan-novobiocin agar) bu...

journal_title：Journal of clinical microbiology

authors： Renaud N,Lecci L,Courcol RJ,Simonet M,Gaillot O

更新日期：2013-04-01 00:00:00

abstract：:The recovery of Nocardia species from mixed cultures is facilitated by use of a selective medium. We show that buffered charcoal-yeast extract medium with polymyxin, anisomycin, and vancomycin can be used for the selective isolation of nocardiae from contaminated specimens. ...

journal_title：Journal of clinical microbiology

authors： Garrett MA,Holmes HT,Nolte FS

更新日期：1992-07-01 00:00:00

abstract：:New variants of the influenza A(H1N1)pdm09 and A(H3N2) viruses were detected in Taiwan between 2012 and 2013. Some of these variants were not detected in clinical specimens using a common real-time reverse transcription-PCR (RT-PCR) assay that targeted the conserved regions of the viral matrix (M) genes. An analysis o...

journal_title：Journal of clinical microbiology

authors： Yang JR,Kuo CY,Huang HY,Wu FT,Huang YL,Cheng CY,Su YT,Chang FY,Wu HS,Liu MT

更新日期：2014-01-01 00:00:00

abstract：:Direct enzyme-linked immunosorbent assay methods offer several advantages in assessing past or recent exposure to cytomegalovirus (CMV) infection, but there persist many pitfalls in the use of these methods for determining specific immunoglobulin M (IgM). The efficiency of absorption of sera by IgG-coated latex beads,...

journal_title：Journal of clinical microbiology

authors： Joassin L,Reginster M

更新日期：1986-03-01 00:00:00

abstract：:The detection of amplified products resulting from polymerase chain reactions (PCRs) remains a complicated process. To simplify the detection procedures, we developed a colorimetric microtiter plate hybridization assay for the specific detection of 5'-biotinylated PCR fragments of Mycobacterium leprae DNA. For this as...

journal_title：Journal of clinical microbiology

authors： van der Vliet GM,Hermans CJ,Klatser PR

更新日期：1993-03-01 00:00:00

abstract：:A cell-free growth medium for the opportunistic pathogenic ameba Balamuthia mandrillaris is presented. This represents an advance over the use of monkey kidney cells for growth of the amebas and can be helpful in isolation of these amebas from brain tissue from cases in which amebic meningoencephalitis is a diagnostic...

journal_title：Journal of clinical microbiology

authors： Schuster FL,Visvesvara GS

更新日期：1996-02-01 00:00:00

abstract：:Salmonella enterica serovar Heidelberg is the second most frequently occurring serovar in Quebec and the third-most prevalent in Canada. Given that conventional pulsed-field gel electrophoresis (PFGE) subtyping for common Salmonella serovars, such as S. Heidelberg, yields identical subtypes for the majority of isolate...

journal_title：Journal of clinical microbiology

authors： Bekal S,Berry C,Reimer AR,Van Domselaar G,Beaudry G,Fournier E,Doualla-Bell F,Levac E,Gaulin C,Ramsay D,Huot C,Walker M,Sieffert C,Tremblay C

更新日期：2016-02-01 00:00:00

abstract：:A newly developed enzyme immunoassay (EIA) was used to detect the presence of pneumococcal teichoic and lipoteichoic acids in cerebrospinal fluid (CSF) from patients with Streptococcus pneumoniae meningitis who were being treated with antibiotics. All initial CSF samples, which on culture grew S. pneumoniae, were posi...

journal_title：Journal of clinical microbiology

authors： Stuertz K,Merx I,Eiffert H,Schmutzhard E,Mäder M,Nau R

更新日期：1998-08-01 00:00:00

abstract：:Sixty rotavirus-positive stool specimens from children with diarrhea were classified into G and P genotypes. G typing was done by PCR and then by hybridization with G type-specific (G1 to G4) oligonucleotide probes, whereas nested PCR was performed for P typing. Thirty-nine samples could be classified into both G and ...

journal_title：Journal of clinical microbiology

authors： Husain M,Seth P,Dar L,Broor S

更新日期：1996-06-01 00:00:00

abstract：:A Legionella-like organism (strain 798-PA-H, ATCC 35999) isolated from a lung aspirate was shown by DNA hybridization to belong to the species Legionella hackeliae. Growth, gas-liquid chromatographic, and physiologic characteristics were consistent with those of the type strain of L. hackeliae, Lansing 2 (ATCC 35250)....

journal_title：Journal of clinical microbiology

authors： Wilkinson HW,Thacker WL,Steigerwalt AG,Brenner DJ,Ampel NM,Wing EJ

更新日期：1985-10-01 00:00:00

abstract：:The performance of a commercially available, rapid membrane enzyme immunoassay for influenza A and B virus detection was compared to that of viral culture in 4,092 respiratory specimens collected from patients presenting with respiratory symptoms during the 2002-2003 influenza season. The test's overall sensitivity wa...

journal_title：Journal of clinical microbiology

authors： Cazacu AC,Chung SE,Greer J,Demmler GJ

更新日期：2004-08-01 00:00:00

abstract：:A LightCycler-based PCR protocol was developed which targets the ospA gene for the identification and quantification of the different Borrelia burgdorferi sensu lato species in culture and in ticks, based on the use of a fluorescently labeled probe (HybProbe) and an internally labeled primer. The detection limit of th...

journal_title：Journal of clinical microbiology

authors： Rauter C,Oehme R,Diterich I,Engele M,Hartung T

更新日期：2002-01-01 00:00:00

abstract：:We report the first two cases of invasive human mycoses caused by the phaeoid ascomycete, Chaetomium perlucidum, and review the English literature regarding invasive Chaetomium infections. Fatal disseminated disease involving the brain, heart, lungs, and spleen is described in an acute myelogenous leukemia patient. A ...

journal_title：Journal of clinical microbiology

authors： Barron MA,Sutton DA,Veve R,Guarro J,Rinaldi M,Thompson E,Cagnoni PJ,Moultney K,Madinger NE

更新日期：2003-11-01 00:00:00

abstract：:Three hundred ninety single sera from adults in Japan, Indonesia, Singapore, and Papua New Guinea were tested for antibodies to human calicivirus (HCV) by a radioimmunoassay blocking test. A high prevalence of antibodies was observed in samples collected in Japan and Southeast Asia. Of 240 serum specimens collected fr...

journal_title：Journal of clinical microbiology

authors： Nakata S,Chiba S,Terashima H,Nakao T

更新日期：1985-10-01 00:00:00

abstract：:A group of 76 consecutive human immunodeficiency virus (HIV)-positive patients with fever of unknown origin (n = 52) or fever associated with pulmonary diseases was evaluated in order to assess the usefulness of PCR with peripheral blood in the diagnosis and follow-up of visceral leishmaniasis. We identified 10 cases ...

journal_title：Journal of clinical microbiology

authors： Pizzuto M,Piazza M,Senese D,Scalamogna C,Calattini S,Corsico L,Persico T,Adriani B,Magni C,Guaraldi G,Gaiera G,Ludovisi A,Gramiccia M,Galli M,Moroni M,Corbellino M,Antinori S

更新日期：2001-01-01 00:00:00

abstract：:Panels of hepatitis B virus surface antigen-positive sera from drug abusers were used to evaluate 14 commercial enzyme immunoassays from six companies for detecting hepatitis delta virus (HDV) markers. For detecting hepatitis delta virus antigen (HDAg), the Wellcome, Pasteur and Noctech assays had 100% sensitivity for...

journal_title：Journal of clinical microbiology

authors： Shattock AG,Morris MC

更新日期：1991-09-01 00:00:00

abstract：:By using the computer-assisted Dendron system to analyze the patterns of Southern blots probed with the repetitive sequence Ca3, we have compared oral isolates of Candida spp. from a group of 11 nonhospitalized patients with AIDS suffering from recurrent episodes of oral thrush in Leicester, England, with oral isolate...

journal_title：Journal of clinical microbiology

authors： Schmid J,Odds FC,Wiselka MJ,Nicholson KG,Soll DR

更新日期：1992-04-01 00:00:00

abstract：:Two rapid (1-h) assays for the detection of Staphylococcus aureus staphylocoagulase were developed by using the fluorogenic thrombin substrates N-t-boc-Val-Pro-Arg-7-amido-4-methylcoumarin (VPA) and N-t-boc-beta-benzyl-Asp-Pro-Arg-7-amido-4-methylocoumarin (BB). The assays were compared to the tube coagulase test and ...

journal_title：Journal of clinical microbiology

authors： Holliday MG,Ford M,Perry JD,Gould FK

更新日期：1999-04-01 00:00:00

abstract：:CENTA is a newly synthesized, beta-lactamase-labile, chromogenic cephalosporin reagent which changes color from light yellow (lambda maximum ca. 340 nm) to chrome yellow (lambda maximum ca. 405 nm) concomitant with hydrolysis of the beta-lactam ring. This compound offers promise as a diagnostic reagent comparable to o...

journal_title：Journal of clinical microbiology

authors： Jones RN,Wilson HW,Novick WJ Jr,Barry AL,Thornsberry C

更新日期：1982-05-01 00:00:00

abstract：:Two commercial assays that detect Mycobacterium tuberculosis complex (MTB) in clinical specimens by rRNA target amplification (AMTDII) and ligase chain reaction (LCx) were evaluated. The tests were applied to 457 respiratory (n = 273) and extrapulmonary (n = 184) specimens collected from 357 patients. The results were...

journal_title：Journal of clinical microbiology

authors： Piersimoni C,Callegaro A,Scarparo C,Penati V,Nista D,Bornigia S,Lacchini C,Scagnelli M,Santini G,De Sio G

更新日期：1998-12-01 00:00:00

abstract：:A simple method of storage and retrieval of serum samples for adenovirus serology is described. Complement-fixing antibodies remained stable for at least 5 years when stored at -10 degrees C or below. ...

journal_title：Journal of clinical microbiology

authors： Edwards EA

更新日期：1977-02-01 00:00:00

abstract：:We have previously demonstrated homology between a 181-bp fragment of IS6110 and DNA from mycobacteria other than tuberculosis-causing mycobacteria (MOTT). Genomic DNA from 14 strains of MOTT was digested with PvuII and was hybridized with a probe derived from the 181-bp fragment and the INS1/INS2 international standa...

journal_title：Journal of clinical microbiology

authors： McHugh TD,Newport LE,Gillespie SH

更新日期：1997-07-01 00:00:00

abstract：:Recent evidence supports a causal relationship between Bartonella (Rochalimaea) henselae, cat-scratch disease (CSD), and bacillary angiomatosis. Cats appear to be the primary reservoir. Blood from 19 cats owned by 14 patients diagnosed with CSD was cultured. Blood samples from cats owned by veterinary students (n = 25...

journal_title：Journal of clinical microbiology

authors： Kordick DL,Wilson KH,Sexton DJ,Hadfield TL,Berkhoff HA,Breitschwerdt EB

更新日期：1995-12-01 00:00:00

abstract：:A nonpigmented rapidly growing mycobacterium was isolated from wound liquid outflow, bone tissue biopsy, and excised skin tissue from a 31-year-old woman who suffered an accidental open right tibia fracture and prolonged stay in a river. The three isolates grew in 3 days at 24 to 37 degrees C. 16S rRNA sequence analys...

journal_title：Journal of clinical microbiology

authors： Adékambi T,Stein A,Carvajal J,Raoult D,Drancourt M

更新日期：2006-04-01 00:00:00

abstract：:The prevalence of coinfection of Campylobacter species in dogs was determined using four isolation methods. In 26% of the positive-testing stools, multiple Campylobacter species were identified. The use of multiple isolation methods as well as the time lapse between sampling and processing are important for detection ...

journal_title：Journal of clinical microbiology

authors： Koene MG,Houwers DJ,Dijkstra JR,Duim B,Wagenaar JA

更新日期：2004-02-01 00:00:00

abstract：:Sixteen metabolically deficient dwarf-colony mutants of Escherichia coli were isolated from urine culture and represented about 2% of all E. coli isolated during the same period. In 14 cases, mutants were isolated from debilitated patients: elderly persons or patients in the terminal stages of a chronic disease. In 15...

journal_title：Journal of clinical microbiology

authors： Borderon E,Horodniceanu T

更新日期：1978-12-01 00:00:00

abstract：:The prevalence of tick-borne infections has been steadily increasing in both number and geographic distribution in the United States and abroad. This increase, in conjunction with the continued recognition of novel pathogens transmitted by ticks, has made accurate diagnosis of these infections challenging. Mainstay se...

journal_title：Journal of clinical microbiology

authors： Buchan BW,Jobe DA,Mashock M,Gerstbrein D,Faron ML,Ledeboer NA,Callister SM

更新日期：2019-10-23 00:00:00

abstract：:The serotype, RNA electropherotype, and cross-protection properties of rotaviruses isolated from canine, simian, porcine, and human species were compared. The bovine strain B:USA:78:1A and the canine strain C:USA:81:2 were adapted to cell culture and cloned in this study. The other viruses, i.e., simian strain S:USA:7...

journal_title：Journal of clinical microbiology

authors： Gaul SK,Simpson TF,Woode GN,Fulton RW

更新日期：1982-09-01 00:00:00