Abstract:
:We have previously demonstrated homology between a 181-bp fragment of IS6110 and DNA from mycobacteria other than tuberculosis-causing mycobacteria (MOTT). Genomic DNA from 14 strains of MOTT was digested with PvuII and was hybridized with a probe derived from the 181-bp fragment and the INS1/INS2 international standard probe at high stringency. Multiple banding patterns were obtained from isolates of M. avium-M. intracellulare, M. fortuitum, M. kansasii, and M. malmoense. Differences in the banding patterns between and within species were obtained. This suggests that mycobacteria possess a family of IS3-like elements. The species of isolates suspected of being M. tuberculosis must be carefully determined before IS6110 restriction fragment length polymorphism analysis, and caution must be used in designing and evaluating diagnostic PCR tests based on this element.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
McHugh TD,Newport LE,Gillespie SHdoi
10.1128/JCM.35.7.1769-1771.1997subject
Has Abstractpub_date
1997-07-01 00:00:00pages
1769-71issue
7eissn
0095-1137issn
1098-660Xjournal_volume
35pub_type
杂志文章abstract::Plasmodium nucleic acids have been detected in serum and plasma, but there is little published data describing the diagnostic performance of malaria nucleic acid amplification tests (NAATs) using these specimen types. Previously, our group described a multiplex NAAT for the detection of dengue virus, Leptospira, and P...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01876-15
更新日期:2015-11-01 00:00:00
abstract::Ten characteristic strains of spirochetes (HT2, HT7, HT10, HT15, HT17, HT19, HT20, HT22, HT32, and HT59) isolated from Ixodes persulcatus adult ticks in Hokkaido, Japan, were selected and analyzed by polyacrylamide gel electrophoresis, with monoclonal and polyclonal antibodies, and by pulsed-field gel electrophoresis....
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.31.5.1388-1391.1993
更新日期:1993-05-01 00:00:00
abstract::Standard broth microdilution (with and without bovine serum albumin [BSA] supplementation), tube dilution, and agar dilution susceptibility tests were compared for determining ramoplanin MICs. With a data base of 246 clinical isolates of gram-positive bacteria from 33 U.S. sites, it was shown that (i) agar and tube di...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.32.5.1364-1365.1994
更新日期:1994-05-01 00:00:00
abstract::The stability of cervical specimens in SurePath preservative fluid for human papillomavirus (HPV) testing with Roche cobas 4800 was determined using a panel of 308 pooled specimens from a colposcopy referral population. The SurePath specimens appeared to be stable for up to 10 weeks at ambient temperature for HPV test...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01391-13
更新日期:2013-10-01 00:00:00
abstract::We compared the Difco ESP 384 blood culture system with the pediatric Septi-Chek system for the detection of bloodstream infections in pediatric patients. A total of 10,762 blood culture sets included an ESP aerobic bottle and a Septi-Chek bottle. From these cultures, a total of 278 organisms classified as probable pa...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.33.5.1084-1088.1995
更新日期:1995-05-01 00:00:00
abstract::Early diagnosis of Neisseria gonorrhoeae infections is important with regard to patients' health and infectivity. We report the development of a specific and sensitive TaqMan assay for the detection of N. gonorrhoeae in clinical samples. The target sequence is a 76-bp fragment of the 5' untranslated region of the opa ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.43.11.5653-5659.2005
更新日期:2005-11-01 00:00:00
abstract::A rapid (2.5 h) direct enzyme immunoassay (EIA) for Clostridium difficile toxin A was developed for clinical use. Specimen centrifugation and filtration were not required. The EIA detected toxin A levels in patient stool as low as 20 pg (2 ng/ml of stool). The test was 5,000 times more sensitive for toxin A than it wa...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.29.12.2724-2730.1991
更新日期:1991-12-01 00:00:00
abstract::Continuous cell lines were tested for their potential use in distinguishing pathogenic from nonpathogenic Listeria species. Listeria monocytogenes and Listeria ivanovii strains were lethal for mice, and culture filtrates were cytotoxic for cultured cells and hemolytic for sheep erythrocytes, while Listeria innocua, Li...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.25.8.1463-1466.1987
更新日期:1987-08-01 00:00:00
abstract::Helicobacter cetorum sp. nov. has been cultured from the stomach of Atlantic white-sided dolphins (Lagenorhynchus acutus) and the feces of Pacific white-sided (L. obliquidens) and Atlantic bottlenose (Tursiops truncatus) dolphins and a beluga whale (Delphinapterus leucas). H. cetorum has high homology to Helicobacter ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/jcm.41.7.2842-2848.2003
更新日期:2003-07-01 00:00:00
abstract::A new molecular assay, based on a rapid DNA extraction protocol, PCR, and hybridization to a specific probe in a nonradioactive microwell plate format was used to detect Mycoplasma pneumoniae in bronchoalveolar fluid specimens. The sensitivity of the assay was determined to be 10 to 100 organisms with M. pneumoniae re...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.35.6.1592-1594.1997
更新日期:1997-06-01 00:00:00
abstract::The semen plasma virus load is measured to ensure the safety of sperm processing during medically assisted procreation (MAP) for couples with a human immunodeficiency virus type 1 (HIV-1)-infected man. A practical, automated protocol using the COBAS Ampliprep CAP/CTM kit in the COBAS TaqMan96 system was developed to m...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.02131-08
更新日期:2009-09-01 00:00:00
abstract::We developed an accurate nonradioactive colony hybridization assay (NCHA) using a digoxigenin-labeled polynucleotide probe and an antidigoxigenin alkaline phosphatase conjugate for the identification of enterotoxigenic Escherichia coli (ETEC) harboring genes for colonization factor antigen I (CFA/I), coli surface anti...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.30.7.1823-1828.1992
更新日期:1992-07-01 00:00:00
abstract::A survey for carriers of Streptococcus pneumoniae among 573 children was undertaken. Throat and nasopharyngeal cultures from 303 children disclosed pneumococci in 89 (29.4%) nasopharyngeal swabs but in only 8 (2.6%) throat specimens. Carriers of pneumococci were detected with frequencies of 84.4 and 79.9% on aerobical...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.16.1.1-3.1982
更新日期:1982-07-01 00:00:00
abstract::Shotgun proteomics using liquid chromatography-tandem mass spectrometry (LC-MS/MS) was applied to detect β-lactamases in clinical Acinetobacter baumannii isolates. The correlation of the detection of β-lactamase proteins (rather than PCR detection of the corresponding genes) with the resistance phenotypes demonstrated...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00620-15
更新日期:2015-06-01 00:00:00
abstract::Mycobacteria include a large number of pathogens. Identification to species level is important for diagnoses and treatments. Here, we report the development of a Web-accessible database of the hsp65 locus sequences (http://msis.mycobacteria.info) from 149 out of 150 Mycobacterium species/subspecies. This database can ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.02602-10
更新日期:2011-06-01 00:00:00
abstract::Potential causes of congenital infection include Toxoplasma gondii and viruses such as cytomegalovirus (CMV), enterovirus, hepatitis C virus, herpes simplex virus types 1 and 2 (HSV-1 and -2), human herpesvirus types 6, 7, and 8, lymphocytic choriomeningitis virus, parvovirus, rubella virus, and varicella-zoster virus...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.43.10.5102-5110.2005
更新日期:2005-10-01 00:00:00
abstract::A collection of 147 isolates of Burkholderia pseudomallei, B. mallei, and B. thailandensis was characterized by multilocus sequence typing (MLST). The 128 isolates of B. pseudomallei, the causative agent of melioidosis, were obtained from diverse geographic locations, from humans and animals with disease, and from the...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/jcm.41.5.2068-2079.2003
更新日期:2003-05-01 00:00:00
abstract::The genotypes of 63 isolates of Aspergillus fumigatus obtained from three hospitals in different geographical areas and of eight culture collection strains were determined by multilocus enzyme electrophoresis. Twelve of the 17 enzymatic loci studied were polymorphic, giving rise to 48 different electrophoretic types. ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章,多中心研究
doi:10.1128/JCM.34.10.2559-2568.1996
更新日期:1996-10-01 00:00:00
abstract::Little information is available about the genetic variability of Leishmania populations and the possible correlations with ecoepidemiological features of leishmaniases. The present study was carried out in French Guiana, a country where cutaneous leishmaniases (CL) are endemic over the whole territory. The genetic pol...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.44.2.468-473.2006
更新日期:2006-02-01 00:00:00
abstract::It is not always possible to culture feces immediately, and appropriate methods for transport of human specimens, unlike those from animals, have not been fully evaluated. Therefore, we took serial subcultures in two phases from six transport media inoculated with human diarrheal stools known to be positive for Campyl...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.18.4.803-807.1983
更新日期:1983-10-01 00:00:00
abstract::The Elecsys Anti-SARS-CoV-2 immunoassay (Roche Diagnostics) was developed to provide accurate, reliable detection of antibodies to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We evaluated sensitivity, specificity, cross-reactivity, and agreement with a vesicular stomatitis virus-based pseudoneutraliz...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01694-20
更新日期:2020-09-22 00:00:00
abstract::A rapid dimethyl sulfoxide modification of an acid-fast technique was applied to direct fecal smears to monitor cryptosporidiosis in nonhuman primates. Brilliantly stained pink oocysts against a pale green background demonstrated well-preserved internal morphology and facilitated rapid, simple, noninvasive diagnosis w...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.19.6.952-953.1984
更新日期:1984-06-01 00:00:00
abstract::A Campylobacter-like organism was isolated from the ilea of normal hamsters. The organism was isolated from an ileal homogenate which was passed through a filter (0.65-micron pore size) and cultured on blood-agar plates in a microaerophilic atmosphere at 37 degrees C. Pinpoint translucent colonies were first observed ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.27.11.2497-2501.1989
更新日期:1989-11-01 00:00:00
abstract::The cellular fatty acid composition of Brucella canis and Brucella suis was determined by gas-liquid chromatography. The presence of relatively large amounts of a 19-carbon cyclopropane fatty acid in B. suis was a major distinguishing feature between these organisms. The gas-liquid chromatography test for cellular fat...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.14.1.111-112.1981
更新日期:1981-07-01 00:00:00
abstract::Despite attempts to control avian mycoplasmosis through management, vaccination, and surveillance, Mycoplasma gallisepticum continues to cause significant morbidity, mortality, and economic losses in poultry production. Live attenuated vaccines are commonly used in the poultry industry to control avian mycoplasmosis; ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00833-16
更新日期:2016-12-28 00:00:00
abstract::The impact of the viability of Trichomonas vaginalis in urine on wet mount, culture, and PCR methods was assessed. To minimize the chance of false-negative results, urine specimens should be processed within 30 min of specimen collection and maintained at 37 degrees C, since temperature appears to affect the viability...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01287-06
更新日期:2006-10-01 00:00:00
abstract::A combined clinical and molecular epidemiologic analysis of 46 strains of Clostridium difficile, including 16 nosocomial isolates from one ward (outbreak ward) plus 17 other nosocomial isolates and 13 community-acquired isolates, was performed. HindIII digests of total cellular DNA were analyzed by restriction enzyme ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.32.8.1963-1969.1994
更新日期:1994-08-01 00:00:00
abstract::The G and P types of 2,912 rotavirus-positive fecal specimens collected from eight geographical areas of the United Kingdom between 1995 and 1998 were determined by reverse transcription-PCR. Although 15 different G-P combinations were identified, G1P[8], G2P[4], G3P[8], and G4P[8] strains constituted 95% of all the r...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.38.12.4394-4401.2000
更新日期:2000-12-01 00:00:00
abstract::Legionella pneumophila, the causative agent of Legionnaires disease, and related organisms have previously been characterized primarily by conventional bacteriological methods, DNA-DNA hybridization, antigenic analysis, and fatty acid analysis. By capillary gas chromatographic analysis for carbohydrates, we have shown...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.19.3.326-332.1984
更新日期:1984-03-01 00:00:00
abstract::The staphylococcal methicillin resistance determinant, mecA, resides on a mobile genetic element, staphylococcus chromosomal cassette mec (SCCmec). The distribution of SCCmec in nature is limited to relatively few clonal complexes of related methicillin-resistant Staphylococcus aureus (MRSA). We have previously report...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.43.5.2380-2383.2005
更新日期:2005-05-01 00:00:00