Multicentric epidemiological study of Aspergillus fumigatus isolates by multilocus enzyme electrophoresis.

abstract：:We evaluated the clinical usefulness of species identification of blood isolates of coagulase-negative staphylococci as a predictor of the clinical significance of the isolates. In addition, we compared results of species identification obtained with MicroScan Rapid Gram-Positive Identification panels and Dried Overni...

journal_title：Journal of clinical microbiology

authors： Weinstein MP,Mirrett S,Van Pelt L,McKinnon M,Zimmer BL,Kloos W,Reller LB

更新日期：1998-07-01 00:00:00

abstract：:This study involved the rapid, direct identification of Staphylococcus aureus, Streptococcus pneumoniae, Escherichia coli, Haemophilus influenzae, Enterococcus sp., and Streptococcus agalactiae from positive blood culture bottles (BACTEC, Johnston Laboratories, Inc.) by using the AccuProbe (Gen-Probe, San Diego, Calif...

journal_title：Journal of clinical microbiology

authors： Davis TE,Fuller DD

更新日期：1991-10-01 00:00:00

abstract：:Ninety-four pharyngeal swab samples corresponding to 94 patients with suspected influenza virus infection were inoculated in Madin-Darby canine kidney (MDCK) cells, the conventional cell system for the isolation of influenza virus, and in fibroblastic human embryo lung (MRC-5) cells, a cell system less commonly used f...

journal_title：Journal of clinical microbiology

authors： de Oña M,Melón S,de la Iglesia P,Hidalgo F,Verdugo AF

更新日期：1995-07-01 00:00:00

abstract：:Zika virus (ZIKV) is a mosquito-borne flavivirus that is responsible for recent explosive epidemics in the Americas. Notably, ZIKV infection during pregnancy has been found to cause congenital birth defects, including microcephaly, and ZIKV has been associated with Guillain-Barré syndrome in adults. Diagnosis and surv...

journal_title：Journal of clinical microbiology

authors： Balmaseda A,Zambrana JV,Collado D,García N,Saborío S,Elizondo D,Mercado JC,Gonzalez K,Cerpas C,Nuñez A,Corti D,Waggoner JJ,Kuan G,Burger-Calderon R,Harris E

更新日期：2018-02-22 00:00:00

abstract：:Colistin-nalidixic acid agar, although recently recommended as a replacement for blood agar for primary plating of urine specimens ( Fung et al., J. Clin. Microbiol. 16:632-636, 1982), has also been reported to suppress the growth of some strains of staphylococci that are susceptible to colistin (polymyxin E). The sus...

journal_title：Journal of clinical microbiology

authors： Fung JC,McKinley G,Tyburski MB,Berman M,Goldstein J

更新日期：1984-05-01 00:00:00

abstract：:Phoenix 100 and Vitek 2 (operating with the current colorimetric cards) are commonly used in hospital laboratories for rapid identification of microorganisms. The present meta-analysis aims to evaluate and compare their performance on Gram-positive and Gram-negative bacteria. The MEDLINE database was searched up to Oc...

journal_title：Journal of clinical microbiology

authors： Chatzigeorgiou KS,Sergentanis TN,Tsiodras S,Hamodrakas SJ,Bagos PG

更新日期：2011-09-01 00:00:00

abstract：:The utility of combined 16S rRNA (rrs) gene restriction fragment length polymorphism and plasmid profiles to differentiate between and within Campylobacter upsaliensis of human and canine origin was examined. Fourteen distinct rrs gene restriction fragment length polymorphs consisting of bands sized between 1.9 and 4....

journal_title：Journal of clinical microbiology

authors： Stanley J,Jones C,Burnens A,Owen RJ

更新日期：1994-07-01 00:00:00

abstract：:In accordance with recent WHO recommendations, this study evaluates the sensitivities of PCR and microscopy for fine-needle aspiration (FNA) versus techniques involving swabs and punch biopsy specimens and suggests that FNA can replace punch biopsies for nonulcerative lesions and may serve as an alternative for ulcera...

journal_title：Journal of clinical microbiology

authors： Herbinger KH,Beissner M,Huber K,Awua-Boateng NY,Nitschke J,Thompson W,Klutse E,Agbenorku P,Assiobo A,Piten E,Wiedemann F,Fleischmann E,Helfrich K,Adjei O,Löscher T,Bretzel G

更新日期：2010-10-01 00:00:00

abstract：:One of the capabilities developed by bacteria is the ability to gain large fragments of DNA from other bacteria or to lose portions of their own genomes. Among these exchangeable fragments are the genomic islands (GIs). Nine GIs have been identified in Brucella, and genomic island 3 (GI-3) is shared by two pathogenic ...

journal_title：Journal of clinical microbiology

authors： Céspedes S,Salgado P,Valenzuela P,Vidal R,Oñate AA

更新日期：2011-07-01 00:00:00

abstract：:Human and porcine enterotoxigenic strains of Escherichia coli were cultivated in tryptone-yeast extract medium or brain heart infusion broth and tested for production of heat-labile enterotoxin by the GM1 ganglioside enzyme-linked immunosorbent assay (GM1-ELISA) and the Y1 adrenal cell assay. When testing for enteroto...

journal_title：Journal of clinical microbiology

authors： Gustafsson B,Möllby R

更新日期：1982-02-01 00:00:00

abstract：:A PCR specific for spacer regions 33 and 34 of the direct repeat region of the Mycobacterium tuberculosis complex was developed to complement the biochemical differentiation of M. tuberculosis, Mycobacterium bovis, M. bovis BCG, and Mycobacterium africanum subtypes I and II. In addition, this approach was incorporated...

journal_title：Journal of clinical microbiology

authors： Yeboah-Manu D,Yates MD,Wilson SM

更新日期：2001-11-01 00:00:00

abstract：:While testing the in vitro activities of 14 antimicrobial agents against 107 methicillin-susceptible Staphylococcus aureus (MSSA) and 250 methicillin-resistant S. aureus (MRSA) isolates collected in The Netherlands, we found to our surprise that 19 (7.6%) MRSA isolates were suspected of having reduced susceptibilities...

journal_title：Journal of clinical microbiology

authors： Van Griethuysen A,Van 't Veen A,Buiting A,Walsh T,Kluytmans J

更新日期：2003-06-01 00:00:00

abstract：:Two Hoffmann-La Roche products were evaluated in parallel for their ability to identify 321 strains of the family Enterobacteriaceae. The first product was the well-established Enterotube II, which correctly identified 90% of the isolates after 24 h of incubation. The second was the ID-E rotor, originally designed for...

journal_title：Journal of clinical microbiology

authors： Holmes B

更新日期：1989-05-01 00:00:00

abstract：:A hemolysin produced by Aeromonas hydrophila CA-11, isolated from an environmental source, was purified by sulfopropyl-Sephadex C-25 chromatography at pH 5.0. This hemolysin caused fluid accumulation in infant mouse intestines and rabbit intestinal loops and killed Vero cells, as did the hemolysin produced by strain A...

journal_title：Journal of clinical microbiology

authors： Asao T,Kozaki S,Kato K,Kinoshita Y,Otsu K,Uemura T,Sakaguchi G

更新日期：1986-08-01 00:00:00

abstract：:Whole-cell sonicates of Treponema pallidum, Nichols strain, were evaluated in an enzyme-linked immunosorbent assay (Elisa) for syphilis, and results were read in a Dynatek Microelisa Reader. The antigen was evaluated with sera from patients with syphilis, persons presumed normal, and biological false-positives. Two hu...

journal_title：Journal of clinical microbiology

authors： Pope V,Hunter EF,Feeley JC

更新日期：1982-04-01 00:00:00

abstract：:The cobas human papillomavirus (HPV) test, approved by the FDA in April 2011, is a fully automated assay for the detection of 14 high-risk (hr) HPV genotypes from cervical specimens collected in liquid-based cytology medium using real-time PCR amplification of the L1 gene and TaqMan probes. Results are simultaneously ...

journal_title：Journal of clinical microbiology

authors： Rao A,Young S,Erlich H,Boyle S,Krevolin M,Sun R,Apple R,Behrens C

更新日期：2013-05-01 00:00:00

abstract：:The contamination of semiclosed disposable circuits of Healthdyne and Bourns ventilators was studied in a newborn intensive care unit over a 2-year period. A total of 379 fluid samples was obtained from inspiratory and expiratory tubing condensates and traps and from thermal humidifier columns fed with prefilled conta...

journal_title：Journal of clinical microbiology

authors： Malecka-Griggs B

更新日期：1986-02-01 00:00:00

abstract：:Botulism developed in a patient following surgical repair of an open radial fracture. Symptoms resolved after treatment with antitoxin and antibiotics, and hardware excision was deferred. Subsequent osteomyelitis necessitated hardware exchange, and wound cultures grew Clostridium argentinense. This case highlights the...

journal_title：Journal of clinical microbiology

authors： Taylor SM,Wolfe CR,Dixon TC,Ruch DS,Cox GM

更新日期：2010-02-01 00:00:00

abstract：:Bacteriuria due to Salmonella typhi usually occurs following recent typhoid fever or in chronic carrier states. Data from 18 patients with S. typhi bacteriuria, seen during 5 years, were analyzed. Fourteen patients had localized urinary tract infection due to S. typhi. Four others had bacteriuria, probably associated ...

journal_title：Journal of clinical microbiology

authors： Mathai E,John TJ,Rani M,Mathai D,Chacko N,Nath V,Cherian AM

更新日期：1995-07-01 00:00:00

abstract：:A high-performance liquid chromatography method that utilized fluorescence detection (HPLC-FL) of mycolic acid 6,7-dimethoxycoumarin esters was developed to identify Mycobacterium tuberculosis (MTB) and M. avium complex (MAC) directly from fluorochrome stain smear-positive sputum specimens and young BACTEC 12B culture...

journal_title：Journal of clinical microbiology

authors： Jost KC Jr,Dunbar DF,Barth SS,Headley VL,Elliott LB

更新日期：1995-05-01 00:00:00

abstract：:The use of counterimmunoelectrophoresis (CIE) for detection of serum antibodies to staphylococcal teichoic acids was evaluated against teichoic acids prepared by sonic treatment or lysostaphin extraction of Staphylococcus aureus (Lafferty strain). Of 54 patient sera from suspected cases of staphylococcal endocarditis,...

journal_title：Journal of clinical microbiology

authors： Leffell MS,Folds JD,Wasilauskas B

更新日期：1978-11-01 00:00:00

abstract：:A latex agglutination test was evaluated as a method for detection and titration of antiplague antibodies. Slide and microtiter techniques using polystyrene latex particles coated with specific fraction-I-antigen of Yersinia pestis were found to be comparable in specificity and sensitivity to serological tests commonl...

journal_title：Journal of clinical microbiology

authors： Suzuki S,Sakakibara H,Hotta S

更新日期：1977-10-01 00:00:00

abstract：:Baculovirus-expressed foot-and-mouth disease virus (FMDV) nonstructural protein 3AB was used as the antigen in an enzyme-linked immunosorbent assay. This assay allowed the differentiation of vaccinated from infected pigs. Serial studies were performed using sera collected from pigs in the field. Positive reactions wer...

journal_title：Journal of clinical microbiology

authors： Chung WB,Sorensen KJ,Liao PC,Yang PC,Jong MH

更新日期：2002-08-01 00:00:00

abstract：:Two types of commercially available microdilution trays were inoculated with log-phase cultures, stationary-phase cultures, and direct suspensions of 18- to 24-h colonies, with essentially comparable results. Direct pick-up of colonies was also tested with two Prompt inoculation systems (3M Co.); they were found to be...

journal_title：Journal of clinical microbiology

authors： Barry AL,Badal RE,Hawkinson RW

更新日期：1983-09-01 00:00:00

abstract：:Shotgun proteomics using liquid chromatography-tandem mass spectrometry (LC-MS/MS) was applied to detect β-lactamases in clinical Acinetobacter baumannii isolates. The correlation of the detection of β-lactamase proteins (rather than PCR detection of the corresponding genes) with the resistance phenotypes demonstrated...

journal_title：Journal of clinical microbiology

authors： Trip H,Mende K,Majchrzykiewicz-Koehorst JA,Sedee NJ,Hulst AG,Jansen HJ,Murray CK,Paauw A

更新日期：2015-06-01 00:00:00

abstract：:Resistant cells of Pseudomonas aeruginosa ATCC 9027 which were grown in the presence of 1 mg of benzalkonium chloride (BC) per ml caused only a mild conjunctivitis when they were dropped onto the scratched corneas of rabbits. In contrast, cells of the BC-sensitive parent strain induced a severe keratoconjunctivitis. I...

journal_title：Journal of clinical microbiology

authors： Adair FW,Liauw HL,Geftic SG,Gelzer J

更新日期：1975-02-01 00:00:00

abstract：:Testing for susceptibility to pyrazinamide (PZA) and analysis of the pncA gene sequences of 423 Mycobacterium tuberculosis complex isolates have revealed a unique silent nucleotide substitution that enables the rapid identification of "M. canettii" (proposed name). Moreover, the lack of a defined mutation within the p...

journal_title：Journal of clinical microbiology

authors： Somoskovi A,Dormandy J,Parsons LM,Kaswa M,Goh KS,Rastogi N,Salfinger M

更新日期：2007-02-01 00:00:00

abstract：:We recently reported an outbreak of invasive aspergillosis in the major heart surgery unit of Hospital Gregorio Marañón, Madrid, Spain (T. Peláez, P. Muñoz, J. Guinea, M. Valerio, M. Giannella, C. H. W. Klaassen, and E. Bouza, Clin. Infect. Dis., in press). Aspergillus fumigatus was isolated from clinical samples from...

journal_title：Journal of clinical microbiology

authors： Guinea J,García de Viedma D,Peláez T,Escribano P,Muñoz P,Meis JF,Klaassen CH,Bouza E

更新日期：2011-10-01 00:00:00

abstract：:The performance of the Etest for voriconazole and for itraconazole susceptibility testing of 376 isolates of Aspergillus spp. was assessed in comparison with the National Committee for Clinical Laboratory Standards (NCCLS) proposed standard microdilution broth method. The NCCLS method employed RPMI 1640 broth medium, ...

journal_title：Journal of clinical microbiology

authors： Pfaller JB,Messer SA,Hollis RJ,Diekema DJ,Pfaller MA

更新日期：2003-03-01 00:00:00

abstract：:Members of the genus Abiotrophia, formerly known as nutritionally variant streptococci, are important pathogens causing septicemia and endocarditis. Cultivation and biochemical differentiation of Abiotrophia spp. are often difficult. Based on 16S rRNA sequences, two PCR assays for detection and identification of Abiot...

journal_title：Journal of clinical microbiology

authors： Roggenkamp A,Leitritz L,Baus K,Falsen E,Heesemann J

更新日期：1998-10-01 00:00:00