Isolation of a Campylobacter-like organism from healthy Syrian hamsters (Mesocricetus auratus).

Abstract:

:A Campylobacter-like organism was isolated from the ilea of normal hamsters. The organism was isolated from an ileal homogenate which was passed through a filter (0.65-micron pore size) and cultured on blood-agar plates in a microaerophilic atmosphere at 37 degrees C. Pinpoint translucent colonies were first observed after 120 h of incubation. The isolated organism measured 2.0 to 3.5 microns in length (excluding flagella) by 0.17 to 0.25 micron in width and typically had a single terminal sheathed flagellum. The organism was oxidase, catalase, and urease positive, reduced nitrates, and was susceptible to nalidixic acid (30-micrograms disk) and resistant to cephalothin (30-micrograms disk). Unlike Campylobacter pylori subsp. mustelae, this organism did not hydrolyze indoxylacetate. Immunofluorescence studies with a Campylobacter species-specific monoclonal antibody (8322-2E6) revealed the presence of numerous positively stained organisms within the crypt epithelial cells of the hamsters from which this organism was isolated. The role of this organism in the pathogenesis of proliferative ileitis in hamsters is uncertain, as is the taxonomic relationship of this organism to other members of the genus Campylobacter.

journal_name

J Clin Microbiol

authors

Stills HF Jr,Hook RR Jr,Kinden DA

doi

10.1128/JCM.27.11.2497-2501.1989

subject

Has Abstract

pub_date

1989-11-01 00:00:00

pages

2497-501

issue

11

eissn

0095-1137

issn

1098-660X

journal_volume

27

pub_type

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