Isolation of enterotoxigenic Bacteroides fragilis from Bangladeshi children with diarrhea: a controlled study.

abstract：:Molecular typing has become indispensable in the detection of nosocomial transmission of bacterial pathogens and the identification of sources and routes of transmission in outbreak settings, but current methods are labor-intensive, are difficult to standardize, or have limited resolution. Whole-genome multilocus sequ...

journal_title：Journal of clinical microbiology

authors： Kluytmans-van den Bergh MF,Rossen JW,Bruijning-Verhagen PC,Bonten MJ,Friedrich AW,Vandenbroucke-Grauls CM,Willems RJ,Kluytmans JA

更新日期：2016-12-01 00:00:00

abstract：:A large pool of refined endotoxin was prepared from Escherichia coli O113 by extraction with hot acqueous phenol. It was characterized chemically and biologically and will be available for a reference standard designated as reference endotoxin EC. ...

journal_title：Journal of clinical microbiology

authors： Rudbach JA,Akiya FI,Elin RJ,Hochstein HD,Luoma MK,Milner EC,Milner KC,Thomas KR

更新日期：1976-01-01 00:00:00

abstract：:Typical and atypical Campylobacter strains were isolated from the colons of cattle and swine by techniques that enabled the selective pressures of antibiotics to be avoided. Some cephalothin-susceptible strains and a strain with an indeterminate hippurate reaction were classified as Campylobacter coli by DNA homology ...

journal_title：Journal of clinical microbiology

authors： Ng LK,Taylor DE,Stiles ME

更新日期：1988-03-01 00:00:00

abstract：:The usefulness of filter paper for preservation of bacterial cells was shown by mixed-linker DNA fingerprint analysis of Mycobacterium tuberculosis isolates from 77 Brazilian patients. DNA fingerprints of samples spotted onto filter paper and conventional culture material were identical. Thus, filter paper specimens a...

journal_title：Journal of clinical microbiology

authors： Burger M,Raskin S,Brockelt SR,Amthor B,Geiss HK,Haas WH

更新日期：1998-02-01 00:00:00

abstract：:In the present study, we compared typed F. nucleatum strains with isolates from various human periodontal diseases, canines, and a Macaca mulatta monkey. All isolates displayed biochemical reactions similar to those of the typed strains in the API 20A system (Analytab Products, Plainville, N.Y.). The human and monkey ...

journal_title：Journal of clinical microbiology

authors： Vincent JW,Falkler WA Jr,Craig JA

更新日期：1983-04-01 00:00:00

abstract：:We studied whether the Etest can be used as an alternative to agar dilution to determine antimicrobial susceptibilities of ceftriaxone, cefixime, and cefpodoxime in Neisseria gonorrhoeae surveillance. One hundred fifteen clinical and laboratory isolates of N. gonorrhoeae were tested following the Clinical Laboratory I...

journal_title：Journal of clinical microbiology

authors： Liu H,Taylor TH Jr,Pettus K,Trees D

更新日期：2014-05-01 00:00:00

abstract：:A comparison of three different commercial media was made to assess their recovery of anaerobic organisms from the blood stream. The three media used were the 50-ml brain heart infusion broth with added CO2 (Pfizer), the 50-ml Thiol broth with added CO2 (Difco), and the 50-ml prereduced, supplemented peptone broth in ...

journal_title：Journal of clinical microbiology

authors： Mangels JI,Lindberg LH,Vosti KL

更新日期：1977-05-01 00:00:00

abstract：:Escherichia coli isolates that cause detachment of cell monolayers during in vitro adherence assays (cell-detaching E. coli [CDEC]) were recently reported as a potential new group of enteropathogenic bacteria. In the present study, 269 E. coli isolates from feces of children 1 to 5 years of age were identified as CDEC...

journal_title：Journal of clinical microbiology

authors： Marques LR,Abe CM,Griffin PM,Gomes TA

更新日期：1995-10-01 00:00:00

abstract：:A total of 309 Helicobacter pylori isolates from 18 different countries were analyzed with a previously developed lectin typing system. The system was developed by using a proteolytic pretreatment to enhance the carbohydrate fraction of the sample. Four lectins from Ulex europaeus, Lotus tetragonolobus, Erythrina cris...

journal_title：Journal of clinical microbiology

authors： Hynes SO,Broutet N,Wadström T,Mikelsaar M,O'Toole PW,Telford J,Engstrand L,Kamiya S,Mentis AF,Moran AP,Eurohepygast Study Group.

更新日期：2002-01-01 00:00:00

abstract：:Streptococcus uberis and Streptococcus parauberis reference strains and isolates obtained from routine diagnostics were investigated by PCR with oligonucleotide primers designed according to species-specific parts of the 16S rRNA gene, the 23S rRNA gene, and the 16S-23S rRNA intergenic spacer region of both species. A...

journal_title：Journal of clinical microbiology

authors： Hassan AA,Khan IU,Abdulmawjood A,Lämmler C

更新日期：2001-04-01 00:00:00

abstract：:Mycobacteria include a large number of pathogens. Identification to species level is important for diagnoses and treatments. Here, we report the development of a Web-accessible database of the hsp65 locus sequences (http://msis.mycobacteria.info) from 149 out of 150 Mycobacterium species/subspecies. This database can ...

journal_title：Journal of clinical microbiology

authors： Dai J,Chen Y,Lauzardo M

更新日期：2011-06-01 00:00:00

abstract：:Infection is an important complication in patients with hematologic malignancies or solid tumors undergoing intensive cytotoxic chemotherapy. In only 20 to 30% of the febrile neutropenic episodes, an infectious agent is detected by conventional cultures. In this prospective study, the performance of broad-range PCR co...

journal_title：Journal of clinical microbiology

authors： Desmet S,Maertens J,Bueselinck K,Lagrou K

更新日期：2016-10-01 00:00:00

abstract：:A new immunochromatographic rapid test, Determine HIV-1/2, for the detection of antibodies to human immunodeficiency virus type 1 (HIV-1) and HIV-2 in human whole blood, serum, and plasma was evaluated. Determine HIV-1/2 is a sandwich immunoassay and uses a nitrocellulose strip with a capture site for the patient's re...

journal_title：Journal of clinical microbiology

authors： Arai H,Petchclai B,Khupulsup K,Kurimura T,Takeda K

更新日期：1999-02-01 00:00:00

abstract：:Restriction fragment length polymorphism (RFLP) analysis with probes derived from the insertion element IS6110, the direct repeat sequence, and the polymorphic GC-rich sequence (PGRS) and a PCR-based typing method called spacer oligonucleotide typing (spoligotyping) were used to strain type Mycobacterium bovis isolate...

journal_title：Journal of clinical microbiology

authors： Costello E,O'Grady D,Flynn O,O'Brien R,Rogers M,Quigley F,Egan J,Griffin J

更新日期：1999-10-01 00:00:00

abstract：:Yersinia pestis is the causative agent of plague and is considered a category A priority pathogen due to its potential for high transmissibility and the significant morbidity and mortality it causes in humans. Y. pestis is endemic to the western United States and much of the world, necessitating programs to monitor fo...

journal_title：Journal of clinical microbiology

authors： Chandler JC,Baeten LA,Griffin DL,Gidlewski T,DeLiberto TJ,Petersen JM,Pappert R,Young JW,Bevins SN

更新日期：2018-06-25 00:00:00

abstract：:Viridans streptococci (VS) are common etiologic agents of subacute infective endocarditis and are capable of causing a variety of pyogenic infections. Many species of VS are difficult to differentiate by phenotypic traits. An oligonucleotide array based on 16S-23S rRNA gene intergenic spacer (ITS) sequences was develo...

journal_title：Journal of clinical microbiology

authors： Chen CC,Teng LJ,Kaiung S,Chang TC

更新日期：2005-04-01 00:00:00

abstract：:An enzyme immunoassay (EIA) for serum immunoglobulin M (IgM) antibodies to rubella virus based on enzyme labeling of viral antigen was developed. The sensitivity of the EIA for the detection of recent rubella virus infection was evaluated by using 115 rubella-IgM-antibody-positive serum specimens, which were confirmed...

journal_title：Journal of clinical microbiology

authors： Seppänen H

更新日期：1990-04-01 00:00:00

abstract：:The longus type IV pilus structural gene (lngA) was sought among 217 clinical enterotoxigenic Escherichia coli (ETEC) strains isolated in Argentina. lngA was present in 20.7% of the isolates and was highly associated with ETEC producing heat-stable toxin and the most common colonization factors. The prevalence of long...

journal_title：Journal of clinical microbiology

authors： Pichel MG,Binsztein N,Qadri F,Girón JA

更新日期：2002-02-01 00:00:00

abstract：:Naturally acquired infection of humans with a marine mammal-associated Brucella sp. has only been reported once previously in a study describing infections of two patients from Peru. We report the isolation and characterization of a strain of Brucella from a New Zealand patient that appears most closely related to str...

journal_title：Journal of clinical microbiology

authors： McDonald WL,Jamaludin R,Mackereth G,Hansen M,Humphrey S,Short P,Taylor T,Swingler J,Dawson CE,Whatmore AM,Stubberfield E,Perrett LL,Simmons G

更新日期：2006-12-01 00:00:00

abstract：:Multidrug-resistant tuberculosis is an increasing health problem worldwide, especially in developing countries. The PCR-UHG-Rif assay, which detects mutations within the rpoB gene associated with rifampin resistance, was evaluated for its ability and reliability to detect and identify drug-resistant Mycobacterium tube...

journal_title：Journal of clinical microbiology

authors： Mayta H,Gilman RH,Arenas F,Valencia T,Caviedes L,Montenegro SH,Ticona E,Ortiz J,Chumpitaz R,Evans CA,Williams DL,Tuberculosis Working Group in Perú.

更新日期：2003-12-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay method was used to classify bovine serum as positive, negative, or doubtful for antibodies to Brucella abortus. Spectrophotometric data from assays of 64 serologically positive and 32 serologically negative bovine sera were analyzed statistically to define the range of spectrophoto...

journal_title：Journal of clinical microbiology

authors： Heck FC,Williams JD,Pruett J

更新日期：1980-04-01 00:00:00

abstract：:The genetic composition and antibiotic sensitivities of 50 clinical isolates of Staphylococcus aureus obtained from various clinics in the Republic of Georgia were characterized. S. aureus strains ATCC 700699 and ATCC 29737 were included as reference standards in all analyses. All 52 strains had identical 16S rRNA pro...

journal_title：Journal of clinical microbiology

authors： Revazishvili T,Bakanidze L,Gomelauri T,Zhgenti E,Chanturia G,Kekelidze M,Rajanna C,Kreger A,Sulakvelidze A

更新日期：2006-10-01 00:00:00

abstract：:To simplify the serotyping of Streptococcus pneumoniae, a dot bot assay has been developed and compared with the standard quellung reaction in 1,082 isolates. The technique has been demonstrated to be sensitive, specific, easy to perform, and inexpensive. The dot blot assay could be useful when large numbers of pneumo...

journal_title：Journal of clinical microbiology

authors： Fenoll A,Jado I,Vicioso D,Casal J

更新日期：1997-03-01 00:00:00

abstract：:The studies reported here were undertaken to assess the ability of the outer membrane proteins (OMPs) of Salmonella typhi to induce a humoral immune response in humans with typhoid fever. OMPs were isolated with the nonionic detergent Triton X-100 and were found to be contaminated with approximately 4% lipopolysacchar...

journal_title：Journal of clinical microbiology

authors： Ortiz V,Isibasi A,García-Ortigoza E,Kumate J

更新日期：1989-07-01 00:00:00

abstract：:Pulsed-field gel electrophoresis revealed that multiple consecutive isolates of Legionella pneumophila from two cardiac transplant patients remained genomically stable, despite exposure to host defenses and antimicrobial agents. ...

journal_title：Journal of clinical microbiology

authors： Marrie TJ,Johnson WM,Tyler SD,Bezanson GS,Burbridge S

更新日期：1994-12-01 00:00:00

abstract：:Many parts of the Salmonella rfb gene clusters which are responsible for biosynthesis of the oligosaccharide-repeating units of the O-antigenic lipopolysaccharide have recently been cloned and sequenced. On the basis of this knowledge, three sets of nucleotide primers were selected to target defined regions of the abe...

journal_title：Journal of clinical microbiology

authors： Luk JM,Kongmuang U,Reeves PR,Lindberg AA

更新日期：1993-08-01 00:00:00

abstract：:An unusual Enterocytozoon bieneusi genotype was found in seven guinea pigs and a 2-year-old child in the same household. The genetic uniqueness of the parasite, its wide occurrence in other guinea pigs in the community, and its absence in other children in the community suggest the possibility of zoonotic transmission...

journal_title：Journal of clinical microbiology

authors： Cama VA,Pearson J,Cabrera L,Pacheco L,Gilman R,Meyer S,Ortega Y,Xiao L

更新日期：2007-08-01 00:00:00

abstract：:Depletion of swabs and viral transport medium during epidemics may prompt the use of unvalidated alternatives. Swabs collected and transported dry or in saline were compared to commercially available swab/medium combinations for PCR detection of influenza, enterovirus, herpes simplex virus, and adenovirus. Each was de...

journal_title：Journal of clinical microbiology

authors： Druce J,Garcia K,Tran T,Papadakis G,Birch C

更新日期：2012-03-01 00:00:00

abstract：:Direct inoculation onto Granada medium (GM) in plates and tubes was compared to inoculation into a selective Todd-Hewitt broth (with 8 microg of gentamicin per ml and 15 microg of nalidixic acid per ml) for detection of group B streptococci (GBS) in pregnant women with 800 vaginal and 450 vaginoanorectal samples. Comp...

journal_title：Journal of clinical microbiology

authors： Rosa-Fraile M,Rodriguez-Granger J,Cueto-Lopez M,Sampedro A,Gaye EB,Haro JM,Andreu A

更新日期：1999-08-01 00:00:00

abstract：:Sixty rotavirus-positive stool specimens from children with diarrhea were classified into G and P genotypes. G typing was done by PCR and then by hybridization with G type-specific (G1 to G4) oligonucleotide probes, whereas nested PCR was performed for P typing. Thirty-nine samples could be classified into both G and ...

journal_title：Journal of clinical microbiology

authors： Husain M,Seth P,Dar L,Broor S

更新日期：1996-06-01 00:00:00