Characterization of a Brucella sp. strain as a marine-mammal type despite isolation from a patient with spinal osteomyelitis in New Zealand.

abstract：:An assay to detect and sequence DNA from human cytomegalovirus (HCMV) immediate-early gene region 1 has been developed; it involves in vitro amplification by the polymerase chain reaction and direct solid-phase sequencing of the amplified material. Urine samples from 16 patients tested positive for HCMV DNA in both a ...

journal_title：Journal of clinical microbiology

authors： Brytting M,Wahlberg J,Lundeberg J,Wahren B,Uhlén M,Sundqvist VA

更新日期：1992-04-01 00:00:00

abstract：:Two hundred and twelve Micrococcaceae isolates were obtained from 82 men with nongonococcal urethritis, 24 women with vaginitis, and 54 girls with vulvovaginitis. Identification and biotyping of these strains were carried out by using the simplified scheme of Kloos and Schleifer (W. E. Kloos and K. H. Schleifer, J. Cl...

journal_title：Journal of clinical microbiology

authors： Baldellon C,Mégraud F

更新日期：1985-03-01 00:00:00

abstract：:Endotoxin concentrations were determined in middle-ear effusions (MEEs) from 89 children with chronic otitis media by using the Limulu's amoebocyte lysate assay. Mean concentrations of endotoxin in Haemophilus influenzae-positive and Streptococcus pneumoniae-positive MEEs were 157 and 21.8 ng/ml, respectively, and wer...

journal_title：Journal of clinical microbiology

authors： DeMaria TF,Prior RB,Briggs BR,Lim DJ,Birck HG

更新日期：1984-07-01 00:00:00

abstract：:We compared 14 molecular assays for their ability to detect the Mycobacterium tuberculosis complex in bronchoalveolar lavage fluid samples. Three approaches were followed. First, by using DNA from Mycobacterium bovis BCG, we determined the detection limits of the assays using routine molecular methods. Second, in orde...

journal_title：Journal of clinical microbiology

authors： Akkerman OW,van der Werf TS,de Boer M,de Beer JL,Rahim Z,Rossen JW,van Soolingen D,Kerstjens HA,van der Zanden AG

更新日期：2013-11-01 00:00:00

abstract：:In a prospective study, 139 serial blood samples from 15 transplant recipients were assessed for the presence of cytomegalovirus (CMV) by virus isolation (CMV viremia) and by direct staining of CMV antigens (CMV Ag) in blood leukocytes (CMV antigenemia). CMV was isolated from 23 samples, whereas CMV Ag was detected in...

journal_title：Journal of clinical microbiology

authors： van der Bij W,Schirm J,Torensma R,van Son WJ,Tegzess AM,The TH

更新日期：1988-12-01 00:00:00

abstract：:This study evaluated the feasibility of a molecular strategy based on identification of Mycobacterium tuberculosis by IS6110 PCR or Cobas Amplicor PCR, and rpoB PCR-DNA sequencing of the 81-bp rifampin resistance determining region (RRDR) for direct detection of rifampin resistance in respiratory specimens. A collecti...

journal_title：Journal of clinical microbiology

authors： Yam WC,Tam CM,Leung CC,Tong HL,Chan KH,Leung ET,Wong KC,Yew WW,Seto WH,Yuen KY,Ho PL

更新日期：2004-10-01 00:00:00

abstract：:The ImmunoCard STAT! Cryptosporidium/Giardia rapid assay (Meridian Bioscience, Inc.) is a solid-phase qualitative immunochromatographic assay that detects and distinguishes between Giardia lamblia and Cryptosporidium parvum in aqueous extracts of human fecal specimens (fresh, frozen, unfixed, or fixed in 5 or 10% form...

journal_title：Journal of clinical microbiology

authors： Garcia LS,Shimizu RY,Novak S,Carroll M,Chan F

更新日期：2003-01-01 00:00:00

abstract：:This paper describes the first isolation of a new Shigella flexneri serotype, designated 4s, in Beijing, China. Genotypic and phenotypic profiling suggests that this isolate is a clone of the S. flexneri serotype X variant reference strain. Of particular concern is the multidrug resistance exhibited by this isolate. ...

journal_title：Journal of clinical microbiology

authors： Qiu S,Wang Z,Chen C,Liu N,Jia L,Liu W,Wang L,Hao R,Zhang L,Wang Y,Song H

更新日期：2011-03-01 00:00:00

abstract：:The carbohydrate fermentation test in cystine trypticase agar (BBL)-tubed medium was compared with a simple method using commercially available carbohydrate impregnated disks on culture inoculated Thayer-Martin medium with and without (vancomycin, colistimethate, and nystatin) inhibitors. There was 100% agreement betw...

journal_title：Journal of clinical microbiology

authors： Valu JA

更新日期：1976-02-01 00:00:00

abstract：:The polymerase chain reaction (PCR) has many potential applications in the field of nucleic acid diagnostics. In particular, it has been successfully applied to the detection of pathogens present in low copy numbers such as the human immunodeficiency virus type 1. Here we describe a time-resolved fluorescence-based hy...

journal_title：Journal of clinical microbiology

authors： Dahlén PO,Iitiä AJ,Skagius G,Frostell A,Nunn MF,Kwiatkowski M

更新日期：1991-04-01 00:00:00

abstract：:HPV68a is not efficiently detected by PCR with the PGMY primers. Version 2 of the PGMY-CHUV assay (PGv2) was developed from version 1 (PGv1) to evaluate HPV68-discordant results with the Anyplex II HPV28 assay. We now report that PGv2 is significantly more sensitive than PGv1 for HPV68a and as sensitive and specific f...

journal_title：Journal of clinical microbiology

authors： Estrade C,Sahli R

更新日期：2014-11-01 00:00:00

abstract：:In an initial experiment, culture-grown Borrelia burgdorferi was added to freshly collected uninfected human blood. This in vitro study demonstrated that more spirochetes were distributed into the plasma than into the serum fraction. In a subsequent clinical study, B. burgdorferi was recovered from plasma cultures of ...

journal_title：Journal of clinical microbiology

authors： Wormser GP,Bittker S,Cooper D,Nowakowski J,Nadelman RB,Pavia C

更新日期：2000-04-01 00:00:00

abstract：:The aim of this study was to develop a PCR and reverse line blot hybridization (PCR-RLB) macroarray assay based on 16S-23S rRNA gene internal transcribed spacer sequences for the identification and differentiation of 34 mycobacterial species or subspecies. The performance of the PCR-RLB assay was assessed and validate...

journal_title：Journal of clinical microbiology

authors： Xiong L,Kong F,Yang Y,Cheng J,Gilbert GL

更新日期：2006-10-01 00:00:00

abstract：:Ribotyping was compared with multilocus enzyme electrophoresis (MEE) for subtyping 305 Listeria monocytogenes isolates from clinical and nonclinical sources. For ribotyping, EcoRI-restricted genomic DNA fragments of L. monocytogenes strains were separated by agarose gel electrophoresis, and Southern blots were probed ...

journal_title：Journal of clinical microbiology

authors： Graves LM,Swaminathan B,Reeves MW,Hunter SB,Weaver RE,Plikaytis BD,Schuchat A

更新日期：1994-12-01 00:00:00

abstract：:Fifty-six strains of Borrelia burgdorferi sensu lato, isolated from ticks and vertebrate animals in Missouri, South Carolina, Georgia, Florida, and Texas, were identified and characterized by PCR-restriction fragment length polymorphism (RFLP) analysis of rrf (5S)-rrl (23S) intergenic spacer amplicons. A total of 241 ...

journal_title：Journal of clinical microbiology

authors： Lin T,Oliver JH Jr,Gao L,Kollars TM Jr,Clark KL

更新日期：2001-07-01 00:00:00

abstract：:When the standard procedure for determining antibiotic susceptibility of bacteria is used, the results are delayed, especially for bacteria that grow slowly, such as Helicobacter pylori. Treatment for this bacterium may involve clarithromycin, a compound for which resistance has been associated with point mutations on...

journal_title：Journal of clinical microbiology

authors： Pina M,Occhialini A,Monteiro L,Doermann HP,Mégraud F

更新日期：1998-11-01 00:00:00

abstract：:In a multi-health center study, a new rapid optical immunoassay (OIA) for the detection of Shiga toxin types 1 and 2, the BioStar OIA SHIGATOX kit (Inverness Medical Professional Diagnostics, Inc.), was used to prospectively screen 742 fresh fecal samples for Shiga toxins in parallel with the Premier enterohemorrhagic...

journal_title：Journal of clinical microbiology

authors： Teel LD,Daly JA,Jerris RC,Maul D,Svanas G,O'Brien AD,Park CH

更新日期：2007-10-01 00:00:00

abstract：:Bacillus anthracis spores have been used as a biological weapon in the United States. We wanted to develop a safe, rapid method of sample preparation that provided safe DNA for the detection of spores in environmental and clinical specimens. Our method reproducibly detects B. anthracis in samples containing <10 spores...

journal_title：Journal of clinical microbiology

authors： Luna VA,King D,Davis C,Rycerz T,Ewert M,Cannons A,Amuso P,Cattani J

更新日期：2003-03-01 00:00:00

abstract：:Molecular types of the Cryptococcus neoformans/Cryptococcus gattii species complex that infect dogs and cats differ regionally and with host species. Antifungal drug susceptibility can vary with molecular type, but the susceptibility of Cryptococcus isolates from dogs and cats is largely unknown. Cryptococcus isolates...

journal_title：Journal of clinical microbiology

authors： Singer LM,Meyer W,Firacative C,Thompson GR 3rd,Samitz E,Sykes JE

更新日期：2014-06-01 00:00:00

abstract：:Infections with human immunodeficiency virus types 1 and 2 (HIV-1 and HIV-2, respectively) are zoonotic infections. In Africa, the potential exists for additional cross-species transmissions from at least 33 different species of simian immunodeficiency virus (SIV)-infected nonhuman primates (NHPs) through hunting and ...

journal_title：Journal of clinical microbiology

authors： Ndongmo CB,Switzer WM,Pau CP,Zeh C,Schaefer A,Pieniazek D,Folks TM,Kalish ML

更新日期：2004-11-01 00:00:00

abstract：:From January 1995 to May 1998, 57 episodes of bacteremia due to viridans group streptococci were identified in 50 febrile neutropenic patients with hematologic malignancies. Four patients experienced two separate episodes of streptococcal bacteremia, and one patient had four separate episodes of streptococcal bacterem...

journal_title：Journal of clinical microbiology

authors： Wisplinghoff H,Reinert RR,Cornely O,Seifert H

更新日期：1999-06-01 00:00:00

abstract：:In a prospective observational study of bacteremic patients we ascertained the influence of different parts of culture results on the correctness of empirical antibiotic therapy. Ninety-three bacteremic patients requiring antibiotic treatment were included. Patients who had consultations with an infectious disease con...

journal_title：Journal of clinical microbiology

authors： Kerremans JJ,Verbrugh HA,Vos MC

更新日期：2012-06-01 00:00:00

abstract：:A mucoid strain of Pseudomonas aeruginosa isolated from a patient with cystic fibrosis and its nonmucoid revertant were grown in a chemically defined alginate-promoting medium under batch and continuous culture conditions. Selection for the mucoid and nonmucoid phenotype was accomplished by varying the levels of air a...

journal_title：Journal of clinical microbiology

authors： Krieg DP,Bass JA,Mattingly SJ

更新日期：1986-12-01 00:00:00

abstract：:We reviewed laboratory-acquired infections occurring in Utah from 1978 through 1982. Written and telephone interviews of supervisors of 1,191 laboratorians revealed an estimated annual incidence of 3 laboratory-acquired infections per 1,000 employees. Infections, in order of frequency, included hepatitis B (clinical c...

journal_title：Journal of clinical microbiology

authors： Jacobson JT,Orlob RB,Clayton JL

更新日期：1985-04-01 00:00:00

abstract：:A European multicenter study was performed to evaluate the performance of a new method, based on the transcription-reverse transcription concerted reaction (TRC-2), which enabled one-step amplification and real-time detection of the Mycobacterium tuberculosis 16S rRNA target directly in clinical specimens. A total of ...

journal_title：Journal of clinical microbiology

authors： Drouillon V,Delogu G,Dettori G,Lagrange PH,Benecchi M,Houriez F,Baroli K,Ardito F,Torelli R,Chezzi C,Fadda G,Herrmann JL

更新日期：2009-11-01 00:00:00

abstract：:Rapid tests for detection of methicillin-resistant Staphylococcus aureus (MRSA) carriage are important to limit the transmission of MRSA in the health care setting. We evaluated the performance of the BD GeneOhm MRSA real-time PCR assay using a diverse collection of MRSA isolates, mainly from Copenhagen, Denmark, but ...

journal_title：Journal of clinical microbiology

authors： Bartels MD,Boye K,Rohde SM,Larsen AR,Torfs H,Bouchy P,Skov R,Westh H

更新日期：2009-05-01 00:00:00

abstract：:We investigated the potential of pooling DNA from nasopharyngeal specimens to reduce the cost of real-time PCR (RT-PCR) for bacterial detection. Lyophilization is required to reconcentrate DNA. This strategy yields a high specificity (86%) and a high sensitivity (96%). We estimate that compared to individual testing, ...

journal_title：Journal of clinical microbiology

authors： Edouard S,Prudent E,Gautret P,Memish ZA,Raoult D

更新日期：2015-03-01 00:00:00

abstract：:Nontuberculous mycobacterial lymphadenitis presents an increasing clinical problem in immunocompetent young children. A slowly growing, nonphotochromogenic mycobacterium was recovered twice (isolates 2553/91 and 2554/91) from the lymphatic tissue of a child with recurrent cervical lymphadenitis. It could be differenti...

journal_title：Journal of clinical microbiology

authors： Haas WH,Butler WR,Kirschner P,Plikaytis BB,Coyle MB,Amthor B,Steigerwalt AG,Brenner DJ,Salfinger M,Crawford JT,Böttger EC,Bremer HJ

更新日期：1997-12-01 00:00:00

abstract：:Clostridium difficile is the most common cause of health care-associated diarrhea. Accurate and rapid diagnosis is essential to improve patient outcome and prevent disease spread. We compared our two-step diagnostic algorithm, an enzyme immunoassay for glutamate dehydrogenase (GDH) followed by the cytotoxin neutraliza...

journal_title：Journal of clinical microbiology

authors： Babady NE,Stiles J,Ruggiero P,Khosa P,Huang D,Shuptar S,Kamboj M,Kiehn TE

更新日期：2010-12-01 00:00:00

abstract：:Commercially prepared fluorescein-labeled antihuman antibodies were labed with 125I and used to compre specific herpes simplex virus antibody titers as determined by indirect fluorescent antibody and radioimmunoassay techniques. Total virus-specific immunoglobulin and virus-specific immunoglobulin G titers did not var...

journal_title：Journal of clinical microbiology

authors： Parkinson AJ,Kalmakoff J

更新日期：1976-06-01 00:00:00