Use of PCR and reverse line blot hybridization macroarray based on 16S-23S rRNA gene internal transcribed spacer sequences for rapid identification of 34 mycobacterium species.

abstract：:Actinobacillus pleuropneumoniae strains of serotypes 1 and 9 were studied for their serological properties by means of agglutination, coagglutination (CoA), indirect hemagglutination (IHA), Co-IHA, ring precipitation (RP), and immunodiffusion (ID) tests. Particulate and soluble antigens of unheated and heat-treated ba...

journal_title：Journal of clinical microbiology

authors： Mittal KR

更新日期：1990-03-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay for antibodies to Borrelia burgdorferi in sheep was established. The assay was used in a seroepidemiological survey in which serum samples from 327 Norwegian rams were screened for antibodies to B. burgdorferi. These rams were randomly chosen, clinically healthy animals from all pa...

journal_title：Journal of clinical microbiology

authors： Fridriksdóttir V,Nesse LL,Gudding R

更新日期：1992-05-01 00:00:00

abstract：:We report a failure of the real-time reverse transcriptase PCR H7 subtyping protocol currently used in national avian influenza surveillance programs. Significant substitutions in primer and probe target sequences were identified, especially in wild bird viruses. The protocol, originally designed for detecting H7 infl...

journal_title：Journal of clinical microbiology

authors： Xing Z,Cardona C,Dao P,Crossley B,Hietala S,Boyce W

更新日期：2008-05-01 00:00:00

abstract：:We describe a rare case of Francisella novicida bacteremia following a near-drowning event in seawater. We highlight the challenges associated with laboratory identification of F. novicida and differences in the epidemiology of F. novicida and Francisella tularensis infections. ...

journal_title：Journal of clinical microbiology

authors： Brett M,Doppalapudi A,Respicio-Kingry LB,Myers D,Husband B,Pollard K,Mead P,Petersen JM,Whitener CJ

更新日期：2012-08-01 00:00:00

abstract：:The description and evaluation of a PCR-based assay for the detection and species identification of the eight known human herpesviruses are presented. Two primer pairs targeting well-conserved regions of the genome allowed the amplification of the DNAs of all known human herpesviruses at a high level of sensitivity (1...

journal_title：Journal of clinical microbiology

authors： Johnson G,Nelson S,Petric M,Tellier R

更新日期：2000-09-01 00:00:00

abstract：:Ten cases of typhoid fever occurred between 24 August and 1 September 1986 in the vicinity of Silver Spring, Md. Shrimp salad served in a fast-food restaurant was implicated as the source of infection. Stool cultures were obtained from 104 employees, and serum Vi antibodies were assayed in 97 of the employees. Salmone...

journal_title：Journal of clinical microbiology

authors： Lin FY,Becke JM,Groves C,Lim BP,Israel E,Becker EF,Helfrich RM,Swetter DS,Cramton T,Robbins JB

更新日期：1988-06-01 00:00:00

abstract：:A rapid and sensitive PCR assay for the detection of Candida albicans DNA in serum was established. DNA from human serum samples was purified using the QIAamp blood kit, which proved to be a fast and simple method for isolating minute amounts of Candida DNA from clinical specimens for diagnosis of invasive candidiasis...

journal_title：Journal of clinical microbiology

authors： Wahyuningsih R,Freisleben HJ,Sonntag HG,Schnitzler P

更新日期：2000-08-01 00:00:00

abstract：:Streptococcus agalactiae causes severe invasive disease in humans and mastitis in cattle. Temporally matched bovine milk isolates and clinical human invasive isolates (52 each) collected in New York State over 18 months were characterized by molecular subtyping and phenotypic methods to probe the interspecies transmis...

journal_title：Journal of clinical microbiology

authors： Sukhnanand S,Dogan B,Ayodele MO,Zadoks RN,Craver MP,Dumas NB,Schukken YH,Boor KJ,Wiedmann M

更新日期：2005-03-01 00:00:00

abstract：:The performance of three line blot assays (LBAs), the Linear Array HPV genotyping assay (LA) (Roche Diagnostics), INNO-LiPA HPV Genotyping Extra (LiPA) (Innogenetics), and the reverse hybridization assay (RH) (Qiagen), was evaluated using quantitated whole genomic human papillomavirus (HPV) plasmids (types 6, 11, 16, ...

journal_title：Journal of clinical microbiology

authors： Steinau M,Onyekwuluje JM,Scarbrough MZ,Unger ER,Dillner J,Zhou T

更新日期：2012-05-01 00:00:00

abstract：:A total of 513 blood specimens, predominantly from organ transplant recipients, human immunodeficiency virus-positive patients, and bone marrow transplant recipients, were tested for cytomegalovirus (CMV) by culture and pp65 antigenemia across four test sites. Peripheral blood leukocytes were examined by using both th...

journal_title：Journal of clinical microbiology

authors： St George K,Boyd MJ,Lipson SM,Ferguson D,Cartmell GF,Falk LH,Rinaldo CR,Landry ML

更新日期：2000-04-01 00:00:00

abstract：:A combined method of the Limulus amebocyte lysate (LAL) test and the enzyme-linked immunosorbent assay for quantitation of endotoxin was developed based on our observation that the antigenicity of coagulogen, a major protein in LAL, was lost when LAL reacted with endotoxin as shown by immunoblotting. Determination of ...

journal_title：Journal of clinical microbiology

authors： Zhang GH,Baek L,Koch C

更新日期：1988-08-01 00:00:00

abstract：:Forty human clinical Mycobacterium avium-M. intracellulare complex strains isolated in Greece were characterized to the species level by PCR with three sets of primers specific for one or both species. M. avium predominated in both human immunodeficiency virus-positive and -negative patients, but the frequency of M. i...

journal_title：Journal of clinical microbiology

authors： Kyriakopoulos AM,Tassios PT,Matsiota-Bernard P,Marinis E,Tsaousidou S,Legakis NJ

更新日期：1997-11-01 00:00:00

abstract：:In acute and chronic hepatitis B, antibodies of the immunoglobulin M (IgM) class against the hepatitis B core antigen (anti-HBc IgM) have been demonstrated. For the determination of anti-HBc IgM, a sensitive enzyme immunoassay with anti-mu-coated flat-bottomed microtiter plates is described and evaluated. The specific...

journal_title：Journal of clinical microbiology

authors： Roggendorf M,Deinhardt F,Frösner GG,Scheid R,Bayerl B,Zachoval R

更新日期：1981-04-01 00:00:00

abstract：:PCR-restriction fragment length polymorphism analysis (PRA) using the novel region of the rpoB gene was developed for rapid and precise identification of mycobacteria to the species level. A total of 50 mycobacterial reference strains and 3 related bacterial strains were used to amplify the 360-bp region of rpoB, and ...

journal_title：Journal of clinical microbiology

authors： Lee H,Park HJ,Cho SN,Bai GH,Kim SJ

更新日期：2000-08-01 00:00:00

abstract：:Restriction fragment length polymorphism and hybridization of DNA extracted from Mycobacterium tuberculosis, nontuberculous mycobacteria, and nonmycobacterial species with a probe derived from IS6110 confirmed that IS6110 was specific to M. tuberculosis complex. In addition, DNA amplification with IS6110-specific prim...

journal_title：Journal of clinical microbiology

authors： Githui WA,Wilson SM,Drobniewski FA

更新日期：1999-04-01 00:00:00

abstract：:A novel reverse primer (GLM MR1) was designed for detection of the glmM gene in Helicobacter pylori by PCR. The percentage of amplification in clinical isolates using GLM MR1 was 100% for detection of the glmM gene and 86.36% for the ureA gene. The primer designed is useful for the identification of H. pylori. ...

journal_title：Journal of clinical microbiology

authors： Espinoza MG,Vazquez RG,Mendez IM,Vargas CR,Cerezo SG

更新日期：2011-04-01 00:00:00

abstract：:The performance of a commercially available, rapid membrane enzyme immunoassay for influenza A and B virus detection was compared to that of viral culture in 4,092 respiratory specimens collected from patients presenting with respiratory symptoms during the 2002-2003 influenza season. The test's overall sensitivity wa...

journal_title：Journal of clinical microbiology

authors： Cazacu AC,Chung SE,Greer J,Demmler GJ

更新日期：2004-08-01 00:00:00

abstract：:A disease similar to ulcerative colitis in humans has been identified in cotton-top tamarins (CTTs) in captivity. The clinical signs include weight loss, diarrhea, and rectal bleeding with the pathological features and biochemical abnormalities of ulcerative colitis. Approximately 25 to 40% of these animals develop co...

journal_title：Journal of clinical microbiology

authors： Saunders KE,Shen Z,Dewhirst FE,Paster BJ,Dangler CA,Fox JG

更新日期：1999-01-01 00:00:00

abstract：:Community-acquired bloodstream infections cause substantial morbidity and mortality worldwide, but microbiology capacity and surveillance limitations have challenged good descriptions of pathogen distribution in many regions, including Southeast Asia. Active surveillance for bloodstream infections has been conducted i...

journal_title：Journal of clinical microbiology

authors： Jorakate P,Higdon M,Kaewpan A,Makprasert S,Yuenprakhon S,Tawisaid K,Dejsirilert S,Whistler T,Baggett HC

更新日期：2015-03-01 00:00:00

abstract：:Panton-Valentine leukocidin (PVL) is associated with staphylococcal skin and pulmonary infections. Community dissemination of PVL-producing Staphylococcus aureus strains constitutes a public health concern. Family transmission and spread of community-acquired leukocidin-positive methicillin-susceptible S. aureus ST152...

journal_title：Journal of clinical microbiology

authors： Pérez-Roth E,Alcoba-Flórez J,López-Aguilar C,Gutiérrez-González I,Rivero-Pérez B,Méndez-Alvarez S

更新日期：2010-01-01 00:00:00

abstract：:Nocardia takedensis is a recently described species isolated from soil. The first clinical isolate in Japan has recently been reported. This report describes the first clinical isolate of N. takedensis in Spain from a respiratory specimen. ...

journal_title：Journal of clinical microbiology

authors： Betrán A,Rezusta A,Lezcano MA,Villuendas MC,Revillo MJ,Boiron P,Rodríguez-Nava V

更新日期：2009-06-01 00:00:00

abstract：:Nosocomial oxacillin-resistant Staphylococcus aureus (ORSA) bloodstream isolates were tested to determine the prevalence of vancomycin heteroresistance. We screened 619 ORSA nosocomial bloodstream isolates from 36 hospitals between 1997 and 2000. Only one isolate exhibiting heterotypic resistance was detected. Thus, v...

journal_title：Journal of clinical microbiology

authors： Tallent SM,Bischoff T,Climo M,Ostrowsky B,Wenzel RP,Edmond MB

更新日期：2002-06-01 00:00:00

abstract：:In an initial experiment, culture-grown Borrelia burgdorferi was added to freshly collected uninfected human blood. This in vitro study demonstrated that more spirochetes were distributed into the plasma than into the serum fraction. In a subsequent clinical study, B. burgdorferi was recovered from plasma cultures of ...

journal_title：Journal of clinical microbiology

authors： Wormser GP,Bittker S,Cooper D,Nowakowski J,Nadelman RB,Pavia C

更新日期：2000-04-01 00:00:00

abstract：:Adolescent children were surveyed for colonization with Mycoplasma hominis and Ureaplasma urealyticum by culturing urine specimens. Rates were compared between three study groups: (i) 397 children attending parochial schools, (ii) 293 children attending an adolescent clinic specializing in adjustment problems, and (ii...

journal_title：Journal of clinical microbiology

authors： Foy H,Kenny G,Bor E,Hammar S,Hickman R

更新日期：1975-09-01 00:00:00

abstract：:Conventional culture techniques are limited in the ability to detect multiple Streptococcus pneumoniae serotypes in nasopharyngeal (NP) secretions. We developed an immunoblot (IB) method with monoclonal antibodies (MAbs) to detect S. pneumoniae and to identify serotypes. NP specimens stored in skim milk-tryptone-gluco...

journal_title：Journal of clinical microbiology

authors： Bronsdon MA,O'Brien KL,Facklam RR,Whitney CG,Schwartz B,Carlone GM

更新日期：2004-04-01 00:00:00

abstract：:Real-time PCR methodology can be applied to rapidly and accurately detect influenza viruses. During times of surge testing or enhanced pandemic surveillance, public health laboratories (PHLs) may experience overwhelming demand for testing, even while the prevalence of positive specimens remains low. To improve laborat...

journal_title：Journal of clinical microbiology

authors： Van TT,Miller J,Warshauer DM,Reisdorf E,Jernigan D,Humes R,Shult PA

更新日期：2012-03-01 00:00:00

abstract：:On occasion, a patient may have two or more clinical cultures yielding a coagulase-negative staphylococcus If these multiple isolates have the same phenotype, one might conclude that the same strain was reisolated from the patient, indicating its persistent and pathological presence. We examined the validity of this c...

journal_title：Journal of clinical microbiology

authors： Christensen GD,Parisi JT,Bisno AL,Simpson WA,Beachey EH

更新日期：1983-08-01 00:00:00

abstract：:We compared trends of annual resistance rates calculated from results for all isolates and for the first isolate of Staphylococcus aureus, Klebsiella pneumoniae, and Acinetobacter baumannii per patient over a 3-year period from 2001 through 2003. Antimicrobial susceptibility results of inpatients were extracted from a...

journal_title：Journal of clinical microbiology

authors： Lee SO,Cho YK,Kim SY,Lee ES,Park SY,Seo YH

更新日期：2004-10-01 00:00:00

abstract：:A Salmonella enterica serotype Typhi strain was cultured from blood and fecal samples from a 54-year-old man with fever and diarrhea. He had returned from travel to the Philippines a few days earlier. Phenotypic and genotypic analysis confirmed the production of the SHV-12 extended-spectrum beta-lactamase. ...

journal_title：Journal of clinical microbiology

authors： Al Naiemi N,Zwart B,Rijnsburger MC,Roosendaal R,Debets-Ossenkopp YJ,Mulder JA,Fijen CA,Maten W,Vandenbroucke-Grauls CM,Savelkoul PH

更新日期：2008-08-01 00:00:00

abstract：:Recent studies have shown the public health importance of identifying individuals with acute human immunodeficiency virus infection (AHI); however, the cost of nucleic acid amplification testing (NAAT) makes individual testing of at-risk individuals prohibitively expensive in many settings. Pooled NAAT (or group testi...

journal_title：Journal of clinical microbiology

authors： Westreich DJ,Hudgens MG,Fiscus SA,Pilcher CD

更新日期：2008-05-01 00:00:00