Molecular subtyping and characterization of bovine and human Streptococcus agalactiae isolates.

abstract：:Fully standardized reproducible and sensitive quantification assays for cytomegalovirus (CMV) are needed to better define thresholds for antiviral therapy initiation and interruption. We evaluated the newly released Abbott RealTime CMV assay for CMV quantification in whole blood (WB) that includes automated extraction...

journal_title：Journal of clinical microbiology

authors： Schnepf N,Scieux C,Resche-Riggon M,Feghoul L,Xhaard A,Gallien S,Molina JM,Socié G,Viglietti D,Simon F,Mazeron MC,Legoff J

更新日期：2013-07-01 00:00:00

abstract：:Rapid detection of drug resistance in Mycobacterium tuberculosis is essential for efficient treatment and control of this pathogen. The amplification refractory mutation system (ARMS) was used to detect mutations in the rifampin resistance-determining region of the rpoB gene. A total of 39 rifampin-resistant M. tuberc...

journal_title：Journal of clinical microbiology

authors： Fan XY,Hu ZY,Xu FH,Yan ZQ,Guo SQ,Li ZM

更新日期：2003-03-01 00:00:00

abstract：:In a large prospective comparison, the illumigene test detected Clostridium difficile in 98% of toxin-positive and 58% of toxin-negative samples confirmed positive by other methods. The Xpert was uniformly sensitive. Most samples with discrepant results had C. difficile concentrations below the illumigene limit of det...

journal_title：Journal of clinical microbiology

authors： Gyorke CE,Wang S,Leslie JL,Cohen SH,Solnick JV,Polage CR

更新日期：2013-01-01 00:00:00

abstract：:The genetic relatedness of Streptococcus milleri group isolates from the airways of cystic fibrosis patients was determined by using pulsed-field gel electrophoresis. This study reveals no evidence for patient-to-patient transmission in our patient population; however, within individual patients, complex inter- and in...

journal_title：Journal of clinical microbiology

authors： Sibley CD,Sibley KA,Leong TA,Grinwis ME,Parkins MD,Rabin HR,Surette MG

更新日期：2010-07-01 00:00:00

abstract：:Identification of clinical isolates of Nocardia to the species level is important for defining the spectrum of disease produced by each species and for predicting antimicrobial susceptibility. We evaluated the usefulness of PCR amplification of a portion of the Nocardia 16S rRNA gene and subsequent restriction endonuc...

journal_title：Journal of clinical microbiology

authors： Conville PS,Fischer SH,Cartwright CP,Witebsky FG

更新日期：2000-01-01 00:00:00

abstract：:The enzyme-linked immunosorbent assay (ELISA) has been proposed as an alternative serologic diagnostic test to the indirect immunofluorescence assay (IFA) for scrub typhus. Here, we systematically determine the optimal sample dilution and cutoff optical density (OD) and estimate the accuracy of IgM ELISA using Bayesia...

journal_title：Journal of clinical microbiology

authors： Blacksell SD,Lim C,Tanganuchitcharnchai A,Jintaworn S,Kantipong P,Richards AL,Paris DH,Limmathurotsakul D,Day NPJ

更新日期：2016-06-01 00:00:00

abstract：:The performance of the Etest for voriconazole and for itraconazole susceptibility testing of 376 isolates of Aspergillus spp. was assessed in comparison with the National Committee for Clinical Laboratory Standards (NCCLS) proposed standard microdilution broth method. The NCCLS method employed RPMI 1640 broth medium, ...

journal_title：Journal of clinical microbiology

authors： Pfaller JB,Messer SA,Hollis RJ,Diekema DJ,Pfaller MA

更新日期：2003-03-01 00:00:00

abstract：:Given that the integration of human papillomavirus type 16 (HPV16) into the host genome occurs preferentially with the disruption of the E2 gene, a ratio of E2 to E7 gene copies is often used as a marker for integration. It is largely undetermined, however, whether ratio estimates are affected by HPV intratypic variat...

journal_title：Journal of clinical microbiology

authors： Jiang M,Baseman JG,Koutsky LA,Feng Q,Mao C,Kiviat NB,Xi LF

更新日期：2009-03-01 00:00:00

abstract：:A heminested inverse PCR (HIP) for the amplification of sequences flanking the Mycobacterium tuberculosis insertion sequence IS6110 has been developed. The method depends upon primers that anneal to IS6110 at sites between its 5' end and the closest BsrFI site. The accuracy of HIP was demonstrated by the amplification...

journal_title：Journal of clinical microbiology

authors： Patel S,Wall S,Saunders NA

更新日期：1996-07-01 00:00:00

abstract：:Recent studies have speculated on the possible role of the mother in transmitting Helicobacter pylori infection to their children. In an attempt to either prove or disprove this supposition, we investigated the rates of infection of children born to H. pylori-positive mothers from birth to 5 years of age using serolog...

journal_title：Journal of clinical microbiology

authors： Konno M,Fujii N,Yokota S,Sato K,Takahashi M,Sato K,Mino E,Sugiyama T

更新日期：2005-05-01 00:00:00

abstract：:A new oligochromatographic assay, Speed-Oligo Novel Influenza A H1N1, was designed and optimized for the specific detection of the 2009 influenza A H1N1 virus. The assay is based on a PCR method coupled to detection of PCR products by means of a dipstick device. The target sequence is a 103-bp fragment within the hema...

journal_title：Journal of clinical microbiology

authors： Pérez-Ruiz M,Navarro-Marí JM,Bautista-Marín MF,Pedrosa-Corral I,Sanbonmatsu-Gámez S,Camacho AG,Rojas J,Ruiz-Ortiz J,Rodríguez-Granger J,Carrillo JA

更新日期：2010-05-01 00:00:00

abstract：:A hemolysin produced by Aeromonas hydrophila CA-11, isolated from an environmental source, was purified by sulfopropyl-Sephadex C-25 chromatography at pH 5.0. This hemolysin caused fluid accumulation in infant mouse intestines and rabbit intestinal loops and killed Vero cells, as did the hemolysin produced by strain A...

journal_title：Journal of clinical microbiology

authors： Asao T,Kozaki S,Kato K,Kinoshita Y,Otsu K,Uemura T,Sakaguchi G

更新日期：1986-08-01 00:00:00

abstract：:Evaluation of optimal compositions of blood culture media has called for extensive and laborious work in comparative studies of large series of clincal specimens. Bacterial growth is accompanied by heat production, and calorimetry provides an analytical tool for its detection and quantification. A twin microcalorimete...

journal_title：Journal of clinical microbiology

authors： Ripa KT,Mårdh PA,Hovelius B,Ljungholm K

更新日期：1977-04-01 00:00:00

abstract：:Bacillus anthracis spores have been used as a biological weapon in the United States. We wanted to develop a safe, rapid method of sample preparation that provided safe DNA for the detection of spores in environmental and clinical specimens. Our method reproducibly detects B. anthracis in samples containing <10 spores...

journal_title：Journal of clinical microbiology

authors： Luna VA,King D,Davis C,Rycerz T,Ewert M,Cannons A,Amuso P,Cattani J

更新日期：2003-03-01 00:00:00

abstract：:Helicobacter pylori cagA and vacA genotypes have been used for almost a decade as stable entities to link the severity of gastritis and ulcer disease. We describe here microevolution of the two genomic islands, cag pathogenicity island (cagPAI; 40 kb) and tfs3 (16 kb) from isolates obtained at inclusion (one subclone)...

journal_title：Journal of clinical microbiology

authors： Alvi A,Devi SM,Ahmed I,Hussain MA,Rizwan M,Lamouliatte H,Mégraud F,Ahmed N

更新日期：2007-12-01 00:00:00

abstract：:Rabies virus found worldwide and prevalent throughout the United States continues to be a public health concern. Direct-fluorescent antibody (DFA) detection remains the gold standard for rabies virus diagnostics. Assessing the utility of a high-throughput molecular platform such as the QIAsymphony SP/AS, in conjunctio...

journal_title：Journal of clinical microbiology

authors： Dupuis M,Brunt S,Appler K,Davis A,Rudd R

更新日期：2015-09-01 00:00:00

abstract：:Formalin-fixed, purified reticulate bodies (RB) of Chlamydia trachomatis immunotype C/TW-3/OT were used as a serological test antigen in the microimmunofluorescence test. The sensitivity and specificity of the RB antigen were compared to elementary bodies (EB) used as antigens in the detection of C. trachomatis antibo...

journal_title：Journal of clinical microbiology

authors： Yong EC,Chinn JS,Caldwell HD,Kuo CC

更新日期：1979-09-01 00:00:00

abstract：:Clinical isolates of Haemophilus influenzae were assessed for the presence of fucK, hap, and sodC by hybridization with gene-specific probes, and isolates diverging from the expected H. influenzae genotype were characterized by phenotype and 16S rRNA gene sequencing. Two of 480 isolates were finally classified as vari...

journal_title：Journal of clinical microbiology

authors： Nørskov-Lauritsen N

更新日期：2009-08-01 00:00:00

abstract：:Cag pathogenicity island-containing Helicobacter pylori (type I) induces signal transduction pathways resulting in tyrosine phosphorylation of proteins adjacent to the site of bacterial adhesion on host gastric epithelial cells. Conventional block PCR-restriction fragment length polymorphism (RFLP) and real-time Light...

journal_title：Journal of clinical microbiology

authors： Owen RJ,Sharp SI,Chisholm SA,Rijpkema S

更新日期：2003-07-01 00:00:00

abstract：:Studies were conducted to compare and evaluate antimicrobial susceptibility test results for enterococci obtained by six national committee disk diffusion procedures. Variations in the incidence of isolates in resistance categories and errors were associated with the use of ciprofloxacin, gentamicin, nitrofurantoin, r...

journal_title：Journal of clinical microbiology

authors： Cotter G,Adley CC

更新日期：2001-10-01 00:00:00

abstract：:Seventy-six nontuberculous mycobacterial isolates obtained from patients living in Greece were analyzed with the GenoType Mycobacterium CM (for common mycobacteria) and AS (for additional species) assays. GenoType correctly identified all but one of the mycobacterial species. For this species, additional probes should...

journal_title：Journal of clinical microbiology

authors： Gitti Z,Neonakis I,Fanti G,Kontos F,Maraki S,Tselentis Y

更新日期：2006-06-01 00:00:00

abstract：:The in vitro susceptibilities of 66 molecularly identified strains of the Mucorales to eight antifungals (amphotericin B, terbinafine, itraconazole, posaconazole, voriconazole, caspofungin, micafungin, and 5-fluorocytosine) were tested. Molecular phylogeny was reconstructed based on the nuclear ribosomal large subunit...

journal_title：Journal of clinical microbiology

authors： Vitale RG,de Hoog GS,Schwarz P,Dannaoui E,Deng S,Machouart M,Voigt K,van de Sande WW,Dolatabadi S,Meis JF,Walther G

更新日期：2012-01-01 00:00:00

abstract：:CHROMagar Candida is a selective and differential chromogenic medium that has been shown to be useful for identification of Candida albicans, Candida krusei, Candida tropicalis, and perhaps Candida glabrata. Colony morphology and color have been well defined when CHROMagar Candida has been used to isolate yeast direct...

journal_title：Journal of clinical microbiology

authors： Horvath LL,Hospenthal DR,Murray CK,Dooley DP

更新日期：2003-06-01 00:00:00

abstract：:Invasive collection methods are often required to obtain samples for the microbiological evaluation of children with presumptive pulmonary tuberculosis (PTB). Nucleic acid amplification testing of easier-to-collect stool samples could be a noninvasive method of diagnosing PTB. We conducted a systematic review and meta...

journal_title：Journal of clinical microbiology

authors： MacLean E,Sulis G,Denkinger CM,Johnston JC,Pai M,Ahmad Khan F

更新日期：2019-05-24 00:00:00

abstract：:The ERA strain of rabies virus was adapted to growth on monolayers of a porcine kidney cell line (PK-2A). A reproducible plaque assay system was subsequently developed, which appears to be satisfactory for conducting plaque neutralization tests. ...

journal_title：Journal of clinical microbiology

authors： Strating A,Mitzel JR,Slater EA

更新日期：1976-08-01 00:00:00

abstract：:We studied the evolution of Vibrio cholerae O1 during the 1991 to 2000 cholera epidemic in Mexico by biochemical, serological, and molecular characterization of strains collected during this period. Strains were divided into toxigenic and nontoxigenic groups according to the presence or absence of genes encoding chole...

journal_title：Journal of clinical microbiology

authors： Lizárraga-Partida ML,Quilici ML

更新日期：2009-05-01 00:00:00

abstract：:Central nervous system cysticercosis is common in countries where Taenia solium occurs in pigs and the level of hygiene and sanitation is low. The clinical presentation may include epileptic seizures, focal neurological deficits, hydrocephalus, or aseptic meningitis. The disease is frequently seen in California reside...

journal_title：Journal of clinical microbiology

authors： Mohammad IN,Heiner DC,Miller BL,Goldberg MA,Kagan IG

更新日期：1984-10-01 00:00:00

abstract：:The performance of a human herpesvirus 8 (HHV-8) enzyme immunoassay (EIA) and selective subsequent use of an HHV-8 immunofluorescence assay (IFA) was tested in persons unlikely to be infected with HHV-8 and those who had HHV-8 detected in their saliva. The IFA was performed on samples within a range of EIA optical den...

journal_title：Journal of clinical microbiology

authors： Casper C,Krantz E,Taylor H,Dalessio J,Carrell D,Wald A,Corey L,Ashley R

更新日期：2002-10-01 00:00:00

abstract：:A total of 238 isolates of Providencia stuartii obtained from infected patients in six Dublin hospitals were grouped by using serological and bacteriocin typing methods and tested for sensitivity to a number of antimicrobial agents. Most isolates were resistant to several of these agents. Resistance to tetracycline, r...

journal_title：Journal of clinical microbiology

authors： McHale PJ,Keane CT,Dougan G

更新日期：1981-06-01 00:00:00

abstract：:Lipopolysaccharide (LPS) of a reference strain of Yersinia enterocolitica serovar O15, Ye614, was examined for its chemical structure and antigenicity. The O15 LPS was partially hydrolyzed with diluted acetic acid and fractionated on a Sephadex G-50 column. Only one peak was detected at fractions where the R-core olig...

journal_title：Journal of clinical microbiology

authors： Kawaoka Y,Wauters G,Otsuki K,Tsubokura M

更新日期：1986-08-01 00:00:00