Purification and characterization of an Aeromonas hydrophila hemolysin.

abstract：:Conditions were defined for functional covalent coupling of anti-Lassa virus globulins to glutaraldehyde-fixed chicken erythrocytes. Tolylene-2,4-diisocyanate in a reaction mixture containing not more than 0.01 M NaCl produced uniformly good conjugates which were used in reversed passive hemagglutination (RPH) and rev...

journal_title：Journal of clinical microbiology

authors： Goldwasser RA,Elliott LH,Johnson KM

更新日期：1980-06-01 00:00:00

abstract：:Cowan I staphylococci coated with antisera to streptococcal groups A, B, C, D, F, and G were used as coagglutination reagents in a modified coagglutination procedure (MCAP). Streptococcal group antigens were extracted with a Streptomyces albus-lysozyme enzyme mixture for 30 min at 55 degrees C and centrifuged, and the...

journal_title：Journal of clinical microbiology

authors： Carlson JR,McCarthy LR

更新日期：1979-03-01 00:00:00

abstract：:Bilophila wadsworthia is a common inhabitant of the human colon and has been associated with appendicitis and other local sites of inflammation in humans. Challenge-exposure or prevalence studies in laboratory and other animals have not been reported. B. wadsworthia is closely related phylogenetically to Desulfovibrio...

journal_title：Journal of clinical microbiology

authors： McOrist AL,Warhurst M,McOrist S,Bird AR

更新日期：2001-04-01 00:00:00

abstract：:In our nursery, we identified neonates with positive urine latex particle agglutination (LPA) tests for group B streptococcus (GBS) antigen who did not have corroborating cultural evidence of infection. To investigate the mechanisms underlying these apparent false-positive reactions, we examined the urine LPA test in ...

journal_title：Journal of clinical microbiology

authors： Harris MC,Deuber C,Polin RA,Nachamkin I

更新日期：1989-10-01 00:00:00

abstract：:An accurate, rapid, and inexpensive method was developed for detecting and enumerating bacteria adherent to Foley urinary catheters based on malachite green staining of acridine orange-prestained specimens. This method has proven to be quick and reliable and will find application in quantitative studies of biomaterial...

journal_title：Journal of clinical microbiology

authors： Ladd TI,Schmiel D,Nickel JC,Costerton JW

更新日期：1985-06-01 00:00:00

abstract：:From late 2003 to the end of 2005, the Health Protection Agency's national reference laboratories received approximately 1,600 referrals of Acinetobacter spp., including 419 and 58 examples, respectively, of two carbapenem-resistant Acinetobacter baumannii lineages, designated OXA-23 clones 1 and 2. Representatives of...

journal_title：Journal of clinical microbiology

authors： Coelho JM,Turton JF,Kaufmann ME,Glover J,Woodford N,Warner M,Palepou MF,Pike R,Pitt TL,Patel BC,Livermore DM

更新日期：2006-10-01 00:00:00

abstract：:Twenty-four isolates of Actinobacillus seminis were typed by PCR ribotyping, repetitive extragenic palindromic element (REP)-based PCR, and enterobacterial repetitive intergenic consensus (ERIC)-based PCR. Five types were distinguished by REP-PCR, and nine types were distinguished by ERIC-PCR. PCR ribotyping produced ...

journal_title：Journal of clinical microbiology

authors： Appuhamy S,Coote JG,Low JC,Parton R

更新日期：1998-03-01 00:00:00

abstract：:In this issue of the Journal of Clinical Microbiology, R. E. Bumgarner, D. Harrison, and J. E. Hsu (J Clin Microbiol 58:e00121-19, 2020, https://doi.org/10.1128/JCM.00121-19) address in a retrospective analysis that clonality of Cutibacterium acnes isolates from deep tissue specimens obtained from patients during revi...

journal_title：Journal of clinical microbiology

authors： Schuetz AN

更新日期：2020-01-28 00:00:00

abstract：:The Gen-Probe Amplified Mycobacterium tuberculosis direct test is widely used in the diagnosis of tuberculosis. Specimens may contain amplification inhibitors, potentially leading to false-negative results if unrecognized. We report a failure to detect inhibition, despite adherence to the inhibition testing guidelines...

journal_title：Journal of clinical microbiology

authors： Kim JY,Ferraro MJ,Branda JA

更新日期：2009-09-01 00:00:00

abstract：:Aeromonas caviae was recovered as the sole potential enteric pathogen from the stools of 14 of 17 symptomatic children (10 younger than 1 year of age) while Aeromonas hydrophila, Aeromonas sobria, and Plesiomonas shigelloides were isolated once each. The infants from whom A. caviae was isolated all presented with a wa...

journal_title：Journal of clinical microbiology

authors： Namdari H,Bottone EJ

更新日期：1990-05-01 00:00:00

abstract：:A new rapid one-step immunochromatographic test using monoclonal antibodies for detection of Helicobacter pylori antigen in stool in children was evaluated on coded stool samples from 159 children (mean age, 9.7 +/- 5.0 years; 118 from Munich, 41 from Vienna): 86 children were H. pylori infected defined by positive cu...

journal_title：Journal of clinical microbiology

authors： Antos D,Crone J,Konstantopoulos N,Koletzko S

更新日期：2005-06-01 00:00:00

abstract：:Classical microbiological diagnosis of human brucellosis is time-consuming, hazardous, and subject to variable interpretation. Intact-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was evaluated for the routine identification of Brucella spp. Analysis of mass peak patt...

journal_title：Journal of clinical microbiology

authors： Karger A,Melzer F,Timke M,Bettin B,Kostrzewa M,Nöckler K,Hohmann A,Tomaso H,Neubauer H,Al Dahouk S

更新日期：2013-09-01 00:00:00

abstract：:We have developed a high-resolution melting (HRM) assay to scan for mutations in the rpoB, inhA, ahpC, and katG genes and/or promoter regions for the detection of rifampin and isoniazid resistance in Mycobacterium tuberculosis. For assay development, 23 drug-resistant isolates of M. tuberculosis having 29 different mu...

journal_title：Journal of clinical microbiology

authors： Ong DC,Yam WC,Siu GK,Lee AS

更新日期：2010-04-01 00:00:00

abstract：:This study used a nested multiplex PCR method to detect three periodontal pathogens in subgingival plaque collected before treatment and at 2 and 6 months posttreatment from 107 patients with severe, generalized periodontitis. The proportions of the patients who harbored these bacteria before periodontal treatment wer...

journal_title：Journal of clinical microbiology

authors： Ready D,D'Aiuto F,Spratt DA,Suvan J,Tonetti MS,Wilson M

更新日期：2008-10-01 00:00:00

abstract：:A total of 100 random clinical urine specimens were divided and used to compare Becton, Dickinson & Co. B-D Urine Culture Kit-treated urine (BDU) with refrigerated urine (RU). Each RU-BDU pair was cultured with the standard calibrated loop-plate method and also screened with the Abbott Laboratories MS-2 system at 0, 5...

journal_title：Journal of clinical microbiology

authors： Hubbard WA,Shalis PJ,McClatchey KD

更新日期：1983-02-01 00:00:00

abstract：:Quantitative lactate determinations were performed on cerebrospinal fluids to assess their value in the rapid diagnosis of bacterial and mycotic meningitis and to evaluate their value in assessing the prognosis in these patients. Cerebrospinal fluid lactate concentrations were elevated in all patients with untreated b...

journal_title：Journal of clinical microbiology

authors： Komorowski RA,Farmer SG,Hanson GA,Hause LL

更新日期：1978-07-01 00:00:00

abstract：:The radioimmunoprecipitation assay (RIPA) has been used as a confirmatory test in several ongoing and published studies of Trypanosoma cruzi in blood donors in the United States. Despite its use as a confirmatory test, few studies are available comparing RIPA to commercially available serologic test methods. Thus, we ...

journal_title：Journal of clinical microbiology

authors： Leiby DA,Wendel S,Takaoka DT,Fachini RM,Oliveira LC,Tibbals MA

更新日期：2000-02-01 00:00:00

abstract：:Totals of 102 and 56 Clostridium difficile type 078 strains of human and porcine origins, respectively, from four European countries were investigated by an optimized multilocus variable-number tandem-repeat analysis (MLVA) and for tetracycline susceptibility. Eighty-five percent of all isolates were genetically relat...

journal_title：Journal of clinical microbiology

authors： Bakker D,Corver J,Harmanus C,Goorhuis A,Keessen EC,Fawley WN,Wilcox MH,Kuijper EJ

更新日期：2010-10-01 00:00:00

abstract：:Quantitative fecal lactoferrin was measured in 112 patients tested for toxigenic Clostridium difficile using glutamate dehydrogenase (GDH) and toxin immunoassays combined with tcdB PCR. Lactoferrin levels were higher in the GDH-positive/toxin-positive group (79 μg/ml) than in the GDH-positive/toxin-negative/PCR-positi...

journal_title：Journal of clinical microbiology

authors： LaSala PR,Ekhmimi T,Hill AK,Farooqi I,Perrotta PL

更新日期：2013-01-01 00:00:00

abstract：:RotaTeq (RV5) is a widely used live attenuated pentavalent rotavirus (RV) vaccine. Although fecal shedding of RV vaccine strains persists for long time periods, it is unclear how each vaccine strain replicates in intestinal tissue and is excreted in stool. To examine this issue, we established RV5 genotype-specific re...

journal_title：Journal of clinical microbiology

authors： Higashimoto Y,Ihira M,Miyazaki Y,Kuboshiki A,Yoshinaga S,Hiramatsu H,Suzuki R,Miyata M,Miura H,Komoto S,Yukitake J,Taniguchi K,Kawamura Y,Yoshikawa T

更新日期：2018-05-25 00:00:00

abstract：:Timely diagnosis of respiratory syncytial virus (RSV) infection is critical for appropriate treatment of lower respiratory infection in young children. To facilitate diagnosis, we developed a rapid, specific, and sensitive TaqMan PCR method for detection of RSV A and RSV B. Two sets of primer-probe pairs were selected...

journal_title：Journal of clinical microbiology

authors： Hu A,Colella M,Tam JS,Rappaport R,Cheng SM

更新日期：2003-01-01 00:00:00

abstract：:Several unusual fatty acids characterized strains of Francisella tularensis. Long-chain (C20-C26) acids and the hydroxy acids 2-hydroxy-decanoate, 3-hydroxy-hexadecanoate, and 3-hydroxy-octadecanoate appeared to be of special diagnostic value. ...

journal_title：Journal of clinical microbiology

authors： Jantzen E,Berdal BP,Omland T

更新日期：1979-12-01 00:00:00

abstract：:To detect Streptococcus pneumoniae colonization, the nasopharynx is sampled using a swab placed in skim milk-tryptone-glucose-glycerol (STGG) transport medium, and then the swab specimen or STGG medium is cultured or subjected to PCR. We evaluated the effect of swab composition and compared the sensitivities of detect...

journal_title：Journal of clinical microbiology

authors： Rubin LG,Rizvi A,Baer A

更新日期：2008-08-01 00:00:00

abstract：:The QuadFERM+ system (Analytab Products, Plainview, N.Y.), a 2-h carbohydrate degradation method for the identification of Neisseria spp., was evaluated along with a rapid DNase test for confirmation of Branhamella catarrhalis. QuadFERM+ identified 100% of 82 N. gonorrhoeae and 96% of 54 N. meningitidis strains. The t...

journal_title：Journal of clinical microbiology

authors： Janda WM,Zigler KL,Bradna JJ

更新日期：1987-02-01 00:00:00

abstract：:Human herpesvirus 6 (HHV-6) variant B is frequently identified in peripheral blood, but identification of HHV-6 variant A is relatively rare. We devised a PCR-based method for sensitive, simultaneous detection of both HHV-6 variants. The method was applied to 34 lung tissue specimens that were previously shown to cont...

journal_title：Journal of clinical microbiology

authors： Cone RW,Huang ML,Hackman RC,Corey L

更新日期：1996-04-01 00:00:00

abstract：:Diagnostic tests for respiratory viral infections have traditionally been performed on nasopharyngeal swabs or washings. Reverse transcriptase PCR (RT-PCR) is rapid, sensitive, and specific for viral infection diagnosis but is rarely applied to sputum samples. Thus, we evaluated the diagnostic yield of RT-PCR for dete...

journal_title：Journal of clinical microbiology

authors： Falsey AR,Formica MA,Walsh EE

更新日期：2012-01-01 00:00:00

abstract：:A combination of immunomagnetic separation (IMS) and a polymerase chain reaction (PCR) procedure was used for direct isolation and identification of Shigella dysenteriae type 1 and Shigella flexneri from feces. Immunomagnetic particles were coated with monoclonal antibody MASFB, which is specific for a common epitope ...

journal_title：Journal of clinical microbiology

authors： Islam D,Lindberg AA

更新日期：1992-11-01 00:00:00

abstract：:CDC group M-5 is a rod-shaped, gram-negative, nonmotile bacterium associated with dog bite wounds. DNA-DNA relatedness and biochemical and growth characteristics were studied for 54 strains from the collection at the Centers for Disease Control and Prevention. One typical M-5 strain, 8142, was further studied by 16S r...

journal_title：Journal of clinical microbiology

authors： Andersen BM,Steigerwalt AG,O'Connor SP,Hollis DG,Weyant RS,Weaver RE,Brenner DJ

更新日期：1993-09-01 00:00:00

abstract：:A procedure was developed for characterization of Chlamydia trachomatis strains by using restriction endonuclease analysis of amplified genes of the major outer membrane protein (MOMP). Reference strains of the 15 serovars (A through K and L1 through L3) and clinical isolates were tested. The nucleotide sequences of t...

journal_title：Journal of clinical microbiology

authors： Rodriguez P,Vekris A,de Barbeyrac B,Dutilh B,Bonnet J,Bebear C

更新日期：1991-06-01 00:00:00

abstract：:A dot immunobinding assay was used to detect antibodies to measles virus, mumps virus, and rubella virus antigens. Filter paper soaked with serum or whole blood was directly applied to the antigen-coated nitrocellulose sheets. The test was easy to perform, and its results agreed very well with those obtained by standa...

journal_title：Journal of clinical microbiology

authors： Condorelli F,Ziegler T

更新日期：1993-03-01 00:00:00