Investigation of apparent false-positive urine latex particle agglutination tests for the detection of group B streptococcus antigen.

abstract：:The radioimmunoprecipitation assay (RIPA) has been used as a confirmatory test in several ongoing and published studies of Trypanosoma cruzi in blood donors in the United States. Despite its use as a confirmatory test, few studies are available comparing RIPA to commercially available serologic test methods. Thus, we ...

journal_title：Journal of clinical microbiology

authors： Leiby DA,Wendel S,Takaoka DT,Fachini RM,Oliveira LC,Tibbals MA

更新日期：2000-02-01 00:00:00

abstract：:Paracoccidioides brasiliensis isolates from 10 nine-banded armadillos (Dasypus novemcinctus) were comparable with 19 clinical isolates by sequence analysis of the PbGP43 gene and ribosomal internal transcribed spacer 1 (ITS1) and ITS2 and by random amplified polymorphic DNA. In this original ITS study, eight isolates ...

journal_title：Journal of clinical microbiology

authors： Hebeler-Barbosa F,Morais FV,Montenegro MR,Kuramae EE,Montes B,McEwen JG,Bagagli E,Puccia R

更新日期：2003-12-01 00:00:00

abstract：:More than 700 bacterial species or phylotypes, of which over 50% have not been cultivated, have been detected in the oral cavity. Our purposes were (i) to utilize culture-independent molecular techniques to extend our knowledge on the breadth of bacterial diversity in the healthy human oral cavity, including not-yet-c...

journal_title：Journal of clinical microbiology

authors： Aas JA,Paster BJ,Stokes LN,Olsen I,Dewhirst FE

更新日期：2005-11-01 00:00:00

abstract：:To determine the excretion dynamics and genotypic characteristics of rotavirus A (RVA), a longitudinal observational study was performed in 10 pigs from 3 litters at a farrow-to-finish farm. A total of 400 fecal samples were directly collected from the rectums of individual pigs (aged 7 to 217 days) at 3- to 14-day in...

journal_title：Journal of clinical microbiology

authors： Miyazaki A,Kuga K,Suzuki T,Tsunemitsu H

更新日期：2012-06-01 00:00:00

abstract：:In 1998, 21 inhabitants of a German nursing home fell ill with acute gastroenteritis after consumption of minced beef heart (P. Graf and L. Bader, Epidemiol. Bull. 41:327-329, 2000). Two residents died during hospital treatment. Seventeen Clostridium perfringens strains were collected from two different dishes and fro...

journal_title：Journal of clinical microbiology

authors： Schalch B,Bader L,Schau HP,Bergmann R,Rometsch A,Maydl G,Kessler S

更新日期：2003-02-01 00:00:00

abstract：:We report on a reverse line blot (RLB) assay, utilizing fungal species-specific oligonucleotide probes to hybridize with internal transcribed spacer 2 region sequences amplified using a nested panfungal PCR. Reference and clinical strains of 16 Candida species (116 strains), Cryptococcus neoformans (five strains of Cr...

journal_title：Journal of clinical microbiology

authors： Playford EG,Kong F,Sun Y,Wang H,Halliday C,Sorrell TC

更新日期：2006-03-01 00:00:00

abstract：:The detection of class A serine-carbapenemases among species of Enterobacteriaceae remains a challenging issue. Methods of identification for routine use in clinical microbiology laboratories have not been standardized to date. We developed a novel screening methodology suitable for countries with high basal levels of...

journal_title：Journal of clinical microbiology

authors： Pasteran F,Mendez T,Guerriero L,Rapoport M,Corso A

更新日期：2009-06-01 00:00:00

abstract：:Failure to demonstrate immunoglobulin M (IgM) antibodies by indirect immunofluorescence (IgM-IFA) in sera from some patients with acute acquired toxoplasmosis has recently been attributed to an inhibitory effect of high titers of IgG antibodies in these sera (Pyndiah et al. J. Clin. Microbiol. 9:170-174, 1979). To con...

journal_title：Journal of clinical microbiology

authors： Filice GA,Yeager AS,Remington JS

更新日期：1980-09-01 00:00:00

abstract：:Three cloned segments of Mycobacterium tuberculosis DNA which are promising as clinical probes were identified. An MboI digest of DNA from a clinical isolate of M. tuberculosis was cloned into bacteriophage M13. To identify recombinants specific for the M. tuberculosis complex, plaque lifts were hybridized with M. bov...

journal_title：Journal of clinical microbiology

authors： Eisenach KD,Crawford JT,Bates JH

更新日期：1988-11-01 00:00:00

abstract：:Two cyp51-related genes (cyp51A and cyp51B) encoding 14-alpha sterol demethylase-like enzymes were identified in the opportunistic human pathogen Aspergillus fumigatus. PCR amplification using degenerate oligonucleotides based on conserved areas of cytochrome P450 demethylases of other filamentous fungi and yeasts all...

journal_title：Journal of clinical microbiology

authors： Mellado E,Diaz-Guerra TM,Cuenca-Estrella M,Rodriguez-Tudela JL

更新日期：2001-07-01 00:00:00

abstract：:Rhodotorula species are emerging pathogens in immunocompromised patients. We report the in vitro activities of eight antifungals against 64 Rhodotorula isolates collected in surveillance programs between 1987 and 2003. Rhodotorula strains are resistant in vitro to fluconazole (MIC at which 50% of the isolates tested a...

journal_title：Journal of clinical microbiology

authors： Diekema DJ,Petroelje B,Messer SA,Hollis RJ,Pfaller MA

更新日期：2005-01-01 00:00:00

abstract：:Tests commonly used for routine determination of anti-Toxoplasma gondii immunoglobulin G (IgG) antibodies show a high level of consistency. However, considerable variations between commercial screening tests are still observed in detecting antibodies present at low concentrations, leading to a number of discrepant and...

journal_title：Journal of clinical microbiology

authors： Franck J,Garin YJ,Dumon H

更新日期：2008-07-01 00:00:00

abstract：:The AMPLICOR PCR was used to detect Neisseria gonorrhoeae in endocervical specimens. A 16S rRNA PCR performed on N. gonorrhoeae-positive samples showed sensitivities of 73.2, 64.3, and 94.4% for samples treated directly with AMPLICOR lysis buffer, samples suspended in 2-sucrose phosphate, and samples suspended in dilu...

journal_title：Journal of clinical microbiology

authors： Van Dyck E,Smet H,Van Damme L,Laga M

更新日期：2001-06-01 00:00:00

abstract：:Bordetella pertussis is a Gram-negative bacterium that causes respiratory infections in humans. Ongoing molecular surveillance of B. pertussis acellular vaccine (aP) antigens is critical for understanding the interaction between evolutionary pressures, disease pathogenesis, and vaccine effectiveness. Methods currently...

journal_title：Journal of clinical microbiology

authors： Marchand-Austin A,Tsang RSW,Guthrie JL,Ma JH,Lim GH,Crowcroft NS,Deeks SL,Farrell DJ,Jamieson FB

更新日期：2017-05-01 00:00:00

abstract：:During 5.5 years of a study in Washington, D.C., hospitalizations of children for rotavirus gastroenteritis tended to be more common after a month of cold or dry weather than after a corresponding calendar month of warm or wet weather. Overall, there were 84% more (178 versus 97) inpatients with rotavirus gastroenteri...

journal_title：Journal of clinical microbiology

authors： Brandt CD,Kim HW,Rodriguez WJ,Arrobio JO,Jeffries BC,Parrott RH

更新日期：1982-09-01 00:00:00

abstract：:Chlamydia pecorum is a significant pathogen of domestic livestock and wildlife. We have developed a C. pecorum-specific multilocus sequence analysis (MLSA) scheme to examine the genetic diversity of and relationships between Australian sheep, cattle, and koala isolates. An MLSA of seven concatenated housekeeping gene ...

journal_title：Journal of clinical microbiology

authors： Jelocnik M,Frentiu FD,Timms P,Polkinghorne A

更新日期：2013-08-01 00:00:00

abstract：:The global spread of carbapenemase-producing Enterobacteriaceae (CPE) that are often resistant to most, if not all, classes of antibiotics is a major public health concern. The NDM-1 carbapenemase is among the most worrisome carbapenemases given its rapid worldwide spread. We have developed and evaluated a lateral flo...

journal_title：Journal of clinical microbiology

authors： Boutal H,Naas T,Devilliers K,Oueslati S,Dortet L,Bernabeu S,Simon S,Volland H

更新日期：2017-07-01 00:00:00

abstract：:In this study, we evaluated a rapid whole-blood test, BM-test Helicobacter pylori, for detection of H. pylori infection in 144 and 48 patients with other gastrointestinal symptoms and with gastric cancer, respectively. The sensitivity, specificity, positive predictive value, negative predictive value, and diagnostic a...

journal_title：Journal of clinical microbiology

authors： Enroth H,Rigo R,Hultén K,Engstrand L

更新日期：1997-10-01 00:00:00

abstract：:An enzyme immunoassay (EIA) for serum immunoglobulin M (IgM) antibodies to rubella virus based on enzyme labeling of viral antigen was developed. The sensitivity of the EIA for the detection of recent rubella virus infection was evaluated by using 115 rubella-IgM-antibody-positive serum specimens, which were confirmed...

journal_title：Journal of clinical microbiology

authors： Seppänen H

更新日期：1990-04-01 00:00:00

abstract：:CDC group M-5 is a rod-shaped, gram-negative, nonmotile bacterium associated with dog bite wounds. DNA-DNA relatedness and biochemical and growth characteristics were studied for 54 strains from the collection at the Centers for Disease Control and Prevention. One typical M-5 strain, 8142, was further studied by 16S r...

journal_title：Journal of clinical microbiology

authors： Andersen BM,Steigerwalt AG,O'Connor SP,Hollis DG,Weyant RS,Weaver RE,Brenner DJ

更新日期：1993-09-01 00:00:00

abstract：:Noroviruses (NVs) are important human pathogens that cause acute gastroenteritis. Genetically related animal enteric NVs have also been described, but there is no evidence of interspecies transmission of NVs. In this study we characterized antibody prevalence among domestic pigs by using recombinant capsid antigens of...

journal_title：Journal of clinical microbiology

authors： Farkas T,Nakajima S,Sugieda M,Deng X,Zhong W,Jiang X

更新日期：2005-02-01 00:00:00

abstract：:Explanted orthopedic implants from 54 patients with aseptic failure and 24 patients with prosthetic knee or hip infection were sonicated in polyethylene bags. The sensitivities of periprosthetic tissue and sonicate fluid cultures for the diagnosis of prosthetic joint infection were 54% and 75%, whereas the specificiti...

journal_title：Journal of clinical microbiology

authors： Trampuz A,Piper KE,Hanssen AD,Osmon DR,Cockerill FR,Steckelberg JM,Patel R

更新日期：2006-02-01 00:00:00

abstract：:Over a 12-month period, 43 children in eight different wards of our hospital (Hôpital Robert Debré) were infected or colonized with Klebsiella pneumoniae strains producing extended broad-spectrum beta-lactamases. The epidemiology of the outbreak was studied by a molecular approach including the determination of the be...

journal_title：Journal of clinical microbiology

authors： Bingen EH,Desjardins P,Arlet G,Bourgeois F,Mariani-Kurkdjian P,Lambert-Zechovsky NY,Denamur E,Philippon A,Elion J

更新日期：1993-02-01 00:00:00

abstract：:A highly sensitive second-generation hepatitis C virus (HCV) core antigen assay has recently been developed. We compared viral disappearance and first-phase kinetics between commercially available core antigen (Ag) assays, Lumipulse Ortho HCV Ag (Lumipulse-Ag), and a quantitative HCV RNA PCR assay, Cobas Amplicor HCV ...

journal_title：Journal of clinical microbiology

authors： Takahashi M,Saito H,Higashimoto M,Atsukawa K,Ishii H

更新日期：2005-01-01 00:00:00

abstract：:The DNA fingerprint profiles and serotypes of 63 avian Pasteurella multocida field isolates, 13 attenuated vaccine isolates (propagated from vaccines manufactured by five companies), and 16 somatic reference strains were compared. DNA fingerprinting established the relationship of isolates that could not be distinguis...

journal_title：Journal of clinical microbiology

authors： Wilson MA,Morgan MJ,Barger GE

更新日期：1993-02-01 00:00:00

abstract：:We compared the coreceptor tropism-predicting performance of a specific genotypic algorithm for HIV-1 subtype D and that of the geno2pheno algorithm with different cutoffs. The D-specific algorithm and geno2pheno with a false-positivity rate cutoff of 2.5% had the same concordance with the phenotypic determination. Th...

journal_title：Journal of clinical microbiology

authors： Dina J,Raymond S,Maillard A,Le Guillou-Guillemette H,Rodalec A,Beby-Defaux A,Giraudeau G,Vallet S,Mourez T,Payan C,Vabret A,Ruffault A,Ferre V,Izopet J,Plantier JC

更新日期：2013-09-01 00:00:00

abstract：:In Korea, vancomycin-resistant enterococci have become important nosocomial pathogens since the late 1990s, and most vancomycin-resistant enterococcal isolates have been VanA phenotype-vanA genotype strains. In 2001, we experienced an outbreak of VanB phenotype-vanA genotype vancomycin-resistant enterococci at a unive...

journal_title：Journal of clinical microbiology

authors： Eom JS,Hwang IS,Hwang BY,Lee JG,Lee YJ,Cheong HJ,Park YH,Park SC,Kim WJ

更新日期：2004-04-01 00:00:00

abstract：:Cryptococcus gattii emerged in 1999 in a distinct ecologic environment on Vancouver Island, Canada. Estimates of incubation period were derived from an analysis of travelers with discreet exposures to this region. Individual case incubation periods ranged from 2 to 11 months, with a median of 6 to 7 months. ...

journal_title：Journal of clinical microbiology

authors： MacDougall L,Fyfe M

更新日期：2006-05-01 00:00:00

abstract：:Identification of clinical isolates of Nocardia to the species level is important for defining the spectrum of disease produced by each species and for predicting antimicrobial susceptibility. We evaluated the usefulness of PCR amplification of a portion of the Nocardia 16S rRNA gene and subsequent restriction endonuc...

journal_title：Journal of clinical microbiology

authors： Conville PS,Fischer SH,Cartwright CP,Witebsky FG

更新日期：2000-01-01 00:00:00

abstract：:A three-step method for the purification of Enterocytozoon bieneusi spores from stool specimens was developed. The primary process of purification of the spores from bacterial contaminants involved Percoll gradient centrifugation followed by additional separation using cesium chloride density gradient centrifugation. ...

journal_title：Journal of clinical microbiology

authors： Kucerova Z,Moura H,Leitch GJ,Sriram R,Bern C,Kawai V,Vargas D,Gilman RH,Ticona E,Vivar A,Visvesvara GS

更新日期：2004-07-01 00:00:00