Simultaneous detection and identification of Candida, Aspergillus, and Cryptococcus species by reverse line blot hybridization.

abstract：:Serratia plymuthica was isolated from the blood and from a central venous catheter tip in a 54-year-old man with alcoholic cirrhosis and clinical signs of sepsis. This was the seventh reported isolate of S. plymuthica from a clinical specimen and the first in which the organism was clearly a significant pathogen. ...

journal_title：Journal of clinical microbiology

authors： Horowitz HW,Nadelman RB,Van Horn KG,Weekes SE,Goyburu L,Wormser GP

更新日期：1987-08-01 00:00:00

abstract：:Clinical presentations for viral respiratory tract infections are often nonspecific, and a rapid, high-throughput laboratory technique that can detect a panel of common viral pathogens is clinically desirable. We evaluated two multiplex reverse transcription-PCR (RT-PCR) products coupled with microarray-based systems ...

journal_title：Journal of clinical microbiology

authors： Li H,McCormac MA,Estes RW,Sefers SE,Dare RK,Chappell JD,Erdman DD,Wright PF,Tang YW

更新日期：2007-07-01 00:00:00

abstract：:Automated DNA sequencing was used to characterize mutations associated with rifampin resistance in a 69-bp region of the gene, rpoB, encoding the beta subunit of RNA polymerase in Mycobacterium tuberculosis. The data confirmed that greater than 90% of rifampin-resistant strains have sequence alterations in this region...

journal_title：Journal of clinical microbiology

authors： Kapur V,Li LL,Iordanescu S,Hamrick MR,Wanger A,Kreiswirth BN,Musser JM

更新日期：1994-04-01 00:00:00

abstract：:Metarhizium viride has been associated with fatal systemic mycoses in chameleons, but subsequent data on mycoses caused by this fungus in reptiles are lacking. The aim of this investigation was therefore to obtain information on the presence of M. viride in reptiles kept as pets in captivity and its association with c...

journal_title：Journal of clinical microbiology

authors： Schmidt V,Klasen L,Schneider J,Hübel J,Pees M

更新日期：2017-03-01 00:00:00

abstract：:Detection of group B streptococcus (GBS) antigen in urine by latex particle agglutination (LPA) may facilitate the rapid diagnosis of GBS sepsis. We sought to compare three commercial LPA assays with specimens that were spiked with type-specific antigen, group-specific antigen, or type III organisms. There were sensit...

journal_title：Journal of clinical microbiology

authors： Ascher DP,Wilson S,Fischer GW

更新日期：1991-12-01 00:00:00

abstract：:The performance of a gp41 assay, created using reagents designed for use with the OpenGene DNA Sequencing System, was evaluated using a panel of plasma samples obtained from enfuvirtide-naive and -experienced human immunodeficiency virus type 1-infected subjects. Resulting sequence data were highly accurate compared t...

journal_title：Journal of clinical microbiology

authors： Marcial M,Lu J,Deeks SG,Ziermann R,Kuritzkes DR

更新日期：2006-09-01 00:00:00

abstract：:The first case of cavitary pulmonary disease caused by Purpureocillium lilacinum is described. The isolate showed atypical microscopic characteristics similar to Acremonium and Fusarium spp., which necessitated molecular identification by sequencing of multiple conserved loci. The patient responded to voriconazole, re...

journal_title：Journal of clinical microbiology

authors： Khan Z,Ahmad S,Al-Ghimlas F,Al-Mutairi S,Joseph L,Chandy R,Sutton DA,Guarro J

更新日期：2012-05-01 00:00:00

abstract：:A prereduced, anaerobically sterilized system of tubed media (PRAS II; Scott Laboratories, Fiskeville, R.I.) was evaluated for accuracy in the identification of anerobic microorganisms. PRAS II was found to be a rapid and accurate identification system for obligate anaerobes which does not require the use of gas cannu...

journal_title：Journal of clinical microbiology

authors： Beaucage CM,Onderdonk AB

更新日期：1982-09-01 00:00:00

abstract：:Baculovirus-expressed foot-and-mouth disease virus (FMDV) nonstructural protein 3AB was used as the antigen in an enzyme-linked immunosorbent assay. This assay allowed the differentiation of vaccinated from infected pigs. Serial studies were performed using sera collected from pigs in the field. Positive reactions wer...

journal_title：Journal of clinical microbiology

authors： Chung WB,Sorensen KJ,Liao PC,Yang PC,Jong MH

更新日期：2002-08-01 00:00:00

abstract：:Three variants of a Corynebacterium diphtheriae subsp. mitis strain belfanti, two toxigenic (phage types 7 and 11) and one nontoxigenic (phage type 7), were simultaneously isolated from the throat of an adult. ...

journal_title：Journal of clinical microbiology

authors： Chang DN,Laughren GS,Chalvardjian NE

更新日期：1978-12-01 00:00:00

abstract：:Invasive aspergillosis (IA) is a life-threatening infection that mainly occurs in immunocompromised patients. Here, we compared the novel Aspergillus-specific galactomannoprotein (GP) enzyme-linked immunosorbent assay (ELISA) (Euroimmun Medizinische Labordiagnostika AG) to the established Platelia Aspergillus galactom...

journal_title：Journal of clinical microbiology

authors： Dichtl K,Seybold U,Ormanns S,Horns H,Wagener J

更新日期：2019-06-25 00:00:00

abstract：:Murex hybrid capture DNA assay (HCS) is a solution hybridization antibody capture assay for detection and quantitation of cytomegalovirus (CMV) DNA in leukocytes. To determine whether CMV HCS is sensitive enough to initiate and monitor antiviral therapy after allogeneic stem cell transplantation (SCT), 51 consecutive ...

journal_title：Journal of clinical microbiology

authors： Hebart H,Gamer D,Loeffler J,Mueller C,Sinzger C,Jahn G,Bader P,Klingebiel T,Kanz L,Einsele H

更新日期：1998-05-01 00:00:00

abstract：:On occasion, a patient may have two or more clinical cultures yielding a coagulase-negative staphylococcus If these multiple isolates have the same phenotype, one might conclude that the same strain was reisolated from the patient, indicating its persistent and pathological presence. We examined the validity of this c...

journal_title：Journal of clinical microbiology

authors： Christensen GD,Parisi JT,Bisno AL,Simpson WA,Beachey EH

更新日期：1983-08-01 00:00:00

abstract：:A monoclonal antibody specific for group D Salmonella antigen 9 was used in an indirect enzyme-linked immunosorbent assay (ELISA) for detecting the antigen in urine specimens collected from patients with clinical typhoid fever in Jakarta, Indonesia. The ELISA had a sensitivity of 95% in identifying patients in whom Sa...

journal_title：Journal of clinical microbiology

authors： Chaicumpa W,Ruangkunaporn Y,Burr D,Chongsa-Nguan M,Echeverria P

更新日期：1992-09-01 00:00:00

abstract：:During a 1-year survey of Shiga toxin-producing Escherichia coli (STEC) prevalence in central France, 2,143 samples were investigated by PCR for Shiga toxin-encoding genes. A total of 330 (70%) of 471 fecal samples collected from healthy cattle at the Clermont-Ferrand slaughterhouse, 47 (11%) of 411 beef samples, 60 (...

journal_title：Journal of clinical microbiology

authors： Pradel N,Livrelli V,De Champs C,Palcoux JB,Reynaud A,Scheutz F,Sirot J,Joly B,Forestier C

更新日期：2000-03-01 00:00:00

abstract：:Fifty-six strains of Borrelia burgdorferi sensu lato, isolated from ticks and vertebrate animals in Missouri, South Carolina, Georgia, Florida, and Texas, were identified and characterized by PCR-restriction fragment length polymorphism (RFLP) analysis of rrf (5S)-rrl (23S) intergenic spacer amplicons. A total of 241 ...

journal_title：Journal of clinical microbiology

authors： Lin T,Oliver JH Jr,Gao L,Kollars TM Jr,Clark KL

更新日期：2001-07-01 00:00:00

abstract：:We developed a strategy to determine the clinical impact associated with errors in clinical microbiology testing. Over a 9-month period, we used a sequential three-stage method to prospectively evaluate 480 consecutive corrected microbiology laboratory reports. The three stages were physician review of the corrected r...

journal_title：Journal of clinical microbiology

authors： Yuan S,Astion ML,Schapiro J,Limaye AP

更新日期：2005-05-01 00:00:00

abstract：:The Porphyromonas gingivalis collagenase-specific serum immunoglobulin A (IgA), IgM, and IgG responses from 20 patients with early-onset periodontitis (EOP), 20 patients with adult periodontitis, (AP), and 20 age- and sex-matched healthy controls were examined by immunoblot analysis. A recombinant collagenase antigen ...

journal_title：Journal of clinical microbiology

authors： Wittstock M,Flemmig TF,Schmidt H,Mutters R,Karch H

更新日期：1996-10-01 00:00:00

abstract：:Trichomonas vaginalis is the most prevalent nonviral sexually transmitted infection worldwide, and improved diagnostic methods are critical for controlling this pathogen. Diagnostic assays that can be used in conjunction with routine chlamydia/gonorrhea nucleic acid-based screening are likely to have the most impact o...

journal_title：Journal of clinical microbiology

authors： Van Der Pol B,Williams JA,Taylor SN,Cammarata CL,Rivers CA,Body BA,Nye M,Fuller D,Schwebke JR,Barnes M,Gaydos CA

更新日期：2014-03-01 00:00:00

abstract：:Animal bite wounds are often infected with bacteria from the animal's oral flora. We report what we believe to be the first case of bacteremia with Bacteroides pyogenes resulting from an infected cat bite. ...

journal_title：Journal of clinical microbiology

authors： Madsen IR,Justesen US

更新日期：2011-08-01 00:00:00

abstract：:A variety of bacterins, vaccines, and antisera retained greater than 90% of their original level of mercurial preservative after lyophilization, and this might influence certain uses of these products. ...

journal_title：Journal of clinical microbiology

authors： Pemberton JR

更新日期：1975-12-01 00:00:00

abstract：:A new molecular assay, based on a rapid DNA extraction protocol, PCR, and hybridization to a specific probe in a nonradioactive microwell plate format was used to detect Mycoplasma pneumoniae in bronchoalveolar fluid specimens. The sensitivity of the assay was determined to be 10 to 100 organisms with M. pneumoniae re...

journal_title：Journal of clinical microbiology

authors： Kessler HH,Dodge DE,Pierer K,Young KK,Liao Y,Santner BI,Eber E,Roeger MG,Stuenzner D,Sixl-Voigt B,Marth E

更新日期：1997-06-01 00:00:00

abstract：:Early and accurate diagnosis is essential for optimal therapeutic outcomes in patients infected with HIV. Currently, none of the commercially available fourth-generation assays differentiate HIV-1 and HIV-2 antibodies (Ab) or the HIV-1 p24 antigen (Ag). The aim of this study was to evaluate the performance of a novel ...

journal_title：Journal of clinical microbiology

authors： Salmona M,Delarue S,Delaugerre C,Simon F,Maylin S

更新日期：2014-01-01 00:00:00

abstract：:Colonization of the gastrointestinal and genitourinary tracts of pregnant women with group B Streptococcus (GBS) can result in vertical transmission to neonates during labor/delivery. GBS infections in neonates can cause severe complications, such as sepsis, meningitis, and pneumonia. Accurate detection is critical be...

journal_title：Journal of clinical microbiology

authors： Shin JH,Pride DT

更新日期：2019-05-24 00:00:00

abstract：:A real-time quantitative PCR assay has been developed to measure human herpesvirus 6 (HHV-6) DNA in biological specimens. The assay sensitivity was 10 copies of DNA per well, with a linear dynamic range of 10 to 10(7) copies of HHV-6 DNA. Intra- and interassay variations were, respectively, 0.88 and 0.8% for samples c...

journal_title：Journal of clinical microbiology

authors： Collot S,Petit B,Bordessoule D,Alain S,Touati M,Denis F,Ranger-Rogez S

更新日期：2002-07-01 00:00:00

abstract：:A commercially available endotoxin assay (CS-TAL) employing a chromogenic peptide and an amebocyte lysate from the Japanese horseshoe crab, Tachypleus tridentatus, gave a positive result with aqueous extracts of all 15 strains of Candida albicans and 1 strain each of Candida tropicalis, Cryptococcus neoformans, and a ...

journal_title：Journal of clinical microbiology

authors： Hodes DS,Heon D,Hass A,Hyatt AC,Hodes HL

更新日期：1987-09-01 00:00:00

abstract：:Antibodies to human papilloma virus (HPV) type 16 (HPV-16) E6 and E7 proteins in serum are markers for HPV-associated invasive cervical carcinoma. We compared two assays, a radioimmunoprecipitation assay with in vitro-translated HPV-16 E6 and E7 proteins and an enzyme-linked immunosorbent assay (ELISA) with E6 and E7 ...

journal_title：Journal of clinical microbiology

authors： Sun Y,Shah KV,Müller M,Muñoz N,Bosch XF,Viscidi RP

更新日期：1994-09-01 00:00:00

abstract：:Superparamagnetic polymer particles precoated with sheep anti-mouse immunoglobulin G were coated with immunoglobulin G2 monoclonal antibodies to the K88 antigen of Escherichia coli (MAb-K88). These immunomagnetic particles (IMP) were used for isolation and identification of K88 antigen-positive (K88+) E. coli. The bac...

journal_title：Journal of clinical microbiology

authors： Lund A,Hellemann AL,Vartdal F

更新日期：1988-12-01 00:00:00

abstract：:A blind volunteer was inoculated in one eye with an isolate of Chlamydia trachomatis in 1961 and followed for 20 years. During this time, many observations were made of his clinical responses to the first inoculation and several subsequent inoculations with the same and other strains, chlamydial shedding, and antibody...

journal_title：Journal of clinical microbiology

authors： Hanna L,Jawetz E,Dawson CR,Thygeson P

更新日期：1982-11-01 00:00:00

abstract：:The coelomycete Colletotrichum gloeosporioides was isolated in pure culture from subcutaneous nodules of the left forearm and elbow of a farmer after traumatic injury. To our knowledge, we report the first case involving this fungus as an etiological agent of subcutaneous infection. The in vitro inhibitory activities ...

journal_title：Journal of clinical microbiology

authors： Guarro J,Svidzinski TE,Zaror L,Forjaz MH,Gené J,Fischman O

更新日期：1998-10-01 00:00:00