Abstract:
:Baculovirus-expressed foot-and-mouth disease virus (FMDV) nonstructural protein 3AB was used as the antigen in an enzyme-linked immunosorbent assay. This assay allowed the differentiation of vaccinated from infected pigs. Serial studies were performed using sera collected from pigs in the field. Positive reactions were found in sera from fattening pigs and sows 16 weeks and 3.5 years postoutbreak, respectively. There was, however, no positive reaction in sows with at least 10 vaccinations. Maternally derived antibodies to the 3AB antigen persisted in piglets up to 13 weeks of age. A high correlation coefficient (r = 0.93) was found between the test results from sow sera and those from their offspring. Therefore, piglet serum was a good substitute for sow serum to monitor the infection status of the dam. The application of this assay to serological surveillance in an FMD eradication program in Taiwan showed that the positive reactors steadily decreased over time in both finishers and sows, indicating that the pig population risk of infection by FMDV has decreased.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Chung WB,Sorensen KJ,Liao PC,Yang PC,Jong MHdoi
10.1128/jcm.40.8.2843-2848.2002subject
Has Abstractpub_date
2002-08-01 00:00:00pages
2843-8issue
8eissn
0095-1137issn
1098-660Xjournal_volume
40pub_type
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