Correlation of oxacillin MIC with mecA gene carriage in coagulase-negative staphylococci.

Abstract:

:The National Committee for Clinical Laboratory Standards has recently changed the oxacillin breakpoint from >/=4 mg/liter to >/=0. 5 mg/liter to detect methicillin-resistant coagulase-negative staphylococci (CoNS) because the previous breakpoint lacked sensitivity. To determine the correlation between the new oxacillin breakpoint and the presence of the mecA gene, 493 CoNS of 11 species were tested. The presence of the mecA gene was determined by PCR, and oxacillin susceptibility was determined by the agar dilution method with Mueller-Hinton agar containing 2% NaCl and oxacillin (0. 125 to 4.0 mg/liter). The new breakpoint correctly classified all CoNS strains with mecA as methicillin resistant and strains of Staphylococcus epidermidis, S. haemolyticus, and S. hominis without mecA as methicillin susceptible. The breakpoint of >/=0.5 mg/liter was not specific for S. cohnii, S. lugdunensis, S. saprophyticus, S. warneri, and S. xylosus, in that it categorized 70 of 74 strains of these species without mecA (94.6%) as methicillin resistant. The results of this study indicate that the new oxacillin breakpoint accurately identifies strains of CoNS with mecA but is not specific for strains of certain species of CoNS without mecA.

journal_name

J Clin Microbiol

authors

Hussain Z,Stoakes L,Massey V,Diagre D,Fitzgerald V,El Sayed S,Lannigan R

doi

10.1128/JCM.38.2.752-754.2000

subject

Has Abstract

pub_date

2000-02-01 00:00:00

pages

752-4

issue

2

eissn

0095-1137

issn

1098-660X

journal_volume

38

pub_type

杂志文章
  • Diagnosis of Herpesvirus hominis infections in a general hospital laboratory.

    abstract::There is a greatly increased interest in Herpesvirus hominis infections especially those of type 2 associated with genital lesions or neonatal disease. Physicians are eager to confirm the clinical impression with a specific virologic diagnosis such as isolation of the agent and its typing, and type-specific antibody r...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.1.3.318-323.1975

    authors: Hanna L,Keshishyan H,Jawetz E,Coleman VR

    更新日期:1975-03-01 00:00:00

  • Competency assessment of microbiology medical laboratory technologists in Ontario, Canada.

    abstract::Accreditation in Ontario, Canada, requires that licensed clinical laboratories participate in external quality assessment (also known as proficiency testing) and perform competency evaluation of their staff. To assess the extent of ongoing competency assessment practices, the Quality Management Program--Laboratory Ser...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01035-14

    authors: Desjardins M,Fleming CA

    更新日期:2014-08-01 00:00:00

  • Outbreak of fungemia among neonates caused by Candida haemulonii resistant to amphotericin B, itraconazole, and fluconazole.

    abstract::The first outbreak of Candida haemulonii fungemia is described. The seven isolates from the blood of four neonates were identified by DNA sequencing of the ribosomal DNA. They were all resistant to amphotericin B, fluconazole, and itraconazole. This report highlights the emergence of C. haemulonii as an opportunistic ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00222-07

    authors: Khan ZU,Al-Sweih NA,Ahmad S,Al-Kazemi N,Khan S,Joseph L,Chandy R

    更新日期:2007-06-01 00:00:00

  • Comparative analysis of agr locus diversification and overall genetic variability among bovine and human Staphylococcus aureus isolates.

    abstract::The accessory gene regulator (agr) is a central system that controls the expression of Staphylococcus aureus virulence factors. In this study, the distribution of agr alleles, defined by agr restriction fragment length polymorphism within agr interference groups, among S. aureus isolates from bovine and human origin w...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.42.3.1265-1269.2004

    authors: Gilot P,van Leeuwen W

    更新日期:2004-03-01 00:00:00

  • babA2- and cagA-positive Helicobacter pylori strains are associated with duodenal ulcer and gastric carcinoma in Brazil.

    abstract::The babA2 and cagA genes were investigated in 208 Brazilian Helicobacter pylori strains. A strong association between babA2 and duodenal ulcer or gastric carcinoma was observed, even after adjusting for confounding factors, such as age, gender, and cagA status. cagA-positive strains were also independently associated ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.41.8.3964-3966.2003

    authors: Oliveira AG,Santos A,Guerra JB,Rocha GA,Rocha AM,Oliveira CA,Cabral MM,Nogueira AM,Queiroz DM

    更新日期:2003-08-01 00:00:00

  • Point-Counterpoint: Meningitis/Encephalitis Syndromic Testing in the Clinical Laboratory.

    abstract::INTRODUCTIONSyndromic panels were first FDA cleared for detection of respiratory pathogens in 2008. Since then, other panels have been approved by the FDA, and most recently, the FilmArray meningitis/encephalitis panel (BioFire, Salt Lake City, UT) has become available. This assay detects 14 targets within 1 h and inc...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00018-18

    authors: Dien Bard J,Alby K

    更新日期:2018-03-26 00:00:00

  • Serological studies of antigenic similarity between Japanese spotted fever rickettsiae and Weil-Felix test antigens.

    abstract::Acute and convalescent-phase sera obtained from 10 patients infected with a Japanese strain of spotted fever group (SFG) rickettsia were tested by the indirect immunoperoxidase test, the Weil-Felix test, an enzyme-linked immunosorbent assay (ELISA), and immunoblotting. By the Weil-Felix test, the reactivity of these s...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.30.9.2441-2446.1992

    authors: Amano K,Hatakeyama H,Okuta M,Suto T,Mahara F

    更新日期:1992-09-01 00:00:00

  • Genotyping reveals the presence of a predominant genotype of Coxiella burnetii in consumer milk products.

    abstract::Real-time PCR shows the widespread presence of Coxiella burnetii DNA in a broad range of commercially available milk and milk products. MLVA genotyping shows that this is the result of the presence of a predominant C. burnetii genotype in the dairy cattle population. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.06831-11

    authors: Tilburg JJ,Roest HJ,Nabuurs-Franssen MH,Horrevorts AM,Klaassen CH

    更新日期:2012-06-01 00:00:00

  • Phage typing set for group C1 and C2 Salmonellae.

    abstract::Fifteen common serotypes representative of group C(1) and C(2)Salmonella were characterized using a single set of phages. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:

    authors: Gershman M

    更新日期:1976-02-01 00:00:00

  • Monitoring Shedding of Five Genotypes of RotaTeq Vaccine Viruses by Genotype-Specific Real-Time Reverse Transcription-PCR Assays.

    abstract::RotaTeq (RV5) is a widely used live attenuated pentavalent rotavirus (RV) vaccine. Although fecal shedding of RV vaccine strains persists for long time periods, it is unclear how each vaccine strain replicates in intestinal tissue and is excreted in stool. To examine this issue, we established RV5 genotype-specific re...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00035-18

    authors: Higashimoto Y,Ihira M,Miyazaki Y,Kuboshiki A,Yoshinaga S,Hiramatsu H,Suzuki R,Miyata M,Miura H,Komoto S,Yukitake J,Taniguchi K,Kawamura Y,Yoshikawa T

    更新日期:2018-05-25 00:00:00

  • Immunoglobulin G antibody capture enzyme-linked immunosorbent assay: a versatile assay for detection of anti-human immunodeficiency virus type 1 and 2 antibodies in body fluids.

    abstract::In tests on specimens of dried blood, saliva, and urine from 55 human immunodeficiency virus (HIV)-seropositive and 55 HIV-seronegative patients, an immunoglobulin G capture enzyme immunoassay for the detection of antibodies to HIV types 1 and 2, GACELISA, gave 109 of 110, 109 of 109, and 109 of 110 correct results, r...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.30.12.3288-3289.1992

    authors: Thongcharoen P,Wasi C,Louisirirotchanakul S,Parry J,Connell J,Mortimer P

    更新日期:1992-12-01 00:00:00

  • Subgenotype analysis of Cryptosporidium isolates from humans, cattle, and zoo ruminants in Portugal.

    abstract::Cryptosporidium parvum and Cryptosporidium hominis isolates from human immunodeficiency virus-infected patients, cattle, and wild ruminants were characterized by PCR and DNA sequencing analysis of the 60-kDa glycoprotein gene. Seven alleles were identified, three corresponding to C. hominis and four corresponding to C...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.41.6.2744-2747.2003

    authors: Alves M,Xiao L,Sulaiman I,Lal AA,Matos O,Antunes F

    更新日期:2003-06-01 00:00:00

  • Progression toward an improved DNA amplification-based typing technique in the study of Mycobacterium tuberculosis epidemiology.

    abstract::While high-copy-number IS6110-based restriction fragment length polymorphism (HCN-RFLP) is the gold standard for typing most Mycobacterium tuberculosis strains, the time taken for culturing and low throughput make it impractical for large-scale prospective typing of large numbers of isolates. The development of a new ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01428-05

    authors: Gopaul KK,Brown TJ,Gibson AL,Yates MD,Drobniewski FA

    更新日期:2006-07-01 00:00:00

  • Comparison of formalin-ethyl ether sedimentation, formalin-ethyl acetate sedimentation, and zinc sulfate flotation techniques for detection of intestinal parasites.

    abstract::Formalin-ethyl ether sedimentation, Formalin-ethyl acetate sedimentation, and zinc sulfate flotation techniques were compared using over 250 clinical parasitology specimens. Fifty positive specimens were identified, and a variety of parasites, including amoebae, flagellates, cestodes, nematodes, and trematodes, were e...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.13.5.882-884.1981

    authors: Truant AL,Elliott SH,Kelly MT,Smith JH

    更新日期:1981-05-01 00:00:00

  • Diminished treatment response of periodontally diseased patients infected with the JP2 clone of Aggregatibacter (Actinobacillus) actinomycetemcomitans.

    abstract::This longitudinal study evaluated the response to periodontal treatment by subjects infected with either JP2 (n = 25) or non-JP2 (n = 25) Aggregatibacter (Actinobacillus) actinomycetemcomitans. Participants were treated during the first 4 months by receiving (i) scaling and root planing, (ii) systemic antibiotic thera...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00338-09

    authors: Cortelli SC,Costa FO,Kawai T,Aquino DR,Franco GC,Ohara K,Roman-Torres CV,Cortelli JR

    更新日期:2009-07-01 00:00:00

  • Sodium polyanethole sulfonate as an inhibitor of activation of complement function in blood culture systems.

    abstract::Sodium polyanethole sulfonate (SPS; trade name, Liquoid) is a constituent in culture media used to grow bacteria from blood samples from patients suspected of bacteremia. SPS prevents the killing of bacteria by innate cellular and humoral factors. We analyzed the effect of SPS on the three complement activation pathwa...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01985-09

    authors: Palarasah Y,Skjoedt MO,Vitved L,Andersen TE,Skjoedt K,Koch C

    更新日期:2010-03-01 00:00:00

  • Genotypic diversity of mutans streptococci in Brazilian nursery children suggests horizontal transmission.

    abstract::Streptococcus mutans strains were isolated from cohorts of Brazilian nursery school children and genotyped by arbitrarily primed PCR and restriction fragment length polymorphism analysis. Of 24 children with two to five S. mutans isolates, 29% carried two or more genotypes. The presence of matching genotypes of S. mut...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.39.6.2313-2316.2001

    authors: Mattos-Graner RO,Li Y,Caufield PW,Duncan M,Smith DJ

    更新日期:2001-06-01 00:00:00

  • Detection of encapsulation in Staphylococcus aureus by use of antiserum agar.

    abstract::We examined an antiserum agar method to study its reliability in screening Staphylococcus aureus strains for capsule production. The encapsulated S. aureus Smith diffuse strain was compared with its nonencapsulated variant, Smith compact, in CCY medium containing 0.5% NaCl and 5.0% Smith diffuse rabbit antiserum. A ha...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.20.2.141-144.1984

    authors: West TE,Apicella MA

    更新日期:1984-08-01 00:00:00

  • Rapid detection of New Delhi metallo-β-lactamase gene and variants coding for carbapenemases with different activities by use of a PCR-based in vitro protein expression method.

    abstract::New Delhi metallo-β-lactamase (NDM)-producing bacteria are considered potential global health threats. It is necessary to monitor NDM-1 and its variants in clinical isolates in order to understand the NDM-1 epidemic and the impact of its variants on β-lactam resistance. To reduce the lengthy time needed for cloning an...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.03363-13

    authors: Huang L,Hu X,Zhou M,Yang Y,Qiao J,Wang D,Yu J,Cui Z,Zhang Z,Zhang XE,Wei H

    更新日期:2014-06-01 00:00:00

  • Antigen(s) responsible for immunoglobulin G responses specific for the acute stage of Toxoplasma infection in humans.

    abstract::An agglutination test for immunoglobulin G (IgG) Toxoplasma antibodies with acetone-fixed tachyzoites (AC antigens; AC agglutination test) was positive only with sera from patients during the acute stage of their infection. In contrast, when the test was performed with Formalin-fixed tachyzoites (HS antigens; HS agglu...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.26.5.901-905.1988

    authors: Suzuki Y,Thulliez P,Desmonts G,Remington JS

    更新日期:1988-05-01 00:00:00

  • Species-specific monoclonal antibodies to Rickettsia japonica, a newly identified spotted fever group rickettsia.

    abstract::A total of 192 hybridomas were developed from mice immunized with Rickettsia japonica, a newly identified spotted fever group rickettsia pathogenic for humans. Of these hybridomas, 101 were species specific, 37 were spotted fever group reactive, and the other 54 were also reactive with one or more of the other pathoge...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.28.6.1177-1180.1990

    authors: Uchiyama T,Uchida T,Walker DH

    更新日期:1990-06-01 00:00:00

  • Rapid purification of hepatitis B virus DNA from serum.

    abstract::We describe two rapid, simple, and reliable procedures for routine purification of hepatitis B virus (HBV) DNA from serum. HBV DNA could be purified from 24 serum samples in 1.5 to 2 h and was recovered in the initial reaction vessel. Both procedures have in common that HBV DNA is complexed with silica particles in th...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.29.9.1804-1811.1991

    authors: Boom R,Sol CJ,Heijtink R,Wertheim-van Dillen PM,van der Noordaa J

    更新日期:1991-09-01 00:00:00

  • Impact of genomic sequence variability on quantitative PCR assays for diagnosis of polyomavirus BK infection.

    abstract::Knowledge of polyomavirus BK (BKV) genomic diversity has greatly expanded. The implications of BKV DNA sequence variation for the performance of molecular diagnostic assays is not well studied. We analyzed 184 publically available VP-1 sequences encompassing the BKV genomic region targeted by an in-house quantitative ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01230-11

    authors: Randhawa P,Kant J,Shapiro R,Tan H,Basu A,Luo C

    更新日期:2011-12-01 00:00:00

  • Fatal acute cellulitis due to Neisseria meningitidis.

    abstract::We describe the first fatal evolution of cellulitis due to Neisseria meningitidis serogroup Y involving an 85-year-old woman. She presented with an extensive cellulitis of the left side of the face, neck, and thorax and septic shock. In spite of active antibiotic therapy, evolution was rapidly fatal. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.41.8.3996-3997.2003

    authors: Cartolano GL,Barbier C,Arnoult L,Simon D,Ricome JL,Hayon J

    更新日期:2003-08-01 00:00:00

  • Sensitive detection of Borrelia burgdorferi sensu lato DNA and differentiation of Borrelia species by LightCycler PCR.

    abstract::In order to differentiate species within the Borrelia burgdorferi sensu lato complex, LightCyler PCR and melting-curve analysis of the amplicons of two genes with intraspecies variability, the p66 gene and the recA gene, were performed. It was demonstrated that nested LightCycler PCR amplification of p66 is more sensi...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.39.7.2663-2667.2001

    authors: Mommert S,Gutzmer R,Kapp A,Werfel T

    更新日期:2001-07-01 00:00:00

  • Evaluation of the new chromogenic medium Candida ID 2 for isolation and identification of Candida albicans and other medically important Candida species.

    abstract::The usefulness of Candida ID 2 (CAID2) reformulated medium (bioMérieux, France) has been compared with that of the former Candida ID (CAID; bioMérieux), Albicans ID 2 (ALB2; bioMérieux), and CHROMagar Candida (CAC; Chromagar, France) chromogenic media for the isolation and presumptive identification of clinically rele...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00213-06

    authors: Eraso E,Moragues MD,Villar-Vidal M,Sahand IH,González-Gómez N,Pontón J,Quindós G

    更新日期:2006-09-01 00:00:00

  • PCR methods for rapid identification and characterization of Actinobacillus seminis strains.

    abstract::Twenty-four isolates of Actinobacillus seminis were typed by PCR ribotyping, repetitive extragenic palindromic element (REP)-based PCR, and enterobacterial repetitive intergenic consensus (ERIC)-based PCR. Five types were distinguished by REP-PCR, and nine types were distinguished by ERIC-PCR. PCR ribotyping produced ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.36.3.814-817.1998

    authors: Appuhamy S,Coote JG,Low JC,Parton R

    更新日期:1998-03-01 00:00:00

  • Evaluation of Staph ID 32 system and Staph-Zym system for identification of coagulase-negative staphylococci.

    abstract::The purpose of the investigation was to evaluate two commercially available identification systems: a new modification of the Staph-Zym system (Rosco, Tåstrup, Denmark) and the Staph ID 32 API system (API System, BioMérieux, Paris, France). A local standard method to be used in routine laboratories was also evaluated....

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.33.5.1150-1153.1995

    authors: Renneberg J,Rieneck K,Gutschik E

    更新日期:1995-05-01 00:00:00

  • Clonal reproduction and limited dispersal in an environmental population of Cryptococcus neoformans var gattii isolates from Australia.

    abstract::Cryptococcus neoformans var. gattii is a causative agent of cryptococcosis and is thought to have a specific ecological association with a number of Eucalyptus species in Australia. However, the role that the tree plays in the life cycle of the fungus and the nature of the infectious propagule are not well understood....

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.41.2.703-711.2003

    authors: Halliday CL,Carter DA

    更新日期:2003-02-01 00:00:00

  • Presumptive identification of Candida kefyr on levine formulation of eosin methylene blue agar.

    abstract::Three hundred thirty-one yeast and yeast-like isolates were cultivated on eosin methylene blue agar. While the sensitivity rate for Candida kefyr isolates producing a metallic green sheen was 81.8%, the high positive predictive value (100%) for yeast isolates with this phenotype belonging to C. kefyr suggests that the...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.40.11.4281-4284.2002

    authors: Munson EL,Troy DR,Weber JK,Messer SA,Pfaller MA

    更新日期:2002-11-01 00:00:00