babA2- and cagA-positive Helicobacter pylori strains are associated with duodenal ulcer and gastric carcinoma in Brazil.

abstract：:We studied the plasmid profiles of 136 Shigella isolates in Bangladesh to determine whether plasmid profiles could be used for differentiation of strains for epidemiological studies. Many different plasmid patterns were observed within each species, indicating that many genetically different strains of Shigella are re...

journal_title：Journal of clinical microbiology

authors： Tacket CO,Shahid N,Huq MI,Alim AR,Cohen ML

更新日期：1984-08-01 00:00:00

abstract：:Antigenic sites on the nucleocapsid (N) protein of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) were mapped by Pepscan analysis with overlapping peptides that span the N protein sequence. Two major immunodominant epitopes located in the C-terminal region (amino acids [aa] 362 to 412) and middle regi...

journal_title：Journal of clinical microbiology

authors： He Y,Zhou Y,Wu H,Kou Z,Liu S,Jiang S

更新日期：2004-11-01 00:00:00

abstract：:Potomac horse fever, a recently recognized disease of equines, characterized by high fever, leukopenia, and a profuse diarrhea, was studied for its etiology. An Ehrlichia organism was isolated in equine macrophage-fibroblast cell cultures and mouse macrophage cell cultures from the mononuclear cells of blood of infect...

journal_title：Journal of clinical microbiology

authors： Dutta SK,Myrup AC,Rice RM,Robl MG,Hammond RC

更新日期：1985-08-01 00:00:00

abstract：:From January 1995 to May 1998, 57 episodes of bacteremia due to viridans group streptococci were identified in 50 febrile neutropenic patients with hematologic malignancies. Four patients experienced two separate episodes of streptococcal bacteremia, and one patient had four separate episodes of streptococcal bacterem...

journal_title：Journal of clinical microbiology

authors： Wisplinghoff H,Reinert RR,Cornely O,Seifert H

更新日期：1999-06-01 00:00:00

abstract：:The in vitro susceptibilities of 66 molecularly identified strains of the Mucorales to eight antifungals (amphotericin B, terbinafine, itraconazole, posaconazole, voriconazole, caspofungin, micafungin, and 5-fluorocytosine) were tested. Molecular phylogeny was reconstructed based on the nuclear ribosomal large subunit...

journal_title：Journal of clinical microbiology

authors： Vitale RG,de Hoog GS,Schwarz P,Dannaoui E,Deng S,Machouart M,Voigt K,van de Sande WW,Dolatabadi S,Meis JF,Walther G

更新日期：2012-01-01 00:00:00

abstract：:Sixteen metabolically deficient dwarf-colony mutants of Escherichia coli were isolated from urine culture and represented about 2% of all E. coli isolated during the same period. In 14 cases, mutants were isolated from debilitated patients: elderly persons or patients in the terminal stages of a chronic disease. In 15...

journal_title：Journal of clinical microbiology

authors： Borderon E,Horodniceanu T

更新日期：1978-12-01 00:00:00

abstract：:Campylobacter jejuni (20 strains) and Campylobacter coli (12 strains) were assigned to four biovars for each species based on phenotypic tests that were easy to perform and interpret. The resulting biotyping schemes offer a greater degree of distinction among C. jejuni and C. coli strains than any of the other biotypi...

journal_title：Journal of clinical microbiology

authors： Roop RM 2nd,Smibert RM,Krieg NR

更新日期：1984-11-01 00:00:00

abstract：:A rapid screening test has been developed to detect heat-labile enterotoxin produced by individual colonies of Escherichia coli or Vibrio cholerae growing on media solidified with agar. The applicability of this method for isolating tox mutants of E. coli and V. cholerae has been demonstrated. ...

journal_title：Journal of clinical microbiology

authors： Bramucci MG,Holmes RK

更新日期：1978-08-01 00:00:00

abstract：:Bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV) are orbiviruses that infect both livestock and wild ruminants. Antigenic cross-reactivity between BTV and EHDV often results in serologic misdiagnosis. Competitive enzyme-linked immunosorbent assays (c-ELISAs) show increased sensitivity and specific...

journal_title：Journal of clinical microbiology

authors： Mecham JO,Wilson WC

更新日期：2004-02-01 00:00:00

abstract：:We prospectively studied 156 patients with a diagnosis of community-acquired pneumonia requiring admission. Several respiratory specimens were obtained for the detection of Chlamydia pneumoniae by cell culture and PCR. Three serum samples were obtained from each patient. Serological diagnosis of a C. pneumoniae infect...

journal_title：Journal of clinical microbiology

authors： Verkooyen RP,Willemse D,Hiep-van Casteren SC,Joulandan SA,Snijder RJ,van den Bosch JM,van Helden HP,Peeters MF,Verbrugh HA

更新日期：1998-08-01 00:00:00

abstract：:Eighty-seven strains of Pseudomonas aeruginosa were typed by random amplification of polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE) of macrorestriction fragments. Stains were clustered on the basis of interpretative criteria as presented previously for the PFGE analysis. Clusters of strains were al...

journal_title：Journal of clinical microbiology

authors： Renders N,Römling U,Verbrugh H,van Belkum A

更新日期：1996-12-01 00:00:00

abstract：:In this study, we compared a recently developed PCR-restriction fragment length polymorphism (PCR-RFLP) assay with pulsed-field gel electrophoresis (PFGE) using three different Shiga toxin-producing Escherichia coli (STEC) strains to understand whether repeated subculture in vitro and prolonged storage at room tempera...

journal_title：Journal of clinical microbiology

authors： Shima K,Wu Y,Sugimoto N,Asakura M,Nishimura K,Yamasaki S

更新日期：2006-11-01 00:00:00

abstract：:Tracking the spread of noroviruses during outbreaks of gastroenteritis is hampered by the lack of sequence diversity in those regions of the genome chosen for virus detection and characterization. Sequence analysis of regions of the genes encoding the RNA-dependent RNA polymerase and the S domain of the capsid does no...

journal_title：Journal of clinical microbiology

authors： Xerry J,Gallimore CI,Iturriza-Gómara M,Allen DJ,Gray JJ

更新日期：2008-03-01 00:00:00

abstract：:We have investigated 677 Shiga toxin-producing Escherichia coli (STEC) strains from humans to determine their serotypes, virulence genes, and clinical signs in patients. Six different Shiga toxin types (1, 1c, 2, 2c, 2d, and 2e) were distributed in the STEC strains. Intimin (eae) genes were present in 62.6% of the str...

journal_title：Journal of clinical microbiology

authors： Beutin L,Krause G,Zimmermann S,Kaulfuss S,Gleier K

更新日期：2004-03-01 00:00:00

abstract：:The purpose of our study was the molecular characterization of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated in 21 hospitals in the Czech Republic in the period 2000-2002 and comparison with previous results from 1996-1997. Strains were analyzed by pulsed-field gel electrophoresis (PFGE) of SmaI ...

journal_title：Journal of clinical microbiology

authors： Melter O,Aires de Sousa M,Urbásková P,Jakubů V,Zemlicková H,de Lencastre H

更新日期：2003-11-01 00:00:00

abstract：:A recombinant protein (p41-G) of an antigenic region of flagellin was used in a standard and amplified enzyme-linked immunosorbent assay (ELISA) to detect antibodies to Borrelia burgdorferi, the causative agent of Lyme borreliosis. Comparable sensitivities (88 to 94%) were noted when sera from 17 persons who had eryth...

journal_title：Journal of clinical microbiology

authors： Magnarelli LA,Fikrig E,Berland R,Anderson JF,Flavell RA

更新日期：1992-12-01 00:00:00

abstract：:Comparative evaluations of immune status for rubella virus are described for enzyme-linked immunosorbent assay, hemagglutination inhibition, and indirect hemagglutination. A 92.1% agreement between enzyme-linked immunosorbent assay and indirect hemagglutination assay was demonstrated for rubella immune status. Enzyme-...

journal_title：Journal of clinical microbiology

authors： Truant AL,Barksdale BL,Huber TW,Elliott LB

更新日期：1983-01-01 00:00:00

abstract：:Genotyping and subtyping of 225 samples with hepatitis C virus (HCV) genotype 1, 2, 3, or 6 infection were done with Versant LiPA 2.0 and Abbott RealTime HCV Genotype (GT) II by using direct sequencing of the NS5B and 5' untranslated regions as the reference standards. The concordance rates were >99.2% for genotypes a...

journal_title：Journal of clinical microbiology

authors： Liu CH,Liang CC,Liu CJ,Lin CL,Su TH,Yang HC,Chen PJ,Chen DS,Kao JH

更新日期：2015-05-01 00:00:00

abstract：:Eighty-four pneumococci with various MICs of penicillin (38 with MICs of less than or equal to 0.06 micrograms/ml [susceptible], 35 with MICs of 0.12 to 1.0 micrograms/ml [relatively resistant], and 11 with MICs of greater than 1.0 micrograms/ml [resistant] ) were screened by a disk diffusion test using oxacillin and ...

journal_title：Journal of clinical microbiology

authors： Swenson JM,Hill BC,Thornsberry C

更新日期：1986-11-01 00:00:00

abstract：:Indirect and double-antibody sandwich enzyme-linked immunosorbent assay techniques were compared for serotyping 42 herpes simplex virus isolates. Both procedures gave similar results, which were in complete agreement with those obtained by inhibition of the indirect hemagglutination test. The indirect technique proved...

journal_title：Journal of clinical microbiology

authors： Gerna G,Battaglia M,Revello MG,Gerna MT

更新日期：1983-05-01 00:00:00

abstract：:To identify Trichinella antigens suitable for high-specificity and high-sensitivity serodiagnosis of human trichinellosis, we evaluated assays using four antigens: (i) crude first-stage larval extract (CLE), (ii) O-deglycosylated CLE, (iii) tyvelose-bearing antigens (Trichinella spiralis larva group 1 [TSL-1] antigens...

journal_title：Journal of clinical microbiology

authors： Escalante M,Romarís F,Rodríguez M,Rodríguez E,Leiro J,Gárate MT,Ubeira FM

更新日期：2004-09-01 00:00:00

abstract：:Evaluation of previously described chemically defined media for the growth of Legionella pneumophila showed that these media supported poor growth of several strains of L. pneumophila and did not support growth of certain of the Legionella species described later. Growth was stimulated by the dialysate from yeast extr...

journal_title：Journal of clinical microbiology

authors： Pine L,Franzus MJ,Malcolm GB

更新日期：1986-01-01 00:00:00

abstract：:To evaluate the ability of the Amplicor MTB Assay to detect Mycobacterium tuberculosis complex (MTBC) organisms in BACTEC 12B broth cultures, 249 cultures with a growth index (GI) of > or = 20 from 160 patients were tested retrospectively. Specimens were processed by standard methods, and then BACTEC 12B vials and Mid...

journal_title：Journal of clinical microbiology

authors： Smith MB,Bergmann JS,Woods GL

更新日期：1997-04-01 00:00:00

abstract：:We compared a new colony hybridization assay with an established enzyme-linked immunosorbent assay for detection of enterotoxigenic Escherichia coli (ETEC) expressing colonization factor antigen I (CFA/I). The tests were applied to 135 human ETEC strains. Of these isolates, 30 had previously been characterized for CFA...

journal_title：Journal of clinical microbiology

authors： Grewal HM,Sommerfelt H,Gaastra W,Svennerholm AM,Bhan MK,Hamers AM,Kumar R,Wiklund G,Bjorvatn B

更新日期：1990-10-01 00:00:00

abstract：:The human erythrocyte receptor which mediates mannose-resistant hemagglutination by enterotoxigenic Escherichia coli possessing colonization factor antigen II is not universally distributed among donors. Mannose-resistant hemagglutination-positive erythrocytes are more common among black donors than nonblack donors; t...

journal_title：Journal of clinical microbiology

authors： Evans DJ Jr,Evans DG,Diaz SR,Graham DY

更新日期：1988-09-01 00:00:00

abstract：:Lymphadenopathy (LN) is the most common clinical manifestation of acute acquired toxoplasma infection in humans. The diagnosis of toxoplasmic lymphadenitis (TL) is established by serological methods and/or lymph node biopsy. In the United States, the differential agglutination (of acetone [AC]-fixed versus that of for...

journal_title：Journal of clinical microbiology

authors： Montoya JG,Berry A,Rosso F,Remington JS

更新日期：2007-05-01 00:00:00

abstract：:Few studies have assessed the time to blood culture positivity as a predictor of clinical outcome in bloodstream infections (BSIs). The purpose of this study was to evaluate the time to positivity (TTP) of blood cultures in patients with Staphylococcus aureus BSIs and to assess its impact on clinical outcome. We perfo...

journal_title：Journal of clinical microbiology

authors： Marra AR,Edmond MB,Forbes BA,Wenzel RP,Bearman GM

更新日期：2006-04-01 00:00:00

abstract：:The use of a new commercial separation column on whole test serum improved the method for detecting immunoglobulin M antibody to Epstein-Barr virus. The efficiency of this product in absorbing interfering immunoglobulin G was similar to that of another absorption product, staphylococcal protein A, but it has the advan...

journal_title：Journal of clinical microbiology

authors： Sumaya CV,Ench Y,Carrillo MA,Pope RM

更新日期：1984-08-01 00:00:00

abstract：:A biotyping scheme using improved media and methods for the detection of hippurate hydrolysis, rapid H2S production, and DNA hydrolysis was applied to 1,826 cultures of Campylobacter jejuni, Campylobacter coli and "Campylobacter laridis" isolates from human and nonhuman sources. Four biotypes were identified among C. ...

journal_title：Journal of clinical microbiology

authors： Lior H

更新日期：1984-10-01 00:00:00

abstract：:The new colorimetric Vitek 2 YST card was evaluated for identification of yeasts (136 strains) with respect to the influence of different source media. The Vitek 2 YST card achieved satisfactory results for all yeast species tested, with the exception of Candida guilliermondii, Candida norvegensis, Candida parapsilosi...

journal_title：Journal of clinical microbiology

authors： Valenza G,Strasen J,Schäfer F,Frosch M,Kurzai O,Abele-Horn M

更新日期：2008-11-01 00:00:00