Evaluation of Trichinella spiralis larva group 1 antigens for serodiagnosis of human trichinellosis.

Abstract:

:To identify Trichinella antigens suitable for high-specificity and high-sensitivity serodiagnosis of human trichinellosis, we evaluated assays using four antigens: (i) crude first-stage larval extract (CLE), (ii) O-deglycosylated CLE, (iii) tyvelose-bearing antigens (Trichinella spiralis larva group 1 [TSL-1] antigens) purified by US4 affinity chromatography and coupled directly to enzyme-linked immunosorbent assay (ELISA) plates (pTSL-1 antigens), and (iv) TSL-1 antigens immobilized on ELISA plates with the monoclonal antibody (MAb) US4 (cTSL-1 antigens). Assays using these antigens were compared by analysis of sera from healthy individuals (n = 224) (group 1), individuals with noninfectious intestinal pathologies (n = 114) (group 2), individuals with other parasitic infections (n = 107) (group 3), and individuals with confirmed trichinellosis (n = 42) (group 4). Our results indicate that capture ELISA using cTSL-1 antigens is the most effective method for serodiagnosis of human trichinellosis; this was the only method showing 100% specificity and 100% sensitivity at the patent stage of the infection, and it was also the most sensitive for sera obtained prior to patency in indirect immunofluorescence (IIF). Indirect ELISA with pTSL-1 antigens was also 100% specific but was slightly less sensitive, particularly with sera obtained before IIF patency. Inhibition ELISA with MAb US4 indicated (i) that in Trichinella-infected patients the immune response to TSL-1 antigens is directed mostly against tyvelose-containing epitopes (mean of 84.2% of total anti-TSL-1 immunoglobulin G1 [IgG1] antibody response [range, 51.3 to 97.6%]) and (ii) that in most individuals a large proportion of anti-CLE IgG1 antibodies (mean, 49.5%; range, 7.3 to 92.6%) are directed against tyvelose epitopes.

journal_name

J Clin Microbiol

authors

Escalante M,Romarís F,Rodríguez M,Rodríguez E,Leiro J,Gárate MT,Ubeira FM

doi

10.1128/JCM.42.9.4060-4066.2004

subject

Has Abstract

pub_date

2004-09-01 00:00:00

pages

4060-6

issue

9

eissn

0095-1137

issn

1098-660X

pii

42/9/4060

journal_volume

42

pub_type

杂志文章
  • Diagnostic Accuracy of Stool Xpert MTB/RIF for Detection of Pulmonary Tuberculosis in Children: a Systematic Review and Meta-analysis.

    abstract::Invasive collection methods are often required to obtain samples for the microbiological evaluation of children with presumptive pulmonary tuberculosis (PTB). Nucleic acid amplification testing of easier-to-collect stool samples could be a noninvasive method of diagnosing PTB. We conducted a systematic review and meta...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章,meta分析

    doi:10.1128/JCM.02057-18

    authors: MacLean E,Sulis G,Denkinger CM,Johnston JC,Pai M,Ahmad Khan F

    更新日期:2019-05-24 00:00:00

  • Four-day incubation period for blood culture bottles processed with the Difco ESP blood culture system.

    abstract::Blood culture records from 1994 to 1995 from five U.S. medical centers all using the Difco ESP continuous monitoring blood culture system were reviewed retrospectively. Among a total of 7,362 isolates of bacteria and yeasts, only 0.1% of possibly significant isolates would have been missed had blood cultures been rout...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.35.5.1290-1292.1997

    authors: Doern GV,Brueggemann AB,Dunne WM,Jenkins SG,Halstead DC,McLaughlin JC

    更新日期:1997-05-01 00:00:00

  • Clinical and molecular epidemiology of Staphylococcus argenteus infections in Thailand.

    abstract::Molecular typing of 246 Staphylococcus aureus isolates from unselected patients in Thailand showed that 10 (4.1%) were actually Staphylococcus argenteus. Contrary to the suggestion that S. argenteus is less virulent than S. aureus, we demonstrated comparable rates of morbidity, death, and health care-associated infect...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.03049-14

    authors: Thaipadungpanit J,Amornchai P,Nickerson EK,Wongsuvan G,Wuthiekanun V,Limmathurotsakul D,Peacock SJ

    更新日期:2015-03-01 00:00:00

  • Proposed quality control and interpretive criteria for disk diffusion susceptibility testing with enoxacin.

    abstract::The standardized disk diffusion test, in which a 10-micrograms enoxacin disk is used, was performed and microbroth dilution MICs were determined to establish individual test control values with Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, and S. aureus ATCC 29213. I...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.21.3.332-334.1985

    authors: Rudrik JT,Cavalieri SJ,Britt EM

    更新日期:1985-03-01 00:00:00

  • Mycobacterium tuberculosis strains of the modern sublineage of the Beijing family are more likely to display increased virulence than strains of the ancient sublineage.

    abstract::Strains of the Beijing genotype family of Mycobacterium tuberculosis are a cause of particular concern because of their increasing dissemination in the world and their association with drug resistance. Phylogenetically, this family includes distinct ancient and modern sublineages. The modern strains, contrary to the a...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00498-14

    authors: Ribeiro SC,Gomes LL,Amaral EP,Andrade MR,Almeida FM,Rezende AL,Lanes VR,Carvalho EC,Suffys PN,Mokrousov I,Lasunskaia EB

    更新日期:2014-07-01 00:00:00

  • Atypical isolates of Brucella abortus from Canada and the United States characterized as dye sensitive with M antigen dominant.

    abstract::A total of 41 Brucella isolates, examined by standard biotyping procedures, were found to be similar to Brucella abortus biovar 2 in dye sensitivity but had a dominant M antigen. Oxidative metabolic tests performed on 39 of the isolates confirmed them as B. abortus. Additional biochemical and bacteriophage susceptibil...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.25.4.698-701.1987

    authors: Ewalt DR,Forbes LB

    更新日期:1987-04-01 00:00:00

  • Detection of cytomegalovirus DNA in human specimens by LightCycler PCR.

    abstract::Detection of human cytomegalovirus (CMV) DNA in clinical specimens is considered a cornerstone in the diagnosis of CMV disease. The aim of this study was to evaluate a newly designed LightCycler-based quantitative CMV PCR. Specimens of human origin (n = 200) were tested using the LightCycler PCR, the quantitative COBA...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.38.11.4006-4009.2000

    authors: Schaade L,Kockelkorn P,Ritter K,Kleines M

    更新日期:2000-11-01 00:00:00

  • Detection of binding antibodies to native and recombinant human immunodeficiency virus type 1 envelope glycoproteins following recombinant gp160 immunization measured by flow cytometry and enzyme immunoassays. The AIDS Vaccine Clinical Trials Network.

    abstract::The ability of antibody induced by vaccination with recombinant gp160 (rgp160) to bind to native and recombinant human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins was measured. Thirty-three HIV-1-seronegative healthy adult volunteers were injected four times with 40 or 80 micrograms of an HIV-1LAV env...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.30.10.2606-2612.1992

    authors: Gorse GJ,Frey SE,Newman FK,Belshe RB

    更新日期:1992-10-01 00:00:00

  • Heated versus unheated sera in the hemagglutination treponemal test for syphilis.

    abstract::Sera (920) were tested to evaluate the use of heated versus unheated sera in the hemagglutination treponemal test for syphilis. The heated and unheated samples were tested on the same day with the hemagglutination treponemal test for syphilis kit according to the manufacturer's protocol. Agreement of results between t...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.15.2.337-338.1982

    authors: Hambie EA,Larsen SA,Perryman MW,Pettit DE,Feeley JC

    更新日期:1982-02-01 00:00:00

  • A Fully Automated Multiplex Assay for Diagnosis of Lyme Disease with High Specificity and Improved Early Sensitivity.

    abstract::Lyme borreliosis is a tick-borne disease caused by the Borrelia burgdorferisensu lato complex. Bio-Rad Laboratories has developed a fully automated multiplex bead-based assay for the detection of IgM and IgG antibodies to B. burgdorferi The BioPlex 2200 Lyme Total assay exhibits an improved rate of seropositivity in p...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01785-19

    authors: Hahm JB,Breneman JW 4th,Liu J,Rabkina S,Zheng W,Zhou S,Walker RP,Kaul R

    更新日期:2020-04-23 00:00:00

  • Comparative sequencing of the serine-aspartate repeat-encoding region of the clumping factor B gene (clfB) for resolution within clonal groups of Staphylococcus aureus.

    abstract::Molecular techniques such as spa typing and multilocus sequence typing use DNA sequence data for differentiating Staphylococcus aureus isolates. Although spa typing is capable of detecting both genetic micro- and macrovariation, it has less discriminatory power than the more labor-intensive pulsed-field gel electropho...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.43.8.3985-3994.2005

    authors: Koreen L,Ramaswamy SV,Naidich S,Koreen IV,Graff GR,Graviss EA,Kreiswirth BN

    更新日期:2005-08-01 00:00:00

  • Catheter-related bloodstream infection by Tsukamurella inchonensis in an immunocompromised patient.

    abstract::We report a case of catheter-related bloodstream infection by Tsukamurella inchonensis, identified using 16S rRNA gene sequencing, in a patient with myelofibrosis who underwent a bone marrow transplant. Tsukamurella species infections are rare. To our knowledge, this is the first case of T. inchonensis bloodstream inf...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00421-14

    authors: Takebe I,Sawabe E,Ohkusu K,Tojo N,Tohda S

    更新日期:2014-06-01 00:00:00

  • Novel gelatin particle agglutination test for serodiagnosis of leprosy in the field.

    abstract::We developed a novel gelatin particle agglutination test (MLPA) for the serodiagnosis of leprosy; this test is especially useful for clinical practice and epidemiological surveys of leprosy in countries in which the disease is endemic. The antigen used in the test is the chemically synthesized trisaccharide moiety of ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.28.3.525-529.1990

    authors: Izumi S,Fujiwara T,Ikeda M,Nishimura Y,Sugiyama K,Kawatsu K

    更新日期:1990-03-01 00:00:00

  • Isolation of infectious bovine rhinotracheitis virus from Mustelidae.

    abstract::Herpesviruses isolated from either domesticated or wild carnivores should be immunologically compared with known viruses of this group which could have been included in the diet of the animal before being considered to be previously undescribed herpesviruses native to the carnivore in question. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.1.1.112-113.1975

    authors: Porter DD,Larsen AE,Cox NA

    更新日期:1975-01-01 00:00:00

  • Cloning and characterization of a nonhemolytic phospholipase C gene from Burkholderia pseudomallei.

    abstract::We cloned and characterized a phosphatidylcholine-hydrolyzing phospholipase C (PC-PLC) gene from Burkholderia pseudomallei. DNA sequence analysis of the gene indicated an open reading frame coding for 700 amino acids with a 34-amino-acid signal peptide. When cleaved, this yields a secreted 73-kDa mature protein. The d...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.37.11.3742-3745.1999

    authors: Korbsrisate S,Suwanasai N,Leelaporn A,Ezaki T,Kawamura Y,Sarasombath S

    更新日期:1999-11-01 00:00:00

  • Evaluation of commercial enzyme immunoassays for detection of hepatitis delta antigen and anti-hepatitis delta virus (HDV) and immunoglobulin M anti-HDV antibodies.

    abstract::Panels of hepatitis B virus surface antigen-positive sera from drug abusers were used to evaluate 14 commercial enzyme immunoassays from six companies for detecting hepatitis delta virus (HDV) markers. For detecting hepatitis delta virus antigen (HDAg), the Wellcome, Pasteur and Noctech assays had 100% sensitivity for...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.29.9.1873-1876.1991

    authors: Shattock AG,Morris MC

    更新日期:1991-09-01 00:00:00

  • Evaluation of real-time PCR laboratory-developed tests using analyte-specific reagents for cytomegalovirus quantification.

    abstract::Viral load testing for cytomegalovirus (CMV) has become the standard for the diagnosis of infection and monitoring of therapy at many transplant centers. However, no viral load test has been approved by the FDA. Therefore, many laboratories rely on laboratory-developed assays. This study evaluated the performance char...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.02558-06

    authors: Caliendo AM,Ingersoll J,Fox-Canale AM,Pargman S,Bythwood T,Hayden MK,Bremer JW,Lurain NS

    更新日期:2007-06-01 00:00:00

  • Stability of cervical specimens in SurePath medium for human papillomavirus testing with the Roche cobas 4800 assay.

    abstract::The stability of cervical specimens in SurePath preservative fluid for human papillomavirus (HPV) testing with Roche cobas 4800 was determined using a panel of 308 pooled specimens from a colposcopy referral population. The SurePath specimens appeared to be stable for up to 10 weeks at ambient temperature for HPV test...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01391-13

    authors: Gilbert L,Oates E,Ratnam S

    更新日期:2013-10-01 00:00:00

  • Diagnosis of influenza A virus infections by detection of specific immunoglobulins M, A, and G in serum.

    abstract::The value of immunoglobulin M (IgM) detection in the early diagnosis of influenza A was examined in a prospective study during an outbreak of influenza A/Philippines/2/82 (H3N2) virus infection in February and March 1986. The IgM response was investigated in sera from 64 adults with influenzalike symptoms; we found a ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.27.3.453-458.1989

    authors: Vikerfors T,Lindegren G,Grandien M,van der Logt J

    更新日期:1989-03-01 00:00:00

  • Improved detection times for Mycobacterium avium complex and Mycobacterium tuberculosis with the BACTEC radiometric system.

    abstract::A total of 2,559 routine clinical specimens were cultured for mycobacteria by using BACTEC Middlebrook 7H12 medium (BACTEC), Lowenstein-Jensen slants (LJ), and Mycobactosel selective Middlebrook 7H11 slants (M7H11). Thirty-three isolates (1.3%) of M. avium complex and 82 isolates (3.2%) of M. tuberculosis were recover...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.22.5.841-845.1985

    authors: Kirihara JM,Hillier SL,Coyle MB

    更新日期:1985-11-01 00:00:00

  • mecA gene is widely disseminated in Staphylococcus aureus population.

    abstract::Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most important causes of hospital infections worldwide. High-level resistance to methicillin is caused by the mecA gene, which encodes an alternative penicillin-binding protein, PBP 2a. To determine the clonal relationships between methicillin-susceptibl...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.40.11.3970-3975.2002

    authors: Wielders CL,Fluit AC,Brisse S,Verhoef J,Schmitz FJ

    更新日期:2002-11-01 00:00:00

  • Comparison of bacteria with and without plasmid-encoded proteins as antigens for measurement of immunoglobulin M, G, and A antibodies to Yersinia enterocolitica by enzyme-linked immunosorbent assay.

    abstract::Yersinia enterocolitica serovar O:3 bacteria with and without plasmid-encoded proteins were compared as antigens in an enzyme-linked immunosorbent assay. Good correlations between the two antigen preparations were obtained for immunoglobulin M (IgM), IgG, and IgA antibodies of patients with yersiniosis. For routine di...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.27.3.583-585.1989

    authors: Granfors K,Lahesmaa-Rantala R,Ståhlberg TH,Toivanen A

    更新日期:1989-03-01 00:00:00

  • Genotypic characterization of ureaplasma serovars from clinical isolates by pulsed-field gel electrophoresis.

    abstract::Genetic relationships within ureaplasma serovars were investigated by pulsed-field gel electrophoresis (PFGE). One hundred thirteen Ureaplasma parvum isolates and 78 Ureaplasma urealyticum isolates were different from their ATCC serovar type strains and different within the same serovars. The organisms were geographic...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.01235-11

    authors: Xiao L,Crabb DM,Moser SA,Duffy LB,Glass JI,Paralanov V,Waites KB

    更新日期:2011-09-01 00:00:00

  • Identification of Staphylococcus species of bovine origin with the API Staph-Ident system.

    abstract::The API Staph-Ident system was evaluated as a means for identifying the species of bovine strains of staphylococci routinely isolated from quarter-milk samples. The species identity of 314 of 581 (54%) isolates of staphylococci was correctly determined by this method. The API Staph-Ident system was more accurate in co...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.18.5.1212-1219.1983

    authors: Langlois BE,Harmon RJ,Akers K

    更新日期:1983-11-01 00:00:00

  • Predictors of relapse of methicillin-resistant Staphylococcus aureus bacteremia after treatment with vancomycin.

    abstract::The risk factors for relapse of methicillin-resistant Staphylococcus aureus (MRSA) bacteremia after vancomycin treatment are unknown. Diversilab typing was used to classify recurrent bacteremia as relapse or reinfection. Bacteremia for >7 days and staphylococcal cassette chromosome mec element (SCCmec) type II were in...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.05287-11

    authors: Welsh KJ,Skrobarcek KA,Abbott AN,Lewis CT,Kruzel MC,Lewis EM,Gardiner JM,Mohr JF,Armitige LY,Wanger A

    更新日期:2011-10-01 00:00:00

  • Novel selective medium for isolation of Burkholderia pseudomallei.

    abstract::Isolation of Burkholderia pseudomallei currently relies on the use of Ashdown's selective agar (ASA). We designed a new selective agar (Burkholderia pseudomallei selective agar [BPSA]) to improve recovery of the more easily inhibited strains of B. pseudomallei. B. pseudomallei, Burkholderia cepacia, and Pseudomonas ae...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/jcm.41.7.3312-3316.2003

    authors: Howard K,Inglis TJ

    更新日期:2003-07-01 00:00:00

  • Comparison and evaluation of antimicrobial susceptibility testing of enterococci performed in accordance with six national committee standardized disk diffusion procedures.

    abstract::Studies were conducted to compare and evaluate antimicrobial susceptibility test results for enterococci obtained by six national committee disk diffusion procedures. Variations in the incidence of isolates in resistance categories and errors were associated with the use of ciprofloxacin, gentamicin, nitrofurantoin, r...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.39.10.3753-3756.2001

    authors: Cotter G,Adley CC

    更新日期:2001-10-01 00:00:00

  • Serological evidence for increased human exposure to Burkholderia pseudomallei following the tsunami in southern Thailand.

    abstract::A serological study was performed to determine recent human exposure to Burkholderia pseudomallei (the cause of melioidosis) in residents of southern Thailand affected by the tsunami of 26 December 2004. The findings were suggestive of increased recent exposure in both tsunami survivors and uninjured bystanders. Survi...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.44.1.239-240.2006

    authors: Wuthiekanun V,Chierakul W,Rattanalertnavee J,Langa S,Sirodom D,Wattanawaitunechai C,Winothai W,White NJ,Day N,Peacock SJ

    更新日期:2006-01-01 00:00:00

  • Prototheca wickerhamii mimicking yeast: a cautionary tale.

    abstract::Prototheca spp. are environmental algae that may cause serious infection in the immunocompromised patient. Clinical manifestations may mimic other diseases. We present a case of fatal infection in a 78-year-old cardiac transplant recipient and discuss pitfalls in the clinical and laboratory diagnoses. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.00487-11

    authors: McMullan B,Muthiah K,Stark D,Lee L,Marriott D

    更新日期:2011-08-01 00:00:00

  • Sensitivity and specificity of rapid diagnostic tests for detection of group B streptococcal antigen in bacteremic neonates.

    abstract::Latex particle agglutination (LPA) testing for antigen to group B streptococcus (GBS) has been useful in the diagnosis of GBS sepsis in newborns. However, recent reports have demonstrated that the sensitivity of LPA assays may be as low as 27 to 54%. The purposes of the present study were to directly compare the abili...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章

    doi:10.1128/JCM.33.1.193-198.1995

    authors: Greenberg DN,Ascher DP,Yoder BA,Hensley DM,Heiman HS,Keith JF 3rd

    更新日期:1995-01-01 00:00:00