Improved detection times for Mycobacterium avium complex and Mycobacterium tuberculosis with the BACTEC radiometric system.

abstract：:Herpesviruses isolated from either domesticated or wild carnivores should be immunologically compared with known viruses of this group which could have been included in the diet of the animal before being considered to be previously undescribed herpesviruses native to the carnivore in question. ...

journal_title：Journal of clinical microbiology

authors： Porter DD,Larsen AE,Cox NA

更新日期：1975-01-01 00:00:00

abstract：:As part of an ongoing investigation into nosocomial Legionella infections at Stanford University Medical Center (SUMC), we applied the technique of restriction endonuclease analysis (REA) to determine strain differences among three species, including Legionella pneumophila, Legionella dumoffii, and Legionella micdadei...

journal_title：Journal of clinical microbiology

authors： Tompkins LS,Troup NJ,Woods T,Bibb W,McKinney RM

更新日期：1987-10-01 00:00:00

abstract：:The efficacy of antiviral treatment for chronic hepatitis C virus (HCV) infection is determined by measuring HCV RNA at specific time points throughout therapy using highly sensitive and accurate HCV RNA assays. This study compared the performances of two recently developed real-time PCR HCV RNA assays, cobas HCV for ...

journal_title：Journal of clinical microbiology

authors： Vermehren J,Stelzl E,Maasoumy B,Michel-Treil V,Berkowski C,Marins EG,Paxinos EE,Marino E,Wedemeyer H,Sarrazin C,Kessler HH

更新日期：2017-04-01 00:00:00

abstract：:Leishmania (Viannia) braziliensis causes three main types of American tegumentary leishmaniasis (ATL), localized cutaneous leishmaniasis (CL), mucosal leishmaniasis (ML), and disseminated leishmaniasis (DL). All forms are observed among individuals of Corte de Pedra, Brazil. We previously used random amplified markers...

journal_title：Journal of clinical microbiology

authors： Queiroz A,Sousa R,Heine C,Cardoso M,Guimarães LH,Machado PR,Carvalho EM,Riley LW,Wilson ME,Schriefer A

更新日期：2012-12-01 00:00:00

abstract：:Ovine progressive pneumonia virus (OPPV) is a lentivirus which causes a progressive disease in sheep. Immunodominant epitopes have been identified in the envelope gp40 glycoprotein. Synthetic peptides representing these regions are able to detect the presence of OPPV antibodies in 96% of infected sheep. ...

journal_title：Journal of clinical microbiology

authors： Kwang J,Torres JV

更新日期：1994-07-01 00:00:00

abstract：:We demonstrated that sodium gluconate was the factor causing false-positive galactomannan (GM) antigenemia of Plasma-Lyte hydration solution. Infusion of sodium gluconate-containing solution but not gluconate-free Plasma-Lyte resulted in positive serum GM antigenemia. Serum GM concentrations also correlated with the v...

journal_title：Journal of clinical microbiology

authors： Petraitiene R,Petraitis V,Witt JR 3rd,Durkin MM,Bacher JD,Wheat LJ,Walsh TJ

更新日期：2011-12-01 00:00:00

abstract：:We describe two cases of eumycetoma in the legs. The infections could not be adequately diagnosed by classical mycology, but the causative agents were successfully identified as Madurella mycetomatis by species-specific PCR and DNA sequencing. ...

journal_title：Journal of clinical microbiology

authors： Ahmed AO,Desplaces N,Leonard P,Goldstein F,De Hoog S,Verbrugh H,van Belkum A

更新日期：2003-12-01 00:00:00

abstract：:In Mycobacterium smegmatis and a limited number of Mycobacterium tuberculosis strains, the involvement of alterations of the 16S rRNA gene (rrs) in resistance to kanamycin has been shown. To investigate the extent to which mutations in a specific region of the rrs gene and the kanamycin-resistant phenotype in clinical...

journal_title：Journal of clinical microbiology

authors： Suzuki Y,Katsukawa C,Tamaru A,Abe C,Makino M,Mizuguchi Y,Taniguchi H

更新日期：1998-05-01 00:00:00

abstract：:The new colorimetric Vitek 2 YST card was evaluated for identification of yeasts (136 strains) with respect to the influence of different source media. The Vitek 2 YST card achieved satisfactory results for all yeast species tested, with the exception of Candida guilliermondii, Candida norvegensis, Candida parapsilosi...

journal_title：Journal of clinical microbiology

authors： Valenza G,Strasen J,Schäfer F,Frosch M,Kurzai O,Abele-Horn M

更新日期：2008-11-01 00:00:00

abstract：:A collection of 147 isolates of Burkholderia pseudomallei, B. mallei, and B. thailandensis was characterized by multilocus sequence typing (MLST). The 128 isolates of B. pseudomallei, the causative agent of melioidosis, were obtained from diverse geographic locations, from humans and animals with disease, and from the...

journal_title：Journal of clinical microbiology

authors： Godoy D,Randle G,Simpson AJ,Aanensen DM,Pitt TL,Kinoshita R,Spratt BG

更新日期：2003-05-01 00:00:00

abstract：:Specimens submitted for diagnosis of herpes simplex virus infections were inoculated into shell vials and conventional culture tubes. Inoculated culture tubes were incubated with rolling at 96 rpm. Immunoperoxidase (IP) staining and cytopathic effects (CPE) were used to detect positive cultures. At 24 h, 42 (53%) of t...

journal_title：Journal of clinical microbiology

authors： Hughes JH,Hamparian VV,Mavromoustakis CT

更新日期：1989-12-01 00:00:00

abstract：:A novel PCR restriction analysis method using the RNA polymerase beta-subunit- coding gene (rpoB) was employed to both detect and identify Helicobacter pylori in biopsy specimens and culture isolates. The rpoB DNAs (458 bp) were specifically amplified by PCR with the Helicobacter-specific primers (HF and HR). Based on...

journal_title：Journal of clinical microbiology

authors： Lim CY,Lee KH,Cho MJ,Chang MW,Kim SY,Myong NH,Lee WK,Rhee KH,Kook YH

更新日期：2003-07-01 00:00:00

abstract：:Typing and in vitro cultivation of rotavirus-positive human stool samples stored unintentionally at ambient tropical temperatures for 2 1/2 months showed that rotavirus is stable and may still be infectious in vitro. This indicates that stool specimen collection for rotavirus studies can be performed in areas and sett...

journal_title：Journal of clinical microbiology

authors： Fischer TK,Steinsland H,Valentiner-Branth P

更新日期：2002-12-01 00:00:00

abstract：:The genetic variation within a bacterial clone (known as Shigella sonnei but in effect a clone of Escherichia coli) was examined by studying 46 clinical isolates that were epidemiologically unassociated and isolated from patients in different countries over a period of 41 years (1950 to 1991). Restriction fragment len...

journal_title：Journal of clinical microbiology

authors： Karaolis DK,Lan R,Reeves PR

更新日期：1994-03-01 00:00:00

abstract：:CDC group M-5 is a rod-shaped, gram-negative, nonmotile bacterium associated with dog bite wounds. DNA-DNA relatedness and biochemical and growth characteristics were studied for 54 strains from the collection at the Centers for Disease Control and Prevention. One typical M-5 strain, 8142, was further studied by 16S r...

journal_title：Journal of clinical microbiology

authors： Andersen BM,Steigerwalt AG,O'Connor SP,Hollis DG,Weyant RS,Weaver RE,Brenner DJ

更新日期：1993-09-01 00:00:00

abstract：:Colony phenotype and genetic similarity were assessed within and between groups of commensal and pathogenic strains of Candida albicans collected from the oral cavities of individuals in a single geographical locale. Thirty-eight percent of pathogenic isolates contained predominant or minor variant colony morphologies...

journal_title：Journal of clinical microbiology

authors： Hellstein J,Vawter-Hugart H,Fotos P,Schmid J,Soll DR

更新日期：1993-12-01 00:00:00

abstract：:The volume of blood sampled for blood culture determines its sensitivity. We measured low mean blood volumes in submitted aerobic (8.38 ± 3.88 ml) and anaerobic (7.16 ± 3.83 ml) blood culture bottles. Educational seminars were held for phlebotomy teams, and renewed measurements thereafter revealed significantly higher...

journal_title：Journal of clinical microbiology

authors： van Ingen J,Hilt N,Bosboom R

更新日期：2013-03-01 00:00:00

abstract：:MARC-145 cell line is commonly used to isolate porcine reproductive and respiratory syndrome virus (PRRSV) for diagnostics, research and vaccine production, but it yields frustratingly low success rates of virus isolation (VI). ZMAC cell line, derived from porcine alveolar macrophages, has become available but its uti...

journal_title：Journal of clinical microbiology

authors： Yim-Im W,Huang H,Park J,Wang C,Calzada G,Gauger P,Harmon K,Main R,Zhang J

更新日期：2020-12-02 00:00:00

abstract：:A procedure was developed for characterization of Chlamydia trachomatis strains by using restriction endonuclease analysis of amplified genes of the major outer membrane protein (MOMP). Reference strains of the 15 serovars (A through K and L1 through L3) and clinical isolates were tested. The nucleotide sequences of t...

journal_title：Journal of clinical microbiology

authors： Rodriguez P,Vekris A,de Barbeyrac B,Dutilh B,Bonnet J,Bebear C

更新日期：1991-06-01 00:00:00

abstract：:The diagnosis of Clostridium difficile infection continues to be a challenge for many clinical microbiology laboratories. A new lateral flow assay, the C.Diff Quik Chek Complete assay, which tests for the presence of both glutamate dehydrogenase (GDH) and C. difficile toxins A and B, was evaluated for its ability to d...

journal_title：Journal of clinical microbiology

authors： Sharp SE,Ruden LO,Pohl JC,Hatcher PA,Jayne LM,Ivie WM

更新日期：2010-06-01 00:00:00

abstract：:Serratia marcescens is a well-known cause of nosocomial infections and outbreaks, particularly in critically ill neonates and immunocompromised patients. Numerous methods have been proposed for typing. We used pulsed-field gel electrophoresis (PFGE) typing to analyze an outbreak in a neonatal intensive care unit (NICU...

journal_title：Journal of clinical microbiology

authors： Miranda G,Kelly C,Solorzano F,Leanos B,Coria R,Patterson JE

更新日期：1996-12-01 00:00:00

abstract：:Early and accurate diagnosis is essential for optimal therapeutic outcomes in patients infected with HIV. Currently, none of the commercially available fourth-generation assays differentiate HIV-1 and HIV-2 antibodies (Ab) or the HIV-1 p24 antigen (Ag). The aim of this study was to evaluate the performance of a novel ...

journal_title：Journal of clinical microbiology

authors： Salmona M,Delarue S,Delaugerre C,Simon F,Maylin S

更新日期：2014-01-01 00:00:00

abstract：:A search has been made for corynebacterial phospholipase D, "ovis toxin," a sphingomyelinase (phosphatidylcholine phosphohydrolase, EC 3.1.4.4), among a wide variety of corynebacteria. Phospholipase D activity has been found in strains exhibiting the biochemical properties characteristic of Corynebacterium pseudotuber...

journal_title：Journal of clinical microbiology

authors： Barksdale L,Linder R,Sulea IT,Pollice M

更新日期：1981-02-01 00:00:00

abstract：:A quantitative PCR assay for the detection of human herpesvirus 6 (HHV-6) (variants A and B) and HHV-7 DNAs in clinical samples was developed. The assay uses a nonhomologous internal standard (IS) for each virus that is coamplified with the wild-type target sequence in the same vial and with the same pair of primers. ...

journal_title：Journal of clinical microbiology

authors： Secchiero P,Zella D,Crowley RW,Gallo RC,Lusso P

更新日期：1995-08-01 00:00:00

abstract：:A pseudooutbreak of cryptococcal meningitis was caused by the use of contaminated albumin solution used in the preparation of Papanicolaou-stained slides of cerebrospinal fluid. Organisms were seen in cytocentrifuge preparations, but not in India ink preparations of cerebrospinal fluid specimens. Cryptococcal antigen ...

journal_title：Journal of clinical microbiology

authors： Hopfer RL,Katz RL,Fainstein V

更新日期：1982-06-01 00:00:00

abstract：:The first outbreak of Candida haemulonii fungemia is described. The seven isolates from the blood of four neonates were identified by DNA sequencing of the ribosomal DNA. They were all resistant to amphotericin B, fluconazole, and itraconazole. This report highlights the emergence of C. haemulonii as an opportunistic ...

journal_title：Journal of clinical microbiology

authors： Khan ZU,Al-Sweih NA,Ahmad S,Al-Kazemi N,Khan S,Joseph L,Chandy R

更新日期：2007-06-01 00:00:00

abstract：:The algorithm for a new identification system was designed on the basis of colony color and morphology on CHROMagar Orientation medium in conjunction with simple biochemical tests such as indole (IND), lysine decarboxylase (LDC), and ornithine decarboxylase (ODC) utilization tests with gram-negative bacilli isolated f...

journal_title：Journal of clinical microbiology

authors： Ohkusu K

更新日期：2000-12-01 00:00:00

abstract：:Mycoplasma hyopneumoniae is an important cause of pneumonia in pigs around the world, but confirming its presence in (or absence from) pigs can be difficult. Culture for diagnosis is impractical, and seroconversion is often delayed after natural infection, limiting the use of serology. Numerous PCR assays for the dete...

journal_title：Journal of clinical microbiology

authors： Strait EL,Madsen ML,Minion FC,Christopher-Hennings J,Dammen M,Jones KR,Thacker EL

更新日期：2008-08-01 00:00:00

abstract：:The newly emerged Middle East respiratory syndrome coronavirus (MERS-CoV) has infected at least 1,082 people, including 439 fatalities. So far, no empirical virus isolation study has been done to elucidate infectious virus secretion or serotype variability. Here, we used 51 respiratory samples from 32 patients with co...

journal_title：Journal of clinical microbiology

authors： Muth D,Corman VM,Meyer B,Assiri A,Al-Masri M,Farah M,Steinhagen K,Lattwein E,Al-Tawfiq JA,Albarrak A,Müller MA,Drosten C,Memish ZA

更新日期：2015-09-01 00:00:00

abstract：:Detection of the source of Clostridium difficile strains is of importance for the control of the nosocomial spread of this microorganism. For this purpose, vaginal and rectal swabs from 183 mothers, duplicate fecal samples (taken on days 1 and 4 after birth) from 183 neonates, and 94 environmental samples were culture...

journal_title：Journal of clinical microbiology

authors： Martirosian G,Kuipers S,Verbrugh H,van Belkum A,Meisel-Mikolajczyk F

更新日期：1995-08-01 00:00:00