Comparison of ZMAC and MARC-145 Cell Lines for Improving Porcine Reproductive and Respiratory Syndrome Virus Isolation from Clinical Samples.

abstract：:Establishment of latently infected cell lines from primary effusion lymphomas (PEL) presently is the most efficient system for the propagation of clinical strains of human herpesvirus 8 (HHV-8) in culture. Here we describe a new approach to culture productively replicating HHV-8 from patient samples. A BJAB-derived B-...

journal_title：Journal of clinical microbiology

authors： Gasperini P,Barbierato M,Martinelli C,Rigotti P,Marchini F,Masserizzi G,Leoncini F,Chieco-Bianchi L,Schulz TF,Calabrò ML

更新日期：2005-06-01 00:00:00

abstract：:Because strains of Staphylococcus aureus that are resistant to penicillinase-resistant penicillins may be difficult to detect in the clinical laboratory, a variety of changes in methodology have been suggested to increase their detection. In 1984, the West Los Angeles Veterans Administration Medical Center experienced...

journal_title：Journal of clinical microbiology

authors： Mulligan ME,Citron DM,Kwok RY,Wheelock JP,Farrohi FK,Hindler JA,Johnston L

更新日期：1987-05-01 00:00:00

abstract：:A multiresistant strain of Pseudomonas aeruginosa is widespread among cystic fibrosis (CF) patients attending clinics in Liverpool, United Kingdom. Suppression subtractive hybridization was used to identify sequences present in the Liverpool CF epidemic strain but absent from strain PAO1. Using dot blot and PCR amplif...

journal_title：Journal of clinical microbiology

authors： Parsons YN,Panagea S,Smart CH,Walshaw MJ,Hart CA,Winstanley C

更新日期：2002-12-01 00:00:00

abstract：:Characterization of 102 Haemophilus strains by the Minitek differentiation system (BBL Microbiology Systems, Cockeysville, Md.) was compared with that by the conventional method. The results obtained by the two methods were in good agreement (P less than 0.001; test of independence). Therefore, this system was found t...

journal_title：Journal of clinical microbiology

authors： Kawakami Y,Okimura Y,Kanai M

更新日期：1981-11-01 00:00:00

abstract：:We standardized and assessed the performance of an in-house microtiter assay for determining the susceptibilities of Mycobacterium tuberculosis clinical isolates to isoniazid based on mycobacteriophage amplification technology. Seventy isolates (43 resistant and 27 sensitive according to the BACTEC 460 radiometric met...

journal_title：Journal of clinical microbiology

authors： Galí N,Domínguez J,Blanco S,Prat C,Alcaide F,Coll P,Ausina V

更新日期：2006-01-01 00:00:00

abstract：:Sepsis is a major health problem in newborns and children. Early detection of pathogens allows initiation of appropriate antimicrobial therapy that strongly correlates with positive outcomes. Multiplex PCR has the potential to rapidly identify bloodstream infections, compensating for the loss of blood culture sensitiv...

journal_title：Journal of clinical microbiology

authors： Lucignano B,Ranno S,Liesenfeld O,Pizzorno B,Putignani L,Bernaschi P,Menichella D

更新日期：2011-06-01 00:00:00

abstract：:The development and evaluation of a new selective medium (colistin-oxolinic acid-blood agar) for streptococci is described. Streptococci of medical and veterinary importance grew well on the medium. Gram-negative organisms, staphylococci. Bacillus spp., and coryneforms are all inhibited. It was concluded that the medi...

journal_title：Journal of clinical microbiology

authors： Petts DN

更新日期：1984-01-01 00:00:00

abstract：:We present a duplex, real-time PCR assay for detection of Klebsiella pneumoniae carbapenemase (blaKPC) and New Delhi metallo-β-lactamase (blaNDM) genes. Accuracy was assessed with 158 Gram-negative bacillary isolates, including 134 carbapenemase producers. The assay had 100% sensitivity and specificity compared with r...

journal_title：Journal of clinical microbiology

authors： Cunningham SA,Noorie T,Meunier D,Woodford N,Patel R

更新日期：2013-04-01 00:00:00

abstract：:Detection and genotyping of Chlamydia trachomatis were optimized by using a polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis performed directly with crude cells of cervical scrapes. Different PCR pretreatment methods were evaluated on samples which were positive for C. trach...

journal_title：Journal of clinical microbiology

authors： Lan J,Walboomers JM,Roosendaal R,van Doornum GJ,MacLaren DM,Meijer CJ,van den Brule AJ

更新日期：1993-05-01 00:00:00

abstract：:VP1 sequences were determined for poliovirus type 1 isolates obtained over a 189-day period from a poliomyelitis patient with common variable immunodeficiency syndrome (a defect in antibody formation). The isolate from the first sample, taken 11 days after onset of paralysis, contained two poliovirus populations, diff...

journal_title：Journal of clinical microbiology

authors： Kew OM,Sutter RW,Nottay BK,McDonough MJ,Prevots DR,Quick L,Pallansch MA

更新日期：1998-10-01 00:00:00

abstract：:Hormographiella aspergillata, a filamentous basidiomycete, has rarely been involved in human infections. We describe 2 febrile neutropenic patients who developed a severe pulmonary infection due to H. aspergillata while receiving empirical caspofungin therapy for presumed fungal pneumonia. After introduction of liposo...

journal_title：Journal of clinical microbiology

authors： Suarez F,Olivier G,Garcia-Hermoso D,Randriamalala E,Ghez D,Bruneau J,Kauffmann-Lacroix C,Bougnoux ME,Lortholary O

更新日期：2011-01-01 00:00:00

abstract：:Monoclonal antibodies to K88ac and K88ab were used in enzyme-linked immunosorbent assays on Escherichia coli cultures known to produce K88 pili. A total of 415 K88-positive E. coli isolates from nine states were all found to be the K88ac variant. The cultures tested were isolated during the years 1976 to 1985. ...

journal_title：Journal of clinical microbiology

authors： Westerman RB,Mills KW,Phillips RM,Fortner GW,Greenwood JM

更新日期：1988-01-01 00:00:00

abstract：:Monkey pox virus was mechanically disrupted by low temperature and high pressure into soluble and insoluble fractions. Soluble fractions elicited virus-neutralizing antibodies (1:20 to 1:160) in rabbits, whereas the insoluble (in saline) fractions did not (less than 1:5). No infectious virus was detected after the dis...

journal_title：Journal of clinical microbiology

authors： Olsen RG,Blakeslee JR,Mathes L,Nakano JH

更新日期：1977-07-01 00:00:00

abstract：:The diagnosis of brucellosis in cattle and small ruminants requires the use of more than one serological test. The complement fixation test (CFT), the rose bengal test (RBT), and the serum agglutination test (SAT) are among the most useful tests for routine diagnosis. The microagglutination test (MAT) was developed as...

journal_title：Journal of clinical microbiology

authors： Baum M,Zamir O,Bergman-Rios R,Katz E,Beider Z,Cohen A,Banai M

更新日期：1995-08-01 00:00:00

abstract：:Humoral immune response to intestinal Rhodococcus (Corynebacterium) equi in horses was studied by enzyme-linked immunosorbent assay. Anti-R. equi immunoglobulin M (IgM), IgG, and IgA antibodies were demonstrated in the healthy horse population. Adult horse levels of anti-R. equi IgM and IgG antibodies were reached by ...

journal_title：Journal of clinical microbiology

authors： Takai S,Kawazu S,Tsubaki S

更新日期：1986-05-01 00:00:00

abstract：:Hafnia alvei strains which possess the attachment-effacement gene (eaeA) may have clinical importance as new diarrhea-causing pathogens and should therefore be differentiated from other H. alvei strains. We characterized diarrheal H. alvei strains, which were positive in the PCR test for the eaeA gene, using biochemic...

journal_title：Journal of clinical microbiology

authors： Ridell J,Siitonen A,Paulin L,Lindroos O,Korkeala H,Albert MJ

更新日期：1995-09-01 00:00:00

abstract：:Severe early childhood caries (ECC), while strongly associated with Streptococcus mutans using selective detection (culture, PCR), has also been associated with a widely diverse microbiota using molecular cloning approaches. The aim of this study was to evaluate the microbiota of severe ECC using anaerobic culture. Th...

journal_title：Journal of clinical microbiology

authors： Tanner AC,Mathney JM,Kent RL,Chalmers NI,Hughes CV,Loo CY,Pradhan N,Kanasi E,Hwang J,Dahlan MA,Papadopolou E,Dewhirst FE

更新日期：2011-04-01 00:00:00

abstract：:High-frequency switching and strain variability at the site of infection was assessed in 11 patients with acute Candida albicans vaginitis. By cloning cells directly from the site of infection, it was demonstrated that 4 of the 11 isolates contained multiple-switch phenotypes at the site of infection and that 9 of the...

journal_title：Journal of clinical microbiology

authors： Soll DR,Langtimm CJ,McDowell J,Hicks J,Galask R

更新日期：1987-09-01 00:00:00

abstract：:A total of 563 specimens (234 dermal and 329 genital swabs) from patients suspected of having herpes simplex virus (HSV) infections were processed using two different extraction methods (the MagNA Pure LC system and the swab extraction tube system [SETS]); HSV DNA was amplified by LightCycler PCR. HSV DNA was detected...

journal_title：Journal of clinical microbiology

authors： Issa NC,Espy MJ,Uhl JR,Harmsen WS,Mandrekar JN,Gullerud RE,Davis MD,Smith TF

更新日期：2005-03-01 00:00:00

abstract：:The way in which the extensive use of highly active antiretroviral therapy (HAART) has influenced the incidence of visceral leishmaniasis (VL) among human immunodeficiency type 1 (HIV-1)-infected patients is not yet understood. The present study assessed whether the incidence of symptomatic VL in HIV-infected patients...

journal_title：Journal of clinical microbiology

authors： de La Rosa R,Pineda JA,Delgado J,Macías J,Morillas F,Mira JA,Sánchez-Quijano A,Leal M,Lissen E

更新日期：2002-03-01 00:00:00

abstract：:Recent studies have resulted in the proposal of a new species, Aeromonas schubertii (mannitol, sucrose, and indole negative), formerly termed Enteric Group 501, on the basis of the study of seven strains isolated from the southeastern and southwestern United States and Puerto Rico. We have isolated two phenotypically ...

journal_title：Journal of clinical microbiology

authors： Carnahan AM,Marii MA,Fanning GR,Pass MA,Joseph SW

更新日期：1989-08-01 00:00:00

abstract：:Seventy-eight blood cultures with a Gram stain result of Gram-positive cocci in pairs and/or chains were evaluated with the Nanosphere Verigene Gram-positive blood culture (BC-GP) assay. The overall concordance of the assay with culture was 89.7% (70/78 cultures), allowing for the development of a targeted treatment a...

journal_title：Journal of clinical microbiology

authors： Alby K,Daniels LM,Weber DJ,Miller MB

更新日期：2013-11-01 00:00:00

abstract：:Reverse transcription-PCR (RT-PCR) is a sensitive method for detection of RNA virus nucleic acid sequences in clinical respiratory specimens. Previous studies have focused on RT-PCR for a single virus, but this approach is limited by the inability to establish a specific etiology when the RT-PCR result is negative and...

journal_title：Journal of clinical microbiology

authors： Gilbert LL,Dakhama A,Bone BM,Thomas EE,Hegele RG

更新日期：1996-01-01 00:00:00

abstract：:During the course of a molecular epidemiology study of mechanisms of antibiotic resistance in the area served by our hospital (516,000 inhabitants), we isolated the gene encoding CTX-M-14 beta-lactamase. Thirty clinical strains (27 Escherichia coli and 3 Klebsiella pneumoniae isolates) with a phenotype of extended-spe...

journal_title：Journal of clinical microbiology

authors： Bou G,Cartelle M,Tomas M,Canle D,Molina F,Moure R,Eiros JM,Guerrero A

更新日期：2002-11-01 00:00:00

abstract：:Alpha-toxin is a major Staphylococcus aureus virulence factor. This study evaluated potential relationships between in vitro alpha-toxin expression of S. aureus bloodstream isolates, anti-alpha-toxin antibody in serum of patients with S. aureus bacteremia (SAB), and clinical outcomes in 100 hemodialysis and 100 postsu...

journal_title：Journal of clinical microbiology

authors： Sharma-Kuinkel BK,Wu Y,Tabor DE,Mok H,Sellman BR,Jenkins A,Yu L,Jafri HS,Rude TH,Ruffin F,Schell WA,Park LP,Yan Q,Thaden JT,Messina JA,Fowler VG Jr,Esser MT

更新日期：2015-01-01 00:00:00

abstract：:The production and characterization of five monoclonal antibodies to Mycobacterium tuberculosis are described. Specificity of the monoclonal antibodies was tested against other mycobacterial species by an enzyme-linked immunosorbent assay and immunoblots. HGT 3a, an immunoglobulin M (IgM) antibody, recognizes a molecu...

journal_title：Journal of clinical microbiology

authors： Kadival GV,Chaparas SD

更新日期：1987-01-01 00:00:00

abstract：:The increased incidence of nosocomial Legionnaires' disease in two hospitals prompted investigation of possible environmental sources. In the search for an effective DNA-typing technique for use in hospital epidemiology, the performance and convenience of three methods-SfiI macrorestriction analysis (MRA), amplified f...

journal_title：Journal of clinical microbiology

authors： Jonas D,Meyer HG,Matthes P,Hartung D,Jahn B,Daschner FD,Jansen B

更新日期：2000-06-01 00:00:00

abstract：:In this study, we determined the sequence of the EcoRI-PstI fragment of the plasmid pCVD432, also termed the enteroaggregative Escherichia coli (EAggEC) probe. A primer pair complementary to this probe was designed for PCR amplification of a 630-bp region. Comparison of the analysis of the EAggEC probe sequence with t...

journal_title：Journal of clinical microbiology

authors： Schmidt H,Knop C,Franke S,Aleksic S,Heesemann J,Karch H

更新日期：1995-03-01 00:00:00

abstract：:Bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV) are orbiviruses that infect both livestock and wild ruminants. Antigenic cross-reactivity between BTV and EHDV often results in serologic misdiagnosis. Competitive enzyme-linked immunosorbent assays (c-ELISAs) show increased sensitivity and specific...

journal_title：Journal of clinical microbiology

authors： Mecham JO,Wilson WC

更新日期：2004-02-01 00:00:00

abstract：:Mumps-specific antibody levels before and after vaccination with live mumps vaccines were determined by enzyme-linked immunosorbent assay (ELISA) and neutralization tests. A correlation was found between neutralization titers and optical density in ELISA. However, postvaccination sera from some vaccinees who failed to...

journal_title：Journal of clinical microbiology

authors： Sakata H,Hishiyama M,Sugiura A

更新日期：1984-01-01 00:00:00