Evaluation of new colorimetric vitek 2 yeast identification card by use of different source media.

abstract：:Droplet digital PCR (ddPCR) is an emulsion PCR process that performs absolute quantitation of nucleic acids. We developed a ddPCR assay for Chlamydia trachomatis infections and found it to be accurate and precise. Using PCR mixtures containing plasmids engineered to include the PCR target sequences, we were able to qu...

journal_title：Journal of clinical microbiology

authors： Roberts CH,Last A,Molina-Gonzalez S,Cassama E,Butcher R,Nabicassa M,McCarthy E,Burr SE,Mabey DC,Bailey RL,Holland MJ

更新日期：2013-07-01 00:00:00

abstract：:Monoclonal antibodies (MAb) were produced against the specific Bordetella pertussis antigen pertussis toxin (PT). In preliminary studies, one MAb (IB12) was selected and used in an enzyme-linked dot blot immunoassay to evaluate the ability of the method to detect known amounts of PT in control experiments and to test ...

journal_title：Journal of clinical microbiology

authors： Friedman RL,Paulaitis S,McMillan JW

更新日期：1989-11-01 00:00:00

abstract：:We report in this work a highly sensitive and nonradioactive PCR method for the detection of the four species of parasite causing human malaria. Plasmodium-specific primers corresponding to the small-subunit rRNA genes of the malaria parasite were used, and a 291-bp fragment was amplified. Our results showed a high sp...

journal_title：Journal of clinical microbiology

authors： Ciceron L,Jaureguiberry G,Gay F,Danis M

更新日期：1999-01-01 00:00:00

abstract：:This report presents the case of a patient with a massive pyelonephritis and a urosepsis caused by Streptococcus pneumoniae. This case is unusual as the focus was distant from the respiratory tract, the usual primary site of infection caused by this organism. No other primary site of infection was documented. ...

journal_title：Journal of clinical microbiology

authors： Dufke S,Kunze-Kronawitter H,Schubert S

更新日期：2004-09-01 00:00:00

abstract：:A modified Elek test and an immuno-halo test were developed for detection of the thermostable direct hemolysin produced by Kanagawa phenomenon-positive strains of Vibrio parahaemolyticus. The sensitivity of the modified Elek test was almost the same as that of the Kanagawa phenomenon on Wagatsuma medium, and the resul...

journal_title：Journal of clinical microbiology

authors： Honda T,Chearskul S,Takeda Y,Miwatani T

更新日期：1980-06-01 00:00:00

abstract：:Stool specimens from 124 international travelers with acute diarrhea were tested for the presence of enteropathogens. Noroviruses (NoVs) were the second most commonly identified enteric pathogen in diarrheal stool samples (21/124, 17%), exceeded only by enterotoxigenic Escherichia coli (50/106, 47%). This study indica...

journal_title：Journal of clinical microbiology

authors： Ko G,Garcia C,Jiang ZD,Okhuysen PC,Belkind-Gerson J,Glass RI,DuPont HL

更新日期：2005-12-01 00:00:00

abstract：:We have developed and validated a nested real-time PCR (NRT-PCR) for the genotyping of Chlamydia trachomatis and used it specifically for the typing of either eight genovars from D to K or three genovars of lymphogranuloma venereum (LGV). The 11 probes used in the NRT-PCR correctly identified the DNA from D to K and L...

journal_title：Journal of clinical microbiology

authors： Jalal H,Stephen H,Alexander S,Carne C,Sonnex C

更新日期：2007-08-01 00:00:00

abstract：:Cultures of Sporothrix schenckii were serologically tested by the exoantigen immunodiffusion technique of Kaufman and Standard (L. Kaufman and P.G. Standard, J. Clin. Microbiol. 8:42-45, 1978). This rapid and sensitive technique permitted the identification of 10 isolates of S. schenckii within 3 days. The production ...

journal_title：Journal of clinical microbiology

authors： Polonelli L,Morace G

更新日期：1982-03-01 00:00:00

abstract：:We report a case of Coccidioides thyroiditis in an HIV-infected patient with a history of recent Coccidioides pneumonia but with negative Coccidioides serology determined by enzyme immunoassay at presentation. Diagnosis of Coccidioides thyroiditis was made based on histopathologic examination and culture of thyroid ab...

journal_title：Journal of clinical microbiology

authors： Jinno S,Chang S,Jacobs MR

更新日期：2012-07-01 00:00:00

abstract：:Current nucleic acid amplification methods to detect Mycobacterium tuberculosis are complex, labor-intensive, and technically challenging. We developed and performed the first analysis of the Cepheid Gene Xpert System's MTB/RIF assay, an integrated hands-free sputum-processing and real-time PCR system with rapid on-de...

journal_title：Journal of clinical microbiology

authors： Helb D,Jones M,Story E,Boehme C,Wallace E,Ho K,Kop J,Owens MR,Rodgers R,Banada P,Safi H,Blakemore R,Lan NT,Jones-López EC,Levi M,Burday M,Ayakaka I,Mugerwa RD,McMillan B,Winn-Deen E,Christel L,Dailey P,Perkins

更新日期：2010-01-01 00:00:00

abstract：:Although two phenotypes of the opportunistic pathogen Propionibacterium acnes (types I and II) have been described, epidemiological investigations of their roles in different infections have not been widely reported. Using immunofluorescence microscopy with monoclonal antibodies (MAbs) QUBPa1 and QUBPa2, specific for ...

journal_title：Journal of clinical microbiology

authors： McDowell A,Valanne S,Ramage G,Tunney MM,Glenn JV,McLorinan GC,Bhatia A,Maisonneuve JF,Lodes M,Persing DH,Patrick S

更新日期：2005-01-01 00:00:00

abstract：:We report a novel IS711 chromosomal location that is specific for the Brucella genotype ST27 previously associated with Pacific marine mammals and human zoonotic infection in New Zealand and Peru. Our data support the previous observation that this peculiar genotype is distinct from those commonly isolated from the At...

journal_title：Journal of clinical microbiology

authors： Cloeckaert A,Bernardet N,Koylass MS,Whatmore AM,Zygmunt MS

更新日期：2011-11-01 00:00:00

abstract：:The Light Cycler technique combines rapid in vitro amplification of DNA in glass capillaries with real-time species determination and quantification of DNA load. We have established a quantitative PCR protocol for two clinically important pathogens, Candida albicans and Aspergillus fumigatus. The sensitivity of the as...

journal_title：Journal of clinical microbiology

authors： Loeffler J,Henke N,Hebart H,Schmidt D,Hagmeyer L,Schumacher U,Einsele H

更新日期：2000-02-01 00:00:00

abstract：:The use of rapid tests for human immunodeficiency virus (HIV) has become standard in HIV testing algorithms employed in resource-limited settings. We report an extensive HIV rapid test validation study conducted among Ugandan blood bank donors at low risk for HIV infection. The operational characteristics of four read...

journal_title：Journal of clinical microbiology

authors： Eller LA,Eller MA,Ouma BJ,Kataaha P,Bagaya BS,Olemukan RL,Erima S,Kawala L,de Souza MS,Kibuuka H,Wabwire-Mangen F,Peel SA,O'Connell RJ,Robb ML,Michael NL

更新日期：2007-10-01 00:00:00

abstract：:Sequence-based typing (SBT), analogous to multilocus sequence typing (MLST), is the current "gold standard" typing method for investigation of legionellosis outbreaks caused by Legionella pneumophila However, as common sequence types (STs) cause many infections, some investigations remain unresolved. In this study, va...

journal_title：Journal of clinical microbiology

authors： David S,Mentasti M,Tewolde R,Aslett M,Harris SR,Afshar B,Underwood A,Fry NK,Parkhill J,Harrison TG

更新日期：2016-08-01 00:00:00

abstract：:The increased prevalence and the severity of oropharyngeal candidiasis in human immunodeficiency virus (HIV)-positive patients are attributed exclusively to the virus-induced immune deficiency of the host. The present study was aimed at answering the question of whether Candida albicans secretory proteinase, a putativ...

journal_title：Journal of clinical microbiology

authors： Ollert MW,Wende C,Görlich M,McMullan-Vogel CG,Borg-von Zepelin M,Vogel CW,Korting HC

更新日期：1995-10-01 00:00:00

abstract：:A modified oxidase reagent, 1% tetramethyl-p-phenylenediamine in dimethyl sulfoxide, proved superior to the routinely used 1% aqueous tetramethyl-p-phenylenediamine dihydrochloride in detecting weakly oxidase-positive gram-negative bacteria after 24 h of growth on agar media (40 of 40 positive versus 22 of 40 positive...

journal_title：Journal of clinical microbiology

authors： Tarrand JJ,Gröschel DH

更新日期：1982-10-01 00:00:00

abstract：:Patients with infection from bacteremic Bartonella spp., tested using Bartonella Alphaproteobacteria growth medium (BAPGM), were retrospectively categorized into one of two groups that included those whose blood was collected once (group 1; n = 55) or three times (group 2; n = 36) within a 1-week period. Overall, 19 p...

journal_title：Journal of clinical microbiology

authors： Pultorak EL,Maggi RG,Mascarelli PE,Breitschwerdt EB

更新日期：2013-06-01 00:00:00

abstract：:Large hospital-based clinical laboratories must be prepared to rapidly investigate potential infectious disease outbreaks. To challenge the ability of our molecular diagnostics laboratory to use whole-genome sequencing in a potential outbreak scenario and identify impediments to these efforts, we studied 84 invasive s...

journal_title：Journal of clinical microbiology

authors： Olsen RJ,Fittipaldi N,Kachroo P,Sanson MA,Long SW,Como-Sabetti KJ,Valson C,Cantu C,Lynfield R,Van Beneden C,Beres SB,Musser JM

更新日期：2014-12-01 00:00:00

abstract：:We examined clinical (pulmonary cryptococcosis and cryptococcal meningitis) and environmental (pigeon excreta) isolates of Cryptococcus neoformans var. neoformans (serotype A) in the southern Japanese prefecture of Nagasaki. The random amplified polymorphic DNA profiles obtained by using three primers revealed six pat...

journal_title：Journal of clinical microbiology

authors： Yamamoto Y,Kohno S,Koga H,Kakeya H,Tomono K,Kaku M,Yamazaki T,Arisawa M,Hara K

更新日期：1995-12-01 00:00:00

abstract：:Although TT virus (TTV) was isolated from a cryptogenic posttransfusion hepatitis patient, its pathogenic role remains unclear. It has been reported that the majority of the healthy population is infected with TTV. To elucidate the differences between TTV infection in patients with liver diseases and TTV infection in ...

journal_title：Journal of clinical microbiology

authors： Kato T,Mizokami M,Mukaide M,Orito E,Ohno T,Nakano T,Tanaka Y,Kato H,Sugauchi F,Ueda R,Hirashima N,Shimamatsu K,Kage M,Kojiro M

更新日期：2000-01-01 00:00:00

abstract：:The coagulation of Limulus amebocyte lysate (LAL) can be activated through two pathways, one initiated by endotoxin and the other by beta-glucans. The two pathways join at the step of activation of the proclotting enzyme. We report here that the endotoxin-activated pathway can be differentially inhibited by two method...

journal_title：Journal of clinical microbiology

authors： Zhang GH,Baek L,Buchardt O,Koch C

更新日期：1994-06-01 00:00:00

abstract：:We retrospectively compared the utility of a fungal isolation device (Isolator) versus conventional techniques for recovering fungal organisms from blood cultures obtained from neutropenic cancer patients. Positive cultures were deemed true pathogens, possible pathogens, or contaminants according to laboratory and cli...

journal_title：Journal of clinical microbiology

authors： Creger RJ,Weeman KE,Jacobs MR,Morrissey A,Parker P,Fox RM,Lazarus HM

更新日期：1998-01-01 00:00:00

abstract：:Fifty-six Pasteurella multocida strains (40 P. multocida subsp. septica and 16 P. multocida subsp. multocida strains) isolated from the mouths of 56 dogs among the 134 living in a French canine military training center (132e Groupe Cynophile de l'Armée de Terre, Suippes, France) were studied by use of enterobacterial ...

journal_title：Journal of clinical microbiology

authors： Loubinoux J,Lozniewski A,Lion C,Garin D,Weber M,Le Faou AE

更新日期：1999-08-01 00:00:00

abstract：:Candida parapsilosis is an increasing cause of bloodstream infections (BSIs) in neonatal intensive care units (NICUs). It has been a persistent problem in the NICU of Hospital for Children and Adolescents, Helsinki University Central Hospital, Helsinki, Finland, since 1987. Fluconazole prophylaxis has been used to con...

journal_title：Journal of clinical microbiology

authors： Sarvikivi E,Lyytikäinen O,Soll DR,Pujol C,Pfaller MA,Richardson M,Koukila-Kähkölä P,Luukkainen P,Saxén H

更新日期：2005-06-01 00:00:00

abstract：:We compared anaerobic lytic (AL) and pediatric aerobic resin-containing (Peds Plus/F) blood culture media for the isolation of Salmonella enterica serotype Typhi or Paratyphi A from children. The yields from AL and Peds Plus/F media were the same with equal volumes of blood, but recovery was faster from AL medium than...

journal_title：Journal of clinical microbiology

authors： Reller ME,Zaidi AK,Sultana S,Azeem S,Hanif B,Qureshi S,Hasan R,Bhutta Z,Akhter R,Goldmann DA

更新日期：2009-01-01 00:00:00

abstract：:D-Arabinitol (DA) is a useful diagnostic marker for candidiasis in patients with neutropenia and other high-risk groups, but its use in unselected patients with a broad range of underlying diseases and conditions has not been studied. We used an automated enzymatic fluorometric assay to measure serum DA/creatinine rat...

journal_title：Journal of clinical microbiology

authors： Yeo SF,Huie S,Sofair AN,Campbell S,Durante A,Wong B

更新日期：2006-11-01 00:00:00

abstract：:A 55-year-old man with severe peripheral vascular disease developed nosocomial septicemia which was caused by the gram-negative bacterium CDC group IV c-2, presumably from a plantar abscess on the left foot. Recovery followed amputation of the infected extremity and antibiotic therapy. This is the first reported case ...

journal_title：Journal of clinical microbiology

authors： Crowe HM,Brecher SM

更新日期：1987-11-01 00:00:00

abstract：:Antibiotic susceptibility studies were compared by the quantitative minimum inhibitory concentration and Autobac I. Four hundred common isolates from clinical materials including both gram-negative and -positive organisms were evaluated. Results demonstrate that the Autobac I method can provide reliable semiquantitati...

journal_title：Journal of clinical microbiology

authors： Jacob CV,Kleineman J

更新日期：1980-05-01 00:00:00

abstract：:During a 1-year period, the prevalence of thymidine-dependent (TD) Staphylococcus aureus in patients at two geographically distinct cystic fibrosis (CF) centers was determined. Of 200 CF patients who had their respiratory secretions cultured, 95 harbored S. aureus, and 20 (21%) had TD S. aureus as their predominant st...

journal_title：Journal of clinical microbiology

authors： Gilligan PH,Gage PA,Welch DF,Muszynski MJ,Wait KR

更新日期：1987-07-01 00:00:00