Lack of utility of the lysis-centrifugation blood culture method for detection of fungemia in immunocompromised cancer patients.

abstract：:In the last decade, the global emergence of carbapenem resistance in Enterobacteriaceae has posed great concern to public health. Data concerning the role of environmental contamination in the dissemination of carbapenem-resistant Enterobacteriaceae (CRE) are currently lacking. Here, we aimed to examine the extent of ...

journal_title：Journal of clinical microbiology

authors： Lerner A,Adler A,Abu-Hanna J,Meitus I,Navon-Venezia S,Carmeli Y

更新日期：2013-01-01 00:00:00

abstract：:The moderately repetitive sequence Ca3 was used to fingerprint strains of Candida albicans isolated from vulvovaginal infections of 10 women and strains isolated from their male partners. The Dendron software package was then used to compare the DNA fingerprints of these strains with those of vaginal commensals from w...

journal_title：Journal of clinical microbiology

authors： Schmid J,Rotman M,Reed B,Pierson CL,Soll DR

更新日期：1993-01-01 00:00:00

abstract：:Potential causes of congenital infection include Toxoplasma gondii and viruses such as cytomegalovirus (CMV), enterovirus, hepatitis C virus, herpes simplex virus types 1 and 2 (HSV-1 and -2), human herpesvirus types 6, 7, and 8, lymphocytic choriomeningitis virus, parvovirus, rubella virus, and varicella-zoster virus...

journal_title：Journal of clinical microbiology

authors： McIver CJ,Jacques CF,Chow SS,Munro SC,Scott GM,Roberts JA,Craig ME,Rawlinson WD

更新日期：2005-10-01 00:00:00

abstract：:We examined the blastogenic responses of lymphocytes to Staphylococcus aureus antigen in 49 uninfected non-insulin-dependent diabetic subjects and in 56 uninfected nondiabetic subjects. We found a significantly decreased response to this antigen in the diabetic patients (P less than 0.05). Diabetics whose blood sugar ...

journal_title：Journal of clinical microbiology

authors： Casey J,Sturm C Jr

更新日期：1982-01-01 00:00:00

abstract：:A series of clinical NDM-5-producing E. coli isolates obtained from two surveillance networks of carbapenem-producing Enterobacterales from 2018-2019, namely Switzerland (NARA) and Germany (SurvCARE), were analyzed. The 33 NDM-5-producing E. coli isolates were highly resistant to β-lactams including the novel ß-lactam...

journal_title：Journal of clinical microbiology

authors： Chakraborty T,Sadek M,Yao Y,Imirzalioglu C,Stephan R,Poirel L,Nordmann P

更新日期：2020-12-23 00:00:00

abstract：:The purpose of this study was to evaluate the performance of a herpes simplex virus (HSV) type 1-specific anti-glycoprotein C-1 monoclonal antibody (MAb) and a type 2-specific anti-glycoprotein G-2 MAb for typing of 2,400 clinical HSV-1 isolates and 2,400 clinical HSV-2 isolates, respectively, using an enzyme immunoas...

journal_title：Journal of clinical microbiology

authors： Liljeqvist JA,Svennerholm B,Bergström T

更新日期：1999-08-01 00:00:00

abstract：:Crude extracts of Dolichos biflorus can be coupled to polystyrene particles to yield an agglutination reagent for the detection of group C streptococcal antigen extracts. The reagent is relatively inexpensive and simple to prepare and can be employed for the definitive identification of this beta-hemolytic streptococc...

journal_title：Journal of clinical microbiology

authors： Slifkin M,Gil GM

更新日期：1984-01-01 00:00:00

abstract：:Fluorophore-assisted carbohydrate electrophoresis (FACE) is a straightforward, sensitive method for determining the presence and relative abundance of individual oligomannosyl residues in Candida mannoprotein, the major antigenic determinant located on the outer surface of the yeast cell wall. The single terminal alde...

journal_title：Journal of clinical microbiology

authors： Goins TL,Cutler JE

更新日期：2000-08-01 00:00:00

abstract：:Mycoplasma hominis most frequently causes diseases of the genitourinary tract. Extragenital infections are uncommon, with almost all occurring in immunosuppressed persons or those predisposed due to trauma or surgery. We present the case of a previously well man who developed an M. hominis-associated parapharyngeal ab...

journal_title：Journal of clinical microbiology

authors： Kennedy KJ,Prince S,Makeham T

更新日期：2009-09-01 00:00:00

abstract：:Several studies have identified nonsterile blood collection tubes as the reservoir for contaminating microorganisms in outbreaks of pseudobacteremia. Microorganisms are thought to be transferred from the nonsterile tubes to the blood culture tubes when blood is drawn for cultures, but the validity of this hypothesis h...

journal_title：Journal of clinical microbiology

authors： McNeil MM,Davis BJ,Anderson RL,Martone WJ,Solomon SL

更新日期：1985-07-01 00:00:00

abstract：:A novel recombinant baculovirus which expresses Ebola virus (EBO) nucleoprotein (NP) under the control of the cytomegalovirus immediate-early promoter was constructed. HeLa cells abortively infected with the baculovirus expressed EBO NP, and this was used as an immunofluorescent (IF) antigen to detect EBO immunoglobul...

journal_title：Journal of clinical microbiology

authors： Saijo M,Niikura M,Morikawa S,Kurane I

更新日期：2001-02-01 00:00:00

abstract：:PCR-restriction fragment length polymorphism analysis (PRA) using the novel region of the rpoB gene was developed for rapid and precise identification of mycobacteria to the species level. A total of 50 mycobacterial reference strains and 3 related bacterial strains were used to amplify the 360-bp region of rpoB, and ...

journal_title：Journal of clinical microbiology

authors： Lee H,Park HJ,Cho SN,Bai GH,Kim SJ

更新日期：2000-08-01 00:00:00

abstract：:Brevibacterium sp. was isolated from the blood of an acutely ill 18-year-old female with AIDS. The isolate was identified as Brevibacterium casei by use of carbohydrate assimilation tests. Treatment was successful with intravenously administered ciprofloxacin. To our knowledge, this is the first report of sepsis cause...

journal_title：Journal of clinical microbiology

authors： Brazzola P,Zbinden R,Rudin C,Schaad UB,Heininger U

更新日期：2000-09-01 00:00:00

abstract：:A premarket trial of the Becton Dickinson Directigen respiratory syncytial virus membrane-based enzyme immunoassay compared the test with virus isolation for the detection of respiratory syncytial virus in 583 nasopharyngeal aspirates. After modification, the Directigen test showed a sensitivity of 83% and a specifici...

journal_title：Journal of clinical microbiology

authors： Kok T,Barancek K,Burrell CJ

更新日期：1990-06-01 00:00:00

abstract：:Staphylococcal cassette chromosome mec (SCCmec) types and Panton-Valentine leukocidin (PVL) gene carriage were compared among suspected community-associated methicillin-resistant Staphylococcus aureus MRSA (CA-MRSA) and health care-associated MRSA (HA-MRSA) isolates. CA-MRSA isolates carried the SCCmec type IV complex...

journal_title：Journal of clinical microbiology

authors： Moroney SM,Heller LC,Arbuckle J,Talavera M,Widen RH

更新日期：2007-03-01 00:00:00

abstract：:Bordetella pertussis was diagnosed in a human immunodeficiency virus-infected patient by a newly developed method in which bacterial DNA is amplified directly from sputum Gram-stained slides. The validation of the method is described along with an additional new PCR-based assay that can distinguish between B. pertussi...

journal_title：Journal of clinical microbiology

authors： Vielemeyer O,Crouch JY,Edberg SC,Howe JG

更新日期：2004-02-01 00:00:00

abstract：:This report describes the results of an 11-laboratory study to determine if a cefoxitin broth microdilution MIC test could predict the presence of mecA in staphylococci. Using breakpoints of < or = 4 microg/ml for mecA-negative and > or = 6 or 8 microg/ml for mecA-positive isolates, sensitivity and specificity based o...

journal_title：Journal of clinical microbiology

authors： Swenson JM,Brasso WB,Ferraro MJ,Hardy DJ,Knapp CC,Lonsway D,McAllister S,Reller LB,Sader HS,Shortridge D,Skov R,Weinstein MP,Zimmer BL,Patel JB

更新日期：2009-06-01 00:00:00

abstract：:Visceral and cutaneous leishmaniases are heterogenous entities. The Leishmania species that a given patient harbors usually cannot be determined clinically, and this identification is essential to prescribe the best species-specific therapeutic regimen. Our diagnosis procedure includes a real-time PCR assay targeted a...

journal_title：Journal of clinical microbiology

authors： Foulet F,Botterel F,Buffet P,Morizot G,Rivollet D,Deniau M,Pratlong F,Costa JM,Bretagne S

更新日期：2007-07-01 00:00:00

abstract：:The actinomycete Rhodococcus equi is an important pathogen of horses and an emerging opportunistic pathogen of humans. Identification of R. equi by classical bacteriological techniques is sometimes difficult, and misclassification of an isolate is not uncommon. We report here on a specific PCR assay for the rapid and ...

journal_title：Journal of clinical microbiology

authors： Ladrón N,Fernández M,Agüero J,González Zörn B,Vázquez-Boland JA,Navas J

更新日期：2003-07-01 00:00:00

abstract：:Rapid diagnostic tests (RDTs) represent important tools to diagnose malaria infection. To improve understanding of the variable performance of RDTs that detect the major target in Plasmodium falciparum, namely, histidine-rich protein 2 (HRP2), and to inform the design of better tests, we undertook detailed mapping of ...

journal_title：Journal of clinical microbiology

authors： Lee N,Gatton ML,Pelecanos A,Bubb M,Gonzalez I,Bell D,Cheng Q,McCarthy JS

更新日期：2012-04-01 00:00:00

abstract：:We have developed a sensitive assay for Treponema pallidum subsp. pallidum (T. pallidum), the agent of veneral syphilis, based upon the polymerase chain reaction (PCR). A 658-bp portion of the gene encoding the 47-kDa membrane immunogen was amplified, and the PCR products were probed by DNA-DNA hybridization with a 49...

journal_title：Journal of clinical microbiology

authors： Burstain JM,Grimprel E,Lukehart SA,Norgard MV,Radolf JD

更新日期：1991-01-01 00:00:00

abstract：:The PathoTec Rapid I-D System for identifying Enterobacteriaceae was evaluated with 471 cultures. In 4,910 individual test comparisons, 95.5% of the results agreed, with results of only two test strips, those for esculin hydrolysis and urease production, agreeing with conventional tests in less than 94% of the trials....

journal_title：Journal of clinical microbiology

authors： Smith PB,Rhoden DL,Tomfohrde KM

更新日期：1975-04-01 00:00:00

abstract：:Diagnostic tests for respiratory viral infections have traditionally been performed on nasopharyngeal swabs or washings. Reverse transcriptase PCR (RT-PCR) is rapid, sensitive, and specific for viral infection diagnosis but is rarely applied to sputum samples. Thus, we evaluated the diagnostic yield of RT-PCR for dete...

journal_title：Journal of clinical microbiology

authors： Falsey AR,Formica MA,Walsh EE

更新日期：2012-01-01 00:00:00

abstract：:Spirochetes of the genus Treponema were cultured from 7 of 10 cases of digital dermatitis in sheep. Two cultures comprised Treponema phagedenis-like and Treponema medium/Treponema vincentii-like spirochetes, respectively, while the remaining cultures comprised mixed populations of Treponema medium/Treponema vincentii-...

journal_title：Journal of clinical microbiology

authors： Sayers G,Marques PX,Evans NJ,O'Grady L,Doherty ML,Carter SD,Nally JE

更新日期：2009-04-01 00:00:00

abstract：:A DNA macroarray containing 465 intragenic amplicons was designed to identify Staphylococcus aureus at the species level and to type S. aureus isolates. The genes selected included those encoding (i) S. aureus-specific proteins, (ii) staphylococcal and enterococcal proteins mediating antibiotic resistance and factors ...

journal_title：Journal of clinical microbiology

authors： Trad S,Allignet J,Frangeul L,Davi M,Vergassola M,Couve E,Morvan A,Kechrid A,Buchrieser C,Glaser P,El-Solh N

更新日期：2004-05-01 00:00:00

abstract：:Ehrlichia risticii causes an acute infectious disease in horses called Potomac horse fever. To investigate the biological diversity of E. risticii organisms, nine E. risticii isolates derived from the peripheral blood monocytes of clinically sick horses in Ohio and Kentucky during the summers of 1991 and 1993 were com...

journal_title：Journal of clinical microbiology

authors： Chaichanasiriwithaya W,Rikihisa Y,Yamamoto S,Reed S,Crawford TB,Perryman LE,Palmer GH

更新日期：1994-12-01 00:00:00

abstract：:Rotavirus is the most common cause of severe dehydrating gastroenteritis in infants. To date, 10 different serotypes of rotavirus have been identified in human stools. While four or five serotypes dominate, serotype circulation varies with season and geography. Since our laboratory has been involved in the development...

journal_title：Journal of clinical microbiology

authors： DiStefano DJ,Kraiouchkine N,Mallette L,Maliga M,Kulnis G,Keller PM,Clark HF,Shaw AR

更新日期：2005-12-01 00:00:00

abstract：:A swine H3N2 (swH3N2) and pandemic (H1N1) 2009 (pH1N1) influenza A virus reassortant (swH3N2/pH1N1) was detected in Canadian swine at the end of 2010. Simultaneously, a similar virus was also detected in Canadian mink based on partial viral genome sequencing. The origin of the new swH3N2/pH1N1 viral genes was related ...

journal_title：Journal of clinical microbiology

authors： Tremblay D,Allard V,Doyon JF,Bellehumeur C,Spearman JG,Harel J,Gagnon CA

更新日期：2011-12-01 00:00:00

abstract：:Many bacterial typing methods are specific for one species only, time-consuming, or poorly reproducible. DiversiLab (DL; bioMérieux) potentially overcomes these limitations. In this study, we evaluated the DL system for the identification of hospital outbreaks of a number bacterial species. Appropriately typed clinica...

journal_title：Journal of clinical microbiology

authors： Fluit AC,Terlingen AM,Andriessen L,Ikawaty R,van Mansfeld R,Top J,Cohen Stuart JW,Leverstein-van Hall MA,Boel CH

更新日期：2010-11-01 00:00:00

abstract：:Broth microdilution and disk diffusion susceptibility testing were performed on 73 strains of Pasteurella species isolated from human infections and on five American Type Culture Collection strains of Pasteurella species. Both methods appear reliable for testing susceptibilities of Pasteurella species. ...

journal_title：Journal of clinical microbiology

authors： Citron DM,Warren YA,Fernandez HT,Goldstein MA,Tyrrell KL,Goldstein EJ

更新日期：2005-05-01 00:00:00