Identification of Bordetella pertussis in a critically ill human immunodeficiency virus-infected patient by direct genotypical analysis of Gram-stained material and discrimination from B. holmesii by using a unique recA gene restriction enzyme site.

abstract：:The polymerase chain reaction (PCR) has many potential applications in the field of nucleic acid diagnostics. In particular, it has been successfully applied to the detection of pathogens present in low copy numbers such as the human immunodeficiency virus type 1. Here we describe a time-resolved fluorescence-based hy...

journal_title：Journal of clinical microbiology

authors： Dahlén PO,Iitiä AJ,Skagius G,Frostell A,Nunn MF,Kwiatkowski M

更新日期：1991-04-01 00:00:00

abstract：:We compared the new Abbott TestPack (TP) respiratory syncytial virus (RSV) enzyme immunoassay (EIA) with cell culture and two commercial RSV EIAs (from Abbott Diagnostics and Kallestad Laboratories) by using split samples of fresh nasal washings from children with suspected RSV disease. Two tubes of HEp-2 cells were i...

journal_title：Journal of clinical microbiology

authors： Wren CG,Bate BJ,Masters HB,Lauer BA

更新日期：1990-06-01 00:00:00

abstract：:Sequencing-based pathogen identification directly from clinical specimens requires time-consuming interpretation, especially with mixed chromatograms when multiple microorganisms are detected. We assessed RipSeq Mixed software for human papillomavirus (HPV) genotyping by comparison to the linear array HPV genotyping a...

journal_title：Journal of clinical microbiology

authors： Tardif KD,Simmon KE,Kommedal O,Pyne MT,Schlaberg R

更新日期：2013-04-01 00:00:00

abstract：:The 30-micrograms cefprozil disk and the cephalosporin class (30-micrograms cephalothin) disk were compared for their abilities to predict cefprozil susceptibility by agar disk diffusion testing. High error (5.02% major and 14.11% minor) rates were encountered with the cephalothin disk and were most frequently observe...

journal_title：Journal of clinical microbiology

authors： Fung-Tomc J,Stickle T,Doyle C,Huczko E,Kessler RE

更新日期：1991-11-01 00:00:00

abstract：:Variable-number tandem repeats (VNTRs) may evolve so rapidly that multiple profiles emerge during an outbreak. A total of 190 isolates from eight Salmonella enterica serovar Typhimurium outbreaks and 15 isolates from seven patients were analyzed by pulsed-field gel electrophoresis and VNTR typing. Small changes in loc...

journal_title：Journal of clinical microbiology

authors： Hopkins KL,Maguire C,Best E,Liebana E,Threlfall EJ

更新日期：2007-09-01 00:00:00

abstract：:A simple and reliable technique was developed for differentiating Helicobacter pylori strains by restriction fragment length polymorphism analysis of polymerase chain reaction (PCR)-amplified DNAs. Oligonucleotide primer pairs developed to the urease, 48-kDa stress protein (htrA), and 26-kDa antigen-encoding genes wer...

journal_title：Journal of clinical microbiology

authors： Clayton CL,Kleanthous H,Morgan DD,Puckey L,Tabaqchali S

更新日期：1993-06-01 00:00:00

abstract：:Nontuberculous mycobacterial lymphadenitis presents an increasing clinical problem in immunocompetent young children. A slowly growing, nonphotochromogenic mycobacterium was recovered twice (isolates 2553/91 and 2554/91) from the lymphatic tissue of a child with recurrent cervical lymphadenitis. It could be differenti...

journal_title：Journal of clinical microbiology

authors： Haas WH,Butler WR,Kirschner P,Plikaytis BB,Coyle MB,Amthor B,Steigerwalt AG,Brenner DJ,Salfinger M,Crawford JT,Böttger EC,Bremer HJ

更新日期：1997-12-01 00:00:00

abstract：:A multiplexed biomarker bundle consisting of nine different inflammation markers was evaluated regarding their diagnostic and prognostic performances in 159 adult systemic inflammatory response syndrome (SIRS) patients enrolled at the emergency department. Fibronectin, interleukin-8 (IL-8), biotin, and neutrophil gela...

journal_title：Journal of clinical microbiology

authors： Reichsoellner M,Raggam RB,Wagner J,Krause R,Hoenigl M

更新日期：2014-11-01 00:00:00

abstract：:A singleplex PCR assay using a single primer pair targeting the putative sugar transporter gene was developed here to distinguish Cryptococcus neoformans var. grubii, Cryptococcus neoformans var. neoformans, and Cryptococcus gattii according to the distinct size of the amplicon. The interspecies and intravarietal hybr...

journal_title：Journal of clinical microbiology

authors： Feng X,Fu X,Ling B,Wang L,Liao W,Yao Z

更新日期：2013-06-01 00:00:00

abstract：:We compared the results of Gram staining and culture of cerebrospinal fluid to results obtained with a rapid PCR assay for the diagnosis of meningococcal meningitis in 281 cases of suspected bacterial meningitis. PCR had a sensitivity of 97% compared to a sensitivity of 55% for culture, and the PCR specificity was 99....

journal_title：Journal of clinical microbiology

authors： Richardson DC,Louie L,Louie M,Simor AE

更新日期：2003-08-01 00:00:00

abstract：:Trichosporon species are relevant etiologic agents of hospital-acquired infections. High mortality rates are attributed to Trichosporon deep-seated infections in immunocompromised individuals, making fast and accurate species identification relevant for hastening the discovery of best-targeted therapy. Recently, Trich...

journal_title：Journal of clinical microbiology

authors： de Almeida JN Jr,Favero Gimenes VM,Francisco EC,Machado Siqueira LP,Gonçalves de Almeida RK,Guitard J,Hennequin C,Colombo AL,Benard G,Rossi F

更新日期：2017-08-01 00:00:00

abstract：:We describe a central venous catheter-related (Port-A-Cath; Smiths Industries Medical Systems [SIMS] Deltec, Inc., St. Paul, Minn.) infection caused by Rhodotorula glutinis in a 51-year-old man with nasopharyngeal carcinoma. He was treated with fluconazole for 8 weeks and had the catheter removed. Two isolates of R. g...

journal_title：Journal of clinical microbiology

authors： Hsueh PR,Teng LJ,Ho SW,Luh KT

更新日期：2003-02-01 00:00:00

abstract：:We compared the Abbott enzyme immunoassay for human immunodeficiency virus (HIV) antigen with the reverse transcriptase assay (RTA) as a means of monitoring HIV infection during an antiviral trial. The Abbott enzyme immunoassay detected HIV earlier than RTA whether or not the patients were antigenemic and appears to b...

journal_title：Journal of clinical microbiology

authors： Burger H,Paul D,Siegal FP,Wendel I,Neff S,Eilbott D,Gehan K,Grimson R,Weiser B

更新日期：1988-09-01 00:00:00

abstract：:Since influenza viruses can cause severe illness, timely diagnosis is important for an adequate intervention. The available rapid detection methods either lack sensitivity or require complex laboratory manipulation. This study describes a rapid, sensitive detection method that can be easily applied to routine diagnosi...

journal_title：Journal of clinical microbiology

authors： van Elden LJ,Nijhuis M,Schipper P,Schuurman R,van Loon AM

更新日期：2001-01-01 00:00:00

abstract：:Restriction fragment length polymorphism and hybridization of DNA extracted from Mycobacterium tuberculosis, nontuberculous mycobacteria, and nonmycobacterial species with a probe derived from IS6110 confirmed that IS6110 was specific to M. tuberculosis complex. In addition, DNA amplification with IS6110-specific prim...

journal_title：Journal of clinical microbiology

authors： Githui WA,Wilson SM,Drobniewski FA

更新日期：1999-04-01 00:00:00

abstract：:Three strains of a Pasteurella haemolytica-like bacterium were isolated from lesions of a progressive granuloma of cattle in southern Brazil. Their characteristics and their differentiation from P. haemolytica varieties and Actinobacillus lignieresii are described. The name "Pasteurella granulomatis" is proposed for t...

journal_title：Journal of clinical microbiology

authors： Ribeiro GA,Carter GR,Frederiksen W,Riet-Correa F

更新日期：1989-06-01 00:00:00

abstract：:An estimated 41,200 people were newly infected with hepatitis C virus (HCV) in 2016 in the United States. Screening tests for antibodies to HCV may generate up to 32% false positivity in low-risk populations. Current Centers for Disease Control and Prevention (CDC) screening recommendations do not require confirmatory...

journal_title：Journal of clinical microbiology

authors： Kodani M,Martin M,de Castro VL,Drobeniuc J,Kamili S

更新日期：2019-02-27 00:00:00

abstract：:Members of the genus Malassezia, lipophilic yeasts, are considered to be one of the exacerbating factors in atopic dermatitis (AD). We examined variation in cutaneous colonization by Malassezia species in AD patients and compared it with variation in healthy subjects. Samples were collected by applying transparent dre...

journal_title：Journal of clinical microbiology

authors： Sugita T,Suto H,Unno T,Tsuboi R,Ogawa H,Shinoda T,Nishikawa A

更新日期：2001-10-01 00:00:00

abstract：:Four of eleven affected dogs died despite aggressive treatment during a 2015 focal outbreak of hemorrhagic gastroenteritis following a stay in a pet housing facility. Routine diagnostic investigations failed to identify a specific cause. Virus isolation from fresh necropsy tissues yielded a calicivirus with sequence h...

journal_title：Journal of clinical microbiology

authors： Renshaw RW,Griffing J,Weisman J,Crofton LM,Laverack MA,Poston RP,Duhamel GE,Dubovi EJ

更新日期：2018-04-25 00:00:00

abstract：:Two cyp51-related genes (cyp51A and cyp51B) encoding 14-alpha sterol demethylase-like enzymes were identified in the opportunistic human pathogen Aspergillus fumigatus. PCR amplification using degenerate oligonucleotides based on conserved areas of cytochrome P450 demethylases of other filamentous fungi and yeasts all...

journal_title：Journal of clinical microbiology

authors： Mellado E,Diaz-Guerra TM,Cuenca-Estrella M,Rodriguez-Tudela JL

更新日期：2001-07-01 00:00:00

abstract：:Quantitative real-time PCR (qPCR) is increasingly being used for the detection of bovine leukemia virus (BLV) proviral DNA. Nevertheless, quality control for the validation and standardization of such tests is currently lacking. Therefore, the present study was initiated by three Office International des Epizooties (O...

journal_title：Journal of clinical microbiology

authors： Jaworski JP,Pluta A,Rola-Łuszczak M,McGowan SL,Finnegan C,Heenemann K,Carignano HA,Alvarez I,Murakami K,Willems L,Vahlenkamp TW,Trono KG,Choudhury B,Kuźmak J

更新日期：2018-06-25 00:00:00

abstract：:A serotyping scheme for Enterobacter cloacae based on heat-stable somatic antigens is described. A total of 28 antisera were prepared in rabbits, and titers of agglutinins were high (greater than 640). Some cross-reactions were observed, and 11 sera required absorption before routine use. Of 300 clinical isolates from...

journal_title：Journal of clinical microbiology

authors： Gaston MA,Bucher C,Pitt TL

更新日期：1983-11-01 00:00:00

abstract：:To compare Simplexa Flu A/B & RSV PCR with cytospin-immunofluorescence and laboratory-developed TaqMan PCR methods, 445 nasopharyngeal samples were tested. Of these, 199 were positive (46 for respiratory syncytial virus [RSV], 120 for influenza A, and 33 for influenza B) and 246 were negative. The direct fluorescent-a...

journal_title：Journal of clinical microbiology

authors： Landry ML,Ferguson D

更新日期：2014-08-01 00:00:00

abstract：:The rapid ID32A kit (bioMérieux Vitek, Inc., Hazelwood, Mo.) was evaluated for its ability to identify Peptostreptococcus species compared with conventional biochemical tests and gas-liquid chromatography (Virginia Polytechnic Institute), the current "gold standard" method. A total of 5 Peptostreptococcus American Typ...

journal_title：Journal of clinical microbiology

authors： Ng J,Ng LK,Chow AW,Dillon JA

更新日期：1994-05-01 00:00:00

abstract：:Aspergillus terreus isolates collected from patients at The M. D. Anderson Cancer Center in Houston, TX, and at The University Hospital of Innsbruck, Austria, were analyzed using random amplification of polymorphic DNA-PCR with three different primers. No strain similarity in either institution was detected, indicatin...

journal_title：Journal of clinical microbiology

authors： Lass-Flörl C,Grif K,Kontoyiannis DP

更新日期：2007-08-01 00:00:00

abstract：:The sensitivity of PCR for the amplification of target nucleic acid sequences in clinical diagnostics may often be reduced due to the presence of inhibitory factors. Hemolytic serum contains a number of PCR inhibitors, one of which is hemin. In this study we have found that conventional methods of DNA extraction were ...

journal_title：Journal of clinical microbiology

authors： Klein A,Barsuk R,Dagan S,Nusbaum O,Shouval D,Galun E

更新日期：1997-07-01 00:00:00

abstract：:A total of 189 Candida albicans isolates have been typed by multilocus enzyme electrophoresis. The results obtained confirm the clonal mode of reproduction of C. albicans. The C. albicans populations found in the oropharynx of human immunodeficiency virus (HIV)-infected patients, in the oropharynx of healthy carriers,...

journal_title：Journal of clinical microbiology

authors： Boerlin P,Boerlin-Petzold F,Goudet J,Durussel C,Pagani JL,Chave JP,Bille J

更新日期：1996-05-01 00:00:00

abstract：:Explanted orthopedic implants from 54 patients with aseptic failure and 24 patients with prosthetic knee or hip infection were sonicated in polyethylene bags. The sensitivities of periprosthetic tissue and sonicate fluid cultures for the diagnosis of prosthetic joint infection were 54% and 75%, whereas the specificiti...

journal_title：Journal of clinical microbiology

authors： Trampuz A,Piper KE,Hanssen AD,Osmon DR,Cockerill FR,Steckelberg JM,Patel R

更新日期：2006-02-01 00:00:00

abstract：:The prevalence of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli (ESBLEC) in Spain increased 8-fold from 2000 to 2006. ESBL type, clonal relationship, antimicrobial susceptibility, and clinical data about infections caused by ESBLEC are evaluated in a second nationwide study developed in 2006. From...

journal_title：Journal of clinical microbiology

authors： Díaz MA,Hernández-Bello JR,Rodríguez-Baño J,Martínez-Martínez L,Calvo J,Blanco J,Pascual A,Spanish Group for Nosocomial Infections (GEIH).

更新日期：2010-08-01 00:00:00

abstract：:An evaluation was undertaken to determine the optimal method for the in vitro susceptibility testing of 26 Nocardia asteroides complex isolates to the following antimicrobial agents: amikacin, ampicillin, amoxicillin-clavulanate, ceftriaxone, ciprofloxacin, erythromycin, imipenem, minocycline, and trimethoprim-sulfame...

journal_title：Journal of clinical microbiology

authors： Ambaye A,Kohner PC,Wollan PC,Roberts KL,Roberts GD,Cockerill FR 3rd

更新日期：1997-04-01 00:00:00