Abstract:
:The PathoTec Rapid I-D System for identifying Enterobacteriaceae was evaluated with 471 cultures. In 4,910 individual test comparisons, 95.5% of the results agreed, with results of only two test strips, those for esculin hydrolysis and urease production, agreeing with conventional tests in less than 94% of the trials. The PathoTec system exhibited 94.3% accuracy in identifying these cultures in a double-blind study with conventional media and procedures as the alternate system. Two newly developed test strips, for 0-nitrophenyl-beta-D-galactopyranoside and ornithine decarboxylase, were found to be highly reliable.
journal_name
J Clin Microbioljournal_title
Journal of clinical microbiologyauthors
Smith PB,Rhoden DL,Tomfohrde KMdoi
10.1128/JCM.1.4.359-362.1975keywords:
subject
Has Abstractpub_date
1975-04-01 00:00:00pages
359-62issue
4eissn
0095-1137issn
1098-660Xjournal_volume
1pub_type
临床试验,杂志文章abstract::Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) was collected from children with bullous impetigo in 2003 and 2004. One strain collected in 2003 was Panton-Valentine leucocidin (PVL) positive. In 2004, a multiple-drug-resistant PVL(+) CA-MRSA strain was isolated from an athlete with a cutaneou...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.43.7.3356-3363.2005
更新日期:2005-07-01 00:00:00
abstract::In order to complete the immunodiagnosis of human toxocaral disease, an immunoenzymatic assay with excretory-secretory antigens from Toxocara canis larvae was developed for the detection of specific immunoglobulin E (sIgE enzyme-linked immunosorbent assay [ELISA]). The specificity of the assay was evaluated in patient...
journal_title:Journal of clinical microbiology
pub_type: 临床试验,杂志文章
doi:10.1128/JCM.30.9.2269-2274.1992
更新日期:1992-09-01 00:00:00
abstract::Helcococcus kunzii, a nonvirulent member of the human skin flora, has recently been implicated in causing infections in immunosuppressed patients. We report a case of breast abscess associated with H. kunzii in an immunocompetant patient and discuss the criteria used in its identification and our observations of susce...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.36.8.2377-2379.1998
更新日期:1998-08-01 00:00:00
abstract::During a 15-month period, two methods for detection of herpes simplex virus (HSV) in 699 clinical specimens were compared: (i) 24-well-plate centrifugation (24WPC) with MRC-5 cells and staining with type-specific monoclonal antibodies (Syva Co., Palo Alto, Calif.) after incubation for 16 to 18 h and (ii) conventional ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.26.3.570-572.1988
更新日期:1988-03-01 00:00:00
abstract::An in-house enzyme-linked immunosorbent assay (ELISA) for measurement of Helicobacter pylori-specific immunoglobulin G (IgG) and IgA in saliva was evaluated by comparison with histopathologic (Giemsa staining) and biochemical (urease quick test) examination of gastric biopsy specimens obtained from 112 children referr...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.35.12.3358-3360.1997
更新日期:1997-12-01 00:00:00
abstract::Monoclonal antibodies to Staphylococcus aureus capsular polysaccharide types 5 and 8 were used in an enzyme-linked immunosorbent assay to serotype 74 and 42 coagulase-negative isolates from cow and goat milk, respectively. Eighteen (15.5%) isolates were typable: 13 Staphylococcus haemolyticus, 1 S. hyicus, 1 S. simula...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.28.2.358-360.1990
更新日期:1990-02-01 00:00:00
abstract::A variety of bacterins, vaccines, and antisera retained greater than 90% of their original level of mercurial preservative after lyophilization, and this might influence certain uses of these products. ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:
更新日期:1975-12-01 00:00:00
abstract::A rapid dimethyl sulfoxide modification of an acid-fast technique was applied to direct fecal smears to monitor cryptosporidiosis in nonhuman primates. Brilliantly stained pink oocysts against a pale green background demonstrated well-preserved internal morphology and facilitated rapid, simple, noninvasive diagnosis w...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.19.6.952-953.1984
更新日期:1984-06-01 00:00:00
abstract::Current methods for the diagnosis of melioidosis are based on bacteriological culture. A number of serological tests currently available lack specificity and sensitivity. This is largely due to the use of crude antigens which results in a significant cross-reactivity with sera from individuals infected with other bact...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.31.5.1232-1236.1993
更新日期:1993-05-01 00:00:00
abstract::A multiplex method using the SNaPshot technique was developed to screen for six common mycobacterial species: Mycobacteria tuberculosis, M. avium, M. intracellulare, M. chelonae, M. kansasii, and M. gordonae. A total of 468 mycobacterial clinical isolates were subjected to analysis for the presence of the six mycobact...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01084-09
更新日期:2010-01-01 00:00:00
abstract::Vaginal swab specimens may be preferable to cervical swab or urine specimens for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae because of the ease of specimen collection and transport. The purpose of this study was to evaluate whether vaginal swab specimens are equivalent to cervical swab specimens ...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/jcm.41.8.3592-3596.2003
更新日期:2003-08-01 00:00:00
abstract::We developed a liquid bead microarray (LBMA) assay for genotyping genital human papillomaviruses (HPVs) based on the MY09-MY11-HMB01 PCR system and the reverse line blot (RLB) assay probe sequences. Using individual HPV plasmids, we were able to detect as few as 50 copies per reaction. In two separate retrospective st...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01707-08
更新日期:2009-03-01 00:00:00
abstract::INTRODUCTIONSyndromic panels were first FDA cleared for detection of respiratory pathogens in 2008. Since then, other panels have been approved by the FDA, and most recently, the FilmArray meningitis/encephalitis panel (BioFire, Salt Lake City, UT) has become available. This assay detects 14 targets within 1 h and inc...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00018-18
更新日期:2018-03-26 00:00:00
abstract::Coxsackievirus (CV) strains CVA2, CVA4, CVA5, and CVA10 were isolated from patients with hand, foot, and mouth disease during a 2009 outbreak in China. Full genome sequences for four representative strains, CVA2/SD/CHN/09 (A2SD09), CVA4/SZ/CHN/09 (A4SZ09), CVA5/SD/CHN/09 (A5SD09), and CVA10/SD/CHN/09 (A10SD09), were d...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00007-11
更新日期:2011-07-01 00:00:00
abstract::Human herpesvirus 6 (HHV-6) variant B is frequently identified in peripheral blood, but identification of HHV-6 variant A is relatively rare. We devised a PCR-based method for sensitive, simultaneous detection of both HHV-6 variants. The method was applied to 34 lung tissue specimens that were previously shown to cont...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.34.4.877-881.1996
更新日期:1996-04-01 00:00:00
abstract::A simple and reliable technique was developed for differentiating Helicobacter pylori strains by restriction fragment length polymorphism analysis of polymerase chain reaction (PCR)-amplified DNAs. Oligonucleotide primer pairs developed to the urease, 48-kDa stress protein (htrA), and 26-kDa antigen-encoding genes wer...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.31.6.1420-1425.1993
更新日期:1993-06-01 00:00:00
abstract::We describe here the use of an immunomagnetic separation enrichment process coupled with a modified real-time cellular analysis (RTCA) system (RTCA version 2) for the detection of C. difficile toxin (CDT) in stool. The limit of CDT detection by RTCA version 2 was 0.12 ng/ml. Among the consecutively collected 401 diarr...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.02601-13
更新日期:2014-04-01 00:00:00
abstract::Laboratory and clinical diagnostic classification of seropositive individuals, followed by treatment and supportive therapy, is an established component of Chagas' disease control in areas where this disease is endemic. However, most Chagas' disease patients live in remote areas where neither equipped laboratories nor...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.02303-07
更新日期:2008-06-01 00:00:00
abstract::The past 2 decades have brought worrying increases in severe Streptococcus pyogenes diseases globally. To investigate and compare the epidemiological patterns of these diseases within Europe, data were collected through a European Union FP-5-funded program (Strep-EURO). Prospective population-based surveillance of sev...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.00422-08
更新日期:2008-07-01 00:00:00
abstract::Three hundred ninety single sera from adults in Japan, Indonesia, Singapore, and Papua New Guinea were tested for antibodies to human calicivirus (HCV) by a radioimmunoassay blocking test. A high prevalence of antibodies was observed in samples collected in Japan and Southeast Asia. Of 240 serum specimens collected fr...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.22.4.519-521.1985
更新日期:1985-10-01 00:00:00
abstract::In comparative studies, Hemo-De (PMP Medical Industries, Inc., Irving, Tex.) was found to be a suitable replacement for ethyl acetate in the Formalin-ethyl acetate concentration technique. With essentially equivalent recovery rates for both procedures, the Formalin-Hemo-De concentration technique is considered to be t...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.25.2.425-426.1987
更新日期:1987-02-01 00:00:00
abstract::Alpha-toxin is a major Staphylococcus aureus virulence factor. This study evaluated potential relationships between in vitro alpha-toxin expression of S. aureus bloodstream isolates, anti-alpha-toxin antibody in serum of patients with S. aureus bacteremia (SAB), and clinical outcomes in 100 hemodialysis and 100 postsu...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.02023-14
更新日期:2015-01-01 00:00:00
abstract::The capsular-like envelope of Legionella pneumophila strains Togus 1 (serotype 2) and Philadelphia 1 (serotype 1) was isolated and purified by column chromatography on Sepharose 6B. Antibody raised in rabbits to these two antigenic materials did not cross-react in gel diffusion. Upon electrophoresis followed by gel di...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.13.4.637-642.1981
更新日期:1981-04-01 00:00:00
abstract::Southern blot hybridization with the Ca3 probe and the C fragment of the Ca3 probe was used to assess the genetic relatedness of Candida albicans strains from one patient with recurrent C. albicans infection in whom the same strain was maintained, one patient in whom the infecting strain was replaced, and their male s...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.32.11.2646-2654.1994
更新日期:1994-11-01 00:00:00
abstract::The present study describes a method for amplifying DNA in Actinobacillus actinomycetemcomitans by using short, synthetic oligonucleotides of random sequence as primers in the polymerase chain reaction. Genomic DNA from each of 20 human isolates of A. actinomycetemcomitans was successfully amplified in a thermal cycle...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.31.10.2773-2776.1993
更新日期:1993-10-01 00:00:00
abstract::A serological study was performed to determine recent human exposure to Burkholderia pseudomallei (the cause of melioidosis) in residents of southern Thailand affected by the tsunami of 26 December 2004. The findings were suggestive of increased recent exposure in both tsunami survivors and uninjured bystanders. Survi...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.44.1.239-240.2006
更新日期:2006-01-01 00:00:00
abstract::We have evaluated the diagnostic utility of eleven Toxoplasma gondii recombinant antigens (P22 [SAG2], P24 [GRA1], P25, P28 [GRA2], P29 [GRA7], P30 [SAG1], P35, P41 [GRA4], P54 [ROP2], P66 [ROP1], and P68) in immunoglobulin G (IgG) and IgM recombinant enzyme-linked immunosorbent assays (Rec-ELISAs). Following an initi...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.38.3.1144-1150.2000
更新日期:2000-03-01 00:00:00
abstract::Viral hemorrhagic fevers, because of their high mortality rates, the lack of medical countermeasures, and their potential use as instruments of bioterrorism, pose a significant threat to the developed and the developing areas of the world. The key to preventing the spread of these diseases is early and accurate detect...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.01693-16
更新日期:2016-12-28 00:00:00
abstract::Cag pathogenicity island-containing Helicobacter pylori (type I) induces signal transduction pathways resulting in tyrosine phosphorylation of proteins adjacent to the site of bacterial adhesion on host gastric epithelial cells. Conventional block PCR-restriction fragment length polymorphism (RFLP) and real-time Light...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/jcm.41.7.3112-3118.2003
更新日期:2003-07-01 00:00:00
abstract::The performance of four media for the isolation of Aeromonas strains from stool specimens, the importance of ampicillin-susceptible Aeromonas strains in the selection of culture media, and the usefulness of beta-hemolysis in screening blood-containing media for Aeromonas strains were evaluated in two phases. In the fi...
journal_title:Journal of clinical microbiology
pub_type: 杂志文章
doi:10.1128/JCM.26.9.1738-1740.1988
更新日期:1988-09-01 00:00:00