A Panton-Valentine leucocidin (PVL)-positive community-acquired methicillin-resistant Staphylococcus aureus (MRSA) strain, another such strain carrying a multiple-drug resistance plasmid, and other more-typical PVL-negative MRSA strains found in Japan.

abstract：:A prospective study was conducted on 25 Legionella pneumophila culture-positive and 98 culture-negative bronchoalveolar lavage fluid samples to compare two DNA preparation methods: a rapid modified Chelex-based protocol and a proteinase K method. PCR was found to be more sensitive with the Chelex-based method (P = 0.0...

journal_title：Journal of clinical microbiology

authors： Jaulhac B,Reyrolle M,Sodahlon YK,Jarraud S,Kubina M,Monteil H,Piémont Y,Etienne J

更新日期：1998-07-01 00:00:00

abstract：:A new principle that uses anti-immunoglobulin M-coated polystyrene microtiter plates for the detection of immunoglobulin M antibodies against Toxoplasma gondii by an enzyme-linked immunosorbent assay was developed. ...

journal_title：Journal of clinical microbiology

authors： Duermeyer W,Wielaard F,van Gruijthuijsen H,Swinkels J

更新日期：1980-12-01 00:00:00

abstract：:The object of this study was to develop a test that would measure antibodies directed against group B streptococcal types Ia, Ib, Ic, II, and III. The type-specific carbohydrate antigens were purified, labeled with 125I, and used to develop a radioimmunoassay. This procedure should be particularly useful in testing hu...

journal_title：Journal of clinical microbiology

authors： Wilkinson HW,Jones WL

更新日期：1976-05-01 00:00:00

abstract：:In order to investigate the seroprevalence of human herpesvirus 8 (HHV-8) infection in central and southern Italy, sera from human immunodeficiency virus (HIV)-seronegative subjects, with and without Kaposi's sarcoma (KS), were analyzed by immunofluorescence assay, using BC-3, a cell line latently infected with HHV-8....

journal_title：Journal of clinical microbiology

authors： Cattani P,Capuano M,Cerimele F,La Parola IL,Santangelo R,Masini C,Cerimele D,Fadda G

更新日期：1999-04-01 00:00:00

abstract：:During an outbreak of diarrheal illness among residents of a trailer park in rural Vermont, 37 (30%) of 122 residents met the case definition of outbreak-related giardiasis. Convalescent-phase sera from 24 residents and 20 nonresident control subjects were tested by enzyme-linked immunosorbent assay for immunoglobulin...

journal_title：Journal of clinical microbiology

authors： Birkhead G,Janoff EN,Vogt RL,Smith PD

更新日期：1989-08-01 00:00:00

abstract：:We reviewed laboratory-acquired infections occurring in Utah from 1978 through 1982. Written and telephone interviews of supervisors of 1,191 laboratorians revealed an estimated annual incidence of 3 laboratory-acquired infections per 1,000 employees. Infections, in order of frequency, included hepatitis B (clinical c...

journal_title：Journal of clinical microbiology

authors： Jacobson JT,Orlob RB,Clayton JL

更新日期：1985-04-01 00:00:00

abstract：:Rapid tests for detection of methicillin-resistant Staphylococcus aureus (MRSA) carriage are important to limit the transmission of MRSA in the health care setting. We evaluated the performance of the BD GeneOhm MRSA real-time PCR assay using a diverse collection of MRSA isolates, mainly from Copenhagen, Denmark, but ...

journal_title：Journal of clinical microbiology

authors： Bartels MD,Boye K,Rohde SM,Larsen AR,Torfs H,Bouchy P,Skov R,Westh H

更新日期：2009-05-01 00:00:00

abstract：:Cultures of Sporothrix schenckii were serologically tested by the exoantigen immunodiffusion technique of Kaufman and Standard (L. Kaufman and P.G. Standard, J. Clin. Microbiol. 8:42-45, 1978). This rapid and sensitive technique permitted the identification of 10 isolates of S. schenckii within 3 days. The production ...

journal_title：Journal of clinical microbiology

authors： Polonelli L,Morace G

更新日期：1982-03-01 00:00:00

abstract：:Aspirates have been cultured from 10 dental abscesses of endodontic origin, all of which had penetrated beyond the bony alveolus to produce fluctuant swelling. Sampling was by syringe aspiration. Strict anaerobic techniques, including the use of an anaerobic chamber, were used for serial dilution and plating. Randomly...

journal_title：Journal of clinical microbiology

authors： Williams BL,McCann GF,Schoenknecht FD

更新日期：1983-10-01 00:00:00

abstract：:A multiplexed biomarker bundle consisting of nine different inflammation markers was evaluated regarding their diagnostic and prognostic performances in 159 adult systemic inflammatory response syndrome (SIRS) patients enrolled at the emergency department. Fibronectin, interleukin-8 (IL-8), biotin, and neutrophil gela...

journal_title：Journal of clinical microbiology

authors： Reichsoellner M,Raggam RB,Wagner J,Krause R,Hoenigl M

更新日期：2014-11-01 00:00:00

abstract：:Explanted orthopedic implants from 54 patients with aseptic failure and 24 patients with prosthetic knee or hip infection were sonicated in polyethylene bags. The sensitivities of periprosthetic tissue and sonicate fluid cultures for the diagnosis of prosthetic joint infection were 54% and 75%, whereas the specificiti...

journal_title：Journal of clinical microbiology

authors： Trampuz A,Piper KE,Hanssen AD,Osmon DR,Cockerill FR,Steckelberg JM,Patel R

更新日期：2006-02-01 00:00:00

abstract：:Direct enzyme immunoassay (Herpchek) was compared with culture on 21,522 specimens mainly from asymptomatic women for herpes simplex virus detection. Sensitivity and specificity were 73.8 and 97.7%, respectively. The 33% detection rate by enzyme immunoassay in 5 h increased to 43% when the enzyme immunoassay was combi...

journal_title：Journal of clinical microbiology

authors： Verano L,Michalski FJ

更新日期：1995-05-01 00:00:00

abstract：:The Abbott RealTime MTB (RT MTB) assay is a new automated nucleic acid amplification test for the detection of Mycobacterium tuberculosis complex (MTBC) in clinical specimens. In combination with the RealTime MTB INH/RIF (RT MTB INH/RIF) resistance assay, which can be applied to RT MTB-positive specimens as an add-on ...

journal_title：Journal of clinical microbiology

authors： Hofmann-Thiel S,Molodtsov N,Antonenka U,Hoffmann H

更新日期：2016-12-01 00:00:00

abstract：:The Lyme disease-associated spirochete, Borrelia burgdorferi, is maintained in enzootic cycles involving Ixodes ticks and small mammals. Previous studies demonstrated that B. burgdorferi expresses outer surface protein A (OspA) but not OspC when residing in the midgut of unfed ticks. However, after ticks feed on blood...

journal_title：Journal of clinical microbiology

authors： Schwan TG,Piesman J

更新日期：2000-01-01 00:00:00

abstract：:A total of 151 Escherichia coli strains resistant to cefotaxime and ceftazidime were isolated during a prospective surveillance study. These strains were characterized by clinical, microbiological, and molecular analyses and were distributed into four clusters of 103, 11, 6, and 5 isolates, along with 25 unrelated str...

journal_title：Journal of clinical microbiology

authors： Oteo J,Navarro C,Cercenado E,Delgado-Iribarren A,Wilhelmi I,Orden B,García C,Miguelañez S,Pérez-Vázquez M,García-Cobos S,Aracil B,Bautista V,Campos J

更新日期：2006-07-01 00:00:00

abstract：:A large pool of refined endotoxin was prepared from Escherichia coli O113 by extraction with hot acqueous phenol. It was characterized chemically and biologically and will be available for a reference standard designated as reference endotoxin EC. ...

journal_title：Journal of clinical microbiology

authors： Rudbach JA,Akiya FI,Elin RJ,Hochstein HD,Luoma MK,Milner EC,Milner KC,Thomas KR

更新日期：1976-01-01 00:00:00

abstract：:From late 2003 to the end of 2005, the Health Protection Agency's national reference laboratories received approximately 1,600 referrals of Acinetobacter spp., including 419 and 58 examples, respectively, of two carbapenem-resistant Acinetobacter baumannii lineages, designated OXA-23 clones 1 and 2. Representatives of...

journal_title：Journal of clinical microbiology

authors： Coelho JM,Turton JF,Kaufmann ME,Glover J,Woodford N,Warner M,Palepou MF,Pike R,Pitt TL,Patel BC,Livermore DM

更新日期：2006-10-01 00:00:00

abstract：:RotaTeq (RV5) is a widely used live attenuated pentavalent rotavirus (RV) vaccine. Although fecal shedding of RV vaccine strains persists for long time periods, it is unclear how each vaccine strain replicates in intestinal tissue and is excreted in stool. To examine this issue, we established RV5 genotype-specific re...

journal_title：Journal of clinical microbiology

authors： Higashimoto Y,Ihira M,Miyazaki Y,Kuboshiki A,Yoshinaga S,Hiramatsu H,Suzuki R,Miyata M,Miura H,Komoto S,Yukitake J,Taniguchi K,Kawamura Y,Yoshikawa T

更新日期：2018-05-25 00:00:00

abstract：:We describe here the use of an immunomagnetic separation enrichment process coupled with a modified real-time cellular analysis (RTCA) system (RTCA version 2) for the detection of C. difficile toxin (CDT) in stool. The limit of CDT detection by RTCA version 2 was 0.12 ng/ml. Among the consecutively collected 401 diarr...

journal_title：Journal of clinical microbiology

authors： Huang B,Jin D,Zhang J,Sun JY,Wang X,Stiles J,Xu X,Kamboj M,Babady NE,Tang YW

更新日期：2014-04-01 00:00:00

abstract：:In Brazil, Salmonella enterica serovar Infantis resistant to various antimicrobials, including cephalosporins, has been identified as an etiological agent of severe gastroenteritis in hospitalized children since 1994. In this study, 35 serovar Infantis strains, isolated from children admitted to four different Rio de ...

journal_title：Journal of clinical microbiology

authors： Fonseca EL,Mykytczuk OL,Asensi MD,Reis EM,Ferraz LR,Paula FL,Ng LK,Rodrigues DP

更新日期：2006-08-01 00:00:00

abstract：:A total of 322 single and paired serum samples from children (newborn to 10 years old), young adults (18 to 20 years old), pregnant women and their cord serum samples, and elderly people (more than 70 years old) was tested for antibodies to enteric adenovirus types 40 and 41 by neutralization test. Serum samples were ...

journal_title：Journal of clinical microbiology

authors： Shinozaki T,Araki K,Ushijima H,Fujii R

更新日期：1987-09-01 00:00:00

abstract：:Parameters like zone reading, inoculum density, and plate streaking influence the precision and accuracy of disk diffusion antibiotic susceptibility testing (AST). While improved reading precision has been demonstrated using automated imaging systems, standardization of the inoculum and of plate streaking have not bee...

journal_title：Journal of clinical microbiology

authors： Hombach M,Maurer FP,Pfiffner T,Böttger EC,Furrer R

更新日期：2015-12-01 00:00:00

abstract：:Tissue cultures established from brain tissues of six patients with various chronic, degenerative diseases did not show evidence of viral agents. Sucrose gradient analysis of radioactively labeled culture fluid from two patients failed to reveal the presence of any incomplete or defective virus. Extracellular fluids f...

journal_title：Journal of clinical microbiology

authors： Lennette ET

更新日期：1975-05-01 00:00:00

abstract：:A total of 1,013 isolates of Enterobacteriaceae were identified in parallel by the Repliscan (Cathra International, Ontario, Canada) and API 20E (Analytab Products, Plainview, N.Y.) systems. There was a 62% agreement at the genus level between the two systems. Of the 38% discrepant results, Repliscan classified 22% as...

journal_title：Journal of clinical microbiology

authors： Woolfrey BF,Quall CO,Fox JM

更新日期：1981-01-01 00:00:00

abstract：:A rapid method for the detection of acetylneuraminyl hydrolase, EC 3.2.1.18 (sialidase or neuraminidase), was developed by using 2'-(4-methylumbelliferyl)alpha-D-N-acetylneuraminic acid as substrate in a filter paper spot test. The method was compared to conventional assays that use 2'-(4-methylumbelliferyl)alpha-D-N-...

journal_title：Journal of clinical microbiology

authors： Moncla BJ,Braham P

更新日期：1989-01-01 00:00:00

abstract：:Streptococcus pneumoniae is both a commensal and a major pathogen that causes invasive disease in people of all ages. The introduction of serotype-specific pneumococcal vaccines has reduced the burden of disease but has also led to replacement with new strains; thus, serotyping remains important for vaccine-related di...

journal_title：Journal of clinical microbiology

authors： Pholwat S,Sakai F,Turner P,Vidal JE,Houpt ER

更新日期：2016-07-01 00:00:00

abstract：:Viral load testing for cytomegalovirus (CMV) has become the standard for the diagnosis of infection and monitoring of therapy at many transplant centers. However, no viral load test has been approved by the FDA. Therefore, many laboratories rely on laboratory-developed assays. This study evaluated the performance char...

journal_title：Journal of clinical microbiology

authors： Caliendo AM,Ingersoll J,Fox-Canale AM,Pargman S,Bythwood T,Hayden MK,Bremer JW,Lurain NS

更新日期：2007-06-01 00:00:00

abstract：:Surveillance methods that measure St. Louis encephalitis (SLE) virus activity in nature may provide forewarning of its epidemic occurrence in humans. An antigen capture enzyme immunoassay was developed to detect SLE virus in infected mosquitoes. The assay detected purified SLE viral antigen at a concentration of 62 pg...

journal_title：Journal of clinical microbiology

authors： Tsai TF,Bolin RA,Montoya M,Bailey RE,Francy DB,Jozan M,Roehrig JT

更新日期：1987-02-01 00:00:00

abstract：:The name Psychrobacter immobilis recently has been proposed for a group of chiefly psychrotrophic, aerobic, gram-negative, nonmotile, oxidase-positive coccobacilli commonly found associated with fish, processed meat, and poultry products. This article reports an ocular infection in a 12-day-old newborn who acquired th...

journal_title：Journal of clinical microbiology

authors： Gini GA

更新日期：1990-02-01 00:00:00

abstract：:In a recent genotypic survey of beta-lactam-resistant pneumococci recovered in different areas of United States during 1997, eight clonal types that each represented 3 to 40 isolates accounted for 134 of 144 isolates (G. Gherardi, C. Whitney, R. Facklam, and B. Beall, J. Infect. Dis. 181:216-229, 2000). We determined ...

journal_title：Journal of clinical microbiology

authors： Beall B,Gherardi G,Facklam RR,Hollingshead SK

更新日期：2000-10-01 00:00:00