Emergence of a new swine H3N2 and pandemic (H1N1) 2009 influenza A virus reassortant in two Canadian animal populations, mink and swine.


:A swine H3N2 (swH3N2) and pandemic (H1N1) 2009 (pH1N1) influenza A virus reassortant (swH3N2/pH1N1) was detected in Canadian swine at the end of 2010. Simultaneously, a similar virus was also detected in Canadian mink based on partial viral genome sequencing. The origin of the new swH3N2/pH1N1 viral genes was related to the North American swH3N2 triple-reassortant cluster IV (for hemagglutinin [HA] and neuraminidase [NA] genes) and to pH1N1 for all the other genes (M, NP, NS, PB1, PB2, and PA). Data indicate that the swH3N2/pH1N1 virus can be found in several pigs that are housed at different locations.


J Clin Microbiol


Tremblay D,Allard V,Doyon JF,Bellehumeur C,Spearman JG,Harel J,Gagnon CA




Has Abstract


2011-12-01 00:00:00














  • Chlamydia trachomatis serovar distribution and Neisseria gonorrhoeae coinfection in male patients with urethritis in Greece.

    abstract::The distribution of Chlamydia trachomatis serovars and Neisseria gonorrhoeae coinfection was studied in a group of 100 C. trachomatis-positive males with urethritis in Greece. The serovar distribution revealed that apart from the predominant worldwide types E and F, the relatively uncommon type G is also prevalent. Go...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Papadogeorgakis H,Pittaras TE,Papaparaskevas J,Pitiriga V,Katsambas A,Tsakris A

    更新日期:2010-06-01 00:00:00

  • Study of restriction fragment length polymorphism analysis and spoligotyping for epidemiological investigation of Mycobacterium bovis infection.

    abstract::Restriction fragment length polymorphism (RFLP) analysis with probes derived from the insertion element IS6110, the direct repeat sequence, and the polymorphic GC-rich sequence (PGRS) and a PCR-based typing method called spacer oligonucleotide typing (spoligotyping) were used to strain type Mycobacterium bovis isolate...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Costello E,O'Grady D,Flynn O,O'Brien R,Rogers M,Quigley F,Egan J,Griffin J

    更新日期:1999-10-01 00:00:00

  • Comparison of agar dilution, broth microdilution, E-test, disk diffusion, and automated Vitek methods for testing susceptibilities of Enterococcus spp. to vancomycin.

    abstract::An evaluation was undertaken to determine the optimal method for testing the susceptibilities of 100 clinical isolates and two reference strains of Enterococcus spp. to vancomycin in vitro. Six testing methods were studied by using the following media and incubation times: agar screen with the Synergy Quad Plate (Reme...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Kohner PC,Patel R,Uhl JR,Garin KM,Hopkins MK,Wegener LT,Cockerill FR 3rd

    更新日期:1997-12-01 00:00:00

  • Persistence of immunoglobulin G and immunoglobulin M antibodies after postnatal rubella infection determined by solid-phase radioimmunoassay.

    abstract::The appearance and persistence of immunoglobulin M (IgM) and IgG antibodies in postnatal rubella infections were studied by employing a solid-phase radioimmunoassay test. Altogether, 222 serial serum specimens from 51 patients with acute rubella infection were tested. Both IgG and IgM antibodies developed rapidly and ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Meurman OH

    更新日期:1978-01-01 00:00:00

  • Molecular typing of Borrelia burgdorferi sensu lato by randomly amplified polymorphic DNA fingerprinting analysis.

    abstract::To study whether pathogenic clusters of Borrelia burgdorferi sensu lato strains occur, we typed 136 isolates, cultured from specimens from patients (n = 49) with various clinical entities and from ticks (n = 83) or dogs (n = 4) from different geographic regions, by randomly amplified polymorphic DNA (RAPD) fingerprint...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Wang G,van Dam AP,Spanjaard L,Dankert J

    更新日期:1998-03-01 00:00:00

  • Rapid detection and identification of Candida albicans and Torulopsis (Candida) glabrata in clinical specimens by species-specific nested PCR amplification of a cytochrome P-450 lanosterol-alpha-demethylase (L1A1) gene fragment.

    abstract::PCR of a Candida albicans cytochrome P-450 lanosterol-alpha-demethylase (P450-L1A1) gene segment is a rapid and sensitive method of detection in clinical specimens. This enzyme is a target for azole antifungal action. In order to directly detect and identify the clinically most important species of Candida, we cloned ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Burgener-Kairuz P,Zuber JP,Jaunin P,Buchman TG,Bille J,Rossier M

    更新日期:1994-08-01 00:00:00

  • Comparison of the yields of blood cultures using serum or plasma from patients with early Lyme disease.

    abstract::In an initial experiment, culture-grown Borrelia burgdorferi was added to freshly collected uninfected human blood. This in vitro study demonstrated that more spirochetes were distributed into the plasma than into the serum fraction. In a subsequent clinical study, B. burgdorferi was recovered from plasma cultures of ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Wormser GP,Bittker S,Cooper D,Nowakowski J,Nadelman RB,Pavia C

    更新日期:2000-04-01 00:00:00

  • Treponema-specific tests for serodiagnosis of syphilis: comparative evaluation of seven assays.

    abstract::The diagnosis of syphilis is challenging and often relies on serologic tests to detect treponemal or nontreponemal antibodies. Recently, the Centers for Disease Control and Prevention and the Association of Public Health Laboratories proposed an update to the syphilis serology testing algorithm, in which serum samples...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Binnicker MJ,Jespersen DJ,Rollins LO

    更新日期:2011-04-01 00:00:00

  • Dissemination of Salmonella enterica subsp. enterica Serovar Typhimurium var. Copenhagen clonal types through a contract heifer-raising operation.

    abstract::Salmonella enterica subsp. enterica serovar Typhimurium var. Copenhagen isolates from a heifer-raising operation and from 11 dairy herds that had their calves contracted to the heifer-raising operation were examined for their phenotypic and genotypic characteristics. Results of the study showed that the heifer-raising...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Hegde NV,Cook ML,Wolfgang DR,Love BC,Maddox CC,Jayarao BM

    更新日期:2005-08-01 00:00:00

  • Evaluation of the WASPLab Segregation Software To Automatically Analyze Urine Cultures Using Routine Blood and MacConkey Agars.

    abstract::Automation of the clinical microbiology laboratory has become more prominent as laboratories face higher specimen volumes and understaffing and are becoming more consolidated. One recent advancement is the use of digital image analysis to rapidly distinguish between chromogenic growth for screening bacterial cultures....

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Faron ML,Buchan BW,Relich RF,Clark J,Ledeboer NA

    更新日期:2020-03-25 00:00:00

  • Evaluation of MicroScan rapid gram-positive panels for detection of oxacillin-resistant staphylococci.

    abstract::The ability of MicroScan rapid panels (Baxter MicroScan, West Sacramento, Calif.) to detect oxacillin resistance in 92 clinical isolates of Staphylococcus aureus and 103 coagulase-negative staphylococci was evaluated by comparing results with those of MicroScan 24-h MIC panels and, to resolve discrepancies, oxacillin ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Woods GL,LaTemple D,Cruz C

    更新日期:1994-04-01 00:00:00

  • Evaluation of a rapid PCR assay for diagnosis of meningococcal meningitis.

    abstract::We compared the results of Gram staining and culture of cerebrospinal fluid to results obtained with a rapid PCR assay for the diagnosis of meningococcal meningitis in 281 cases of suspected bacterial meningitis. PCR had a sensitivity of 97% compared to a sensitivity of 55% for culture, and the PCR specificity was 99....

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Richardson DC,Louie L,Louie M,Simor AE

    更新日期:2003-08-01 00:00:00

  • Use of several inducer and substrate antibiotic combinations in a disk approximation assay format to screen for AmpC induction in patient isolates of Pseudomonas aeruginosa, Enterobacter spp., Citrobacter spp., and Serratia spp.

    abstract::Two-hundred consecutive, single patient isolates of Enterobacter spp., Serratia spp., Citrobacter spp., and Pseudomonas aeruginosa were evaluated for AmpC production using a variety of inducer-substrate antibiotic combinations in a disk approximation format. The combinations examined included cefoxitin-piperacillin, i...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Dunne WM Jr,Hardin DJ

    更新日期:2005-12-01 00:00:00

  • Bacteriology of dental abscesses of endodontic origin.

    abstract::Aspirates have been cultured from 10 dental abscesses of endodontic origin, all of which had penetrated beyond the bony alveolus to produce fluctuant swelling. Sampling was by syringe aspiration. Strict anaerobic techniques, including the use of an anaerobic chamber, were used for serial dilution and plating. Randomly...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Williams BL,McCann GF,Schoenknecht FD

    更新日期:1983-10-01 00:00:00

  • Sensitive and specific serodiagnosis of Lyme disease by enzyme-linked immunosorbent assay with a peptide based on an immunodominant conserved region of Borrelia burgdorferi vlsE.

    abstract::VlsE, the variable surface antigen of Borrelia burgdorferi, contains an immunodominant conserved region named IR(6). In the present study, the diagnostic performance of a peptide enzyme-linked immunosorbent assay (ELISA) based on a 26-mer synthetic peptide (C(6)) with the IR(6) sequence was explored. Sensitivity was a...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Liang FT,Steere AC,Marques AR,Johnson BJ,Miller JN,Philipp MT

    更新日期:1999-12-01 00:00:00

  • Similarity of bacterial populations in saliva from African-American mother-child dyads.

    abstract::Using PCR-based denaturing gradient gel electrophoresis analyses of oral bacterial samples in 20 mother-child dyads, this study demonstrated a high degree of similarity of bacterial compositions between the mothers and their children; the two may share as much as 94% of their oral bacterial spectra, including cariogen...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Li Y,Ismail AI,Ge Y,Tellez M,Sohn W

    更新日期:2007-09-01 00:00:00

  • Development of a LightCycler PCR assay for detection and quantification of Aspergillus fumigatus DNA in clinical samples from neutropenic patients.

    abstract::The increasing incidence of invasive aspergillosis, a life-threatening infection in immunocompromised patients, emphasizes the need to improve the diagnostic tools for this disease. We established a LightCycler-based real-time PCR assay to detect and quantify rapidly, specifically, and sensitively Aspergillus fumigatu...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Spiess B,Buchheidt D,Baust C,Skladny H,Seifarth W,Zeilfelder U,Leib-Mösch C,Mörz H,Hehlmann R

    更新日期:2003-05-01 00:00:00

  • Classification of rotavirus into G and P types with specimens from children with acute diarrhea in New Delhi, India.

    abstract::Sixty rotavirus-positive stool specimens from children with diarrhea were classified into G and P genotypes. G typing was done by PCR and then by hybridization with G type-specific (G1 to G4) oligonucleotide probes, whereas nested PCR was performed for P typing. Thirty-nine samples could be classified into both G and ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Husain M,Seth P,Dar L,Broor S

    更新日期:1996-06-01 00:00:00

  • Peritonitis involving a Capnocytophaga sp. in a patient undergoing continuous ambulatory peritoneal dialysis.

    abstract::Capnocytophaga is a genus of gram-negative rods involved mainly in bacteremia in immunocompromised hosts. Here we report a case of Capnocytophaga peritonitis in a patient undergoing continuous ambulatory peritoneal dialysis. The disease appeared as a superinfection during antimicrobial therapy of a previous episode of...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Esteban J,Albalate M,Caramelo C,Reyero A,Carriazo MA,Hernandez J,Ortiz A,Soriano F

    更新日期:1995-09-01 00:00:00

  • Serotypic differentiation of rotaviruses in field samples from diarrheic pigs by using nucleic acid probes specific for porcine VP4 and human and porcine VP7 genes.

    abstract::Of 216 fecal and intestinal samples collected from nursing or weaned diarrheic pigs in the United States and Canada, 57 were identified as group A rotavirus positive by RNA electrophoresis and silver staining. Fifty-seven and 52 rotavirus-positive samples were analyzed by hybridization with Gottfried and OSU PCR-deriv...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Rosen BI,Parwani AV,Lopez S,Flores J,Saif LJ

    更新日期:1994-02-01 00:00:00

  • Outbreak of vancomycin-resistant Enterococcus faecium of the phenotype VanB in a hospital in Warsaw, Poland: probable transmission of the resistance determinants into an endemic vancomycin-susceptible strain.

    abstract::The first outbreak caused by vancomycin-resistant enterococci of the VanB phenotype in Poland was analyzed. It occurred in a single ward of a Warsaw hospital which is a specialized center for the treatment of hematological disorders. Between July 1999 and February 2000, 11 patients in the ward were found to be infecte...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Kawalec M,Gniadkowski M,Zaleska M,Ozorowski T,Konopka L,Hryniewicz W

    更新日期:2001-05-01 00:00:00

  • Molecular subtyping of Neisseria meningitidis serogroup B: comparison of five methods.

    abstract::In order to compare methods for subtyping Neisseria meningitidis serogroup B isolates, 96 isolates obtained from various locations in the United States and northwestern Europe were subtyped by five methods: monoclonal antibody (MAb)-based serotyping and serosubtyping, DNA macrorestriction analysis by pulsed-field gel ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Swaminathan B,Matar GM,Reeves MW,Graves LM,Ajello G,Bibb WF,Helsel LO,Morales M,Dronavalli H,el-Swify M,DeWitt W,Hunter SB

    更新日期:1996-06-01 00:00:00

  • Identification and broad dissemination of the CTX-M-14 beta-lactamase in different Escherichia coli strains in the northwest area of Spain.

    abstract::During the course of a molecular epidemiology study of mechanisms of antibiotic resistance in the area served by our hospital (516,000 inhabitants), we isolated the gene encoding CTX-M-14 beta-lactamase. Thirty clinical strains (27 Escherichia coli and 3 Klebsiella pneumoniae isolates) with a phenotype of extended-spe...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Bou G,Cartelle M,Tomas M,Canle D,Molina F,Moure R,Eiros JM,Guerrero A

    更新日期:2002-11-01 00:00:00

  • Staphylococcus aureus capsular types and antibody response to lung infection in patients with cystic fibrosis.

    abstract::Chronic respiratory tract infections caused by Staphylococcus aureus are common in patients with cystic fibrosis (CF). Recently, it was shown in a few CF patients that S. aureus isolates produce capsular polysaccharides (CPs). However, it is not known whether this is a common feature and whether an immune response to ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Albus A,Fournier JM,Wolz C,Boutonnier A,Ranke M,Høiby N,Hochkeppel H,Döring G

    更新日期:1988-12-01 00:00:00

  • Mycobacterium avium complex, an emerging pathogen in Massachusetts.

    abstract::We report a study of 1,953 patients whose laboratory records from 1972 through 1983 at the Massachusetts Mycobacteria Reference Laboratory indicated the isolation of Mycobacterium avium complex (MAC) organisms. At least one clinical specimen from each patient during this period exhibited the organism. The incidence of...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: du Moulin GC,Sherman IH,Hoaglin DC,Stottmeier KD

    更新日期:1985-07-01 00:00:00

  • Epidemiology of severe Streptococcus pyogenes disease in Europe.

    abstract::The past 2 decades have brought worrying increases in severe Streptococcus pyogenes diseases globally. To investigate and compare the epidemiological patterns of these diseases within Europe, data were collected through a European Union FP-5-funded program (Strep-EURO). Prospective population-based surveillance of sev...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Lamagni TL,Darenberg J,Luca-Harari B,Siljander T,Efstratiou A,Henriques-Normark B,Vuopio-Varkila J,Bouvet A,Creti R,Ekelund K,Koliou M,Reinert RR,Stathi A,Strakova L,Ungureanu V,Schalén C,Strep-EURO Study Group.,Jasir A

    更新日期:2008-07-01 00:00:00

  • Successful Combination of Nucleic Acid Amplification Test Diagnostics and Targeted Deferred Neisseria gonorrhoeae Culture.

    abstract::Nucleic acid amplification tests (NAATs) are recommended for the diagnosis of N. gonorrhoeae infections because of their superior sensitivity. Increasing NAAT use causes a decline in crucial antimicrobial resistance (AMR) surveillance data, which rely on culture. We analyzed the suitability of the ESwab system for NAA...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Wind CM,de Vries HJ,Schim van der Loeff MF,Unemo M,van Dam AP

    更新日期:2015-06-01 00:00:00

  • Intraocular detection of Bartonella henselae in a patient with HLA-B27 uveitis.

    abstract::Bartonella henselae uveitis was diagnosed in a 40-year-old woman with underlying HLA-B27 uveitis on the basis of immunodetection and molecular detection of the organism, a Marseilles genogroup and a CAL-1 genotype strain, in the vitreous fluid. This case illustrates that B. henselae should be included in the different...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Drancourt M,Bodaghi B,Lepidi H,Le Hoang P,Raoult D

    更新日期:2004-04-01 00:00:00

  • Rapid identification of bacteria by PCR-single-strand conformation polymorphism.

    abstract::A new molecular biological approach for the identification of bacteria is described. This approach employs PCR of bacterial cell lysates with conserved primers located in the 16S rRNA sequence flanking a variable region, and analysis of the amplified product was based on the principle of single-strand conformation pol...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Widjojoatmodjo MN,Fluit AC,Verhoef J

    更新日期:1994-12-01 00:00:00

  • Cloned polynucleotide and synthetic oligonucleotide probes used in colony hybridization are equally efficient in the identification of enterotoxigenic Escherichia coli.

    abstract::Restriction endonuclease-generated polynucleotide and synthetically produced oligonucleotide gene probes used in colony hybridization assays proved to be efficient for the detection and differentiation of enterotoxigenic Escherichia coli. To compare their relative efficiencies, these two sets of probes were radiolabel...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Sommerfelt H,Kalland KH,Raj P,Moseley SL,Bhan MK,Bjorvatn B

    更新日期:1988-11-01 00:00:00