Emergence of a new swine H3N2 and pandemic (H1N1) 2009 influenza A virus reassortant in two Canadian animal populations, mink and swine.

abstract：:Autopsy specimens of lung tissues from 15 patients that contracted legionellosis during the 1976 Philadelphia outbreak of Legionnaires disease were examined for the presence of Legionella organisms and soluble antigens by indirect fluorescent-antibody (IFA) testing and by an enzyme-linked immunosorbent assay (ELISA) w...

journal_title：Journal of clinical microbiology

authors： Brown SL,Bibb WF,McKinney RM

更新日期：1985-01-01 00:00:00

abstract：:A simple new agar medium containing L-canavanine, glycine, and bromthymol blue was found to give a clearer and more accurate distinction between serotype A or D (Cryptococcus neoformans var. neoformans) and serotype B or C (C. neoformans var. gattii) than creatinine-dextrose-bromthymol blue or glycine-cycloheximide-ph...

journal_title：Journal of clinical microbiology

authors： Kwon-Chung KJ,Polacheck I,Bennett JE

更新日期：1982-03-01 00:00:00

abstract：:Acute and convalescent-phase sera obtained from 10 patients infected with a Japanese strain of spotted fever group (SFG) rickettsia were tested by the indirect immunoperoxidase test, the Weil-Felix test, an enzyme-linked immunosorbent assay (ELISA), and immunoblotting. By the Weil-Felix test, the reactivity of these s...

journal_title：Journal of clinical microbiology

authors： Amano K,Hatakeyama H,Okuta M,Suto T,Mahara F

更新日期：1992-09-01 00:00:00

abstract：:Recent reports suggest an association between Chlamydia pneumoniae and Helicobacter pylori bacteria and atherosclerosis. We studied 51 patients (mean age, 68.3 years) who underwent abdominal aortic aneurysm surgery. For each patient we performed a microimmunofluorescence test for immunoglobulin G (IgG), IgA, and IgM a...

journal_title：Journal of clinical microbiology

authors： Blasi F,Denti F,Erba M,Cosentini R,Raccanelli R,Rinaldi A,Fagetti L,Esposito G,Ruberti U,Allegra L

更新日期：1996-11-01 00:00:00

abstract：:The moderately repetitive sequence Ca3 was used to fingerprint strains of Candida albicans isolated from vulvovaginal infections of 10 women and strains isolated from their male partners. The Dendron software package was then used to compare the DNA fingerprints of these strains with those of vaginal commensals from w...

journal_title：Journal of clinical microbiology

authors： Schmid J,Rotman M,Reed B,Pierson CL,Soll DR

更新日期：1993-01-01 00:00:00

abstract：:Pyridine-2-azo-p-dimethylanaline cephalosporin (PADAC), a chromogenic reagent which is purple and changes to yellow upon cleavage of its beta-lactam ring, was evaluated in comparison with other chromogenic cephalosporins. PADAC exhibited little antimicrobial activity against gram-negative bacteria, but did have good a...

journal_title：Journal of clinical microbiology

authors： Jones RN,Wilson HW,Novick WJ Jr

更新日期：1982-04-01 00:00:00

abstract：:A new method has been developed for demonstration of heat-labile (LT) enterotoxin produced by Escherichia coli. This method is based upon the release of LT from bacteria grown directly onto agar plates which have been coated with ganglioside GM1. Toxin bound to the GM1 solid state is subsequently demonstrated by means...

journal_title：Journal of clinical microbiology

authors： Czerkinsky CC,Svennerholm AM

更新日期：1983-06-01 00:00:00

abstract：:Evaluations of screening tests for bacteriuria have traditionally compared the test results with those of quantitative urine cultures. However, many patients with symptomatic urinary tract infections can have less than 10(5) CFU/ml in their urine. Therefore, the results of urine culture and three screening tests (Bac-...

journal_title：Journal of clinical microbiology

authors： Murray PR,Smith TB,McKinney TC Jr

更新日期：1987-03-01 00:00:00

abstract：:Pluronic polyols markedly improved the growth of two human lymphocyte cell lines when added to the growth medium in concentrations of 0.05 to 0.1%. The results of the current studies suggest that, in addition to the protective effect of polyols against mechanical damage of mammalian cells in submerged cultures, the pl...

journal_title：Journal of clinical microbiology

authors： Mizrahi A

更新日期：1975-07-01 00:00:00

abstract：:Murine monoclonal antibodies (MAbs) against herpes simplex virus type 1 and 2 (HSV-1 and -2, respectively) nuclear antigens were used to identify cells infected with HSV-1 or -2 by indirect immunofluorescence in conjunction with flow cytometry after virus amplification of MRC-5 cell monolayers. The results indicate th...

journal_title：Journal of clinical microbiology

authors： McSharry JJ,Costantino R,McSharry MB,Venezia RA,Lehman JM

更新日期：1990-08-01 00:00:00

abstract：:Lipovitellin-salt-mannitol (LSM) plate medium was examined for its ability to directly isolate, recover, and presumptively identify Staphylococcus aureus from 418 clinical specimens. The criteria for medium evaluation included colony morphology reactions, selectivity, and ease of isolation. For 298 specimens used for ...

journal_title：Journal of clinical microbiology

authors： Merlino J,Gill R,Robertson GJ

更新日期：1996-12-01 00:00:00

abstract：:Quantitation of hepatitis C virus (HCV) RNA in plasma and serum samples is a costly procedure in both time and reagents. Additionally, cell-associated viral RNA may not be detected. This study evaluated the accuracy of HCV RNA quantitation in small-volume whole-blood (WB) samples, which would be appropriate for point-...

journal_title：Journal of clinical microbiology

authors： Bruns T,Steinmetzer K,Ermantraut E,Stallmach A

更新日期：2009-10-01 00:00:00

abstract：:During a 15-month period, two methods for detection of herpes simplex virus (HSV) in 699 clinical specimens were compared: (i) 24-well-plate centrifugation (24WPC) with MRC-5 cells and staining with type-specific monoclonal antibodies (Syva Co., Palo Alto, Calif.) after incubation for 16 to 18 h and (ii) conventional ...

journal_title：Journal of clinical microbiology

authors： Woods GL,Mills RD

更新日期：1988-03-01 00:00:00

abstract：:It is unclear whether patients colonized with methicillin-resistant Staphylococcus aureus (MRSA) continue to harbor the same genotype during hospital readmissions. We characterized 140 MRSA strains isolated from 33 persistent MRSA carriers with hospital readmissions. Nearly half of the patients continued to harbor the...

journal_title：Journal of clinical microbiology

authors： Eghbaria S,Schnitser K,Danial-Farran N,Keness Y,Bisharat N

更新日期：2014-04-01 00:00:00

abstract：:Spontaneously occurring urinary tract infection (UTI) in dogs was exploited as an experiment of nature to gain insights into UTI pathogenesis in humans. Concurrent urinary and rectal Escherichia coli isolates from 37 dogs with UTI were compared with respect to phylogenetic background, O antigens, and extended virulenc...

journal_title：Journal of clinical microbiology

authors： Johnson JR,Kaster N,Kuskowski MA,Ling GV

更新日期：2003-01-01 00:00:00

abstract：:Of 100 strains of enterococci, 83% reduced litmus milk within 4 h. None of the 100 non-enterococcal streptococci tested were positive. ...

journal_title：Journal of clinical microbiology

authors： Schierl EA,Blazevic DJ

更新日期：1981-08-01 00:00:00

abstract：:The extent and importance of genotype heterogeneity of Pneumocystis carinii f. sp. hominis within lungs have not previously been investigated. Two hundred forty PCR clones obtained from respiratory specimens and lung segments from three patients with fatal P. carinii pneumonia were investigated to detect genetic diver...

journal_title：Journal of clinical microbiology

authors： Helweg-Larsen J,Lundgren B,Lundgren JD

更新日期：2001-10-01 00:00:00

abstract：:A serum immunoglobulin G enzyme immunoassay (EIA) for Helicobacter pylori antibodies already in use in adults was evaluated with 99 pediatric serum samples to determine its usefulness for the study of H. pylori disease in children. The reference method used was either the (13)C-urea breath test or a biopsy culture of ...

journal_title：Journal of clinical microbiology

authors： Sunnerstam B,Kjerstadius T,Jansson L,Giesecke J,Bergström M,Ejderhamn J

更新日期：1999-10-01 00:00:00

abstract：:Analysis of 177 methicillin-resistant Staphylococcus aureus (MRSA) strains for the presence of egc and tst revealed that 60 strains carried at least one of the tested genes. MRSA strains were classified by pulsed-field gel electrophoresis into four clones belonging to agr groups I and III. Toxin genotypes were related...

journal_title：Journal of clinical microbiology

authors： Chini V,Dimitracopoulos G,Spiliopoulou I

更新日期：2006-05-01 00:00:00

abstract：:Between September 1995 and August 1998, the incidence and diversity of the main human rotavirus genotypes (G1, G2, G3, and G4 and P[8], P[4], P[6], and P[9]) among Irish children were determined by using established and adapted reverse transcriptase PCR-based genotyping methods. From a total of 193 rotavirus-positive ...

journal_title：Journal of clinical microbiology

authors： O'Mahony J,Foley B,Morgan S,Morgan JG,Hill C

更新日期：1999-06-01 00:00:00

abstract：:The TRUGENE HIV-1 Genotyping Kit and OpenGene DNA Sequencing System are designed to sequence the protease (PR)- and reverse transcriptase (RT)-coding regions of human immunodeficiency virus type 1 (HIV-1) pol. Studies were undertaken to determine the accuracy of this assay system in detecting resistance-associated mut...

journal_title：Journal of clinical microbiology

authors： Kuritzkes DR,Grant RM,Feorino P,Griswold M,Hoover M,Young R,Day S,Lloyd RM Jr,Reid C,Morgan GF,Winslow DL

更新日期：2003-04-01 00:00:00

abstract：:Fingerprinting by randomly amplified polymorphic DNA (RAPD) analysis was used to differentiate Scedosporium prolificans isolates. A total of 59 arbitrary primers were screened with six unrelated S. prolificans isolates, and a panel of 12 primers was selected. The 12 primers were then used to detect DNA polymorphisms a...

journal_title：Journal of clinical microbiology

authors： San Millán R,Quindós G,Garaizar J,Salesa R,Guarro J,Pontón J

更新日期：1997-09-01 00:00:00

abstract：:A singleplex PCR assay using a single primer pair targeting the putative sugar transporter gene was developed here to distinguish Cryptococcus neoformans var. grubii, Cryptococcus neoformans var. neoformans, and Cryptococcus gattii according to the distinct size of the amplicon. The interspecies and intravarietal hybr...

journal_title：Journal of clinical microbiology

authors： Feng X,Fu X,Ling B,Wang L,Liao W,Yao Z

更新日期：2013-06-01 00:00:00

abstract：:We compared whole blood dried on filter paper to the standard assay with frozen cell-free plasma for use in the quantitation of the human immunodeficiency virus RNA load in blood. RNA values from filter paper, corrected for the hematocrit, gave results comparable to those of the standard assay in terms of sensitivity ...

journal_title：Journal of clinical microbiology

authors： Fiscus SA,Brambilla D,Grosso L,Schock J,Cronin M

更新日期：1998-01-01 00:00:00

abstract：:In this report, we present a case of rhino-orbital mucormycosis in a 57-year-old female with poorly controlled diabetes mellitus. The only mold cultured at 25°C, 37°C, and 40°C from a specimen of the nasal crust was identified phenotypically and independently using nuclear ribosomal DNA sequence data as Thamnostylum l...

journal_title：Journal of clinical microbiology

authors： Xess I,Mohapatra S,Shivaprakash MR,Chakrabarti A,Benny GL,O'Donnell K,Padhye AA

更新日期：2012-04-01 00:00:00

abstract：:We report a case in which fecal microbiota transplantation (FMT) utilized for relapsing Clostridium difficile colitis successfully eradicated colonization with several multidrug-resistant organisms (MDROs). FMT may have an additive benefit of reducing MDRO carriage and should be further investigated as a potential mea...

journal_title：Journal of clinical microbiology

authors： Crum-Cianflone NF,Sullivan E,Ballon-Landa G

更新日期：2015-06-01 00:00:00

abstract：:Methicillin-resistant Staphylococcus aureus (MRSA) of sequence type 398 (ST398) has frequently been detected in pigs and pig handlers. However, in Malaysia, sampling 360 pigs and 90 pig handlers from 30 farms identified novel ST9-spa type t4358-staphylococcal cassette chromosome mec type V MRSA strains that were found...

journal_title：Journal of clinical microbiology

authors： Neela V,Mohd Zafrul A,Mariana NS,van Belkum A,Liew YK,Rad EG

更新日期：2009-12-01 00:00:00

abstract：:A simple solid-phase enzyme-linked immunosorbent assay procedure for the detection of human antibodies to Entamoeba histolytica was developed which showed a high degree of correlation with the agar gel diffusion, counterelectrophoresis, and indirect hemagglutination methods, as well as with clinical data. The enzyme-l...

journal_title：Journal of clinical microbiology

authors： Lin TM,Halbert SP,Chiu CT,Zarco R

更新日期：1981-04-01 00:00:00

abstract：:A total of 122 adenovirus type 4 (Ad4) strains isolated from patients with epidemic keratoconjunctivitis at an eye clinic in Sapporo, Japan, from 1985 to 1989 were identified as Ad4a. By the use of HinfI and TaqI restriction endonucleases, these Ad4a strains were classified into 12 subgenome types. During this observa...

journal_title：Journal of clinical microbiology

authors： Itakura S,Aoki K,Sawada H,Ishiguro N,Shinagawa M

更新日期：1991-08-01 00:00:00

abstract：:Sequence-based typing (SBT), analogous to multilocus sequence typing (MLST), is the current "gold standard" typing method for investigation of legionellosis outbreaks caused by Legionella pneumophila However, as common sequence types (STs) cause many infections, some investigations remain unresolved. In this study, va...

journal_title：Journal of clinical microbiology

authors： David S,Mentasti M,Tewolde R,Aslett M,Harris SR,Afshar B,Underwood A,Fry NK,Parkhill J,Harrison TG

更新日期：2016-08-01 00:00:00