Prevalence of ST9 methicillin-resistant Staphylococcus aureus among pigs and pig handlers in Malaysia.

abstract：:We evaluated a new rapid influenza diagnostic test for the pandemic (H1N1) 2009 influenza virus by using real-time reverse transcription-PCR (rRT-PCR) and viral culture. The sensitivities were 68.5% and 64.5%, and the specificities were 98.4% and 97.6%, respectively. This kit should be used with caution, and negative ...

journal_title：Journal of clinical microbiology

authors： Kwon D,Shin K,Kwon M,Oh HB,Kang C,Lee JY

更新日期：2011-01-01 00:00:00

abstract：:Gram-negative, anaerobic bacilli are unusual organisms to be isolated in cases of acute septic arthritis. We report the isolation of Fusobacterium necrophorum from joint aspirate in a case of acute septic arthritis, which presented 3 weeks after the drainage of a dental abscess. ...

journal_title：Journal of clinical microbiology

authors： Sonsale PD,Philipson MR,Bowskill J

更新日期：2004-07-01 00:00:00

abstract：:There is a greatly increased interest in Herpesvirus hominis infections especially those of type 2 associated with genital lesions or neonatal disease. Physicians are eager to confirm the clinical impression with a specific virologic diagnosis such as isolation of the agent and its typing, and type-specific antibody r...

journal_title：Journal of clinical microbiology

authors： Hanna L,Keshishyan H,Jawetz E,Coleman VR

更新日期：1975-03-01 00:00:00

abstract：:The supposedly first outbreak of hepatitis D virus (HDV) infection in Sweden occurred among intravenous drug addicts in the Malmö area in the mid-1970s. Stored sera from this outbreak were used for viral RNA extraction and analysis. By sequence comparisons, the HDV genomes from those Swedish patients fell into two sep...

journal_title：Journal of clinical microbiology

authors： Zhang YY,Hansson BG

更新日期：1996-11-01 00:00:00

abstract：:Multidrug-resistant opportunistic pathogens have become endemic to the veterinary hospital environment. Escherichia coli isolates resistant to 12 antibiotics were isolated from two dogs that were housed in the intensive care unit at The University of Georgia Veterinary Teaching Hospital within 48 h of each other. Revi...

journal_title：Journal of clinical microbiology

authors： Sanchez S,McCrackin Stevenson MA,Hudson CR,Maier M,Buffington T,Dam Q,Maurer JJ

更新日期：2002-10-01 00:00:00

abstract：:Conventional culture techniques are limited in the ability to detect multiple Streptococcus pneumoniae serotypes in nasopharyngeal (NP) secretions. We developed an immunoblot (IB) method with monoclonal antibodies (MAbs) to detect S. pneumoniae and to identify serotypes. NP specimens stored in skim milk-tryptone-gluco...

journal_title：Journal of clinical microbiology

authors： Bronsdon MA,O'Brien KL,Facklam RR,Whitney CG,Schwartz B,Carlone GM

更新日期：2004-04-01 00:00:00

abstract：:The first vector-borne Capnocytophaga canimorsus sepsis case is presented. An immunocompetent male who denied any contact with canines and who worked in a sawmill was bitten on his neck by a large pine weevil (Hylobius abietis L.; Coleoptera: Curculionidae). Bacteriological diagnosis was confirmed by 16S rRNA gene seq...

journal_title：Journal of clinical microbiology

authors： Tuuminen T,Viiri H,Vuorinen S

更新日期：2014-07-01 00:00:00

abstract：:We prospectively studied 156 patients with a diagnosis of community-acquired pneumonia requiring admission. Several respiratory specimens were obtained for the detection of Chlamydia pneumoniae by cell culture and PCR. Three serum samples were obtained from each patient. Serological diagnosis of a C. pneumoniae infect...

journal_title：Journal of clinical microbiology

authors： Verkooyen RP,Willemse D,Hiep-van Casteren SC,Joulandan SA,Snijder RJ,van den Bosch JM,van Helden HP,Peeters MF,Verbrugh HA

更新日期：1998-08-01 00:00:00

abstract：:Sensitivities of DNA extraction methods and PCR methods for Giardia duodenalis were evaluated. A combination of the most sensitive methods, i.e., FTA filter paper and a PCR protocol using RH11/RH4 and GiarF/GiarR primers, showed no significant differences compared to immunofluorescence assay in terms of their sensitiv...

journal_title：Journal of clinical microbiology

authors： Nantavisai K,Mungthin M,Tan-ariya P,Rangsin R,Naaglor T,Leelayoova S

更新日期：2007-02-01 00:00:00

abstract：:In this study we evaluated the ability of three commercial methods, API 20A (Analytab Products, Plainview, N.Y.), Minitek (BBL Microbiology Systems, Cockeysville, Md.), and Anaerobe-Tek (Flow Laboratories, Inc., McLean, Va.), to accurately identify 165 recent clinical and 38 stock isolates of anaerobic bacteria withou...

journal_title：Journal of clinical microbiology

authors： Appelbaum PC,Kaufmann CS,Keifer JC,Venbrux HJ

更新日期：1983-09-01 00:00:00

abstract：:A long-term outbreak of urinary tract-associated multiply resistant Providencia stuartii occurred in a large medical facility that included a 513-bed chronic care unit. The unique characteristics of this outbreak were that from within a single medical facility, P. stuartii with multiple serotypes, biotypes, and antibi...

journal_title：Journal of clinical microbiology

authors： Kocka FE,Srinivasan S,Mowjood M,Kantor HS

更新日期：1980-02-01 00:00:00

abstract：:Clostridium difficile is the bacterium most commonly surmised to cause antimicrobial- and hospital-associated diarrhea in developed countries worldwide, and such infections are thought to be increasing in frequency and severity. A laboratory-based study was carried out to characterize C. difficile strains isolated fro...

journal_title：Journal of clinical microbiology

authors： Martin H,Willey B,Low DE,Staempfli HR,McGeer A,Boerlin P,Mulvey M,Weese JS

更新日期：2008-09-01 00:00:00

abstract：:The clinical observation has been made that there might be an unacceptable number of false-positive enzyme immunoassay (EIA) test results for IgM among persons suspected of having coccidioidomycosis. Patients with a positive result for IgM by EIA are thought to have a diagnosis of acute coccidioidomycosis. However, th...

journal_title：Journal of clinical microbiology

authors： Kuberski T,Herrig J,Pappagianis D

更新日期：2010-06-01 00:00:00

abstract：:The Centers for Disease Control and Prevention recommended confirming positive screening tests for Chlamydia trachomatis when positive predictive values are <90%. It is accepted that less sensitive tests (i.e., culture and immunoassays) should not be used to confirm the results of more sensitive nucleic acid amplifica...

journal_title：Journal of clinical microbiology

authors： Schachter J,Hook EW,Martin DH,Willis D,Fine P,Fuller D,Jordan J,Janda WM,Chernesky M

更新日期：2005-03-01 00:00:00

abstract：:Using the Staphylococcus aureus absorption method, we have modified a commercial radioimmunoassay kit for the determination of total antibody to hepatitis B core antigen so that we can now determine the predominant globulin species of that antibody. One-hundred percent of acute hepatitis B sera had a predominance of i...

journal_title：Journal of clinical microbiology

authors： Schable CA,Maynard JE

更新日期：1982-11-01 00:00:00

abstract：:The recent Zaire Ebola virus (EBOV) outbreak in West Africa illustrates clearly the need for additional studies with humans and animals to elucidate the ecology of Ebola viruses (EBVs). In this study, we developed a serological assay based on the Luminex technology. Nine recombinant proteins representing different vir...

journal_title：Journal of clinical microbiology

authors： Ayouba A,Touré A,Butel C,Keita AK,Binetruy F,Sow MS,Foulongne V,Delaporte E,Peeters M

更新日期：2016-12-28 00:00:00

abstract：:Streptococcus pneumoniae is a major cause of bacteremia, meningitis, pneumonia, sinusitis, and acute otitis media in children. Although optochin susceptibility, bile solubility, and Quellung testing are the standards for identifying and differentiating pneumococci, there are several reports of nontypeable pneumococci ...

journal_title：Journal of clinical microbiology

authors： Ing J,Mason EO,Kaplan SL,Lamberth LB,Revell PA,Luna RA,Hulten KG

更新日期：2012-04-01 00:00:00

abstract：:A multiresistant strain of Pseudomonas aeruginosa is widespread among cystic fibrosis (CF) patients attending clinics in Liverpool, United Kingdom. Suppression subtractive hybridization was used to identify sequences present in the Liverpool CF epidemic strain but absent from strain PAO1. Using dot blot and PCR amplif...

journal_title：Journal of clinical microbiology

authors： Parsons YN,Panagea S,Smart CH,Walshaw MJ,Hart CA,Winstanley C

更新日期：2002-12-01 00:00:00

abstract：:Infective endocarditis (IE) is a life-threatening infection of the heart endothelium and valves. Staphylococcus aureus is a predominant cause of severe IE and is frequently associated with infections in health care settings and device-related infections. Multilocus sequence typing (MLST), spa typing, and virulence gen...

journal_title：Journal of clinical microbiology

authors： Nethercott C,Mabbett AN,Totsika M,Peters P,Ortiz JC,Nimmo GR,Coombs GW,Walker MJ,Schembri MA

更新日期：2013-07-01 00:00:00

abstract：:Campylobacter rectus was isolated under routine anaerobic conditions (no additional hydrogen gas in the atmosphere) from an oral, nonperiodontal abscess from a patient with gastroesophageal adenocarcinoma. We report the first case of a palate abscess caused by C. rectus and review the literature and atmospheric requir...

journal_title：Journal of clinical microbiology

authors： Mahlen SD,Clarridge JE 3rd

更新日期：2009-03-01 00:00:00

abstract：:Trauma intensive care unit (TICU) patients requiring mechanical respiratory support frequently develop ventilator-associated pneumonia (VAP). Oral and oropharyngeal bacteria are believed to be responsible for many cases of VAP, but definitive evidence of this relationship is lacking. Earlier studies used conventional ...

journal_title：Journal of clinical microbiology

authors： Bahrani-Mougeot FK,Paster BJ,Coleman S,Barbuto S,Brennan MT,Noll J,Kennedy T,Fox PC,Lockhart PB

更新日期：2007-05-01 00:00:00

abstract：:Automated treponemal immunoassays are used for syphilis screening with the reverse-sequence algorithm; discordant results (e.g., enzyme immunoassay [EIA] reactive and reactive plasma reagin [RPR] nonreactive) are resolved with a second treponemal test. We conducted a study to determine automated immunoassay signal str...

journal_title：Journal of clinical microbiology

authors： Fakile YF,Jost H,Hoover KW,Gustafson KJ,Novak-Weekley SM,Schapiro JM,Tran A,Chow JM,Park IU

更新日期：2017-12-26 00:00:00

abstract：:Yersinia enterocolitica serovar O:3 bacteria with and without plasmid-encoded proteins were compared as antigens in an enzyme-linked immunosorbent assay. Good correlations between the two antigen preparations were obtained for immunoglobulin M (IgM), IgG, and IgA antibodies of patients with yersiniosis. For routine di...

journal_title：Journal of clinical microbiology

authors： Granfors K,Lahesmaa-Rantala R,Ståhlberg TH,Toivanen A

更新日期：1989-03-01 00:00:00

abstract：:The use of different assay conditions has complicated the evaluation of studies relating salivary lysozyme levels to oral or systemic disease. The purpose of this study was to compare values obtained for lysozyme activity in mixed saliva of 104 healthy subjects by using two assay techniques and four variations in samp...

journal_title：Journal of clinical microbiology

authors： Jenzano JW,Hogan SL,Lundblad RL

更新日期：1986-12-01 00:00:00

abstract：:The Scottish Meningococcus and Pneumococcus Reference Laboratory (SMPRL) provides a national service for the laboratory confirmation of meningococcal and pneumococcal disease in Scotland. Part of this service includes the serogrouping of meningococcal isolates followed by typing and subtyping. The procedures for this ...

journal_title：Journal of clinical microbiology

authors： Clarke SC,Diggle MA,Edwards GF

更新日期：2001-09-01 00:00:00

abstract：:A new approach, short-oligonucleotide-ligation assay on DNA chip (SOLAC), is developed to detect mutations in rifampin-resistant Mycobacterium tuberculosis. The method needs only four common probes to detect 15 mutational variants of the rpoB gene within 12 h. Fifty-five rifampin-resistant M. tuberculosis isolates wer...

journal_title：Journal of clinical microbiology

authors： Deng JY,Zhang XE,Lu HB,Liu Q,Zhang ZP,Zhou YF,Xie WH,Fu ZJ

更新日期：2004-10-01 00:00:00

abstract：:Vulvovaginal candidiasis (VVC) remains a common cause of morbidity, with three-quarters of women affected during their lifetimes. Use of antibiotics is an acknowledged trigger for VVC, which adversely affects women's physical and emotional health. Knowledge of patterns of genital Candida species-level identification i...

journal_title：Journal of clinical microbiology

authors： Pirotta MV,Garland SM

更新日期：2006-09-01 00:00:00

abstract：:We have used comparative genomic hybridization (CGH) on a full-genome Campylobacter jejuni microarray to examine genome-wide gene conservation patterns among 51 strains isolated from food and clinical sources. These data have been integrated with data from three previous C. jejuni CGH studies to perform a meta-analysi...

journal_title：Journal of clinical microbiology

authors： Taboada EN,Acedillo RR,Carrillo CD,Findlay WA,Medeiros DT,Mykytczuk OL,Roberts MJ,Valencia CA,Farber JM,Nash JH

更新日期：2004-10-01 00:00:00

abstract：:High incidence rates of isoniazid-, rifampin-, and multiple-drug-resistant tuberculosis have been reported in countries of the former Soviet Union (FSU). Genotypic (unlike phenotypic) drug resistance assays do not require viable cultures but require accurate knowledge of both the target gene and the mutations associat...

journal_title：Journal of clinical microbiology

authors： Nikolayevsky V,Brown T,Balabanova Y,Ruddy M,Fedorin I,Drobniewski F

更新日期：2004-10-01 00:00:00

abstract：:A fundamental role for the endosymbiotic bacteria Wolbachia pipientis in the pathogenesis of Dirofilaria immitis infections has emerged in recent years. Diagnostic opportunities arising from this breakthrough have not yet been fully exploited. This study was aimed at developing conventional and real-time PCR assays to...

journal_title：Journal of clinical microbiology

authors： Turba ME,Zambon E,Zannoni A,Russo S,Gentilini F

更新日期：2012-08-01 00:00:00