Molecular characterization of endocarditis-associated Staphylococcus aureus.

abstract：:Accreditation in Ontario, Canada, requires that licensed clinical laboratories participate in external quality assessment (also known as proficiency testing) and perform competency evaluation of their staff. To assess the extent of ongoing competency assessment practices, the Quality Management Program--Laboratory Ser...

journal_title：Journal of clinical microbiology

authors： Desjardins M,Fleming CA

更新日期：2014-08-01 00:00:00

abstract：:A single multiplex PCR assay targeting seven virulence factors and the wzi gene specific for the K1 and K2 capsular serotypes of Klebsiella pneumoniae was developed and tested on 65 clinical isolates, which included 45 isolates responsible for community-acquired severe human infections. The assay is useful for the sur...

journal_title：Journal of clinical microbiology

authors： Compain F,Babosan A,Brisse S,Genel N,Audo J,Ailloud F,Kassis-Chikhani N,Arlet G,Decré D

更新日期：2014-12-01 00:00:00

abstract：:The human metapneumovirus (hMPV) was recently identified and linked to acute respiratory tract infections (ARTI). To assess the clinical importance of this virus in infants and children, we developed a rapid and efficient reverse transcription-PCR-based screening method for a large volume of samples and tested retrosp...

journal_title：Journal of clinical microbiology

authors： Chano F,Rousseau C,Laferrière C,Couillard M,Charest H

更新日期：2005-11-01 00:00:00

abstract：:Evaluations of screening tests for bacteriuria have traditionally compared the test results with those of quantitative urine cultures. However, many patients with symptomatic urinary tract infections can have less than 10(5) CFU/ml in their urine. Therefore, the results of urine culture and three screening tests (Bac-...

journal_title：Journal of clinical microbiology

authors： Murray PR,Smith TB,McKinney TC Jr

更新日期：1987-03-01 00:00:00

abstract：:Trichomonas vaginalis is the most prevalent nonviral sexually transmitted infection worldwide, and improved diagnostic methods are critical for controlling this pathogen. Diagnostic assays that can be used in conjunction with routine chlamydia/gonorrhea nucleic acid-based screening are likely to have the most impact o...

journal_title：Journal of clinical microbiology

authors： Van Der Pol B,Williams JA,Taylor SN,Cammarata CL,Rivers CA,Body BA,Nye M,Fuller D,Schwebke JR,Barnes M,Gaydos CA

更新日期：2014-03-01 00:00:00

abstract：:A fully automated antifungal susceptibility test system recently updated to reflect the new species-specific clinical breakpoints (CBPs) of fluconazole for Candida (Vitek 2 AF03 yeast susceptibility test; bioMérieux, Inc., Durham, NC) was compared in three different laboratories with the Clinical and Laboratory Standa...

journal_title：Journal of clinical microbiology

authors： Pfaller MA,Diekema DJ,Procop GW,Wiederhold NP

更新日期：2014-06-01 00:00:00

abstract：:The use of monoclonal antibody (MAb) p63-27, which is reactive with the major immediate-early human cytomegalovirus (HCMV) protein pp72, was explored for the rapid diagnosis of HCMV viruria. The rapid assay detected all but 1 of 19 specimens identified by standard virus isolation methods from 1,676 newborn urine speci...

journal_title：Journal of clinical microbiology

authors： Boppana SB,Smith RJ,Stagno S,Britt WJ

更新日期：1992-03-01 00:00:00

abstract：:The results of single radial immunodiffusion assays of influenza virus hemagglutinin were found to be greatly altered by small antigenic differences between test and reference strains. When such differences were present, the precise specificity of the antiserum used had a critical effect on the measured hemagglutinin ...

journal_title：Journal of clinical microbiology

authors： Rodda SJ,Gallichio HA,Hampson AW

更新日期：1981-11-01 00:00:00

abstract：:Despite its theoretical advantages, direct antimicrobial susceptibility testing (DST) of urine specimens remains controversial largely because of concerns regarding its accuracy, particularly with mixed cultures. To evaluate the performance of DST in the setting of acute urinary tract infection (UTI), we performed DST...

journal_title：Journal of clinical microbiology

authors： Johnson JR,Tiu FS,Stamm WE

更新日期：1995-09-01 00:00:00

abstract：:We have developed an easy and rapid detection and identification system for the diagnosis of mycobacterial diseases. The system is based on selective amplification by PCR of mycobacteria with primers based on the genes coding for 16S rRNA. During PCR, a label (digoxigenin-11-dUTP) is incorporated with biotinylated spe...

journal_title：Journal of clinical microbiology

authors： De Beenhouwer H,Liang Z,De Rijk P,Van Eekeren C,Portaels F

更新日期：1995-11-01 00:00:00

abstract：:We evaluated the performance of three commercially available monoclonal antibodies for confirmation of the presence of Chlamydia pneumoniae in cell culture by examining their abilities to stain inclusions of eight strains of C. pneumoniae. The antibodies tested were two unconjugated C. pneumoniae-specific monoclonal r...

journal_title：Journal of clinical microbiology

authors： Montalban GS,Roblin PM,Hammerschlag MR

更新日期：1994-05-01 00:00:00

abstract：:Porcine reproductive and respiratory syndrome has been devastating the swine industry since the late 1980s. The disease has been controlled, to some extent, through the use of modified live-attenuated (MLV) vaccines once available. However, such a practice periodically resulted in isolation or detection of vaccine-lik...

journal_title：Journal of clinical microbiology

authors： Key KF,Guenette DK,Yoon KJ,Halbur PG,Toth TE,Meng XJ

更新日期：2003-06-01 00:00:00

abstract：:Ten cases of typhoid fever occurred between 24 August and 1 September 1986 in the vicinity of Silver Spring, Md. Shrimp salad served in a fast-food restaurant was implicated as the source of infection. Stool cultures were obtained from 104 employees, and serum Vi antibodies were assayed in 97 of the employees. Salmone...

journal_title：Journal of clinical microbiology

authors： Lin FY,Becke JM,Groves C,Lim BP,Israel E,Becker EF,Helfrich RM,Swetter DS,Cramton T,Robbins JB

更新日期：1988-06-01 00:00:00

abstract：:We compared the microhemagglutination assay for Treponema pallidum (MHA-TP), a treponemal test, with two other treponemal tests, the Serodia Treponema pallidum particle agglutination (TP-PA) assay and the Captia Syphilis-G enzyme immunoassay, using 390 clinical serum samples. We also compared two nontreponemal tests, ...

journal_title：Journal of clinical microbiology

authors： Pope V,Fears MB,Morrill WE,Castro A,Kikkert SE

更新日期：2000-07-01 00:00:00

abstract：:We have developed a method for determining the serotypes of poliovirus isolates by PCR. Three sets of serotype-specific antisense PCR-initiating primers (primers seroPV1A, seroPV2A, and seroPV3A) were designed to pair with codons of VP1 amino acid sequences that are conserved within but that differ across serotypes. T...

journal_title：Journal of clinical microbiology

authors： Kilpatrick DR,Nottay B,Yang CF,Yang SJ,Da Silva E,Peñaranda S,Pallansch M,Kew O

更新日期：1998-02-01 00:00:00

abstract：:We developed a liquid bead microarray (LBMA) assay for genotyping genital human papillomaviruses (HPVs) based on the MY09-MY11-HMB01 PCR system and the reverse line blot (RLB) assay probe sequences. Using individual HPV plasmids, we were able to detect as few as 50 copies per reaction. In two separate retrospective st...

journal_title：Journal of clinical microbiology

authors： Feng Q,Cherne S,Winer RL,Balasubramanian A,Lee SK,Hawes SE,Kiviat NB,Koutsky LA

更新日期：2009-03-01 00:00:00

abstract：:The sensitivities of BHK-21 (C-13) and murine neuroblastoma (C-1300; clone NA) cells for the isolation of small quantities of a street strain rabies virus were compared. Suspensions of brain from mice sacrificed prior to the onset of clinical signs of rabies were used to stimulate weakly positive diagnostic specimens....

journal_title：Journal of clinical microbiology

authors： Rudd RJ,Trimarchi CV

更新日期：1987-08-01 00:00:00

abstract：:Vaginal discharge specimens from 105 women were examined by wet mount, culture, and a new direct immunofluorescence assay to detect Trichomonas vaginalis. The organism was detected by culture in 31 patients, by direct immunofluorescence in 26 patients, and by wet mount in 21 patients. ...

journal_title：Journal of clinical microbiology

authors： Smith RF

更新日期：1986-12-01 00:00:00

abstract：:Four serotypes of Cryptococcus neoformans designated A, B, C, and D are currently recognized. Although an agglutination test is most often used to serotype C. neoformans in cultures, this test is not appropriate for typing the fungus in fixed tissues. A study to prepare fluorescent-antibody reagents for typing C. neof...

journal_title：Journal of clinical microbiology

authors： Kaplan W,Bragg SL,Crane S,Ahearn DG

更新日期：1981-09-01 00:00:00

abstract：:With improved measles virus (MV) control, the genetic variability of the MV-nucleoprotein hypervariable region (NP-HVR) decreases. Thus, it becomes increasingly difficult to determine the origin of a virus using only this part of the genome. During outbreaks in Europe and Africa, we found MV strains with identical NP-...

journal_title：Journal of clinical microbiology

authors： Kessler JR,Kremer JR,Shulga SV,Tikhonova NT,Santibanez S,Mankertz A,Semeiko GV,Samoilovich EO,Tamfum JJ,Pukuta E,Muller CP

更新日期：2011-02-01 00:00:00

abstract：:A rapid and accurate method to identify the species and antibiotic resistance of Candida spp. in blood is vital to increase the survival rates of patients with bloodstream infections. However, the extremely low levels of Candida spp. in blood make rapid diagnosis by standard blood culture difficult. In this study, we ...

journal_title：Journal of clinical microbiology

authors： Chen XL,Zheng H,Li WG,Zhong YH,Chen XP,Lu JX

更新日期：2020-03-25 00:00:00

abstract：:The genes encoding the 56-kDa polypeptides were amplified by polymerase chain reaction from the genomic DNAs of three serotypes of Rickettsia tsutsugamushi, Gilliam, Karp, and Boryong. The amplified products were cloned into expression vector pIH821, and the recombinant antigens were expressed in Escherichia coli as f...

journal_title：Journal of clinical microbiology

authors： Kim IS,Seong SY,Woo SG,Choi MS,Kang JS,Chang WH

更新日期：1993-08-01 00:00:00

abstract：:Spiroplasma species are organisms that normally colonize plants and insects. We describe the first case of human systemic infection caused by Spiroplasma bacteria in a patient with hypogammaglobulinemia undergoing treatment with biological disease-modifying antirheumatic agents. Spiroplasma turonicum was identified th...

journal_title：Journal of clinical microbiology

authors： Aquilino A,Masiá M,López P,Galiana AJ,Tovar J,Andrés M,Gutiérrez F

更新日期：2015-02-01 00:00:00

abstract：:Rapid tests for glucose, maltose, lactose, sucrose, and starch fermentation were performed on 112 strains of anaerobic bacteria. The tests were incubated under aerobic conditions, and results were read within 4 h. An overall correlation of 89% was achieved between the rapid tests and the Virginia Polytechnic Institut...

journal_title：Journal of clinical microbiology

authors： Schreckenberger PC,Blazevic DJ

更新日期：1976-03-01 00:00:00

abstract：:We studied the evolution of Vibrio cholerae O1 during the 1991 to 2000 cholera epidemic in Mexico by biochemical, serological, and molecular characterization of strains collected during this period. Strains were divided into toxigenic and nontoxigenic groups according to the presence or absence of genes encoding chole...

journal_title：Journal of clinical microbiology

authors： Lizárraga-Partida ML,Quilici ML

更新日期：2009-05-01 00:00:00

abstract：:A monoclonal antibody specific for group D Salmonella antigen 9 was used in an indirect enzyme-linked immunosorbent assay (ELISA) for detecting the antigen in urine specimens collected from patients with clinical typhoid fever in Jakarta, Indonesia. The ELISA had a sensitivity of 95% in identifying patients in whom Sa...

journal_title：Journal of clinical microbiology

authors： Chaicumpa W,Ruangkunaporn Y,Burr D,Chongsa-Nguan M,Echeverria P

更新日期：1992-09-01 00:00:00

abstract：:Given the rise in the incidence of invasive fungal infections (IFIs) and the expanding spectrum of fungal pathogens, early and accurate identification of the causative pathogen is essential. We developed a panfungal PCR assay that targets the internal transcribed spacer 1 (ITS1) region of the ribosomal DNA gene cluste...

journal_title：Journal of clinical microbiology

authors： Lau A,Chen S,Sorrell T,Carter D,Malik R,Martin P,Halliday C

更新日期：2007-02-01 00:00:00

abstract：:This report describes the first well-documented reported case of the recovery of Blastomyces dermatitidis from the blood of a patient with disseminated blastomycosis. The isolate was recovered by using a lysis-centrifugation method and inhibitory mold agar after 6 days of incubation at 30 degrees C. One of six blood s...

journal_title：Journal of clinical microbiology

authors： Musial CE,Wilson WR,Sinkeldam IR,Roberts GD

更新日期：1987-08-01 00:00:00

abstract：:We evaluated a broad-based PCR assay coupled with high-resolution melt analysis for rapid bacterial identification in patients with bacterial sepsis. With a reference library of 60 clinically relevant bacterial species, 52 positive blood culture samples were tested. Our assay identified 46/52 samples at the species le...

journal_title：Journal of clinical microbiology

authors： Won H,Rothman R,Ramachandran P,Hsieh YH,Kecojevic A,Carroll KC,Aird D,Gaydos C,Yang S

更新日期：2010-09-01 00:00:00

abstract：:Ten characteristic strains of spirochetes (HT2, HT7, HT10, HT15, HT17, HT19, HT20, HT22, HT32, and HT59) isolated from Ixodes persulcatus adult ticks in Hokkaido, Japan, were selected and analyzed by polyacrylamide gel electrophoresis, with monoclonal and polyclonal antibodies, and by pulsed-field gel electrophoresis....

journal_title：Journal of clinical microbiology

authors： Fukunaga M,Sohnaka M,Takahashi Y,Nakao M,Miyamoto K

更新日期：1993-05-01 00:00:00