Recovery of Blastomyces dermatitidis from blood of a patient with disseminated blastomycosis.

abstract：:Based on findings in small RNA-sequencing (Seq) data analysis, we developed highly sensitive and specific real-time reverse transcription (RT)-PCR assays with locked nucleic acid probes targeting the abundantly expressed leader sequences of Middle East respiratory syndrome coronavirus (MERS-CoV) and other human corona...

journal_title：Journal of clinical microbiology

authors： Chan JF,Choi GK,Tsang AK,Tee KM,Lam HY,Yip CC,To KK,Cheng VC,Yeung ML,Lau SK,Woo PC,Chan KH,Tang BS,Yuen KY

更新日期：2015-08-01 00:00:00

abstract：:Phosphatase activity was found to be applicable as a criterion for the differentiation of Mycoplasma salivarium and Mycoplasma orale, predominant constituents of oral mycoplasmal flora. Therefore, a simple procedure for the phosphatase activity assay was established as a screening test for the differentiation of oral ...

journal_title：Journal of clinical microbiology

authors： Shibata K,Totsuka M,Watanabe T

更新日期：1986-05-01 00:00:00

abstract：:A simple solid-phase enzyme-linked immunosorbent assay procedure for the detection of human antibodies to Entamoeba histolytica was developed which showed a high degree of correlation with the agar gel diffusion, counterelectrophoresis, and indirect hemagglutination methods, as well as with clinical data. The enzyme-l...

journal_title：Journal of clinical microbiology

authors： Lin TM,Halbert SP,Chiu CT,Zarco R

更新日期：1981-04-01 00:00:00

abstract：:Procedures for differentiation between wild and vaccine-derived strains of poliovirus are required, particularly in countries where wild and vaccine-related strains coexist. For this differentiation, we tested the method of Inouye and Hondo (S. Inouye and R. Hondo, Arch. Virol. 129:311-316, 1993) for discrimination of...

journal_title：Journal of clinical microbiology

authors： Takeda N,Sakae K,Agboatwalla M,Isomura S,Hondo R,Inouye S

更新日期：1994-01-01 00:00:00

abstract：:D-Arabinitol (DA) is a useful diagnostic marker for candidiasis in patients with neutropenia and other high-risk groups, but its use in unselected patients with a broad range of underlying diseases and conditions has not been studied. We used an automated enzymatic fluorometric assay to measure serum DA/creatinine rat...

journal_title：Journal of clinical microbiology

authors： Yeo SF,Huie S,Sofair AN,Campbell S,Durante A,Wong B

更新日期：2006-11-01 00:00:00

abstract：:A PCR specific for spacer regions 33 and 34 of the direct repeat region of the Mycobacterium tuberculosis complex was developed to complement the biochemical differentiation of M. tuberculosis, Mycobacterium bovis, M. bovis BCG, and Mycobacterium africanum subtypes I and II. In addition, this approach was incorporated...

journal_title：Journal of clinical microbiology

authors： Yeboah-Manu D,Yates MD,Wilson SM

更新日期：2001-11-01 00:00:00

abstract：:Twenty-nine Mycobacterium reference strains representing 10 species and 60 mycobacterial cultures isolated from sputum specimens were studied. These cultures were grown in Bactec 7H12B medium (Becton Dickinson and Co., Paramus, N.J.) supplemented with oleic acid-albumin-dextrose-catalase enrichment broth (Becton Dicki...

journal_title：Journal of clinical microbiology

authors： Chou S,Chedore P,Haddad A,Paul NR,Kasatiya S

更新日期：1996-05-01 00:00:00

abstract：:Sera from patients with verified systemic staphylococcal infection contained antibodies reactive with peptidoglycan (PG) from Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Micrococcus lysodeikticus, Bacillus subtilis, and Escherichia coli. The presence of anti-PG cross-reactive antibodies ...

journal_title：Journal of clinical microbiology

authors： Wergeland HI,Endresen C

更新日期：1987-03-01 00:00:00

abstract：:Determination of the G and P serotypes of group A bovine rotaviruses from 149 samples of feces or intestinal contents collected from calves showing clinical signs of neonatal diarrhea was performed by a nested reverse transcription-PCR typing assay. The G6 serotype was the most prevalent, accounting for viruses in 55....

journal_title：Journal of clinical microbiology

authors： Falcone E,Tarantino M,Di Trani L,Cordioli P,Lavazza A,Tollis M

更新日期：1999-12-01 00:00:00

abstract：:A DNA macroarray containing 465 intragenic amplicons was designed to identify Staphylococcus aureus at the species level and to type S. aureus isolates. The genes selected included those encoding (i) S. aureus-specific proteins, (ii) staphylococcal and enterococcal proteins mediating antibiotic resistance and factors ...

journal_title：Journal of clinical microbiology

authors： Trad S,Allignet J,Frangeul L,Davi M,Vergassola M,Couve E,Morvan A,Kechrid A,Buchrieser C,Glaser P,El-Solh N

更新日期：2004-05-01 00:00:00

abstract：:We compared 14 molecular assays for their ability to detect the Mycobacterium tuberculosis complex in bronchoalveolar lavage fluid samples. Three approaches were followed. First, by using DNA from Mycobacterium bovis BCG, we determined the detection limits of the assays using routine molecular methods. Second, in orde...

journal_title：Journal of clinical microbiology

authors： Akkerman OW,van der Werf TS,de Boer M,de Beer JL,Rahim Z,Rossen JW,van Soolingen D,Kerstjens HA,van der Zanden AG

更新日期：2013-11-01 00:00:00

abstract：:Quantitative microdilution plate hybridization was used to identify 22 Mycobacterium species. DNAs of clinical strains were rapidly extracted and labeled with photoreactive biotin. Labeled DNAs were distributed into wells of a microdilution plate in which reference DNAs had been immobilized. After 2 h of hybridization...

journal_title：Journal of clinical microbiology

authors： Kusunoki S,Ezaki T,Tamesada M,Hatanaka Y,Asano K,Hashimoto Y,Yabuuchi E

更新日期：1991-08-01 00:00:00

abstract：:A three-step method for the purification of Enterocytozoon bieneusi spores from stool specimens was developed. The primary process of purification of the spores from bacterial contaminants involved Percoll gradient centrifugation followed by additional separation using cesium chloride density gradient centrifugation. ...

journal_title：Journal of clinical microbiology

authors： Kucerova Z,Moura H,Leitch GJ,Sriram R,Bern C,Kawai V,Vargas D,Gilman RH,Ticona E,Vivar A,Visvesvara GS

更新日期：2004-07-01 00:00:00

abstract：:Swine influenza viruses (SIV) have been shown to sporadically infect humans and are infrequently identified by the Influenza Division of the Centers for Disease Control and Prevention (CDC) after being received as unsubtypeable influenza A virus samples. Real-time reverse transcriptase PCR (rRT-PCR) procedures for det...

journal_title：Journal of clinical microbiology

authors： Shu B,Wu KH,Emery S,Villanueva J,Johnson R,Guthrie E,Berman L,Warnes C,Barnes N,Klimov A,Lindstrom S

更新日期：2011-07-01 00:00:00

abstract：:Recent evidence supports a causal relationship between Bartonella (Rochalimaea) henselae, cat-scratch disease (CSD), and bacillary angiomatosis. Cats appear to be the primary reservoir. Blood from 19 cats owned by 14 patients diagnosed with CSD was cultured. Blood samples from cats owned by veterinary students (n = 25...

journal_title：Journal of clinical microbiology

authors： Kordick DL,Wilson KH,Sexton DJ,Hadfield TL,Berkhoff HA,Breitschwerdt EB

更新日期：1995-12-01 00:00:00

abstract：:The Digene Hybrid Capture System (DHCS) is a solution hybridization antibody capture assay for the chemiluminescent detection and quantitation of cytomegalovirus (CMV) DNA in leukocytes. This assay was compared with the CMV antigenemia assay and shell vial and tube cultures for the detection of CMV in 234 blood specim...

journal_title：Journal of clinical microbiology

authors： Mazzulli T,Wood S,Chua R,Walmsley S

更新日期：1996-12-01 00:00:00

abstract：:Disseminated disease by Aspergillus granulosus has been reported only once previously in a cardiac transplant recipient. We report a fatal central nervous system infection in a lung transplant recipient. Key features of this species in the section Usti include growth at 37 degrees C and large, randomly spaced aggregat...

journal_title：Journal of clinical microbiology

authors： Sutton DA,Wickes BL,Romanelli AM,Rinaldi MG,Thompson EH,Fothergill AW,Dishop MK,Elidemir O,Mallory GB,Moonnamakal SP,Adesina AM,Schecter MG

更新日期：2009-10-01 00:00:00

abstract：:Ureaplasma urealyticum has been associated with urethritis in men, obstetric problems in women, and respiratory distress syndrome in preterm infants. U. urealyticum can be divided into two biovars comprising 14 serovars. Partial sequences of genes encoding the multiple-banded antigens of the cell surface are known. Us...

journal_title：Journal of clinical microbiology

authors： Pitcher D,Sillis M,Robertson JA

更新日期：2001-05-01 00:00:00

abstract：:The reliability of the novel BACTEC MGIT 960 pyrazinamide (PZA) kit (Becton Dickinson Microbiology Systems, Sparks, Md.) was assessed for testing of susceptibility of Mycobacterium tuberculosis to PZA. Results generated by the BACTEC MGIT 960 system (Becton Dickinson) were compared with those obtained with the BACTEC ...

journal_title：Journal of clinical microbiology

authors： Pfyffer GE,Palicova F,Rüsch-Gerdes S

更新日期：2002-05-01 00:00:00

abstract：:Routine bacterial cultures of corneal scrapings from seven cats with either ulcerative feline keratitis, keratomalacia, or both yielded colonies which were identified by 16S rRNA gene sequencing as Mycoplasma felis (six cases) and Mycoplasma gateae (one case). Identification of the pathogens allowed the use of less em...

journal_title：Journal of clinical microbiology

authors： Gray LD,Ketring KL,Tang YW

更新日期：2005-07-01 00:00:00

abstract：:Serum antibody responses to influenza A virus infection were examined in 388 normal subjects during a trial of chemoprophylaxis in an outbreak of influenza A in 1980-1981 in which both A/H1N1 and A/H3N2 viruses circulated. Paired serum specimens obtained over a 6-week period were tested for antibodies to both A/H1N1 a...

journal_title：Journal of clinical microbiology

authors： Madore HP,Reichman RC,Dolin R

更新日期：1983-12-01 00:00:00

abstract：:To improve the understanding of the relative importance of serotypes of rotavirus in dehydrating diarrhea, we examined the correlation of clinical characteristics and disease severity with serotype in 2,441 diarrheal episodes among children younger than 2 years of age in rural Bangladesh. Of 764 rotavirus-associated e...

journal_title：Journal of clinical microbiology

authors： Bern C,Unicomb L,Gentsch JR,Banul N,Yunus M,Sack RB,Glass RI

更新日期：1992-12-01 00:00:00

abstract：:A novel PCR detection assay that amplifies the Helicobacter pylori-specific vacuolating cytotoxin gene (vacA) and thus enables rapid diagnosis of infection is described. Additionally, a real-time probe hybridization melting point analysis assay to detect all three mutations in the 23S rRNA gene associated with clarith...

journal_title：Journal of clinical microbiology

authors： Chisholm SA,Owen RJ,Teare EL,Saverymuttu S

更新日期：2001-04-01 00:00:00

abstract：:In the present study, a novel broad-range real-time PCR was developed for the rapid detection of human pathogenic fungi. The assay targets a part of the 28S large-subunit ribosomal RNA (rDNA) gene. We investigated its application for the most important human pathogenic fungal genera, including Aspergillus, Candida, Cr...

journal_title：Journal of clinical microbiology

authors： Vollmer T,Störmer M,Kleesiek K,Dreier J

更新日期：2008-06-01 00:00:00

abstract：:Yarrowia lipolytica is a weakly pathogenic yeast that is rarely isolated from the blood. We observed transient recurrent catheter-related fungemia attributable to this organism in a leukemic patient. The fungemia and accompanying fever subsided spontaneously. The data suggest that it might be possible to withhold spec...

journal_title：Journal of clinical microbiology

authors： Chang CL,Park TH,Lee EY,Lim YT,Son HC

更新日期：2001-03-01 00:00:00

abstract：:The use of IS6110 as a marker for molecular epidemiological studies is limited when a Mycobacterium tuberculosis isolate has five or fewer copies of IS6110. Restriction fragment length polymorphism analysis with a highly polymorphic GC-rich repetitive sequence located in the plasmid pTBN12 (PGRS RFLP) and spoligotypin...

journal_title：Journal of clinical microbiology

authors： Flores L,Jarlsberg LG,Kim EY,Osmond D,Grinsdale J,Kawamura M,Desmond E,Hopewell PC,Kato-Maeda M

更新日期：2010-02-01 00:00:00

abstract：:A screening assay for phospholipase C using a chromogenic substrate incorporated into agar medium is described. The assay directly visualizes phospholipase C activity of mycoplasma lysates and membranes on agar plates, or the activity may be measured by spectrophotometry. The results from the assay confirm the presenc...

journal_title：Journal of clinical microbiology

authors： De Silva NS,Quinn PA

更新日期：1987-04-01 00:00:00

abstract：:We used a nested PCR with Borrelia flagellin gene (flaB) primers and DNA sequencing to determine if Borrelia lonestari was present in Amblyomma americanum ticks removed from military personnel and sent to the Tick-Borne Disease Laboratory of the U.S. Army Center for Health Promotion and Preventive Medicine. In our pre...

journal_title：Journal of clinical microbiology

authors： Stromdahl EY,Williamson PC,Kollars TM Jr,Evans SR,Barry RK,Vince MA,Dobbs NA

更新日期：2003-12-01 00:00:00

abstract：:Knowledge of polyomavirus BK (BKV) genomic diversity has greatly expanded. The implications of BKV DNA sequence variation for the performance of molecular diagnostic assays is not well studied. We analyzed 184 publically available VP-1 sequences encompassing the BKV genomic region targeted by an in-house quantitative ...

journal_title：Journal of clinical microbiology

authors： Randhawa P,Kant J,Shapiro R,Tan H,Basu A,Luo C

更新日期：2011-12-01 00:00:00

abstract：:Cell line 293, a continuous line of transformed human embryonic kidney cells, has been recognized for its sensitivity in the isolation of adenoviruses, particularly the fastidious species 40 and 41, from stool specimens. To explore the possibility of using this cell line for the isolation of other viruses from clinica...

journal_title：Journal of clinical microbiology

authors： Brown M,Petric M

更新日期：1986-04-01 00:00:00