Development and Evaluation of Novel Real-Time Reverse Transcription-PCR Assays with Locked Nucleic Acid Probes Targeting Leader Sequences of Human-Pathogenic Coronaviruses.

Abstract:

:Based on findings in small RNA-sequencing (Seq) data analysis, we developed highly sensitive and specific real-time reverse transcription (RT)-PCR assays with locked nucleic acid probes targeting the abundantly expressed leader sequences of Middle East respiratory syndrome coronavirus (MERS-CoV) and other human coronaviruses. Analytical and clinical evaluations showed their noninferiority to a commercial multiplex PCR test for the detection of these coronaviruses.

journal_name

J Clin Microbiol

authors

Chan JF,Choi GK,Tsang AK,Tee KM,Lam HY,Yip CC,To KK,Cheng VC,Yeung ML,Lau SK,Woo PC,Chan KH,Tang BS,Yuen KY

doi

10.1128/JCM.01224-15

subject

Has Abstract

pub_date

2015-08-01 00:00:00

pages

2722-6

issue

8

eissn

0095-1137

issn

1098-660X

pii

JCM.01224-15

journal_volume

53

pub_type

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