Direct identification of Mycobacterium species in Bactec 7H12B medium by gas-liquid chromatography.

abstract：:The sensitivities and specificities of three immunoassays for the detection of herpes simplex virus type 2 (HSV-2)-specific immunoglobulin G antibodies in serum, including the one-strip rapid immunoblot assay (RIBA; Chiron Corporation) and two indirect enzyme immunosorbent assays (EIA; Gull Laboratories and Centocor),...

journal_title：Journal of clinical microbiology

authors： Groen J,Van Dijk G,Niesters HG,Van Der Meijden WI,Osterhaus AD

更新日期：1998-03-01 00:00:00

abstract：:Herpes simplex virus type 1 (HSV-1) encodes 11 envelope glycoproteins, of which glycoprotein G-1 (gG-1) induces a type-specific antibody response. Variability of the gG-1 gene among wild-type strains may be a factor of importance for a reliable serodiagnosis and typing of HSV-1 isolates. Here, we used a gG-1 type-spec...

journal_title：Journal of clinical microbiology

authors： Rekabdar E,Tunbäck P,Liljeqvist JA,Lindh M,Bergström T

更新日期：2002-09-01 00:00:00

abstract：:Staphylococcus epidermidis, a human commensal, is a common cause of bacteremia in immunocompromised patients with indwelling medical devices. We report a case of isolated cervical adenitis caused by S. epidermidis in an immunocompetent patient and comment on the presumed pathogenesis. ...

journal_title：Journal of clinical microbiology

authors： Ryan-Poirier K,Patrick CC

更新日期：1993-02-01 00:00:00

abstract：:A total of 1,335 Escherichia coli strains isolated from patients with diarrhea were submitted to the Serény test. Of these strains, 1,035 were also submitted to slide agglutination test, using antisera for invasive E. coli serogroups. Of 325 isolates that were lysine decarboxylase negative, 138 reacted with one of the...

journal_title：Journal of clinical microbiology

authors： Toledo MR,Trabulsi LR

更新日期：1983-03-01 00:00:00

abstract：:A lymph node excision was performed on a 45-year-old woman with left cervical swelling. The disorder which developed after the patient had undergone oral surgery for a severe periodontal disease failed to respond to antimicrobial chemotherapy. A mycobacterial strain subsequently identified by high-performance liquid c...

journal_title：Journal of clinical microbiology

authors： Piersimoni C,Goteri G,Nista D,Mariottini A,Mazzarelli G,Bornigia S

更新日期：2004-08-01 00:00:00

abstract：:A selective medium was developed for the isolation of Bacteroides fragilis directly from ovine and bovine fecal samples. The medium (tryptose blood agar plus polymyxin B, triclosan (Irgasan), novobiocin, and nalidixic acid) permitted growth of B. fragilis and several other species of Bacteroides but inhibited growth o...

journal_title：Journal of clinical microbiology

authors： Border M,Firehammer BD,Shoop DS,Myers LL

更新日期：1985-03-01 00:00:00

abstract：:Yersinia enterocolitica biotype 1, serotype O:21 was isolated from feces or rectal washings of three members of one family in northwestern Saskatchewan. The three isolates gave positive pathogenicity tests in guinea pigs with cultures grown at 22 degrees C as inoculum. All three cases showed clinical symptoms consiste...

journal_title：Journal of clinical microbiology

authors： Martin T,Kasian GF,Stead S

更新日期：1982-10-01 00:00:00

abstract：:Meningococcal disease is normally suspected on clinical grounds and is confirmed by isolation of Neisseria meningitidis bacteria from blood or cerebrospinal fluid or, more recently, by serology or PCR of cerebrospinal fluid. Achieving confirmation of a clinical diagnosis of meningococcal disease has become more diffic...

journal_title：Journal of clinical microbiology

authors： Newcombe J,Cartwright K,Palmer WH,McFadden J

更新日期：1996-07-01 00:00:00

abstract：:The production and characterization of five monoclonal antibodies to Mycobacterium tuberculosis are described. Specificity of the monoclonal antibodies was tested against other mycobacterial species by an enzyme-linked immunosorbent assay and immunoblots. HGT 3a, an immunoglobulin M (IgM) antibody, recognizes a molecu...

journal_title：Journal of clinical microbiology

authors： Kadival GV,Chaparas SD

更新日期：1987-01-01 00:00:00

abstract：:We have developed and evaluated a new method to quantify human immunodeficiency virus type 2 (HIV-2) proviral DNA based on LightCycler real-time PCR. The assay has a detection limit of 5 copies/10(5) peripheral blood mononuclear cells (PBMC) and is insensitive to HIV-2 strain variability: HIV-2 subtypes A and B are bo...

journal_title：Journal of clinical microbiology

authors： Damond F,Descamps D,Farfara I,Telles JN,Puyeo S,Campa P,Leprêtre A,Matheron S,Brun-Vezinet F,Simon F

更新日期：2001-12-01 00:00:00

abstract：:A duplex real-time SYBR Green LightCycler PCR (LC-PCR) assay with DNA extraction using the QIAamp DNA Stool Mini kit was evaluated with regard to detection of 8 of 17 species of food- or waterborne pathogens in five stool specimens in 2 h or less. The protocol used the same LC-PCR with 20 pairs of specific primers. Th...

journal_title：Journal of clinical microbiology

authors： Fukushima H,Tsunomori Y,Seki R

更新日期：2003-11-01 00:00:00

abstract：:The penicillin-induced L phase of growth of the group B, type III streptococcal strain 76-043 was examined for biochemical properties used for the identification of group B streptococci. After numerous serial subcultures in the cell wall-defective state, this stable L phase continued to produce hemolysin, hippuricase,...

journal_title：Journal of clinical microbiology

authors： Flores AE,Ferrieri P

更新日期：1983-10-01 00:00:00

abstract：:At various times after orthopedic operations (more than a few weeks, with an average of 29.9 days), 11 patients had a sudden onset of high temperature (average 38.9 degrees C) and local cellulitis at different sites on the operated sides. The wounds had completely healed, without complicated infections, when the cellu...

journal_title：Journal of clinical microbiology

authors： Kitamura T,Kawamura Y,Ohkusu K,Masaki T,Iwashita H,Sawa T,Fujii S,Okamoto T,Akaike T

更新日期：2007-01-01 00:00:00

abstract：:The taxonomic status and structure of Streptococcus dysgalactiae have been the object of much confusion. Bacteria belonging to this species are usually referred to as Lancefield group C or group G streptococci in clinical settings in spite of the fact that these terms lack precision and prevent recognition of the exac...

journal_title：Journal of clinical microbiology

authors： Jensen A,Kilian M

更新日期：2012-01-01 00:00:00

abstract：:E test strips (AB Biodisk, Culver City, Calif.) tested on 2% NaCl-supplemented Mueller-Hinton agar and Alamar panels (Alamar, Sacramento, Calif.) correctly characterized 127 of 127 methicillin-resistant Staphylococcus aureus isolates and 100 of 100 methicillin-susceptible S. aureus isolates. These overnight antimicrob...

journal_title：Journal of clinical microbiology

authors： Novak SM,Hindler J,Bruckner DA

更新日期：1993-11-01 00:00:00

abstract：:Antisera prepared against the eight recognized serotypes of Ureaplasma urealyticum were tested against the homologous and heterologous antigens by a modified metabolic inhibition test that used bromothymol blue broth in microtiter plates. The method gives clear endpoint determinations which are usually maintained on c...

journal_title：Journal of clinical microbiology

authors： Robertson JA,Stemke GW

更新日期：1979-06-01 00:00:00

abstract：:First-catch early morning urine samples from patients attending a genitourinary medicine clinic were tested by three different enzyme immunoassays. The results suggest significant differences in the sensitivities and specificities of the different assays. The direct visualization of elementary bodies in urine deposits...

journal_title：Journal of clinical microbiology

authors： Paul ID,Caul EO

更新日期：1990-02-01 00:00:00

abstract：:When testing 248 clinical isolates of Neisseria gonorrhoeae, the sensitivity was 100% with GonoGen (Becton Dickinson Microbiology Systems), 99.6% (247 of 248) with GonoGen II (Becton Dickinson), 97.2% (241 of 248) with the MicroTrak direct fluorescent-antibody test (Syva), and 97.6% (242 of 248) with Rapid Fermentatio...

journal_title：Journal of clinical microbiology

authors： Kellogg JA,Orwig LK

更新日期：1995-02-01 00:00:00

abstract：:The use of centrifugation (700 x g, 60 min) in a plaque assay markedly increased (mean, 2.9-fold) the infectivity of all 42 influenza virus strains tested, compared with no centrifugation. Of 13 influenza virus strains isolated from 390 clinical specimens, 9 (69%) were efficiently isolated by the centrifugation assay ...

journal_title：Journal of clinical microbiology

authors： Seno M,Kanamoto Y,Takao S,Takei N,Fukuda S,Umisa H

更新日期：1990-07-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay (ELISA) for the detection and quantitation of human immunoglobulin M (IgM), IgG, and IgA antibodies against Yersinia enterocolitica by using lipopolysaccharides as antigens is described. The results obtained with the lipopolysaccharide ELISA were compared with the results of the wh...

journal_title：Journal of clinical microbiology

authors： Granfors K,Viljanen MK,Toivanen A

更新日期：1981-07-01 00:00:00

abstract：:Borrelia lusitaniae is a species within the complex Borrelia burgdorferi sensu lato and is infrequently isolated in Europe. In contrast, this species is by far the most predominant in North Africa and in Portugal. In this study, we analyzed the genetic diversity, at several loci, of a large population of isolates from...

journal_title：Journal of clinical microbiology

authors： Younsi H,Sarih M,Jouda F,Godfroid E,Gern L,Bouattour A,Baranton G,Postic D

更新日期：2005-04-01 00:00:00

abstract：:The objective of this study was to evaluate the clinical characteristics and outcomes of hospitalized patients tested for Clostridium difficile and determine the correlation between pretest probability for C. difficile infection (CDI) and assay results. Patients with testing ordered for C. difficile were enrolled and ...

journal_title：Journal of clinical microbiology

authors： Kwon JH,Reske KA,Hink T,Burnham CA,Dubberke ER

更新日期：2017-02-01 00:00:00

abstract：:The extent of genetic and antigenic variation found in a population of human parainfluenza virus type 1 (HPIV-1) during a single local epidemic was investigated. Fifteen HPIV-1 strains isolated from children in 1991 were analyzed. Nucleotide sequence variation in the hemagglutinin-neuraminidase protein (HN) gene demon...

journal_title：Journal of clinical microbiology

authors： Henrickson KJ,Savatski LL

更新日期：1996-03-01 00:00:00

abstract：:Naturally acquired infection of humans with a marine mammal-associated Brucella sp. has only been reported once previously in a study describing infections of two patients from Peru. We report the isolation and characterization of a strain of Brucella from a New Zealand patient that appears most closely related to str...

journal_title：Journal of clinical microbiology

authors： McDonald WL,Jamaludin R,Mackereth G,Hansen M,Humphrey S,Short P,Taylor T,Swingler J,Dawson CE,Whatmore AM,Stubberfield E,Perrett LL,Simmons G

更新日期：2006-12-01 00:00:00

abstract：:Seventy-eight blood cultures with a Gram stain result of Gram-positive cocci in pairs and/or chains were evaluated with the Nanosphere Verigene Gram-positive blood culture (BC-GP) assay. The overall concordance of the assay with culture was 89.7% (70/78 cultures), allowing for the development of a targeted treatment a...

journal_title：Journal of clinical microbiology

authors： Alby K,Daniels LM,Weber DJ,Miller MB

更新日期：2013-11-01 00:00:00

abstract：:Genomes of TT virus (TTV) and TTV-like minivirus DNA were detected in 80% and 61% of cervical swabs from healthy women, respectively, regardless of concurrent human papillomavirus infection. These results show that the potential exists for sexual transmission of TTV and related viruses. ...

journal_title：Journal of clinical microbiology

authors： Fornai C,Maggi F,Vatteroni ML,Pistello M,Bendinelli M

更新日期：2001-05-01 00:00:00

abstract：:Accurate and timely diagnosis of dengue virus (DEN) infections is essential for the differential diagnosis of patients with febrile illness and hemorrhagic fever. In the present study, the diagnostic value of a newly developed immune-complex dissociated nonstructural-1 (NS-1) antigen dot blot immunoassay (DBI) was com...

journal_title：Journal of clinical microbiology

authors： Koraka P,Burghoorn-Maas CP,Falconar A,Setiati TE,Djamiatun K,Groen J,Osterhaus AD

更新日期：2003-09-01 00:00:00

abstract：:Reactivation of latent varicella-zoster virus (VZV), presenting as localized zoster or as disseminated infection, is a common and potentially serious complication in hematopoietic stem cell transplantation (HSCT) recipients. We retrospectively studied anti-VZV immunoglobulin G titers by the immune adherence hemaggluti...

journal_title：Journal of clinical microbiology

authors： Onozawa M,Hashino S,Takahata M,Fujisawa F,Kawamura T,Nakagawa M,Kahata K,Kondo T,Ota S,Tanaka J,Imamura M,Asaka M

更新日期：2006-12-01 00:00:00

abstract：:Zika virus (ZIKV) is a mosquito-borne flavivirus that is responsible for recent explosive epidemics in the Americas. Notably, ZIKV infection during pregnancy has been found to cause congenital birth defects, including microcephaly, and ZIKV has been associated with Guillain-Barré syndrome in adults. Diagnosis and surv...

journal_title：Journal of clinical microbiology

authors： Balmaseda A,Zambrana JV,Collado D,García N,Saborío S,Elizondo D,Mercado JC,Gonzalez K,Cerpas C,Nuñez A,Corti D,Waggoner JJ,Kuan G,Burger-Calderon R,Harris E

更新日期：2018-02-22 00:00:00

abstract：:Sputum obtained from patients with cystic fibrosis (CF) is highly viscous and often heterogeneous in bacterial distribution. Adding dithiothreitol (DTT) is the standard method for liquefaction prior to processing sputum for molecular detection assays. To determine if DTT treatment homogenizes the bacterial distributio...

journal_title：Journal of clinical microbiology

authors： Stokell JR,Khan A,Steck TR

更新日期：2014-07-01 00:00:00