Purification of Enterocytozoon bieneusi spores from stool specimens by gradient and cell sorting techniques.

abstract：:The aim of this study was to develop a PCR and reverse line blot hybridization (PCR-RLB) macroarray assay based on 16S-23S rRNA gene internal transcribed spacer sequences for the identification and differentiation of 34 mycobacterial species or subspecies. The performance of the PCR-RLB assay was assessed and validate...

journal_title：Journal of clinical microbiology

authors： Xiong L,Kong F,Yang Y,Cheng J,Gilbert GL

更新日期：2006-10-01 00:00:00

abstract：:A fully automated microparticle enzyme immunoassay (EIA), IMx Core, was developed for the detection of antibody against hepatitis B core antigen (anti-HBc). IMx Core sensitivity was less than 0.5 Paul Ehrlich Institut units per ml and was greater than that of the commercial radioimmunoassay (RIA) or EIA, Corab and Cor...

journal_title：Journal of clinical microbiology

authors： Spronk AM,Schmidt L,Krenc C,Pavlis-Jenkins L,Brady J,Taskar S,Angus-Finn L,Mimms L

更新日期：1991-03-01 00:00:00

abstract：:Naturally acquired murine cytomegalovirus (MCMV) infection in laboratory strains of mice induces antibody levels which are generally undetectable by standard techniques; therefore, MCMV has not been included routinely in mouse viral antibody screening programs. The relative sensitivity of three assay systems, the nucl...

journal_title：Journal of clinical microbiology

authors： Anderson CA,Murphy JC,Fox JG

更新日期：1983-10-01 00:00:00

abstract：:Pseudomonas mendocina has been isolated from soil and water samples. Although it has been recovered from some human clinical samples, its pathogenic role has not yet been documented. We report the first known case of endocarditis in humans due to P. mendocina. ...

journal_title：Journal of clinical microbiology

authors： Aragone MR,Maurizi DM,Clara LO,Navarro Estrada JL,Ascione A

更新日期：1992-06-01 00:00:00

abstract：:Detection of human papillomavirus (HPV) E6/E7 oncogene expression may be more predictive of cervical cancer risk than testing for HPV DNA. The Aptima HPV test (Gen-Probe) detects E6/E7 mRNA of 14 oncogenic types. Its clinical performance was compared with that of the Hybrid Capture 2 DNA test (HC2; Qiagen) in women re...

journal_title：Journal of clinical microbiology

authors： Ratnam S,Coutlee F,Fontaine D,Bentley J,Escott N,Ghatage P,Gadag V,Holloway G,Bartellas E,Kum N,Giede C,Lear A

更新日期：2011-02-01 00:00:00

abstract：:Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has proved to be a useful diagnostic method for identifying conventional bacteria. In the case of mycobacteria, a good protein extraction protocol is essential in order to obtain reliable identification results. To date, no suc...

journal_title：Journal of clinical microbiology

authors： Rodriguez-Temporal D,Perez-Risco D,Struzka EA,Mas M,Alcaide F

更新日期：2018-03-26 00:00:00

abstract：:Genetic polymorphisms in the malaria parasite Plasmodium falciparum mediate alterations in sensitivity to important antimalarial drugs. Surveillance for these polymorphisms is helpful in assessing the prevalence of drug resistance and designing strategies for malaria control. Multiple methods are available for the ass...

journal_title：Journal of clinical microbiology

authors： LeClair NP,Conrad MD,Baliraine FN,Nsanzabana C,Nsobya SL,Rosenthal PJ

更新日期：2013-08-01 00:00:00

abstract：:Three antigenic variants of the H3N2 subtype of wild-type influenza A virus (representing the years 1968, 1972, and 1974) were examined for their sensitivity to interferon and for their ability to induce local respiratory tract interferon in volunteers. In addition, the time of appearance of symptoms in infected volun...

journal_title：Journal of clinical microbiology

authors： Richman DD,Murphy BR,Baron S,Uhlendorf C

更新日期：1976-03-01 00:00:00

abstract：:The prevalence of class D extended-spectrum oxacillinases (ES-OXAs) in ceftazidime-resistant strains of Pseudomonas aeruginosa is often underestimated by double-disk synergy tests (DDST) using clavulanate. A DDST with a customized distance between a disk of ceftazidime or cefepime and inhibitors (clavulanate and imipe...

journal_title：Journal of clinical microbiology

authors： Hocquet D,Dehecq B,Bertrand X,Plésiat P

更新日期：2011-06-01 00:00:00

abstract：:An enzyme immunoassay (enzyme-linked immunosorbent assay [ELISA]) to detect hamster antibody to toxins A and B of Clostridium difficile was developed in which toxin preparations are used to coat the solid phase. The specificity of the assay was supported by blocking tests with the toxin preparations and proteins from ...

journal_title：Journal of clinical microbiology

authors： Viscidi RP,Yolken RH,Laughon BE,Bartlett JG

更新日期：1983-08-01 00:00:00

abstract：:Stool microscopy as performed in clinical parasitology laboratories is a complex procedure with subjective interpretation. Quality assurance (QA) programs often emphasize proficiency testing as an assessment tool. We describe a result reproducibility assessment tool, which can form part of a broader QA program, and wh...

journal_title：Journal of clinical microbiology

authors： Libman MD,Gyorkos TW,Kokoskin E,Maclean JD

更新日期：2008-07-01 00:00:00

abstract：:Parameters like zone reading, inoculum density, and plate streaking influence the precision and accuracy of disk diffusion antibiotic susceptibility testing (AST). While improved reading precision has been demonstrated using automated imaging systems, standardization of the inoculum and of plate streaking have not bee...

journal_title：Journal of clinical microbiology

authors： Hombach M,Maurer FP,Pfiffner T,Böttger EC,Furrer R

更新日期：2015-12-01 00:00:00

abstract：:The PLEX-ID system is a novel technology that couples PCR amplification and electrospray ionization-mass spectrometry to identify pathogens directly in clinical specimens. The analytical performance of the PLEX-ID Broad Fungal assay was compared with that of traditional culture identification by using 91 characterized...

journal_title：Journal of clinical microbiology

authors： Simner PJ,Uhl JR,Hall L,Weber MM,Walchak RC,Buckwalter S,Wengenack NL

更新日期：2013-06-01 00:00:00

abstract：:A reverse transcription-PCR (RT-PCR) assay based on automated fluorescent capillary electrophoresis and GeneScan software analysis was developed to detect six common respiratory viruses in clinical specimens from young children. Assays for human respiratory syncytial virus (HRSV); human parainfluenza viruses 1, 2, and...

journal_title：Journal of clinical microbiology

authors： Erdman DD,Weinberg GA,Edwards KM,Walker FJ,Anderson BC,Winter J,González M,Anderson LJ

更新日期：2003-09-01 00:00:00

abstract：:Classical microbiological diagnosis of human brucellosis is time-consuming, hazardous, and subject to variable interpretation. Intact-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was evaluated for the routine identification of Brucella spp. Analysis of mass peak patt...

journal_title：Journal of clinical microbiology

authors： Karger A,Melzer F,Timke M,Bettin B,Kostrzewa M,Nöckler K,Hohmann A,Tomaso H,Neubauer H,Al Dahouk S

更新日期：2013-09-01 00:00:00

abstract：:Aeromonas caviae was recovered as the sole potential enteric pathogen from the stools of 14 of 17 symptomatic children (10 younger than 1 year of age) while Aeromonas hydrophila, Aeromonas sobria, and Plesiomonas shigelloides were isolated once each. The infants from whom A. caviae was isolated all presented with a wa...

journal_title：Journal of clinical microbiology

authors： Namdari H,Bottone EJ

更新日期：1990-05-01 00:00:00

abstract：:Staphylococcal cassette chromosome mec (SCCmec) types and Panton-Valentine leukocidin (PVL) gene carriage were compared among suspected community-associated methicillin-resistant Staphylococcus aureus MRSA (CA-MRSA) and health care-associated MRSA (HA-MRSA) isolates. CA-MRSA isolates carried the SCCmec type IV complex...

journal_title：Journal of clinical microbiology

authors： Moroney SM,Heller LC,Arbuckle J,Talavera M,Widen RH

更新日期：2007-03-01 00:00:00

abstract：:A panel of new cytomegalovirus (CMV) assays for use on the Architect instrument has been developed, including a CMV avidity assay based on a new technology. The purpose of this study was to compare the performance characteristics of the fully automated CMV immunoglobulin M (IgM), IgG, and IgG avidity tests on the Arch...

journal_title：Journal of clinical microbiology

authors： Lagrou K,Bodeus M,Van Ranst M,Goubau P

更新日期：2009-06-01 00:00:00

abstract：:We examined mutations in the dihydropteroate synthase (DHPS) genes of Pneumocystis carinii f. sp. hominis (P. carinii) strains isolated from 24 patients with P. carinii pneumonia (PCP) in Japan. DHPS mutations were identified at amino acid positions 55 and/or 57 in isolates from 6 (25.0%) of 24 patients. The underlyin...

journal_title：Journal of clinical microbiology

authors： Takahashi T,Hosoya N,Endo T,Nakamura T,Sakashita H,Kimura K,Ohnishi K,Nakamura Y,Iwamoto A

更新日期：2000-09-01 00:00:00

abstract：:Anthopsis deltoidea was found to be the cause of an olecranon bursitis in a 79-year-old golfer. Serial histological sections of the olecranon bursa showed faintly stained, brown-walled, septate, hyphal elements in the centers of the necrotic debris. The combination of bursectomy and flucytosine treatment cured the inf...

journal_title：Journal of clinical microbiology

authors： Kwon-Chung KJ,Droller DD

更新日期：1984-08-01 00:00:00

abstract：:A study to compare the polymerase chain reaction (PCR) test with the cell culture method in diagnosing urogenital Chlamydia trachomatis infections was performed. From 497 patients (212 women, 285 men) attending an outpatient clinic for sexually transmitted diseases, a total of 814 samples (female patients, cervix and ...

journal_title：Journal of clinical microbiology

authors： Vogels WH,van Voorst Vader PC,Schröder FP

更新日期：1993-05-01 00:00:00

abstract：:Azole-resistant Aspergillus fumigatus is an increasing worldwide problem with major clinical implications. Surveillance is warranted to guide clinicians to provide optimal treatment to patients. To investigate azole resistance in clinical Aspergillus isolates in our institution, a Belgian university hospital, we condu...

journal_title：Journal of clinical microbiology

authors： Montesinos I,Argudín MA,Hites M,Ahajjam F,Dodémont M,Dagyaran C,Bakkali M,Etienne I,Jacobs F,Knoop C,Patteet S,Lagrou K

更新日期：2017-08-01 00:00:00

abstract：:Standard repetitive extragenic palindromic (REP)-PCR, enterobacterial repetitive intergenic consensus-PCR, and Salmonella enteritidis repetitive element-PCR methods for bacterial strain typing were performed with DNA extracted by boiling members of each of the currently recognized species of human viridans group strep...

journal_title：Journal of clinical microbiology

authors： Alam S,Brailsford SR,Whiley RA,Beighton D

更新日期：1999-09-01 00:00:00

abstract：:Superparamagnetic polymer particles precoated with sheep anti-mouse immunoglobulin G were coated with immunoglobulin G2 monoclonal antibodies to the K88 antigen of Escherichia coli (MAb-K88). These immunomagnetic particles (IMP) were used for isolation and identification of K88 antigen-positive (K88+) E. coli. The bac...

journal_title：Journal of clinical microbiology

authors： Lund A,Hellemann AL,Vartdal F

更新日期：1988-12-01 00:00:00

abstract：:We have developed a high-resolution melting (HRM) assay to scan for mutations in the rpoB, inhA, ahpC, and katG genes and/or promoter regions for the detection of rifampin and isoniazid resistance in Mycobacterium tuberculosis. For assay development, 23 drug-resistant isolates of M. tuberculosis having 29 different mu...

journal_title：Journal of clinical microbiology

authors： Ong DC,Yam WC,Siu GK,Lee AS

更新日期：2010-04-01 00:00:00

abstract：:There are several convenient and accurate molecular assays to detect respiratory bacterial infection. The NeoPlex RB-8 detection kit (NeoPlex RB-8) is a new multiplex real-time PCR assay that simultaneously detects Streptococcus pneumoniae, Mycoplasma pneumoniae, Chlamydophila pneumoniae, Legionella pneumophila, Haemo...

journal_title：Journal of clinical microbiology

authors： Kim JW,Hong SS,Lee IS,Chi HY,Kim SO,Kim HN,Hong SP

更新日期：2020-01-28 00:00:00

abstract：:Quantitative PCR (qPCR) is more sensitive than microscopy for detecting Pneumocystis jirovecii in bronchoalveolar lavage (BAL) fluid. We therefore developed a qPCR assay and compared the results with those of a routine immunofluorescence assay (IFA) and clinical data. The assay included automated DNA extraction, ampli...

journal_title：Journal of clinical microbiology

authors： Botterel F,Cabaret O,Foulet F,Cordonnier C,Costa JM,Bretagne S

更新日期：2012-02-01 00:00:00

abstract：:Many central vascular catheters (CVCs) are removed unnecessarily because current diagnostic methods for CVC-associated infection are unreliable. A quantitative PCR assay using primers and probe targeted to bacterial 16S ribosomal DNA was used to measure the levels of bacterial DNA in blood samples drawn through the CV...

journal_title：Journal of clinical microbiology

authors： Warwick S,Wilks M,Hennessy E,Powell-Tuck J,Small M,Sharp J,Millar MR

更新日期：2004-04-01 00:00:00

abstract：:We report a case of disseminated Scedosporium/Pseudallescheria infection due to Pseudallescheria boydii sensu stricto after lung transplantation in a patient with cystic fibrosis. Dissemination occurred under voriconazole. Despite surgery and combination therapy with voriconazole, caspofungin, and terbinafine, the pat...

journal_title：Journal of clinical microbiology

authors： Morio F,Horeau-Langlard D,Gay-Andrieu F,Talarmin JP,Haloun A,Treilhaud M,Despins P,Jossic F,Nourry L,Danner-Boucher I,Pattier S,Bouchara JP,Le Pape P,Miegeville M

更新日期：2010-05-01 00:00:00

abstract：:A new compound, indoxyl-beta-D-glucuronide, was assessed as a substrate for the rapid detection of Escherichia coli in urine. Incorporation of this compound into MacConkey agar allowed the direct differentiation of E. coli as deep blue colonies distinct from lactose and nonlactose fermenters. The sensitivity was 88 to...

journal_title：Journal of clinical microbiology

authors： Delisle GJ,Ley A

更新日期：1989-04-01 00:00:00