Evaluation of the new architect cytomegalovirus immunoglobulin M (IgM), IgG, and IgG avidity assays.

abstract：:A urease-based enzyme-linked immunosorbent assay (ELISA) has been developed for the detection of reagin antibodies in serum. Visuwell Reagin (ADI Diagnostics Inc., Rexdale, Ontario, Canada) is a non-treponemal screening test for the serodiagnosis of syphilis which has the benefits of large batch testing, automatabilit...

journal_title：Journal of clinical microbiology

authors： White TJ,Fuller SA

更新日期：1989-10-01 00:00:00

abstract：:The API 20S system (Analytab Products, Plainview, N.Y.) was evaluated for the rapid identification of multiply antibiotic-resistant aerobic diphtheroids. Sixty-eight clinical isolates of multiply resistant Centers for Disease Control group JK and group D2 corynebacteria had API 20S profiles which were clearly differen...

journal_title：Journal of clinical microbiology

authors： Kelly MC,Smith ID,Anstey RJ,Thornley JH,Rennie RP

更新日期：1984-02-01 00:00:00

abstract：:A rapid and sensitive PCR assay for the detection of Candida albicans DNA in serum was established. DNA from human serum samples was purified using the QIAamp blood kit, which proved to be a fast and simple method for isolating minute amounts of Candida DNA from clinical specimens for diagnosis of invasive candidiasis...

journal_title：Journal of clinical microbiology

authors： Wahyuningsih R,Freisleben HJ,Sonntag HG,Schnitzler P

更新日期：2000-08-01 00:00:00

abstract：:We hypothesized that diversion of the first milliliter of venipuncture blood-the initial specimen diversion technique (ISDT)-would eliminate incompletely sterilized fragments of skin from the culture specimen and significantly reduce our blood culture contamination rate (R). We studied our hypothesis prospectively beg...

journal_title：Journal of clinical microbiology

authors： Patton RG,Schmitt T

更新日期：2010-12-01 00:00:00

abstract：:Sodium polyanethole sulfonate (SPS; trade name, Liquoid) is a constituent in culture media used to grow bacteria from blood samples from patients suspected of bacteremia. SPS prevents the killing of bacteria by innate cellular and humoral factors. We analyzed the effect of SPS on the three complement activation pathwa...

journal_title：Journal of clinical microbiology

authors： Palarasah Y,Skjoedt MO,Vitved L,Andersen TE,Skjoedt K,Koch C

更新日期：2010-03-01 00:00:00

abstract：:A loop-mediated isothermal amplification (LAMP) method for the rapid detection of serotype 1 Marek's disease virus (MDV) was developed. The method used a set of three pairs of primers to amplify the MEQ gene for detecting serotype 1 MDV. The MDV LAMP method did not cross-react with serotype 2 and serotype 3, nor did t...

journal_title：Journal of clinical microbiology

authors： Angamuthu R,Baskaran S,Gopal DR,Devarajan J,Kathaperumal K

更新日期：2012-03-01 00:00:00

abstract：:Nineteen reference and 156 clinical strains of the genus Nocardia belonging to 12 taxonomic groups were studied for restriction fragment length polymorphism (RFLP) by using an amplified 439-bp segment of the 65-kDa heat shock protein gene. Of 30 restriction endonucleases, digestion with MspI and then digestion with Bs...

journal_title：Journal of clinical microbiology

authors： Steingrube VA,Brown BA,Gibson JL,Wilson RW,Brown J,Blacklock Z,Jost K,Locke S,Ulrich RF,Wallace RJ Jr

更新日期：1995-12-01 00:00:00

abstract：:A reverse transcriptase PCR (RT-PCR) was used for rapid determination of the hemagglutinin (HA) cleavage site sequence, a marker for the virulence potential of avian influenza viruses. When applied to specimens from chickens experimentally infected with either a virulent or an avirulent virus, RT-PCR uniformly detecte...

journal_title：Journal of clinical microbiology

authors： Horimoto T,Kawaoka Y

更新日期：1995-03-01 00:00:00

abstract：:First-void urine (FVU) is the preferred specimen for the diagnosis of urogenital Chlamydia trachomatis infection in men. We have developed FirstBurst, a urine collection device that collects the first 4 to 5 ml of FVU and yields a specimen with a sixfold higher C. trachomatis organism load than the regular urine cup b...

journal_title：Journal of clinical microbiology

authors： Wisniewski CA,White JA,Michel CE,Mahilum-Tapay L,Magbanua JP,Nadala EC Jr,Barber PJ,Goh BT,Lee HH

更新日期：2008-04-01 00:00:00

abstract：:In the present study, we compared typed F. nucleatum strains with isolates from various human periodontal diseases, canines, and a Macaca mulatta monkey. All isolates displayed biochemical reactions similar to those of the typed strains in the API 20A system (Analytab Products, Plainville, N.Y.). The human and monkey ...

journal_title：Journal of clinical microbiology

authors： Vincent JW,Falkler WA Jr,Craig JA

更新日期：1983-04-01 00:00:00

abstract：:We report disseminated adenovirus (ADV) infection in four adult cancer patients presenting with focal pulmonary consolidation. In all cases, ADV was recovered from respiratory specimens and ADV viremia (>1 × 10(5) copies/ml) was determined by a quantitative PCR assay. Despite antiviral therapy, 3 (75%) patients died. ...

journal_title：Journal of clinical microbiology

authors： Lee YJ,Palomino-Guilen P,Babady NE,Lamson DM,St George K,Tang YW,Papanicolaou GA

更新日期：2014-01-01 00:00:00

abstract：:Procedures for differentiation between wild and vaccine-derived strains of poliovirus are required, particularly in countries where wild and vaccine-related strains coexist. For this differentiation, we tested the method of Inouye and Hondo (S. Inouye and R. Hondo, Arch. Virol. 129:311-316, 1993) for discrimination of...

journal_title：Journal of clinical microbiology

authors： Takeda N,Sakae K,Agboatwalla M,Isomura S,Hondo R,Inouye S

更新日期：1994-01-01 00:00:00

abstract：:We have recently developed a PCR assay for detection of Mycobacterium spp. at the genus level based on the Cobas Amplicor platform. The sensitivities for smear-positive and smear-negative specimens were found to be 100% and 47.9%, respectively. The specificity was 97.7%, the positive predictive value 84.6%, and the ne...

journal_title：Journal of clinical microbiology

authors： Peter-Getzlaff S,Lüthy J,Voit A,Bloemberg GV,Böttger EC

更新日期：2010-11-01 00:00:00

abstract：:In order to differentiate species within the Borrelia burgdorferi sensu lato complex, LightCyler PCR and melting-curve analysis of the amplicons of two genes with intraspecies variability, the p66 gene and the recA gene, were performed. It was demonstrated that nested LightCycler PCR amplification of p66 is more sensi...

journal_title：Journal of clinical microbiology

authors： Mommert S,Gutzmer R,Kapp A,Werfel T

更新日期：2001-07-01 00:00:00

abstract：:Sixteen metabolically deficient dwarf-colony mutants of Escherichia coli were isolated from urine culture and represented about 2% of all E. coli isolated during the same period. In 14 cases, mutants were isolated from debilitated patients: elderly persons or patients in the terminal stages of a chronic disease. In 15...

journal_title：Journal of clinical microbiology

authors： Borderon E,Horodniceanu T

更新日期：1978-12-01 00:00:00

abstract：:We evaluated nasopharyngeal carriage of Streptococcus pneumoniae (pneumococci) in nine Alaskan communities and used an algorithm combining microbiologic, serologic, and sequential multiplex PCR (MP-PCR) techniques to serotype the isolates. After microbiological identification as pneumococci, isolates (n = 1,135) were ...

journal_title：Journal of clinical microbiology

authors： Miernyk K,Debyle C,Harker-Jones M,Hummel KB,Hennessy T,Wenger J,Rudolph K

更新日期：2011-09-01 00:00:00

abstract：:An indirect enzyme-linked immunosorbent assay was used for the screening of horse sera from The Gambia for antibodies against African horse sickness virus (AHSV). The AHSV antigen used for coating was semipurified according to the method of Manning and Chen (Curr. Microbiol. 4:381, 1980); control mock-infected Vero ce...

journal_title：Journal of clinical microbiology

authors： Staeuber N,Fye B,Zinsstag J,McCullough KC

更新日期：1993-08-01 00:00:00

abstract：:A novel reverse primer (GLM MR1) was designed for detection of the glmM gene in Helicobacter pylori by PCR. The percentage of amplification in clinical isolates using GLM MR1 was 100% for detection of the glmM gene and 86.36% for the ureA gene. The primer designed is useful for the identification of H. pylori. ...

journal_title：Journal of clinical microbiology

authors： Espinoza MG,Vazquez RG,Mendez IM,Vargas CR,Cerezo SG

更新日期：2011-04-01 00:00:00

abstract：:A modified indirect hemagglutination test for the detection of treponemal antibodies was developed for use with finger-prick blood. By using paired serum and absorbed finger-prick blood from 58 patients from an area previously endemic for yaws and 12 patients without yaws, the modified hemagglutination test was compar...

journal_title：Journal of clinical microbiology

authors： Backhouse JL,Lee MH,Nesteroff SI,Hudson BJ,Hamilton PA

更新日期：1992-03-01 00:00:00

abstract：:We present the first case of severe pneumonia possibly caused by Leptotrichia species with oral bacteria. This was found in a healthy but elderly subject whose bronchoalveolar lavage fluid was analyzed by 16S rRNA gene sequencing analysis. The combination of this method and microscopic observation provided useful info...

journal_title：Journal of clinical microbiology

authors： Kawanami T,Fukuda K,Yatera K,Kido T,Yoshii C,Taniguchi H,Kido M

更新日期：2009-02-01 00:00:00

abstract：:Blood culture bottles with antimicrobial removal systems are recommended for patients who develop fever while on antibiotics. This study compared the ability of Becton Dickinson (Sparks, MD) BACTEC PLUS bottles and bioMerieux (Durham, NC) BacT/Alert FA bottles to effectively remove vancomycin, cefoxitin, ceftriaxone, ...

journal_title：Journal of clinical microbiology

authors： Flayhart D,Borek AP,Wakefield T,Dick J,Carroll KC

更新日期：2007-03-01 00:00:00

abstract：:This study evaluated the performance of Directigen FluA combined with a 3-day flu screening culture for the detection of influenza virus. This abbreviated protocol was a useful and effective tool and resulted in a substantial reduction in time, effort, and money spent, while not compromising sensitivity of influenza v...

journal_title：Journal of clinical microbiology

authors： Newton DW,Mellen CF,Baxter BD,Atmar RL,Menegus MA

更新日期：2002-11-01 00:00:00

abstract：:The activity of three aminoglycosides and six beta-lactam antibiotics on strains of Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, Staphylococcus aureus, and enterococci was studied. The minimal inhibitory concentrations (MICs), the minimal bactericidal concentrations (MBCs), and t...

journal_title：Journal of clinical microbiology

authors： Lorian V,Atkinson BA

更新日期：1982-07-01 00:00:00

abstract：:The API 20S system (Analytab Products, Plainview, N.Y.) and the AutoMicrobic Gram-Positive Identification system (GPI; Vitek Systems, Hazelwood, Mo.) were evaluated for their capacity to identify the non-beta-hemolytic streptococci and aerococci to the species level. The 20S system identified 86% (six of seven strains...

journal_title：Journal of clinical microbiology

authors： Facklam R,Bosley GS,Rhoden D,Franklin AR,Weaver N,Schulman R

更新日期：1985-04-01 00:00:00

abstract：:Invasive meningococcal disease is mainly caused by Neisseria meningitidis serogroups A, B, C, X, W, and Y. The serogroup is typically determined by slide agglutination serogrouping (SASG) and real-time PCR (RT-PCR). We describe a whole-genome sequencing (WGS)-based method to characterize the capsule polysaccharide syn...

journal_title：Journal of clinical microbiology

authors： Marjuki H,Topaz N,Rodriguez-Rivera LD,Ramos E,Potts CC,Chen A,Retchless AC,Doho GH,Wang X

更新日期：2019-02-27 00:00:00

abstract：:Serological tests to detect Trypanosoma cruzi antibodies have been used for screening blood donors, for epidemic studies, and for diagnosis of probably infected persons. Among different tests, the enzyme-linked immunosorbent assay (ELISA) with total, semipurified, or synthetic antigens has been widely used, mainly due...

journal_title：Journal of clinical microbiology

authors： Ferreira AW,Belem ZR,Lemos EA,Reed SG,Campos-Neto A

更新日期：2001-12-01 00:00:00

abstract：:The volume of blood sampled for blood culture determines its sensitivity. We measured low mean blood volumes in submitted aerobic (8.38 ± 3.88 ml) and anaerobic (7.16 ± 3.83 ml) blood culture bottles. Educational seminars were held for phlebotomy teams, and renewed measurements thereafter revealed significantly higher...

journal_title：Journal of clinical microbiology

authors： van Ingen J,Hilt N,Bosboom R

更新日期：2013-03-01 00:00:00

abstract：:The continuous spread of community-acquired methicillin-resistant Staphylococcus aureus (caMRSA) and the introduction of these highly virulent isolates into hospitals represent increasing threats. The timely recognition of caMRSA strains is crucial for infection control purposes. Thus, we developed a PCR-based assay f...

journal_title：Journal of clinical microbiology

authors： Strommenger B,Braulke C,Pasemann B,Schmidt C,Witte W

更新日期：2008-02-01 00:00:00

abstract：:A multilocus sequence typing (MLST) scheme was developed for Klebsiella pneumoniae. Sequences of seven housekeeping genes were obtained for 67 K. pneumoniae strains, including 19 ceftazidime- and ciprofloxacin-resistant isolates. Forty distinct allelic profiles were identified. MLST data were validated against ribotyp...

journal_title：Journal of clinical microbiology

authors： Diancourt L,Passet V,Verhoef J,Grimont PA,Brisse S

更新日期：2005-08-01 00:00:00

abstract：:A collection of 147 isolates of Burkholderia pseudomallei, B. mallei, and B. thailandensis was characterized by multilocus sequence typing (MLST). The 128 isolates of B. pseudomallei, the causative agent of melioidosis, were obtained from diverse geographic locations, from humans and animals with disease, and from the...

journal_title：Journal of clinical microbiology

authors： Godoy D,Randle G,Simpson AJ,Aanensen DM,Pitt TL,Kinoshita R,Spratt BG

更新日期：2003-05-01 00:00:00