Detection and identification of Mycobacterium spp. in clinical specimens by combining the Roche Cobas Amplicor Mycobacterium tuberculosis assay with Mycobacterium genus detection and nucleic acid sequencing.

abstract：:Anthopsis deltoidea was found to be the cause of an olecranon bursitis in a 79-year-old golfer. Serial histological sections of the olecranon bursa showed faintly stained, brown-walled, septate, hyphal elements in the centers of the necrotic debris. The combination of bursectomy and flucytosine treatment cured the inf...

journal_title：Journal of clinical microbiology

authors： Kwon-Chung KJ,Droller DD

更新日期：1984-08-01 00:00:00

abstract：:The development of a rhinovirus (RV)-RNA-specific reverse transcription (RT)-PCR assay is complicated by the close homology between the RV and enterovirus (EV) genomes in the highly conserved 5'-noncoding region, which is chosen for primer design in most RT-PCR assays. We have developed a sensitive, rapid, and RV-spec...

journal_title：Journal of clinical microbiology

authors： Steininger C,Aberle SW,Popow-Kraupp T

更新日期：2001-01-01 00:00:00

abstract：:A simple procedure for the selective isolation of the protective species-specific protein antigens (SPAs) of Rickettsia typhi and Rickettsia prowazekii was developed to permit use of the SPAs in the immunodiagnosis and immunoprophylaxis of typhus infections. Although the SPAs were readily extracted from lysozyme- or d...

journal_title：Journal of clinical microbiology

authors： Dasch GA

更新日期：1981-09-01 00:00:00

abstract：:Chlamydia trachomatis was recoved from 20% (36/180) of women attending a venereal disease clinic. All infected women had chlamydial antibodies in their serum and cervical secretions. However, the background rates of chlamydial antibody in chlamydia-negative women were very high. Measurement of antibodies in serum (com...

journal_title：Journal of clinical microbiology

authors： Schachter J,Cles L,Ray R,Hines PA

更新日期：1979-11-01 00:00:00

abstract：:A Campylobacter-like organism was isolated from the ilea of normal hamsters. The organism was isolated from an ileal homogenate which was passed through a filter (0.65-micron pore size) and cultured on blood-agar plates in a microaerophilic atmosphere at 37 degrees C. Pinpoint translucent colonies were first observed ...

journal_title：Journal of clinical microbiology

authors： Stills HF Jr,Hook RR Jr,Kinden DA

更新日期：1989-11-01 00:00:00

abstract：:A Swedish bovine and a Dutch human phage set for coagulase-negative staphylococci were used to phage type coagulase-negative staphylococci isolated from bovine milk from Minnesota dairy herds. A comparison was also made of the deoxyribonuclease activity of coagulase-negative staphylococci isolated from bovine milk in ...

journal_title：Journal of clinical microbiology

authors： Holmberg O,Lyon RH,Price D,Farnsworth RJ

更新日期：1979-09-01 00:00:00

abstract：:MARC-145 cell line is commonly used to isolate porcine reproductive and respiratory syndrome virus (PRRSV) for diagnostics, research and vaccine production, but it yields frustratingly low success rates of virus isolation (VI). ZMAC cell line, derived from porcine alveolar macrophages, has become available but its uti...

journal_title：Journal of clinical microbiology

authors： Yim-Im W,Huang H,Park J,Wang C,Calzada G,Gauger P,Harmon K,Main R,Zhang J

更新日期：2020-12-02 00:00:00

abstract：:In a multicenter study, 113 blood samples from 19 organ transplant patients were analyzed for cytomegalovirus by the pp65 antigenemia assay and a quantitative DNA hybridization assay. Overall, there was 84% agreement among the results obtained by the two tests. Fifteen of 16 episodes of active infection were detected ...

journal_title：Journal of clinical microbiology

authors： Bossart W,Bienz K,Wunderli W

更新日期：1997-12-01 00:00:00

abstract：:A previously described microbroth kinetic system (J. Meletiadis, J. F. Meis, J. W. Mouton, and P. E. Verweij, J. Clin. Microbiol. 39:478-484, 2001) based on continuous monitoring of changes in the optical density of fungal growth was used to describe turbidimetric growth curves of different filamentous fungi in the pr...

journal_title：Journal of clinical microbiology

authors： Meletiadis J,te Dorsthorst DT,Verweij PE

更新日期：2003-10-01 00:00:00

abstract：:We report the recovery of Histoplasma capsulatum from blood specimens cultured for Mycobacterium sp. in BACTEC 13A radiometric medium. H. capsulatum was recovered from six of eight blood specimens submitted for mycobacterial cultures from five human immunodeficiency virus-positive individuals. Initial positive metabol...

journal_title：Journal of clinical microbiology

authors： Merz WG,Kodsy S,Merz CS

更新日期：1992-01-01 00:00:00

abstract：:The first outbreak caused by vancomycin-resistant enterococci of the VanB phenotype in Poland was analyzed. It occurred in a single ward of a Warsaw hospital which is a specialized center for the treatment of hematological disorders. Between July 1999 and February 2000, 11 patients in the ward were found to be infecte...

journal_title：Journal of clinical microbiology

authors： Kawalec M,Gniadkowski M,Zaleska M,Ozorowski T,Konopka L,Hryniewicz W

更新日期：2001-05-01 00:00:00

abstract：:The cellular fatty acid composition of Legionella feeleii was determined by capillary gas-liquid chromatography, and the ubiquinone content was determined by reverse-phase high-pressure liquid chromatography. As in other Legionella species, this new species is characterized by relatively large amounts of branched-chai...

journal_title：Journal of clinical microbiology

authors： Moss CW,Bibb WF,Karr DE,Guerrant GO,Lambert MA

更新日期：1983-10-01 00:00:00

abstract：:Classical microbiological diagnosis of human brucellosis is time-consuming, hazardous, and subject to variable interpretation. Intact-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) was evaluated for the routine identification of Brucella spp. Analysis of mass peak patt...

journal_title：Journal of clinical microbiology

authors： Karger A,Melzer F,Timke M,Bettin B,Kostrzewa M,Nöckler K,Hohmann A,Tomaso H,Neubauer H,Al Dahouk S

更新日期：2013-09-01 00:00:00

abstract：:Yarrowia lipolytica is a weakly pathogenic yeast that is rarely isolated from the blood. We observed transient recurrent catheter-related fungemia attributable to this organism in a leukemic patient. The fungemia and accompanying fever subsided spontaneously. The data suggest that it might be possible to withhold spec...

journal_title：Journal of clinical microbiology

authors： Chang CL,Park TH,Lee EY,Lim YT,Son HC

更新日期：2001-03-01 00:00:00

abstract：:This study reports the first isolation of Candida dubliniensis from North American Indians. Of 39 healthy human hosts sampled, two had C. dubliniensis. Genotypic analysis identified polymorphisms in these strains and differences from two reference strains. Our results suggest that yeast populations from indigenous com...

journal_title：Journal of clinical microbiology

authors： Montour L,Tey R,Xu J

更新日期：2003-07-01 00:00:00

abstract：:In a prospective observational study of bacteremic patients we ascertained the influence of different parts of culture results on the correctness of empirical antibiotic therapy. Ninety-three bacteremic patients requiring antibiotic treatment were included. Patients who had consultations with an infectious disease con...

journal_title：Journal of clinical microbiology

authors： Kerremans JJ,Verbrugh HA,Vos MC

更新日期：2012-06-01 00:00:00

abstract：:We have developed a sensitive assay for Treponema pallidum subsp. pallidum (T. pallidum), the agent of veneral syphilis, based upon the polymerase chain reaction (PCR). A 658-bp portion of the gene encoding the 47-kDa membrane immunogen was amplified, and the PCR products were probed by DNA-DNA hybridization with a 49...

journal_title：Journal of clinical microbiology

authors： Burstain JM,Grimprel E,Lukehart SA,Norgard MV,Radolf JD

更新日期：1991-01-01 00:00:00

abstract：:To improve the understanding of the relative importance of serotypes of rotavirus in dehydrating diarrhea, we examined the correlation of clinical characteristics and disease severity with serotype in 2,441 diarrheal episodes among children younger than 2 years of age in rural Bangladesh. Of 764 rotavirus-associated e...

journal_title：Journal of clinical microbiology

authors： Bern C,Unicomb L,Gentsch JR,Banul N,Yunus M,Sack RB,Glass RI

更新日期：1992-12-01 00:00:00

abstract：:The enzyme-linked immunosorbent assay (ELISA) has been proposed as an alternative serologic diagnostic test to the indirect immunofluorescence assay (IFA) for scrub typhus. Here, we systematically determine the optimal sample dilution and cutoff optical density (OD) and estimate the accuracy of IgM ELISA using Bayesia...

journal_title：Journal of clinical microbiology

authors： Blacksell SD,Lim C,Tanganuchitcharnchai A,Jintaworn S,Kantipong P,Richards AL,Paris DH,Limmathurotsakul D,Day NPJ

更新日期：2016-06-01 00:00:00

abstract：:Forty human clinical Mycobacterium avium-M. intracellulare complex strains isolated in Greece were characterized to the species level by PCR with three sets of primers specific for one or both species. M. avium predominated in both human immunodeficiency virus-positive and -negative patients, but the frequency of M. i...

journal_title：Journal of clinical microbiology

authors： Kyriakopoulos AM,Tassios PT,Matsiota-Bernard P,Marinis E,Tsaousidou S,Legakis NJ

更新日期：1997-11-01 00:00:00

abstract：:The current diagnosis of West Nile virus (WNV) infection is primarily based on serology, since molecular identification of WNV RNA is unreliable due to the short viremia and absence of detectable virus in cerebrospinal fluid (CSF). Recent studies have shown that WNV RNA can be detected in urine for a longer period and...

journal_title：Journal of clinical microbiology

authors： Lustig Y,Mannasse B,Koren R,Katz-Likvornik S,Hindiyeh M,Mandelboim M,Dovrat S,Sofer D,Mendelson E

更新日期：2016-09-01 00:00:00

abstract：:An 840-bp fragment of the 18S rRNA gene was used to identify Cryptosporidium spp. recovered from human immunodeficiency virus (HIV)-infected and -uninfected patients from Kenya, Malawi, Brazil, the United Kingdom, and Vietnam. Initial identification was by Ziehl-Neelsen acid-fast staining. Confirmation was by nested P...

journal_title：Journal of clinical microbiology

authors： Gatei W,Greensill J,Ashford RW,Cuevas LE,Parry CM,Cunliffe NA,Beeching NJ,Hart CA

更新日期：2003-04-01 00:00:00

abstract：:Simple enzymatic assays to detect heat-labile enterotoxins whose modes of action are similar to that of cholera toxin were evaluated. The assays are performed by using an artificial substrate, diethylamino benzylidine-aminoguanidine, which is an ADP-ribose acceptor. The product, formed in the presence of NAD+, can be ...

journal_title：Journal of clinical microbiology

authors： Narayanan J,Hartman PA,Graves DJ

更新日期：1989-11-01 00:00:00

abstract：:A qualitative multiplex reverse transcription (RT)-PCR and liquid hybridization assay for the detection of human enteroviruses, rhinoviruses, parechoviruses, and Aichi virus was developed. Furthermore, a separate assay for the recognition of hepatitis A virus was established to complement the test pattern so that all ...

journal_title：Journal of clinical microbiology

authors： Jokela P,Joki-Korpela P,Maaronen M,Glumoff V,Hyypiä T

更新日期：2005-03-01 00:00:00

abstract：:An evaluation of the serotyping system for Proteus rettgeri has been performed through its application to 561 human isolates of this species. Of these, 545 were typable and could be separated into 58 O serotypes. A group of isolates from one hospital, and clinically implicated in cross-infections, was found to belong ...

journal_title：Journal of clinical microbiology

authors： Penner JL,Hinton NA,Hennessy JN

更新日期：1976-04-01 00:00:00

abstract：:We report transmission of an azole-resistant, isogenic strain of Candida albicans in a human immunodeficiency virus (HIV)-infected family of two children with symptomatic oropharyngeal candidiasis and a mother with asymptomatic colonization over a 5-year period. These findings were confirmed by three different molecul...

journal_title：Journal of clinical microbiology

authors： Müller FM,Kasai M,Francesconi A,Brillante B,Roden M,Peter J,Chanock SJ,Walsh TJ

更新日期：1999-10-01 00:00:00

abstract：:A heminested inverse PCR (HIP) for the amplification of sequences flanking the Mycobacterium tuberculosis insertion sequence IS6110 has been developed. The method depends upon primers that anneal to IS6110 at sites between its 5' end and the closest BsrFI site. The accuracy of HIP was demonstrated by the amplification...

journal_title：Journal of clinical microbiology

authors： Patel S,Wall S,Saunders NA

更新日期：1996-07-01 00:00:00

abstract：:Detection of Staphylococcus aureus isolates with intermediate vancomycin susceptibility (VISA) and heteroresistance (hVISA) remains problematic. The population analysis profile/area under the curve (PAP/AUC) is the gold standard but is cumbersome. We compared the performance of two Etest screening methods (macromethod...

journal_title：Journal of clinical microbiology

authors： Riederer K,Shemes S,Chase P,Musta A,Mar A,Khatib R

更新日期：2011-06-01 00:00:00

abstract：:A chemically defined medium that simulates female genital tract secretions was developed for the growth of the vaginal microflora. Qualitative and quantitative studies of the growth of predominant components of the vaginal microflora indicated that all vaginal isolates tested were able to grow in this defined medium. ...

journal_title：Journal of clinical microbiology

authors： Geshnizgani AM,Onderdonk AB

更新日期：1992-05-01 00:00:00

abstract：:Although TT virus (TTV) was isolated from a cryptogenic posttransfusion hepatitis patient, its pathogenic role remains unclear. It has been reported that the majority of the healthy population is infected with TTV. To elucidate the differences between TTV infection in patients with liver diseases and TTV infection in ...

journal_title：Journal of clinical microbiology

authors： Kato T,Mizokami M,Mukaide M,Orito E,Ohno T,Nakano T,Tanaka Y,Kato H,Sugauchi F,Ueda R,Hirashima N,Shimamatsu K,Kage M,Kojiro M

更新日期：2000-01-01 00:00:00