Comparative evaluation of the API 20S and AutoMicrobic gram-positive identification systems for non-beta-hemolytic streptococci and aerococci.

abstract：:Rodent models have been developed to study the pathogenesis of diseases caused by Helicobacter pylori, as well as by other gastric and intestinal Helicobacter spp., but some murine enteric Helicobacter spp. cause hepatobiliary and intestinal tract diseases in specific inbred strains of laboratory mice. To identify the...

journal_title：Journal of clinical microbiology

authors： Nilsson HO,Ouis IS,Stenram U,Ljungh A,Moran AP,Wadström T,Al-Soud WA

更新日期：2004-08-01 00:00:00

abstract：:The duration of mumps virus RNA detection was studied during a mumps outbreak in a highly vaccinated university population. Seven of the eight reverse transcription-PCR-positive specimens were collected during the first 3 days of parotitis, suggesting that viral shedding is minimal after the first 3 days of symptoms. ...

journal_title：Journal of clinical microbiology

authors： Bitsko RH,Cortese MM,Dayan GH,Rota PA,Lowe L,Iversen SC,Bellini WJ

更新日期：2008-03-01 00:00:00

abstract：:Repetitive sequence-based PCR (rep-PCR) and amplified fragment length polymorphism (AFLP) were used to characterize a sample of 43 field isolates and 4 attenuated vaccine strains of Pasteurella multocida recovered from multiple avian species. Both rep-PCR and AFLP assays were rapid and reproducible, with high indices ...

journal_title：Journal of clinical microbiology

authors： Amonsin A,Wellehan JF,Li LL,Laber J,Kapur V

更新日期：2002-08-01 00:00:00

abstract：:Fifty staphylococcal strains that produced toxic shock syndrome (TSS) toxin 1 and that were isolated from patients with TSS were characterized. One strain had more properties that were characteristic of Staphylococcus hyicus than of Staphylococcus aureus. Forty-four strains had the same properties or differed in only ...

journal_title：Journal of clinical microbiology

authors： Aliu B,Bergdoll MS

更新日期：1988-11-01 00:00:00

abstract：:Thirty-three strains of Cryptococcus neoformans were isolated from clinical specimens, including specimens from AIDS patients in Brazil, and were classified into two serotypes; we detected 31 and 2 strains of serotypes A and B, respectively. Random amplified polymorphic DNA (RAPD) fingerprint pattern analyses of these...

journal_title：Journal of clinical microbiology

authors： Aoki FH,Imai T,Tanaka R,Mikami Y,Taguchi H,Nishimura NF,Nishimura K,Miyaji M,Schreiber AZ,Branchini ML

更新日期：1999-02-01 00:00:00

abstract：:In Brazil, Salmonella enterica serovar Infantis resistant to various antimicrobials, including cephalosporins, has been identified as an etiological agent of severe gastroenteritis in hospitalized children since 1994. In this study, 35 serovar Infantis strains, isolated from children admitted to four different Rio de ...

journal_title：Journal of clinical microbiology

authors： Fonseca EL,Mykytczuk OL,Asensi MD,Reis EM,Ferraz LR,Paula FL,Ng LK,Rodrigues DP

更新日期：2006-08-01 00:00:00

abstract：:The taxonomic status and structure of Streptococcus dysgalactiae have been the object of much confusion. Bacteria belonging to this species are usually referred to as Lancefield group C or group G streptococci in clinical settings in spite of the fact that these terms lack precision and prevent recognition of the exac...

journal_title：Journal of clinical microbiology

authors： Jensen A,Kilian M

更新日期：2012-01-01 00:00:00

abstract：:A recombinant gene fusion protein composed of an Escherichia coli heat-stable enterotoxin (STa) peptide epitope fused to the amino end of the cholera toxin B subunit was used to detect STa produced by clinical isolates of enterotoxigenic E. coli (STa-ETEC) by a single monoclonal antibody-based inhibition GM1 enzyme-li...

journal_title：Journal of clinical microbiology

authors： Sanchez J,Holmgren J,Svennerholm AM

更新日期：1990-10-01 00:00:00

abstract：:A multicenter cross-sectional survey was conducted comparing a commercially available chlamydial optical immunoassay (OIA) to the chlamydial ligase chain reaction (LCR). Endocervical samples from 415 outpatients visiting clinics from three hospitals in South Kalimantan, Indonesia, were evaluated. Relative to the LCR, ...

journal_title：Journal of clinical microbiology

authors： Widjaja S,Cohen S,Brady WE,O'reilly K,Susanto,Wibowo A,Cahyono,Graham RR,Porter KR

更新日期：1999-12-01 00:00:00

abstract：:Animal bite wounds are often infected with bacteria from the animal's oral flora. We report what we believe to be the first case of bacteremia with Bacteroides pyogenes resulting from an infected cat bite. ...

journal_title：Journal of clinical microbiology

authors： Madsen IR,Justesen US

更新日期：2011-08-01 00:00:00

abstract：:We have previously reported that the pCS20 PCR detection assay for Cowdria ruminantium, the causative agent of heartwater disease of ruminants, is more sensitive than xenodiagnosis and the pCS20 DNA probe for the detection of infection in the vector Amblyomma ticks. Here, we further assessed the reliability of the PCR...

journal_title：Journal of clinical microbiology

authors： Peter TF,Barbet AF,Alleman AR,Simbi BH,Burridge MJ,Mahan SM

更新日期：2000-04-01 00:00:00

abstract：:Results of serotyping 966 clinical isolates of Pseudomonas aeruginosa showed that 72% agglutinated specifically in one or another of the 16 typing antisera, but 28% agglutinated in two or more and often in as many as 10 antisera; this polyagglutinability correlated with a high incidence of cross-reactivity among the a...

journal_title：Journal of clinical microbiology

authors： Duncan NH,Hinton NA,Penner JL,Duncan IB

更新日期：1976-08-01 00:00:00

abstract：:A species-specific molecular beacon real-time PCR assay was developed for rapid diagnosis of Exserohilum rostratum infection. As low as 100 fg of E. rostratum DNA can be reliably detected in the presence of 50 ng of human DNA, with a dynamic linear quantification range from 20 ng to 200 fg. ...

journal_title：Journal of clinical microbiology

authors： Zhao Y,Petraitiene R,Walsh TJ,Perlin DS

更新日期：2013-03-01 00:00:00

abstract：:The protease phenotypes expressed by isolates of Pseudomonas aeruginosa from cystic fibrosis (CF) patients were evaluated. The majority of isolates tested produced elastase (65%) or alkaline protease (64%) or both. The mucoid phenotype expressed by many CF isolates of P. aeruginosa did not absolutely restrict the expr...

journal_title：Journal of clinical microbiology

authors： Jagger KS,Bahner DR,Warren RL

更新日期：1983-01-01 00:00:00

abstract：:Mycobacterium bovis infection in wildlife and feral species is a potential source of infection for livestock and a threat to protected and endangered species. The aim of this study was to identify Spanish wild animal species infected with M. bovis through bacteriological culture and spacer oligonucleotide typing (spol...

journal_title：Journal of clinical microbiology

authors： Aranaz A,De Juan L,Montero N,Sánchez C,Galka M,Delso C,Alvarez J,Romero B,Bezos J,Vela AI,Briones V,Mateos A,Domínguez L

更新日期：2004-06-01 00:00:00

abstract：:The polymerase chain reaction (PCR) has many potential applications in the field of nucleic acid diagnostics. In particular, it has been successfully applied to the detection of pathogens present in low copy numbers such as the human immunodeficiency virus type 1. Here we describe a time-resolved fluorescence-based hy...

journal_title：Journal of clinical microbiology

authors： Dahlén PO,Iitiä AJ,Skagius G,Frostell A,Nunn MF,Kwiatkowski M

更新日期：1991-04-01 00:00:00

abstract：:PCR-single-strand conformation polymorphism (PCR-SSCP) analysis is a rapid and convenient technique for the detection of mutations and allelic variants. We have adapted this technique for the identification of bacteria by PCR with fluorescein-labeled primers chosen from the conserved regions of the 16S rRNA gene flank...

journal_title：Journal of clinical microbiology

authors： Widjojoatmodjo MN,Fluit AC,Verhoef J

更新日期：1995-10-01 00:00:00

abstract：:Recent studies have speculated on the possible role of the mother in transmitting Helicobacter pylori infection to their children. In an attempt to either prove or disprove this supposition, we investigated the rates of infection of children born to H. pylori-positive mothers from birth to 5 years of age using serolog...

journal_title：Journal of clinical microbiology

authors： Konno M,Fujii N,Yokota S,Sato K,Takahashi M,Sato K,Mino E,Sugiyama T

更新日期：2005-05-01 00:00:00

abstract：:By an immunomagnetic-bead (IMB) separation technique, isolation of Helicobacter pylori from gastrointestinal and fecal samples of gnotobiotic mice infected with the microorganism was tried. The isolation rate of H. pylori from stomach samples after IMB separation was not higher than that of direct culture of the sampl...

journal_title：Journal of clinical microbiology

authors： Osaki T,Taguchi H,Yamaguchi H,Kamiya S

更新日期：1998-01-01 00:00:00

abstract：:We developed a group B streptococcus multiplex PCR assay which allows, by direct analysis of the amplicon size, determination of the surface protein antigen genes of alpha-C protein, epsilon protein, Rib, Alp2, Alp3, and Alp4. The multiplex PCR assay offers a rapid and simple method of subtyping Streptococcus agalacti...

journal_title：Journal of clinical microbiology

authors： Creti R,Fabretti F,Orefici G,von Hunolstein C

更新日期：2004-03-01 00:00:00

abstract：:Serum samples collected from 68 patients (age, mean +/- the standard deviation [SD], 56.3 +/- 12.8 years) at admission who were subsequently molecularly diagnosed as having hepatitis E and from 2,781 individuals who were assumed not to have been recently infected with hepatitis E virus (HEV; negative controls; 52.9 +/...

journal_title：Journal of clinical microbiology

authors： Takahashi M,Kusakai S,Mizuo H,Suzuki K,Fujimura K,Masuko K,Sugai Y,Aikawa T,Nishizawa T,Okamoto H

更新日期：2005-01-01 00:00:00

abstract：:An assessment was made of the necessity of performing routine screening for Staphylococcus aureus in stool specimens. A total of 527 stool specimens were evaluated. Because of the rare incidence of staphylococcal enterocolitis and the high ($2.50) screening cost, the results of this evaluation suggest that such a scre...

journal_title：Journal of clinical microbiology

authors： Cashman JS,Smith CB,Weidner M,Matsen JM

更新日期：1984-09-01 00:00:00

abstract：:Nontuberculous mycobacterial infections caused by Mycobacterium abscessus are responsible for a range of disease manifestations from pulmonary to skin infections and are notoriously difficult to treat, due to innate resistance to many antibiotics. Previous population studies of clinical M. abscessus isolates utilized ...

journal_title：Journal of clinical microbiology

authors： Davidson RM,Hasan NA,Reynolds PR,Totten S,Garcia B,Levin A,Ramamoorthy P,Heifets L,Daley CL,Strong M

更新日期：2014-10-01 00:00:00

abstract：:Salmonella enterica subsp. enterica serovar Typhimurium var. Copenhagen isolates from a heifer-raising operation and from 11 dairy herds that had their calves contracted to the heifer-raising operation were examined for their phenotypic and genotypic characteristics. Results of the study showed that the heifer-raising...

journal_title：Journal of clinical microbiology

authors： Hegde NV,Cook ML,Wolfgang DR,Love BC,Maddox CC,Jayarao BM

更新日期：2005-08-01 00:00:00

abstract：:Five strains of an unknown, multidrug-resistant coryneform, gram-positive rod were isolated from blood, bronchial aspirate, and abscess specimens. Four of the five strains isolated were highly resistant to antimicrobial agents, including beta-lactams, aminoglycosides, macrolides, quinolones, and tetracyclines, except ...

journal_title：Journal of clinical microbiology

authors： Otsuka Y,Kawamura Y,Koyama T,Iihara H,Ohkusu K,Ezaki T

更新日期：2005-08-01 00:00:00

abstract：:The group B streptococcus (GBS) capsular polysaccharide (CPS) is an important virulence factor which is also used for GBS typing. There are 10 CPS types (Ia, Ib, and II to IX). GBS that do not phenotypically type are considered nontypeable. All genes required for CPS synthesis are found on the GBS cps operon, which co...

journal_title：Journal of clinical microbiology

authors： Alhhazmi A,Pandey A,Tyrrell GJ

更新日期：2017-09-01 00:00:00

abstract：:The appearance and persistence of immunoglobulin M (IgM) and IgG antibodies in postnatal rubella infections were studied by employing a solid-phase radioimmunoassay test. Altogether, 222 serial serum specimens from 51 patients with acute rubella infection were tested. Both IgG and IgM antibodies developed rapidly and ...

journal_title：Journal of clinical microbiology

authors： Meurman OH

更新日期：1978-01-01 00:00:00

abstract：:A production model of the semi-automated PASCO MIC-ID system (PASCO Laboratories, Wheat Ridge, Colo.) was evaluated with 122 groups or species of gram-negative bacilli, which included typical (499 cultures) and atypical (37 cultures) strains of fermenters and nonfermenters. The PASCO system identified 90.9% of 536 cul...

journal_title：Journal of clinical microbiology

authors： Rhoden DL,Schable B,Smith PB

更新日期：1987-12-01 00:00:00

abstract：:We recently developed a mass spectrometry (MS) procedure based on the detection of a serum disaccharide (MS-DS) in patients with invasive candidiasis (IC). Here, we compare the performance of MS-DS for the diagnosis of IC, invasive aspergillosis (IA), and mucormycosis (MM) with those of commercially available antigen ...

journal_title：Journal of clinical microbiology

authors： Mery A,Sendid B,François N,Cornu M,Poissy J,Guerardel Y,Poulain D

更新日期：2016-11-01 00:00:00

abstract：:Cerebrospinal fluid samples from 257 patients with suspected herpes simplex virus encephalitis were prospectively analyzed by herpes simplex virus polymerase chain reaction. The polymerase chain reaction indicated herpes simplex virus encephalitis in 9 serologically proven cases and in 14 additional patients. Increase...

journal_title：Journal of clinical microbiology

authors： Puchhammer-Stöckl E,Heinz FX,Kundi M,Popow-Kraupp T,Grimm G,Millner MM,Kunz C

更新日期：1993-01-01 00:00:00