Detection of RNA of mumps virus during an outbreak in a population with a high level of measles, mumps, and rubella vaccine coverage.

abstract：:Molecular typing has become indispensable in the detection of nosocomial transmission of bacterial pathogens and the identification of sources and routes of transmission in outbreak settings, but current methods are labor-intensive, are difficult to standardize, or have limited resolution. Whole-genome multilocus sequ...

journal_title：Journal of clinical microbiology

authors： Kluytmans-van den Bergh MF,Rossen JW,Bruijning-Verhagen PC,Bonten MJ,Friedrich AW,Vandenbroucke-Grauls CM,Willems RJ,Kluytmans JA

更新日期：2016-12-01 00:00:00

abstract：:We investigated the performance of a double-antigen sandwich recombinant enzyme immunoassay (EIA; Abbott Laboratories, North Chicago, Ill.) and compared it with that of a synthetic-peptide-based EIA (Biochem Immunosystems, Montreal, Quebec, Canada) for the detection of human immunodeficiency type 1 (HIV-1) and HIV-2 a...

journal_title：Journal of clinical microbiology

authors： Galli RA,Castriciano S,Fearon M,Major C,Choi KW,Mahony J,Chernesky M

更新日期：1996-04-01 00:00:00

abstract：:Ureaplasma parvum and Ureaplasma urealyticum are sexually transmitted, opportunistic pathogens of the human urogenital tract. There are 14 known serovars distributed between the two species. For decades, it has been postulated based upon limited data that virulence is related to serotype specificity. The results were ...

journal_title：Journal of clinical microbiology

authors： Xiao L,Paralanov V,Glass JI,Duffy LB,Robertson JA,Cassell GH,Chen Y,Waites KB

更新日期：2011-08-01 00:00:00

abstract：:Twenty-two isolates of St. Louis encephalitis (SLE) virus of various geographical origins (Brazil, Argentina, Panama, Texas, Missouri, Maryland, California, and Florida) were examined for genetic variation by the base excision sequence scanning (BESS T-scan) method. A fragment was amplified in the envelope gene with t...

journal_title：Journal of clinical microbiology

authors： Charrel RN,Lévy N,Tesh RB,Chandler LJ

更新日期：1999-06-01 00:00:00

abstract：:Pneumonia has been identified as a major cause of poor lamb survival in indigenous herds of Rocky Mountain bighorn sheep (Ovis canadensis canadensis) in central Idaho. Pasteurella haemolytica was isolated from five adult Rocky Mountain bighorn ewes captured from a free-ranging herd in central Idaho. The lambs from two...

journal_title：Journal of clinical microbiology

authors： Jaworski MD,Ward AC,Hunter DL,Wesley IV

更新日期：1993-04-01 00:00:00

abstract：:We compared the coreceptor tropism-predicting performance of a specific genotypic algorithm for HIV-1 subtype D and that of the geno2pheno algorithm with different cutoffs. The D-specific algorithm and geno2pheno with a false-positivity rate cutoff of 2.5% had the same concordance with the phenotypic determination. Th...

journal_title：Journal of clinical microbiology

authors： Dina J,Raymond S,Maillard A,Le Guillou-Guillemette H,Rodalec A,Beby-Defaux A,Giraudeau G,Vallet S,Mourez T,Payan C,Vabret A,Ruffault A,Ferre V,Izopet J,Plantier JC

更新日期：2013-09-01 00:00:00

abstract：:A novel one-step, closed-tube, loop-mediated isothermal amplification (LAMP) assay for detecting Entamoeba histolytica, one of the leading causes of morbidity in developing countries, was developed. The sensitivity of the LAMP assay is 1 parasite per reaction. A total of 130 clinical samples were analyzed, and the res...

journal_title：Journal of clinical microbiology

authors： Liang SY,Chan YH,Hsia KT,Lee JL,Kuo MC,Hwa KY,Chan CW,Chiang TY,Chen JS,Wu FT,Ji DD

更新日期：2009-06-01 00:00:00

abstract：:RNA of the newly identified human metapneumovirus (HMPV) was detected in nasopharyngeal aspirates of 11 of 63 (17.5%) young children with respiratory tract disease. Markers of infection caused by another member of the Pneumovirinae subfamily of the family Paramyxoviridae, respiratory syncytial virus (RSV), were identi...

journal_title：Journal of clinical microbiology

authors： Viazov S,Ratjen F,Scheidhauer R,Fiedler M,Roggendorf M

更新日期：2003-07-01 00:00:00

abstract：:We studied the evolution of Vibrio cholerae O1 during the 1991 to 2000 cholera epidemic in Mexico by biochemical, serological, and molecular characterization of strains collected during this period. Strains were divided into toxigenic and nontoxigenic groups according to the presence or absence of genes encoding chole...

journal_title：Journal of clinical microbiology

authors： Lizárraga-Partida ML,Quilici ML

更新日期：2009-05-01 00:00:00

abstract：:Sputum specimens received for the diagnosis of tuberculosis or other mycobacterial infections were tested by a ligase chain reaction (LCR)-based assay and acid-fast stain and culture techniques. Results from the LCR assay (Abbott LCx Mycobacterium tuberculosis [MTB] Assay) were compared to results from standard cultur...

journal_title：Journal of clinical microbiology

authors： Moore DF,Curry JI

更新日期：1998-04-01 00:00:00

abstract：:Fourth-generation human immunodeficiency virus (HIV) screening immunoassays reduce the diagnostic window between infection and diagnosis by the inclusion of HIV p24 antigen detection together with HIV antibody detection in the same test. We compared third- and fourth-generation HIV immunoassays and a dedicated HIV p24...

journal_title：Journal of clinical microbiology

authors： Speers D,Phillips P,Dyer J

更新日期：2005-10-01 00:00:00

abstract：:Yersinia enterocolitica is known to be associated with gastroenteritis in children and Reiter's syndrome in adults, but it has only rarely been the cause of primary soft tissue infections. A patient with diabetes mellitus developed a calf abscess, from which Y. enterocolitica was isolated in pure culture. Incision, dr...

journal_title：Journal of clinical microbiology

authors： Brennessel DJ,Robbins N,Hindman S

更新日期：1984-08-01 00:00:00

abstract：:The first outbreak of Candida haemulonii fungemia is described. The seven isolates from the blood of four neonates were identified by DNA sequencing of the ribosomal DNA. They were all resistant to amphotericin B, fluconazole, and itraconazole. This report highlights the emergence of C. haemulonii as an opportunistic ...

journal_title：Journal of clinical microbiology

authors： Khan ZU,Al-Sweih NA,Ahmad S,Al-Kazemi N,Khan S,Joseph L,Chandy R

更新日期：2007-06-01 00:00:00

abstract：:In a follow-up study, enterotoxigenic Escherichia coli (ETEC) infections in 145 children from two communities located in northeastern Argentina were monitored for 2 years. The occurrence of diarrhea was monitored by weekly household visits. Of 730 fecal specimens collected, 137 (19%) corresponded to diarrheal episodes...

journal_title：Journal of clinical microbiology

authors： Viboud GI,Jouve MJ,Binsztein N,Vergara M,Rivas M,Quiroga M,Svennerholm AM

更新日期：1999-09-01 00:00:00

abstract：:The genes encoding the 56-kDa polypeptides were amplified by polymerase chain reaction from the genomic DNAs of three serotypes of Rickettsia tsutsugamushi, Gilliam, Karp, and Boryong. The amplified products were cloned into expression vector pIH821, and the recombinant antigens were expressed in Escherichia coli as f...

journal_title：Journal of clinical microbiology

authors： Kim IS,Seong SY,Woo SG,Choi MS,Kang JS,Chang WH

更新日期：1993-08-01 00:00:00

abstract：:Leuconostoc species are emerging pathogens that can cause severe infections, particularly in immunocompromised patients. Using molecular methods, we identified Leuconostoc mesenteroides as the cause of a brain abscess which was successfully treated by surgery and antimicrobial treatment. This is the first report of br...

journal_title：Journal of clinical microbiology

authors： Albanese A,Spanu T,Sali M,Novegno F,D'Inzeo T,Santangelo R,Mangiola A,Anile C,Fadda G

更新日期：2006-08-01 00:00:00

abstract：:A new molecular assay, based on a rapid DNA extraction protocol, PCR, and hybridization to a specific probe in a nonradioactive microwell plate format was used to detect Mycoplasma pneumoniae in bronchoalveolar fluid specimens. The sensitivity of the assay was determined to be 10 to 100 organisms with M. pneumoniae re...

journal_title：Journal of clinical microbiology

authors： Kessler HH,Dodge DE,Pierer K,Young KK,Liao Y,Santner BI,Eber E,Roeger MG,Stuenzner D,Sixl-Voigt B,Marth E

更新日期：1997-06-01 00:00:00

abstract：:Detection of residual plasma viremia in antiretroviral therapy (ART)-suppressed HIV-infected individuals is critical for characterizing the latent reservoir and evaluating the impact of cure interventions. Ultracentrifugation-based single-copy assays are sensitive but labor intensive. Fully automated replicate testing...

journal_title：Journal of clinical microbiology

authors： Bakkour S,Deng X,Bacchetti P,Grebe E,Montalvo L,Worlock A,Stone M,Deeks SG,Richman DD,Busch MP

更新日期：2020-11-18 00:00:00

abstract：:In tests on specimens of dried blood, saliva, and urine from 55 human immunodeficiency virus (HIV)-seropositive and 55 HIV-seronegative patients, an immunoglobulin G capture enzyme immunoassay for the detection of antibodies to HIV types 1 and 2, GACELISA, gave 109 of 110, 109 of 109, and 109 of 110 correct results, r...

journal_title：Journal of clinical microbiology

authors： Thongcharoen P,Wasi C,Louisirirotchanakul S,Parry J,Connell J,Mortimer P

更新日期：1992-12-01 00:00:00

abstract：:Despite the declining rate of sexually transmitted diseases in developed countries, trichomoniasis is still one of the most common venereal infections. While diagnosis of this condition is commonly based on the microscopic wet-mount method, culture remains the most accurate single procedure for detecting the presence ...

journal_title：Journal of clinical microbiology

authors： Poch F,Levin D,Levin S,Dan M

更新日期：1996-10-01 00:00:00

abstract：:The potential clinical value of colony counts determined by the lysis-centrifugation blood culture method was studied by reviewing the records of eight patients with persistent septicemia in whom colony counts were available on at least 3 days. Colony counts of the five patients who survived decreased steadily as the ...

journal_title：Journal of clinical microbiology

authors： Whimbey E,Wong B,Kiehn TE,Armstrong D

更新日期：1984-06-01 00:00:00

abstract：:Detection and genotyping of Chlamydia trachomatis were optimized by using a polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis performed directly with crude cells of cervical scrapes. Different PCR pretreatment methods were evaluated on samples which were positive for C. trach...

journal_title：Journal of clinical microbiology

authors： Lan J,Walboomers JM,Roosendaal R,van Doornum GJ,MacLaren DM,Meijer CJ,van den Brule AJ

更新日期：1993-05-01 00:00:00

abstract：:Streptococcus mutans strains were isolated from cohorts of Brazilian nursery school children and genotyped by arbitrarily primed PCR and restriction fragment length polymorphism analysis. Of 24 children with two to five S. mutans isolates, 29% carried two or more genotypes. The presence of matching genotypes of S. mut...

journal_title：Journal of clinical microbiology

authors： Mattos-Graner RO,Li Y,Caufield PW,Duncan M,Smith DJ

更新日期：2001-06-01 00:00:00

abstract：:Isolation of Burkholderia pseudomallei currently relies on the use of Ashdown's selective agar (ASA). We designed a new selective agar (Burkholderia pseudomallei selective agar [BPSA]) to improve recovery of the more easily inhibited strains of B. pseudomallei. B. pseudomallei, Burkholderia cepacia, and Pseudomonas ae...

journal_title：Journal of clinical microbiology

authors： Howard K,Inglis TJ

更新日期：2003-07-01 00:00:00

abstract：:The complete nucleotide sequence of one of the immediate-early genes of human herpesvirus 6 variant B was determined and compared with that of variant A reported by Martin et al. (M.D. Martin, J. Nicholas, B. J. Thomson, C. Newman, and R. W. Honess, J. Virol. 65:5381-5390, 1991). While it was reported that two open re...

journal_title：Journal of clinical microbiology

authors： Yamamoto T,Mukai T,Kondo K,Yamanishi K

更新日期：1994-02-01 00:00:00

abstract：:GB virus C (GBV-C) markers were analyzed in two to three generations in three families with documented vertical transmission of GBV-C. None of the maternal grandparents had GBV-C markers, whereas the male spouses had GBV-C envelope 2 antibodies. Evidence was found for intrafamilial transmission but not for GBV-C trans...

journal_title：Journal of clinical microbiology

authors： Chen M,Fischler B,Hultgren C,Halasz R,Nemeth A,Sällberg M

更新日期：1999-12-01 00:00:00

abstract：:A PCR method for the unequivocal assignment of Haemophilus influenzae capsular type (types a to f) was developed. PCR primers were designed from capsule type-specific DNA sequences cloned from the capsular gene cluster of each of the six capsular types. PCR product was amplified only from the capsular type for which t...

journal_title：Journal of clinical microbiology

authors： Falla TJ,Crook DW,Brophy LN,Maskell D,Kroll JS,Moxon ER

更新日期：1994-10-01 00:00:00

abstract：:The detection and identification of intestinal helminths were studied retrospectively by comparing the Formalin concentration technique with the trichrome-stained smear technique. A total of 3,997 stool samples from 1,570 patients were examined by both methods. Of the 3,997 samples, 31% (1,239 of 3,997) contained helm...

journal_title：Journal of clinical microbiology

authors： Wood JC,Friedly G,de la Maza LM

更新日期：1982-12-01 00:00:00

abstract：:A sensitive and reproducible enzyme-linked immunosorbent assay (ELISA) is described for the detection of immunoglobulin M and antibodies with specifity for human cytomegalovirus (CMV) early (CMV-EA) and late (CMV-LA) antigens. The emphasis is on the production of high-quality CMV antigens, CMV-EA and CMV-LA separately...

journal_title：Journal of clinical microbiology

authors： Middeldorp JM,Jongsma J,ter Haar A,Schirm J,The TH

更新日期：1984-10-01 00:00:00

abstract：:The genetic variation within a bacterial clone (known as Shigella sonnei but in effect a clone of Escherichia coli) was examined by studying 46 clinical isolates that were epidemiologically unassociated and isolated from patients in different countries over a period of 41 years (1950 to 1991). Restriction fragment len...

journal_title：Journal of clinical microbiology

authors： Karaolis DK,Lan R,Reeves PR

更新日期：1994-03-01 00:00:00