Detection of RNA of mumps virus during an outbreak in a population with a high level of measles, mumps, and rubella vaccine coverage.


:The duration of mumps virus RNA detection was studied during a mumps outbreak in a highly vaccinated university population. Seven of the eight reverse transcription-PCR-positive specimens were collected during the first 3 days of parotitis, suggesting that viral shedding is minimal after the first 3 days of symptoms.


J Clin Microbiol


Bitsko RH,Cortese MM,Dayan GH,Rota PA,Lowe L,Iversen SC,Bellini WJ




Has Abstract


2008-03-01 00:00:00














  • Luminol-amplified chemiluminescence: a sensitive method for detecting the carrier state in chronic granulomatous disease.

    abstract::Patients with chronic granulomatous disease have a marked defect in neutrophil oxidative metabolism and microbicidal activity. Asymptomatic mothers of males with the disease can usually be identified as heterozygous carriers by intermediate leukocyte function. Most mothers of females with the disease, however, have no...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Mills EL,Rholl KS,Quie PG

    更新日期:1980-07-01 00:00:00

  • Detection of Chlamydia trachomatis and Neisseria gonorrhoeae by strand displacement amplification and relevance of the amplification control for use with vaginal swab specimens.

    abstract::Vaginal swab specimens may be preferable to cervical swab or urine specimens for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae because of the ease of specimen collection and transport. The purpose of this study was to evaluate whether vaginal swab specimens are equivalent to cervical swab specimens ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Cosentino LA,Landers DV,Hillier SL

    更新日期:2003-08-01 00:00:00

  • Molecular epidemiology of hepatitis C virus infection in an area of hyperendemicity in southern Italy: a population-based study.

    abstract::In a cohort of subjects from Italy, anti-hepatitis C virus (HCV) and HCV RNA [HCV(+) subgroup] prevalences were 24.6 and 79.6%, respectively. HCV types 1b and 2a/c accounted for 95% of infections. Adjusted alanine aminotransferase levels were higher in males than in females and in RNA-positive subjects than in RNA-neg...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Osella AR,Sonzogni L,Cavallini A,Foti L,Guerra V,Di Leo A,Mondelli MU,Misciagna G,Silini EM

    更新日期:1999-07-01 00:00:00

  • Direct fluorescent-antibody confirmation of chlamydial antigen below the detection threshold of the chlamydiazyme enzyme-linked immunosorbent assay.

    abstract::Of 4,000 endocervical specimens tested with the Chlamydiazyme enzyme-linked immunosorbent assay (Abbott Laboratories), 233 (5.8%) gave positive results (A492 above the cutoff), which were confirmed with a blocking reagent (Abbott). An additional 34 specimens (14.6%) with chlamydial antigen were detected and confirmed ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Kellogg JA,Seiple JW,Stroll ES

    更新日期:1993-06-01 00:00:00

  • Multifocal detection of multidrug-resistant Pseudomonas aeruginosa producing the PER-1 extended-spectrum beta-lactamase in Northern Italy.

    abstract::Forty-four nonreplicate clinical isolates of Pseudomonas aeruginosa that were resistant to extended-spectrum cephalosporins (ceftazidime and cefepime) and aztreonam, that putatively produced an acquired extended- spectrum beta-lactamase (ESBL), according to the results of a double-disk synergy test, and that had been ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Pagani L,Mantengoli E,Migliavacca R,Nucleo E,Pollini S,Spalla M,Daturi R,Romero E,Rossolini GM

    更新日期:2004-06-01 00:00:00

  • Comparison of serological reactions of typed Fusobacterium nucleatum strains with those of isolates from humans, canines, and a Macaca mulatta monkey.

    abstract::In the present study, we compared typed F. nucleatum strains with isolates from various human periodontal diseases, canines, and a Macaca mulatta monkey. All isolates displayed biochemical reactions similar to those of the typed strains in the API 20A system (Analytab Products, Plainville, N.Y.). The human and monkey ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Vincent JW,Falkler WA Jr,Craig JA

    更新日期:1983-04-01 00:00:00

  • Improved diagnostic medium for separation of Cryptococcus neoformans var. neoformans (serotypes A and D) and Cryptococcus neoformans var. gattii (serotypes B and C).

    abstract::A simple new agar medium containing L-canavanine, glycine, and bromthymol blue was found to give a clearer and more accurate distinction between serotype A or D (Cryptococcus neoformans var. neoformans) and serotype B or C (C. neoformans var. gattii) than creatinine-dextrose-bromthymol blue or glycine-cycloheximide-ph...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Kwon-Chung KJ,Polacheck I,Bennett JE

    更新日期:1982-03-01 00:00:00

  • Geographical heterogeneity between Far Eastern and Western countries in prevalence of the virulence plasmid, the superantigen Yersinia pseudotuberculosis-derived mitogen, and the high-pathogenicity island among Yersinia pseudotuberculosis strains.

    abstract::Yersinia pseudotuberculosis produces novel superantigenic toxins designated YPMa (Y. pseudotuberculosis-derived mitogen), YPMb, and YPMc and has a pathogenicity island termed HPI (high-pathogenicity island) and R-HPI (the right-hand part of the HPI with truncation in its left-hand part) on the chromosome. Analysis of ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Fukushima H,Matsuda Y,Seki R,Tsubokura M,Takeda N,Shubin FN,Paik IK,Zheng XB

    更新日期:2001-10-01 00:00:00

  • Quantitative PCR for human herpesviruses 6 and 7.

    abstract::A quantitative PCR assay for the detection of human herpesvirus 6 (HHV-6) (variants A and B) and HHV-7 DNAs in clinical samples was developed. The assay uses a nonhomologous internal standard (IS) for each virus that is coamplified with the wild-type target sequence in the same vial and with the same pair of primers. ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Secchiero P,Zella D,Crowley RW,Gallo RC,Lusso P

    更新日期:1995-08-01 00:00:00

  • In vitro susceptibility testing of four antibiotics against Borrelia burgdorferi: a comparison of results for the three genospecies Borrelia afzelii, Borrelia garinii, and Borrelia burgdorferi sensu stricto.

    abstract::MICs and minimal bactericidal concentrations (MBCs) were evaluated for the four antibiotics azithromycin, amoxicillin, ceftriaxone, and doxycycline against the three main genospecies of Borrelia burgdorferi sensu lato. In MBC testing, statistically significant differences between the genospecies could be found in 7 ou...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Sicklinger M,Wienecke R,Neubert U

    更新日期:2003-04-01 00:00:00

  • Intestinal colonization of symptomatic and asymptomatic schoolchildren with Blastocystis hominis.

    abstract::A study of single stool specimens was done to determine the prevalence of intestinal parasites among 1,000 primary school children. A questionnaire was completed by each child's parents. Specimens were examined by using wet-mount preparation, formaline-ether concentration, and Sheather's flotation technique. Trichrome...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Nimri L,Batchoun R

    更新日期:1994-11-01 00:00:00

  • Occurrence of Campylobacter jejuni in pets living with human patients infected with C. jejuni.

    abstract::Campylobacter jejuni was recovered from four dogs (11%) and four cats (33%) living with Danish human patients infected with C. jejuni. Pulsed-field gel electrophoresis (PFGE) analysis revealed the occurrence of the same quinolone-resistant strain in a girl and her dog. C. jejuni isolates with closely related (>95% sim...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Damborg P,Olsen KE,Møller Nielsen E,Guardabassi L

    更新日期:2004-03-01 00:00:00

  • Identification and type distribution of astroviruses among children with gastroenteritis in Colombia and Venezuela.

    abstract::Astrovirus infections were detected by enzyme immunoassay in 12 (5%) of 251 stool samples from children with gastroenteritis from Bogota, Colombia. In addition, astroviruses were detected by reverse transcription-PCR in 3 (10%) of 29 stool samples negative for other enteric pathogens collected in Caracas, Venezuela, f...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Medina SM,Gutierrez MF,Liprandi F,Ludert JE

    更新日期:2000-09-01 00:00:00

  • Serial quantitative PCR assay for detection, species discrimination, and quantification of Leishmania spp. in human samples.

    abstract::The Leishmania species cause a variety of human disease syndromes. Methods for diagnosis and species differentiation are insensitive and many require invasive sampling. Although quantitative PCR (qPCR) methods are reported for leishmania detection, no systematic method to quantify parasites and determine the species i...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Weirather JL,Jeronimo SM,Gautam S,Sundar S,Kang M,Kurtz MA,Haque R,Schriefer A,Talhari S,Carvalho EM,Donelson JE,Wilson ME

    更新日期:2011-11-01 00:00:00

  • "Campylobacter hyointestinalis" sp. nov.: a new species of Campylobacter found in the intestines of pigs and other animals.

    abstract::The name "Campylobacter hyointestinalis" sp. nov. is proposed for a Campylobacter species that was isolated from the intestines of pigs with proliferative enteritis. "C. hyointestinalis" is also found in the feces of cattle and has been isolated from the intestine of a hamster. "C. hyointestinalis" is distinguished fr...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Gebhart CJ,Edmonds P,Ward GE,Kurtz HJ,Brenner DJ

    更新日期:1985-05-01 00:00:00

  • Global multilocus sequence typing analysis of Mycoplasma bovis isolates reveals two main population clusters.

    abstract::Mycoplasma bovis is a major bovine pathogen associated with bovine respiratory disease complex and is responsible for substantial economic losses worldwide. M. bovis is also associated with other clinical presentations in cattle, including mastitis, otitis, arthritis, and reproductive disorders. To gain a better under...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Rosales RS,Churchward CP,Schnee C,Sachse K,Lysnyansky I,Catania S,Iob L,Ayling RD,Nicholas RA

    更新日期:2015-03-01 00:00:00

  • Validation of a microbead-based format for spoligotyping of Legionella pneumophila.

    abstract::A 42-plex clustered regularly interspaced short palindromic repeat (CRISPR)-based typing technique (spoligotyping) was recently developed at the French National Reference Center for Legionella. It allows the subtyping of the Legionella pneumophila sequence type 1/Paris pulsotype. In this report, we present the transfe...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Gomgnimbou MK,Ginevra C,Peron-Cane C,Versapuech M,Refrégier G,Jacotin N,Sola C,Jarraud S

    更新日期:2014-07-01 00:00:00

  • Evaluation of the clinical performance of the cobas 4800 HPV test in patients referred for colposcopy.

    abstract::The clinical performance of the cobas human papillomavirus (HPV) test for detection of high-grade disease in a colposcopy-referred population was compared with that of Hybrid Capture 2 (HC2). The overall agreement between the tests was 92.3%. Clinical sensitivity and specificity for detection of cervical intraepitheli...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: White C,Keegan H,Pilkington L,Ruttle C,Kerr P,Sharp L,O'Toole S,Turner M,Prendiville W,D'Arcy T,Fitzpatrick M,Lenehan P,Flannelly G,O'Leary JJ,Martin CM

    更新日期:2013-10-01 00:00:00

  • Comparative evaluation of microagglutination test and serum agglutination test as supplementary diagnostic methods for brucellosis.

    abstract::The diagnosis of brucellosis in cattle and small ruminants requires the use of more than one serological test. The complement fixation test (CFT), the rose bengal test (RBT), and the serum agglutination test (SAT) are among the most useful tests for routine diagnosis. The microagglutination test (MAT) was developed as...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Baum M,Zamir O,Bergman-Rios R,Katz E,Beider Z,Cohen A,Banai M

    更新日期:1995-08-01 00:00:00

  • Use of PCR and reverse line blot hybridization assay for rapid simultaneous detection and serovar identification of Chlamydia trachomatis.

    abstract::The aim of this study was to develop and evaluate multiplex and nested PCR-reverse line blot (RLB) hybridization assays for detection and serovar identification of Chlamydia trachomatis. Two sets of primers targeting the VD2 region of the omp1 gene and one set targeting the cryptic plasmid were designed for use in mul...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Xiong L,Kong F,Zhou H,Gilbert GL

    更新日期:2006-04-01 00:00:00

  • Rapid detection of methicillin-resistant Staphylococcus aureus strains not identified by slide agglutination tests.

    abstract::Seventy-nine methicillin-resistant Staphylococcus aureus (MRSA) strains, isolated during 1980 to 1990, were classified as MRSA Aggl- (14 strains) and MRSA Aggl+ (65 strains) strains on the basis of test results in slide agglutination assays designed to detect fibrinogen-binding protein (clumping factor) and protein A ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Kuusela P,Hildén P,Savolainen K,Vuento M,Lyytikäinen O,Vuopio-Varkila J

    更新日期:1994-01-01 00:00:00

  • Development of a rapid enzyme immunoassay for Clostridium difficile toxin A and its use in the diagnosis of C. difficile-associated disease.

    abstract::A rapid (2.5 h) direct enzyme immunoassay (EIA) for Clostridium difficile toxin A was developed for clinical use. Specimen centrifugation and filtration were not required. The EIA detected toxin A levels in patient stool as low as 20 pg (2 ng/ml of stool). The test was 5,000 times more sensitive for toxin A than it wa...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: DiPersio JR,Varga FJ,Conwell DL,Kraft JA,Kozak KJ,Willis DH

    更新日期:1991-12-01 00:00:00

  • Laboratory and clinical characteristics of Staphylococcus lugdunensis prosthetic joint infections.

    abstract::Staphylococcus lugdunensis is a coagulase-negative staphylococcus that has several similarities to Staphylococcus aureus. S. lugdunensis is increasingly being recognized as a cause of prosthetic joint infection (PJI). The goal of the present retrospective cohort study was to determine the laboratory and clinical chara...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Shah NB,Osmon DR,Fadel H,Patel R,Kohner PC,Steckelberg JM,Mabry T,Berbari EF

    更新日期:2010-05-01 00:00:00

  • Serodiagnosis of Porphyromonas gingivalis infection by immunoblot analysis with recombinant collagenase.

    abstract::The Porphyromonas gingivalis collagenase-specific serum immunoglobulin A (IgA), IgM, and IgG responses from 20 patients with early-onset periodontitis (EOP), 20 patients with adult periodontitis, (AP), and 20 age- and sex-matched healthy controls were examined by immunoblot analysis. A recombinant collagenase antigen ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Wittstock M,Flemmig TF,Schmidt H,Mutters R,Karch H

    更新日期:1996-10-01 00:00:00

  • Culture-Based Methods and Molecular Tools for Azole-Resistant Aspergillus fumigatus Detection in a Belgian University Hospital.

    abstract::Azole-resistant Aspergillus fumigatus is an increasing worldwide problem with major clinical implications. Surveillance is warranted to guide clinicians to provide optimal treatment to patients. To investigate azole resistance in clinical Aspergillus isolates in our institution, a Belgian university hospital, we condu...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Montesinos I,Argudín MA,Hites M,Ahajjam F,Dodémont M,Dagyaran C,Bakkali M,Etienne I,Jacobs F,Knoop C,Patteet S,Lagrou K

    更新日期:2017-08-01 00:00:00

  • Evidence of an epidemic of Blastocystis hominis infections in preschool children in northern Jordan.

    abstract::Blastocystis hominis is now gaining acceptance as an agent of human intestinal disease. A case-control study of the cause of gastroenteritis in children less than 6 years old was conducted. A total of 500 stool specimens were examined by wet mount preparation, formalin-ether concentration, Sheather's sugar flotation t...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Nimri LF

    更新日期:1993-10-01 00:00:00

  • Prevalence of blood-borne infectious diseases in blood donors in Ghana.

    abstract::Transfusion-transmissible infections among 808 blood donors in Ghana were investigated in 1999. Antibody seroprevalences of 3.8, 0.7, 8.4, and 13.5%, respectively, for human immunodeficiency virus, human T-cell lymphotrophic virus type 1, hepatitis C virus (HCV), and Treponema pallidum were obtained. The seroprevalenc...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Ampofo W,Nii-Trebi N,Ansah J,Abe K,Naito H,Aidoo S,Nuvor V,Brandful J,Yamamoto N,Ofori-Adjei D,Ishikawa K

    更新日期:2002-09-01 00:00:00

  • Disseminated infection with Mycobacterium tilburgii in a male immunocompromised patient.

    abstract::Mycobacterium tilburgii is a nonculturable nontuberculous mycobacterium identifiable only by molecular methods. We report a case of disseminated M. tilburgii infection illustrating the importance of 16S rRNA gene sequencing to determine the responsible mycobacterial pathogen and the difficulties in tailoring antimycob...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Temmerman S,Vandekerckhove L,Sermijn E,Vogelaers D,Claeys G,Vaneechoutte M,Cools P,Callens S

    更新日期:2014-05-01 00:00:00

  • Antibody to a 39-kilodalton Borrelia burgdorferi antigen (P39) as a marker for infection in experimentally and naturally inoculated animals.

    abstract::Borrelia burgdorferi expresses a conserved, species-specific 39-kDa protein (P39) that can stimulate antibodies during human infection. To confirm that anti-P39 antibodies are produced consistently in animals exposed to infectious spirochetes, white-footed mice, Peromyscus leucopus, and laboratory white mice, Mus musc...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Simpson WJ,Burgdorfer W,Schrumpf ME,Karstens RH,Schwan TG

    更新日期:1991-02-01 00:00:00

  • Methicillin-Resistant Staphylococcus aureus spa Type t002 Outbreak in Horses and Staff at a Veterinary Teaching Hospital after Its Presumed Introduction by a Veterinarian.

    abstract::Methicillin-resistant Staphylococcus aureus (MRSA) infection and colonization, involving MRSA strains which differ from common human health care-associated clones, have become serious emerging conditions in equine veterinary hospitals. In 2010, MRSA spa type t535 caused an outbreak involving both horses and personnel ...

    journal_title:Journal of clinical microbiology

    pub_type: 杂志文章


    authors: Steinman A,Masarwa S,Tirosh-Levy S,Gleser D,Kelmer G,Adler A,Carmeli Y,Schwaber MJ

    更新日期:2015-09-01 00:00:00